Doxorubicin (DOX), a potent chemotherapy agent, useful in the treatment of solid tumors, lymphomas, and leukemias, is limited by its potentially lethal cardiotoxicity. However, exercise has been consistently shown to mitigate the side effects of DOX, including cardiotoxicity. To date, most studies examining the relationship between exercise and DOX-induced cardiotoxicity have focused on aerobic exercise, with very few examining the role of anerobic activity. Therefore, this investigation explored the potential of creatine (CR) and resistance training (RT) in preserving cardiac health during DOX therapy. Male Sprague-Dawley rats were grouped into RT, RT + CR, sedentary (SED), and SED + CR, with each division further branching into saline (SAL) or DOX-treated subsets post-10 weeks of RT or SED activity. RT comprised progressive training utilizing specialized cages for bipedal stance feeding. CR-treated groups ingested water mixed with 1% CR monohydrate and 5% dextrose, while control animals received 5% dextrose. At week 10, DOX was administered (2 mg/kg/week) over 4-weeks to an 8 mg/kg cumulative dose. Cardiac function post-DOX treatment was assessed via transthoracic echocardiography. Left ventricular diameter during diastole was lower in DOX + CR, RT + DOX, and RT + CR + DOX compared to SED + DOX (p < 0.05). Additionally, cardiac mass was significantly greater in RT + CR + DOX SED + DOX animals (p < 0.05). These results suggest RT and CR supplementation, separately and in combination, could attenuate some measures of DOX-induced cardiotoxicity and may offer a cost-effective way to complement cancer treatments and enhance patient outcomes. More investigations are essential to better understand CR’s prolonged effects during DOX therapy and its clinical implications.

It is widely accepted that pathophysiological changes to the central nervous system of persons with Parkinson’s disease (PD) result in negative effects on motor function. However, less information is known regarding the pathology of PD on skeletal muscle. The purpose of this study was to determine the effect of a fatiguing isometric knee extension protocol on muscle mechanics using evoked twitch contractions in persons with PD and in non-impaired older adults (OLD). Evoked twitch contractions were examined during a fatiguing protocol in PD (66 ± 9 yr, n = 8) and OLD (65 ± 10 yr, n = 5). Participants performed 5-sec maximal isometric voluntary contractions of the quadriceps femoris with 5-sec rest for 3-min. Every 30-sec during rest intervals, a maximal transcutaneous electrical stimulus was administered to the quadriceps femoris to quantify evoked peak twitch torque (pTT), peak relaxation rate (pRR), and peak rate of torque development (pRTD). A large effect of voluntary fatigue (%decline) was observed (g = 1.58). There were no significant differences in pTT (p = 0.09; 95% CI:-3.6, 0.28) or pRR (p = 0.11; 95% CI:-31, 3.6). However, the slope decline of pRTD in OLD (-35.4 ± 24.7) was greater than PD (-11.5 ± 11.4; p = 0.03), indicating that skeletal muscle in persons with PD is less fatigable compared to non-impaired older adults. The rate, not the maximum capacity, of torque generation of the muscle during a fatiguing knee extension protocol was affected by PD. Future studies are warranted to identify the mechanism(s) responsible for the observed differences in skeletal muscle contractile characteristics and potential myofiber distribution variation in PD.

This study examined the influence of muscle activation and motor unit number estimation (MUNE) on early phase voluntary rate of torque development (RTD) in younger (YM) and older (OM) men. Thirty-two YM (n = 17; Age = 22 yrs) and OM (n = 15; Age = 74 yrs) volunteered to participate in this study. Early phase RTD (first 50 ms of a rapid isometric contraction; RTD₅₀) and normalized surface electromyography (first 50 ms of muscle excitation; nEMG₅₀) were recorded from the right quadricep muscle group. MUNE was examined from the right vastus lateralis. Multiple linear regression analyses revealed that nEMG₅₀ had a significant effect on RTD₅₀ independent of age group (p ≤ 0.001). nEMG₅₀ had a significant effect on RTD₅₀ in the OM group (p = 0.037). MUNE had no effect on RTD₅₀ independent of age. Older adults may depend more on muscle activation at contraction onset of early phase RTD compared to younger adults.

This study investigated the effect of a commercially available ketone supplement on heart rate (HR), perceived exertion (RPE), blood lactate, glucose, and ketone concentrations, along with time to fatigue (TTF) during a running task to voluntary fatigue. Twelve NCAA Division I cross-country athletes took part in this randomized, double-blind, placebo-controlled cross-over study. Bayesian methodologies were employed for all statistical analyses, and point estimates were determined to be statistically significant if the 95% highest-density intervals (HDI) excluded zero. TTF was not significantly different between conditions with a Mean_diff = 48.7 ± 6.3 s (95% HDI: −335, 424) and a 0.39 probability derived from the posterior distribution, indicating the likelihood that the supplement would increase TTF compared to the placebo control. Lactate concentrations immediately post-exercise were significantly lower in the supplement trial relative to placebo with an estimated Mean_diff = −4.6 ± 1.9 mmol; 95% HDI: −8.3, −0.9. There were no significant interaction effects observed for either blood glucose or ketone concentrations nor HR or RPE. These findings imply that the acute ingestion of ketones before running at lactate threshold pace has a low probability of increasing TTF in highly trained Division I runners.

Background: There is a lack of research investigating the effect of hydration status or intermuscular glycogen content on ultrasound-derived muscle quality imaging in younger adults. Purpose: To examine the effects of varied hydration and muscle glycogen status on ultrasound-measured vastus lateralis (VL) cross-sectional area (CSA) and echo intensity (EI). Materials and Methods: Thirteen young adults (Age: 23 ± 4.2 yrs; Height: 168.5 ± 6.8 cm; Weight: 76.2 ± 17.1 kg) participated in five experimental visits: (1) Baseline (regular dietary intake); (2) hypohydrated and normal carbohydrate (CHO) intake (HH-NC); (3) euhydrated and normal CHO intake (EH-NC); (4) hypohydrated and CHO restriction (HH-CR); and (5) euhydrated and CHO restriction (EH-CR) conditions. Ultrasound imaging of the right VL was used to assess CSA and EI at each visit. A two-way repeated measures ANOVA with Holm adjusted post hoc test was performed for statistical analysis. Results: Body mass was affected by both hydration and carbohydrate restriction (both p < 0.001), while Usg was greater during hypohydration (p < 0.001). VLCSA was lower following carbohydrate restriction (p = 0.010), independent of hydration status, whereas VLEI was not significantly affected by hydration, carbohydrate restriction, or their interaction (all p ≥ 0.063). Conclusions: Overall, these findings suggest that an individual’s hydration and CHO intake may influence ultrasound-derived muscle CSA assessments. Future research should continue to explore the sensitivity of EI and CSA to determine the impact of these findings in both clinical and research environments.