Background: Skin glycation, oxidation, carbonylation, and excessive inflammation are well‐recognized factors contributing to skin aging and pigmentation. Previous in vitro studies have confirmed vitamin C's antioxidant, anti‐glycation, and anti‐inflammatory properties, but its in vivo effects remain to be further verified. Aims: To investigate the in vivo effects of topical vitamin C serum on skin anti‐glycation, anti‐carbonylation, antioxidation, and anti‐inflammation, and to provide evidence for its anti‐aging and skin‐brightening applications. Patients/Methods: A randomized, double‐blind, controlled trial was conducted, enrolling 66 healthy Chinese females, with 31 in the blank group and 35 in the topical group. Participants applied 10% vitamin C serum for 12 weeks. Skin glycation, carbonylated protein content, interleukin‐1α (IL‐1α) levels, free radical scavenging capacity, and skin color parameters were evaluated before and after treatment. Results: After 12 weeks, the topical group showed significant improvements: AGEs reduced by 17.65%, carbonylation fluorescence intensity decreased by 49.22%, IL‐1α content dropped by 58.73%, and ABTS free radical scavenging rate increased by 12.14%. Skin yellowness (b* value) and redness (a* value) decreased by 6.13% and 16.46%, respectively (all p < 0.001). Conclusions: Topical 10% vitamin C serum can effectively mitigate skin glycation, carbonylation, and inflammation, enhance skin antioxidant capacity, and improve skin color, supporting its clinical value in anti‐aging and skin‐brightening.