Introduction and objective: Cancer is one of the major health problems today. In Poland, mortality due to cancer remains at 260 deaths per 100,000 population per year, and lung cancer accounts for the highest number of cancer deaths in men and women. Non-small cell lung cancer (NSCLC) accounts...

Stromal vascular fraction (SVF) isolated from adipose tissue is an important source of starting material for adipose tissue-derived advanced therapy medicinal products (ATMPs), but the influence of donor-related and harvesting-related factors on SVF isolation outcomes remains unclear. In this study, SVF was isolated from adipose tissue samples obtained from 100 adult donors undergoing elective liposuction using a standardized enzymatic digestion protocol. Donor age, body mass index (BMI), waist-to-hip ratio (WHR), and harvesting site were evaluated in relation to SVF yield, isolation efficiency, and cell viability using correlation and non-parametric statistical analyses. SVF was successfully isolated from all samples. Mean SVF yield was 16.7 ± 9.68 × 10⁶ cells per 100 mL of adipose tissue, and mean viability was 80.9 ± 9.97%. No significant associations were observed between donor age and SVF yield, isolation efficiency, or viability. Similarly, BMI and WHR were not significantly associated with SVF isolation outcomes, although BMI showed a weak positive trend with isolation efficiency (ρ = 0.181, p = 0.086). Harvesting site had no significant effect on isolation outcomes despite numerically higher yield and viability in thigh-derived samples. Initial SVF viability was not associated with subsequent ADSC yield or viability after culture. Overall, demographic, anthropometric, and harvesting-site characteristics showed limited influence on quantitative SVF isolation outcomes. These findings support the robustness of standardized SVF isolation procedures and indicate that adipose tissue represents a reliable source of starting material for adipose tissue-derived ATMP manufacturing.

Introduction and objective: The aim of this study was to determine the changes in vitamin D levels in patients before and during the COVID-19 pandemic, taking into account the effect of season. Vitamin D is one of the most important lipid-soluble vitamins. The maintenance of concentrations at...