Early fertilizer nitrogen (N) application on cover crops or their residues during the off-season is a practice adopted in Brazil subtropical conditions under no-tillage corn (Zea mays L.) systems. However, the effect of early N application on yield, plant N content, and N recovery efficiency (NRE) for corn is not yet well documented. Five fertilizer N timings in an oat-corn system were evaluated in two studies utilizing an isotopic-labeled N determination, ¹⁵N isotope. The N fertilization timings were: (i) oat tillering, (ii) 15 days before corn planting time, over the oat residues, (iii) at corn planting time, (iv) in-season at the three-leaf growth stage (V3), and (v) in-season split application at V3 and six-leaf (V6) growth stages. Based on the statistical analysis, the N fertilization timings were separated into three groups: 1) N-OATS, designated to N applied at oat; 2) N-PLANT, referred to pre-plant and planting N applications; and 3) N-CORN, designated to in-season corn N applications. Corn yield was not affected by the N fertilization timing. However, the N-CORN N fertilization timings enhanced NRE by 17% and 35% and final N recovery system (plant plus soil) by 16% and 24% all relative to N-OATS and N-PLANT groups, respectively. Overall, N-OATS resulted in the largest N derived from fertilizer (NDFF) amount in the deeper soil layer, in overall a delta of 10 kg N ha^-1 relative to the rest of the groups. Notwithstanding corn yield was not affected, early N fertilization under subtropical conditions is not a viable option since NRE was diminished and the non-recovery N increased relative to the in-season N applications.

The corn biomass and nutrient dynamics may be altered when it is intercropped with Brachiaria (syn. Urochloa spp.). The present study aimed to investigate the dynamics of biomass, nitrogen (N), phosphorus (P) and potassium (K) for farming systems that produce corn intercropped with Brachiaria species. Field experiments were performed during the season and off-season, in a split-plot design. The main plots were composed of Brachiaria species (B. brizantha,B. ruziziensis and B. Convert) intercropped with corn, in addition to corn monocropping. The subplots consisted of three forage sampling periods, ranging from 0 to 60 days after the corn harvest. The intercropping arrangements did not affect the corn grain yield, nutrient accumulation and partitioning, relatively to the corn monocropping. After the grain harvest, B. brizantha achieved the greater biomass accumulation rate in both the season (69 kg ha^-1 day^-1) and off-season (17 kg ha^-1 day^-1). The nutrient accumulation ranged widely between the Brachiaria species and planting seasons: 0.2-1.2 kg ha^-1 day^-1 for N; 0.01-0.07 kg ha^-1 day^-1 for P; and 0.13-0.8 kg ha^-1 day^-1 for K. However, the greatest nutrient accumulation was found for B. brizantha, followed by B. ruziziensis and then B. Convert. In the short-term, corn intercropped with Brachiaria in the season showed the largest effect on the nutrient cycling and biomass yield. The intercropping between corn and B. brizantha in the season was the best way to enhance the biomass yield and the N, P and K cycling.

Knowing the nutrient removal by soybean grain harvest in different varieties, locations, and over time is essential to correctly adjust agronomic recommendations, update farmers’ practices, and increase nutrient use efficiency. A field-based research trial was carried out to assess macronutrients [nitrogen (N), phosphorus (P), potassium (K), magnesium (Mg), calcium (Ca), and sulfur (S)] removed in grain by modern soybean varieties from southern Brazil introduced between 2007 and 2016. We examined changes between our set of modern varieties and a dataset of historical values encompassing a wide range of varieties introduced before 2007. Moreover, we undertook a synthesis analysis using scientific literature published after 2007 to investigate nutrient removal by grain among modern Brazilian soybeans and a dataset that included field trials from Argentina, United States, and India. There were no yield gains across the years for modern soybean varieties introduced among 2007 and 2016 in Brazil, although the grain N and Mg concentrations decreased. Modern Brazilian soybeans increased nutrient removal compared with that by soybeans historically planted in Brazil, with 11.1, 26.9, 45.0, and 31.6% more N, P, K, and Mg removed, respectively. Our results indicated that soybean growing in Brazil removed 4.3% less N relative to the values reported in the literature dataset, whereas K removal was 21.4% greater. A significant difference was also recorded for high-yield soybean varieties, and Brazilian varieties removed 11.8% less N and 8.6% more K than varieties in the literature dataset. No differences were found among locations for P removal, averaging 4.9 kg Mg^–1grain. In conclusion, this study indicates that the amounts of nutrients removed by modern soybean varieties were greater relative to the historical values recorded in Brazil, excluding Ca and S. Nonetheless, in the middle to long term (10 years), a significant impact of plant breeding on grain nutrient concentration was recorded only for N and Mg. The difference in nutrient removal patterns between Brazil and other countries indicates an integrated effect of management, genotype, and environment on nutrient removal. These findings provide guidance for optimal nutrient management and specific information for plant breeding programs to understand nutrient variability.

The objective of this work was to evaluate methods of intercropping corn and Panicum spp. forages and their effects on corn yield and forage development. Two experiments with Panicum spp. were conducted, one with 'Tanzania' and other with 'Massai' in the municipality of Piracicaba, in the state of São Paulo, Brazil, in the 2012/2013 growing season. In both experiments, the treatments were: broadcast seeding of Panicum spp. at the same time of corn sowing; corn with Panicum spp. between rows; Panicum spp. sown with fertilizer; Panicum spp. sown between corn rows after corn establishment; broadcast seeding of Panicum spp. after corn establishment; and sole corn sowing. For corn, leaf nitrogen content, ear height, and grain yield were evaluated. For Panicum spp. cultivars, height, dry mass, tiller density, and leaf:stem ratio were evaluated. The intercropping establishment methods used do not affect corn growth, grain yield, and N leaf content. The seeding of both cultivars of Panicum spp. when corn plants had four expanded leaves reduces forage dry mass production and increases the leaf:stem ratio. The Panicum spp. broadcast method, performed after corn was established, does not allow appropriate establishment, with few plants in the area.