Background and Aim: The animal reservoir of leptospirosis is comprised of both domestic and wild mammals, with rats known as the most important in the spread of the disease. The occurrence of this reservoir in residential areas increases the potential for leptospirosis transmission. This study aimed to investigate the type of reservoirs and estimate the prevalence of leptospirosis in rats and livestock animals in Bantul and Gunungkidul districts, Special Region of Yogyakarta Province, Indonesia. Materials and Methods: This research utilized a cross-sectional study design. Rat trapping and livestock (cattle, goat, and sheep) blood surveys were conducted at four locations in each district. Samples of rat renal and livestock blood serum were examined using the polymerase chain reaction technique to determine the presence of Leptospira bacteria. The data were analyzed descriptively by describing the species of rats trapped, the types of cattle, and the prevalence of Leptospira in the sample. Results: The rat species infected with Leptospira in Bantul district consisted of Rattus tanezumi 4.8% (3/63); Rattus norvegicus 12.5% (2/16); Bandicota indica 28.6% (2/7); and Bandicota bengalensis 50.0% (1/2). No rats were found to be positive for Leptospira in Gunungkidul district. The prevalence of Leptospira in cattle was 63.64% (7/11) in Bantul district and 50.00% (8/16) in Gunungkidul district. In goats and sheep, the prevalence of Leptospira was 22.22% (2/9) in Bantul district and 45.16% (14/31) in Gunungkidul district. Conclusion: The potential exists for transmission of leptospirosis from rats and cattle in Bantul and Gunungkidul Districts. It is necessary to increase leptospirosis awareness. Community education, especially for livestock farmers, needs to be improved to prevent the transmission of leptospirosis from livestock.

The amount of e-wallet providers in Indonesia is so many, so there is intense competition between each e-wallet provider. The tighter competition for e-wallet, makes users not only loyal to one type of e-wallet and makes users switch brands or providers in using an e-wallet. This study aims to investigate the factors that influence e-service quality on e-loyalty mediated by e-trust and brand image. This research is categorized as explanatory research. Respondents in this study were users of the OVO e-wallet application in Malang City, determining the number of samples in this study using a purposive sampling technique with a sample of 200 respondents. The method of collecting data is using a questionnaire, the data is analyzed using PLS-SEM. The results showed that e-service quality had no significant effect on e-loyalty. E-service quality has a significant effect on e-trust, e-trust has a significant effect on e-loyalty. E-service quality has a significant effect on brand image, brand image has a significant effect on e-loyalty. E-trust and brand image can play a fully mediating role in the relationship between e-service quality and e-loyalty. OVO is expected to continue to maintain and increase e-trust because it is proven in this research that e-trust has the greatest influence on e-loyalty so that it can be a competitive advantage for OVO. Keywords: e-service quality, e-loyalty, e-trust, brand image.

This study aims to validate the dimensions of sharia experience quality and to determine the effect of sharia experience quality on customer loyalty by customer satisfaction as mediation variable. The sample technique of this study was a purposive sampling of 150 respondents who conducted financing at Islamic banks. SEM-PLS is used to test hypotheses. The results of the second order evaluation show that integration quality, physical environment quality, outcome quality, compliance sharia knowledge, and responsive religious experience had strong effects on sharia experience quality. Sharia experience quality had a significant effect on customer loyalty and customer satisfaction as a partial mediation of sharia experience quality on customer loyalty.

PENGEMBANGAN LEMBAR KERJA SISWA BERBASIS DISCOVERY LEARNING BERBANTUAN PhET INTERACTIVE SIMULATIONS PADA MATERI HUKUM NEWTON

Background and Aim: Rhodamine-B (Rh-B) marking shows a great potential for use in mark-release-recapture (MRR) studies for rear-and-release mosquito control strategies, including the radiation-based sterile insect technique. However, its applicability and evaluation in body-stain-irradiated males of Aedes aegypti have received little attention. The present study evaluated the use of Rh-B to mark gamma-irradiated male A. aegypti. Materials and Methods: Male A. aegypti were irradiated at the pupal stage at a dose of 70 Gy. After emergence, males were fed 0.1, 0.2, 0.3, or 0.4% Rh-B in 10% glucose solution for 4 days. Groups of unirradiated males that received the same feeding treatments were used as control groups. We evaluated the persistence of Rh-B and the longevity of males after Rh-B feeding. Furthermore, the use of Rh-B in irradiated A. aegypti for MRR experiments was evaluated at an urban site. Results: No difference was observed in the Rh-B persistence among all concentrations at the 24-h postmarking period ranging from 91.25 ± 1.61% to 96.25 ± 1.61% and from 90.00 ± 2.28% to 93.13 ± 2.77% for the unirradiated and irradiated groups, respectively. Rh-B persistence significantly decreased over time, and persistence was significantly longer with increased concentrations in both the unirradiated and irradiated groups. Longevity was considerably decreased by Rh-B feeding and irradiation. However, no significant difference in longevity was found among males fed various concentrations of Rh-B. Through MRR experiments, irradiated-Rh-B marked males were mostly detected within a radius of 20 m and 40 m from the center-release point. The mean distance traveled of the released males from the three MRR events was calculated to be 42.6 m. Conclusion: This study confirms that Rh-B body marking through sugar feeding is applicable for irradiated male A. aegypti, with only a slight effect on longevity. Furthermore, considering the significant reduction in persistence over time, further study is needed to assess the impact of this reduction on the calculation of field biological parameters resulting from MRR experiments. Keywords: irradiated males, mark-release-recapture, rhodamine-B, sterile insect technique.