Catharanthus roseus is a plant of the Apocynaceae family. It produces over 120 alkaloids, 70 of which are pharmacologically active. C. roseus produces vinblastine, utilized in treating Hodgkin’s disease; testicular tumors, breast carcinoma, choriocarcinoma, Kaposi sarcoma and Letterer-Siwe disorder. Vincristine is used to treat acute lymphocytic leukemia, lymphosarcoma, lympho-granulomatosis and in solid infant tumors. The preparation process of 1 kg of vincristine has a cost of US$ 3.5 million, while vinblastine has a cost of US$1 million. Therefore, 530 kg of dry leaves are necessary to produce 1 kg of vincristine and half a ton for getting 1 g of vinblastine. The high cost is due to the low concentrations in the aerial portion. Due to the high market value and its effectiveness in different medical treatments, this chapter deals with the pharmacological application of the C. roseus alkaloids.

Background: In some regions of Mexico, edible wild plants have been displaced or eliminated from the traditional food systems, mainly by changes in land use, booming monoculture, herbicide use, and by changes among the new generations in the traditional foods and diets of indigenous populations. In the Totonacapan region of Puebla, the gradual change from the traditional acahual plantation to coffee-type agroecosystems has provoked the displacement of old-growth forests and the eradication of wild plants since 1970. One of the wild species which has been used in traditional medicine and food recipes by the Totonac culture isSmilax aristolochiifolia(SMILACACEAE), known as “kgentsililh”. This species forms part of traditional Totonac recipes, in which the tender stems are still used in local medicine to treat menstrual pain, deal with dysentery, and prevent hair loss. According to the Maxent® Program, there are still potential areas with habitats suitable to promote its conservation in the Poblano Totonacapan.Methods: We conducted 260 interviews with people in 13 locations in the northern Sierra of the State of Puebla. Variables taken into account in the interview related to the consumption frequency of the species, its abundance and distribution perception, reasons or arguments given by the Totonac indigenous population about the decreased presence of specimens ofS. aristolochiifolia, its dates of collection, and the cutting prices of kgentsililh at the community level and in local markets. The relative abundance ofS. aristolochiifoliawas determined through 22 samples in 2ts of 600 m². Later, its potential distribution in the state of Puebla was estimated using the Maxent® Program Ver. 3.3.3.Results: Of the 260 Totonac families interviewed, 31% had stopped consuming kgentsililh. The residents reported that in the last 50 years the populations of this plant had diminished in the northern Sierra of the State of Puebla, mainly due to changes in land use, herbicide application, over-collection, and urban growth. In traditional medicine, the stem sap ofS. aristolochiifoliais currently employed to help treat baldness, and the “tuberous root” or plant rhizome is used to prepare a tea infusion to treat dysentery. The cost of plant guides varies from 10.00 to 40.00 Mexican pesos for one bunch (around 0.5 to 2.00 US dollars), and every bundle consists of between 7 to 10 cuttings from 0.4 to 0.5 m long. From our 22 quadrats of sampling and collection ofS. aristolochiifolia, we were able to recognize a total of 32 specimens. There is a considerable abundance of kgentsililh in acahual plantations and old-growth forests (evergreen lowland and mid-elevation perennial forest) concerning the coffee-type plantations and milpas. According to our analysis using the Maxent Program®, eight physical and climatic variables have a direct relationship to the potential distribution of the species.Conclusions: Smilax aristolochiifoliais still a plant of socioeconomic importance, mainly because of its food value and its use in traditional medicine by indigenous families in Poblano Totonacapan. It is evident that the villagers perceive that in the last 50 years the species has decreased its population mainly due to land-use change, the application of herbicides to the different family production units, and climate change. At the moment, there is no knowledge about the methods of propagation of the species, and therefore there is no intention on the part of the population to conserve the species. However, it would be of great importance to generate a biocultural conservation strategy and take advantage of the results obtained from the potential geographic distribution area, since according to the Maxent® Program, there are still potential areas with habitat suitable to promote conservation in Poblano Totonacapan.

This systematic determination of morphological and phytochemical data was conducted with the purpose of conserving and identifying the phylogenetic relationship among the Vanilla species of the Totonacapan region in Mexico to increase awareness of the genetic biodiversity. Samples of Vanilla planifolia, V. planifolia cv. "oreja de burro", V. pompona, V. insignis, and V. inodora, are distributed across 19 municipalities of the State of Veracruz and 19 municipalities of the State of Puebla. Morphological data parameters were determined in situ and included leaf length, leaf width, leaf thickness, stem diameter, stem thickness, node distance, stem texture degree, flower colour intensity, and fruit length. Similarly, alkaloids, tannins, saponins, phenols, flavonoids, and terpenes were determined by specifically phytochemical tests and quantified by thin layer chromatography. Both, morphological and phytochemical data parameters, were successfully used in assembling dendrograms by using the Euclidian distance method and by principal component analysis.

Objetivo: caracterizar el patrón alimentario de las familias campesinas y determinar la influencia de los factores socioeconómicos en el origen y consumo de los alimentos en el Totonacapan poblano (México). Metodología: con la técnica de recordatorio de 24 horas, se aplicó una encuesta a 270 familias sobre su dieta y se documentó la información socioeconómica. Resultados: se registraron 159 alimentos, de los cuales 57.6% tiene procedencia externa e incluyen grasas, lácteos, huevo, pan, pasta de trigo, sal y alimentos y bebidas altas en azúcares. El maíz, algunos frijoles y chiles, las hojas verdes “quelites”, las frutas, la carne de pollo y el puerco proceden en gran medida de la producción propia, local y regional. Valor: se aportan datos sobre la dieta campesina actual y sus implicaciones para la agrodiversidad y la salud. Conclusiones: la dieta campesina se está desligando de la producción de autoconsumo debido a la migración asociada con bajos ingresos.

Peasant societies have traditionally produced food for themselves and for the market based on a diversity of plants that they grow and cultivate in their agroecosystems; however, these societies are modifying their agriculture, their consumption, the structure and composition of their plots and abandoning the consumption of these species, which are gradually ceasing to be part of their diets. This research aimed to analyze the contribution of local crop diversity to the peasant diet of the Totonacapan region of Puebla, Mexico. During 2020, 270 dietary surveys were applied, and in 2022, the richness of edible species in 146 peasant plots was recorded and 69 semi-structured interviews were conducted to document ethnobotanical information on edible species. A total of 102 edible species were identified in the plots; 65 are native and 37 are introduced. The milpas and the family garden are the main areas where food for self-consumption is grown: corn, beans, and some grean leaves (quelites). Meanwhile, coffee plantations and horticultural areas mainly contain food for sale; coffee, fat pepper, bananas, oranges, and chili peppers stand out. Half of the plants inventoried (53%) were not recorded in the diet surveys. Absent foods were fruit trees, roots and tubers, spices, quelites, and local vegetables. On the other hand, most of the 48 species recorded in the plots and the dietary surveys had a very low frequency of consumption. The limited consumption of this group of species is largely because they are no longer suitable for consumption, are difficult to cook, or require much time for collection and preparation. The reason villagers conserve these plants may be because they are emergency foods. After all, they consume them eventually or in times of scarcity, hence the importance of keeping them in the plots. Even though a great wealth of edible plants is grown in the campesino plots, it does not mean they have a relevant presence in the diets.