Anaerobic ammonium oxidation (Anammox) is a cost-effective process for treating highly nitrogenous wastewater. However, the fate of organic nitrogen during Anammox treatment is still unclear, which limits its practical application. In this work, the changes in the quality of dissolved organic nitrogen (DON) in coal liquefaction wastewater (CLW) during Anammox were studied in relation to its chemical composition, which was determined by Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). The molecular-level characterization of extracellular polymeric substances (EPS) in the Anammox sludge is also reported for the first time in this paper. The relative contribution of N-containing compounds to the total dissolved organic matter (DOM) determined by summating the normalized intensities exceeded 30%, highlighting the complexity of the nitrogenous compounds in the influent. Additionally, Anammox appeared to be better suited to removing DON compounds with fewer carbonyl or carboxyl groups, more aromatic structures, and higher oxidative properties. Lignin-like substances were verified as the predominant component of N-containing compounds in Anammox EPS, followed by protein and substances with condensed aromatic structures. DON compounds with higher degrees of saturation, lower molecular weight, and higher lignin-like properties were more prone to absorption by Anammox EPS. A series of microbe-mediated pathways were demonstrated to be responsible for DON biodegradation, which revealed the organic and inorganic nitrogen removal mechanisms in the Anammox reactor. The obtained results provide great support to the ongoing efforts to optimize the Anammox process.

Removal of steroid hormones from aqueous environment is of prevailing concern because of their adverse impact on organisms. Using biochar derived from biomass as adsorbent to remove pollutants has become more popular due to its low cost, effectiveness, and sustainability. This study evaluated the feasibility of applying corn straw biochar (CSB) and dewatered sludge biochar (DSB) to reduce 17β-estradiol (E2) from aquatic solutions by adsorption. The experimental results showed that the adsorption kinetics and isotherm behavior of E2 on the two biochars were well described by the pseudo-second-order (R² > 0.93) and Langmuir models (R² > 0.97). CSB has higher E2 adsorption capacity than DSB, and the maximum adsorption capacity was 99.8 mg/g obtained from Langmuir model at 298 K, which can be attributed to the higher surface area, porosity, and hydrophobicity of this adsorbent. Higher pH levels (>10.2) decreased the adsorption capacities of biochar for E2, while the ionic strength did not significantly affect the adsorption process. The regeneration ability of CSB was slightly better than that of DSB. The possible adsorption mechanism for E2 on biochar is suggested as π–π interactions, H–bonding, and micropores filling. These results indicated that CSB has more potential and application value than DSB on reducing E2 from aqueous solutions when considering economy and removal performance.