Through analyzing the concentrations of selected heavy metals (Ba, Mn, Pb, Sr, Zn) in the bones and teeth of wild living and ecologically equivalent ruminants from the Tian-Shan (Capra sibirica and Ovis ammon polii) and the West Carpathians (Rupicapra rupicapra tatrica) we compared the environmental pollution levels of these two mountain ranges. The samples were analyzed by X-ray fluorescence. Significantly higher contents of Zn and Mn as well as a higher frequency of measurable occurrences of Mn, Ba, and Pb in samples from the West Carpathians confirmed the results of our previous study, that the West Carpathians are relatively more polluted by heavy metals than the Tian-Shan Mountains. The most probably contamination sources are mining and smelting as well as traffic emissions, which can reach remote mountain ranges through long distance atmospheric transport.

We compared the content of lead (Pb) and other trace metals in various bone and tooth tissues of two alpine rodent species with different behaviours. Hibernating marmots accumulated less Pb than the non-hibernating and shorter-lived snow voles. It was confirmed that calcium (Ca) is substituted by Pb in Tatra marmot teeth and skull bones. Ca concentration in marmot bones is likely maintained even during this inactive period and this mechanism may reduce Pb accumulation in bones of hibernating marmots. Pb accumulation in bone and tooth tissues may be slightly mitigated by zinc content and also by increased strontium (Sr) in bones. Trace metals deposited differently in tooth and skull bone tissues of marmots. Frontal and parietal skull bones contained significantly more Pb than marmot incisors, but the difference was not significant when comparing skull bones with molars. Molars are static and could reflect past exposure of heavy metals, whereas incisors renew themselves, and would not.

High mountain areas are an appropriate indicator of anthropogenic lead (Pb), which can reach remote mountain ranges through long distance atmospheric transport. We compared the content of Pb in ecologically equivalent rodent species from Tian-Shan with European mountain ranges including the Tatra, Vitosha and Rila mountains. We used bone tissues from terminal tail vertebrae of small rodents for detection of Pb levels through electro-thermal atomic absorption spectroscopy (AAS). The tailbones of Tian-Shan rodents had significantly lower Pb levels than snow voles from the Tatra Mountains, but there was no significant difference in comparison with the Vitosha and Rila mountains. We can conclude that Tian-Shan shows lower pollution by Pb than the Tatras, which may be a result of prolonged industrialization of north-western Europe and strongly prevailing west winds in this region.

The trilateral biosphere reserve in the Eastern Carpathians borders Poland, Slovakia and Ukraine and represents a key model for conserving biological diversity with respect to socio-economic and sustainable development. It is the regional representative of much diversity (language, history, culture, legal framework, and land use and management), and ecological studies are more accessible than data on the mutual effect of nature conservation on the local economy. Our study compares demography, land use, revenue of local stakeholders and the development of eco-tourism using data from the Slovakian and Ukrainian UNESCO World Heritage regions, where both Slovak and Ukrainian stakeholders profit mainly from the forestry industry, though additional but small incomes are derived from local recreation. Incomes from tourism are higher in Ukraine than in Slovakia, while in Slovakia significantly higher income comes in from local taxes than in Ukraine. These factors lead to depopulation (especially of young people) and marginalization of nature conservancy in the region. The forestry industry, with few and less sophisticated job opportunities, and a generally negative impact on the environment (intensive logging, soil erosion, etc.), casually suppresses the creation and development of eco-tourism essentials to the region. To mitigate this effect, this study suggests several steps toward the development of eco-tourism in the Eastern Carpathians-Poloniny region.