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Shangyong Li
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Representative Publications
View All Publications7 May 2020 in Food & Function
Journal Article
Gut microbiota plays an important role in the high-fat diet (HFD)-induced obesity and related metabolic syndrome (MetS). Our previous study has demonstrated that unsaturated alginate oligosaccharides (UAOS) degraded by alginate lyase possess significant anti-obesity effects in HFD-fed mice. Herein, we further established that UAOS could significantly ameliorate obesity-related metabolic abnormalities, including hyperlipidemia, insulin resistance and low-grade inflammation. Particularly, the beneficial effect of UAOS on these metabolic abnormalities could be significantly reversed by antibiotic supplementation. Subsequently, the microbiological analysis has revealed that UAOS treatment can modulate the overall composition of the gut microbiota, which is highly associated with metabolic parameters. UAOS supplementation can partially reverse the gut dysbiosis induced by HFD-diet or antibiotics. Specifically, UAOS treatment selectively increased the relative abundance of beneficial intestinal bacteria (e.g. Lactobacillus and Akkermansia genus) and decreased the abundance of inflammogenic bacteria (e.g. Bacteroides and Parabacteroides). These results suggest that UAOS can attenuate the HFD-induced obesity and related abnormalities through modulating gut microbiota, indicating that UAOS can act as potent prebiotic agents in treating obesity and related metabolic diseases.
12 October 2020 in Food & Function
Journal Article
Ningning HeSWShuo Wang
Shuo Wang
ZLZhiyuan Lv
Zhiyuan Lv
WZWandong Zhao
Wandong Zhao
SLShangyong Li
Shangyong LiIn this study, the two enzymatic low molecular weight chitosan oligosaccharides (LMW-COSs), LMW-COS-H and LMW-COS-L, were prepared with average MWs of 879.6 Da and 360.9 Da, respectively. Compared to LMW-COS-L, the LMW-COS-H was more effective in improving high-fat diet (HFD)-induced metabolic abnormalities, such as obesity, hyperlipidemia, low-grade inflammation and insulin resistance. The subsequent analysis of gut microbiota showed that the supplement of LMW-COSs caused overall structural and genus/species-specific changes in the gut microbiota, which were significantly correlated with the metabolic parameters. Specifically, both of the LMW-COSs significantly decreased the relative abundance of inflammatory bacteria such as Erysipelatoclostridium and Alistipes, whereas that of the beneficial intestinal bacteria (such as Akkermansia and Gammaproteobacteria) increased significantly. This study suggested that there were potential prebiotic functions of LMW-COSs in HFD fed mice, which regulated the dysfunctional gut microbiota, alleviated low-grade inflammation and maintained the intestinal epithelial barrier.
8 February 2025 in Metabolic Brain Disease
Journal Article
CLCunyin Li
Cunyin Li
JYJingtong Yao
Jingtong Yao
CYChang Yang
Chang Yang
SYShengnan Yu
Shengnan Yu
ZYZizhen Yang
Zizhen Yang
Ningning He +2
13 October 2022 in Frontiers in Immunology
Journal Article
KCKaiwei Chen
Kaiwei Chen
SSShipeng Shang
Shipeng Shang
SYShengnan Yu
Shengnan Yu
LCLuwen Cui
Luwen Cui
SLShangyong Li
Shangyong LiNingning HeUlcerative colitis (UC) is a chronic inflammatory bowel disease (IBD). Its etiology is unclear. Much evidence suggests that the death of abnormal intestinal epithelial cells (IECs) leads to intestinal barrier disruption, and the subsequent inflammatory response plays a vital role in UC. Pyroptosis is a form of programmed inflammatory cell death, and the role of pyroptosis in UC etiology remains to be explored. This study identified 10 hub genes in pyroptosis by gene expression profiles obtained from the GSE87466 dataset. Meanwhile, the biomarkers were screened based on gene significance (GS) and module membership (MM) through the Weighted Gene Co-Expression Network Analysis (WGCNA). The following analysis indicated that hub genes were closely associated with the UC progression and therapeutic drug response. The single-cell RNA (scRNA) sequencing data from UC patients within the GSE162335 dataset indicated that macrophages were most related to pyroptosis. Finally, the expression of hub genes and response to the therapeutic drug [5-aminosalicylic acid (5-ASA)] were verified in dextran sulfate sodium (DSS)-induced colitis mice. Our study identified IL1B as the critical pyroptosis-related biomarker in UC. The crosstalk between macrophage pyroptosis and IEC pyroptosis may play an essential role in UC, deserving further exploration.
Journal Article
YWYanan Wang
Yanan WangXCXuehong Chen
Xuehong ChenXBXiaolin Bi
Xiaolin BiYRYining Ren
Yining RenQHQi Han
Qi HanSLShangyong Li
Shangyong Li +3
Alginate oligosaccharides (AOS) show versatile bioactivities. Although various alginate lyases have been characterized, enzymes with special characteristics are still rare. In this study, a polysaccharide lyase family 7 (PL7) alginate lyase-encoding gene, aly08, was cloned from the marine bacterium Vibrio sp. SY01 and expressed in Escherichia coli. The purified alginate lyase Aly08, with a molecular weight of 35 kDa, showed a specific activity of 841 U/mg at its optimal pH (pH 8.35) and temperature (45 °C). Aly08 showed good pH-stability, as it remained more than 80% of its initial activity in a wide pH range (4.0–10.0). Aly08 was also a thermo-tolerant enzyme that recovered 70.8% of its initial activity following heat shock treatment for 5 min. This study also demonstrated that Aly08 is a polyG-preferred enzyme. Furthermore, Aly08 degraded alginates into disaccharides and trisaccharides in an endo-manner. Its thermo-tolerance and pH-stable properties make Aly08 a good candidate for further applications.
23 May 2022 in Food & Function
Journal Article
NLNian Liu
Nian Liu
HWHaoyu Wang
Haoyu Wang
ZYZizhen Yang
Zizhen Yang
KZKunyi Zhao
Kunyi Zhao
SLShangyong Li
Shangyong LiNingning HeAn overview of the definitions, functions, mechanisms and applications of functional oligosaccharides in colitis prevention and treatment.
About Me
In June 2015, I obtained a medical doctoral degree from the School of Medicine, Ocean University of China. I was a postdoctoral fellow at the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences; I was a visiting Scholar at Sooming Women's University in South Korea, September 2019–October 2020, and a Research Professor at the Korea Research Foundation. Since August 2017, I am a lecturer, associate professor, distinguished professor, and master's supervisor in special medicine at the Department of Medical Pharmacology, School of Basic Medicine, Qingdao University. I currently serve as the Vice Dean of the School of Basic Medicine at Qingdao University.
I have published more than 20 papers included in SCI as the first/corresponding author (including as parallel first author), and have been cited more than 500 times. These include 16 papers in the first and second regions of the Chinese Academy of Sciences, such as J Agr Food Chem, Bioresour Technol, Food Chem, Food Function, etc. I have had 8 national invention patents and 1 international PCT patent authorized. I served as the Topic Editor for the SCI journal Front Bioeng Biotech. I have hosted over 10 projects, including the National Natural Science Foundation (NSFC), the Korean Research Foundation. My research interests include the development and mechanisms of action of prebiotics targeting gut microbiota, and research on the relationship between chronic inflammation and metabolic diseases.