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Amino acids have been implicated with virus infection and replication. Here, we demonstrate the effects of two basic amino acids, arginine and lysine, and their ester derivatives on infection of two enveloped viruses, SARS-CoV-2, and influenza A virus. We found that lysine and its ester derivative can efficiently block infection of both viruses in vitro. Furthermore, the arginine ester derivative caused a significant boost in virus infection. Studies on their mechanism of action revealed that the compounds potentially disturb virus uncoating rather than virus attachment and endosomal acidification. Our findings suggest that lysine supplementation and the reduction of arginine-rich food intake can be considered as prophylactic and therapeutic regimens against these viruses while also providing a paradigm for the development of broad-spectrum antivirals.
Ivonne Melano; Li-Lan Kuo; Yan-Chung Lo; Po-Wei Sung; Ni Tien; Wen-Chi Su. Effects of Basic Amino Acids and Their Derivatives on SARS-CoV-2 and Influenza-A Virus Infection. Viruses 2021, 13, 1301 .
AMA StyleIvonne Melano, Li-Lan Kuo, Yan-Chung Lo, Po-Wei Sung, Ni Tien, Wen-Chi Su. Effects of Basic Amino Acids and Their Derivatives on SARS-CoV-2 and Influenza-A Virus Infection. Viruses. 2021; 13 (7):1301.
Chicago/Turabian StyleIvonne Melano; Li-Lan Kuo; Yan-Chung Lo; Po-Wei Sung; Ni Tien; Wen-Chi Su. 2021. "Effects of Basic Amino Acids and Their Derivatives on SARS-CoV-2 and Influenza-A Virus Infection." Viruses 13, no. 7: 1301.
Targeting the activities of endoplasmic reticulum (ER)–mitochondrial-dependent metabolic reprogramming is considered one of the most promising strategies for cancer treatment. Here, we present biochemical subcellular fractionation, coimmunoprecipitation, gene manipulation, and pharmacologic evidence that induction of mitochondria-localized phospho (p)-cyclin dependent kinase 1 (CDK1) (Thr 161)–cyclin B1 complexes by apigenin in nasopharyngeal carcinoma (NPC) cells impairs the ER–mitochondrial bioenergetics and redox regulation of calcium (Ca++) homeostasis through suppressing the B cell lymphoma 2 (BCL-2)/BCL-2/B-cell lymphoma-extra large (BCL-xL)-modulated anti-apoptotic and metabolic functions. Using a specific inducer, inhibitor, or short hairpin RNA for acid sphingomyelinase (ASM) demonstrated that enhanced lipid raft-associated ASM activity confers alteration of the lipid composition of lipid raft membranes, which leads to perturbation of protein trafficking, and induces formation of p110α free p85α–unphosphorylated phosphatase and tensin homolog deleted from chromosome 10 complexes in the lipid raft membranes, causing disruption of phosphatidylinositol 3-kinase (PI3K)−protein kinase B (Akt)−GTP-ras-related C3 botulinum toxin substrate 1 (Rac1)-mediated signaling, thus triggering the p-CDK1 (Thr 161))–cyclin B1-mediated BCL-2 (Thr 69/Ser 87)/BCL-xL (Ser 62) phosphorylation and accompanying impairment of ER–mitochondria-regulated bioenergetic, redox, and Ca++ homeostasis. Inhibition of apigenin-induced reactive oxygen species (ROS) generation by a ROS scavenger N-acetyl-L-cysteine blocked the lipid raft membrane localization and activation of ASM and formation of ceramide-enriched lipid raft membranes, returned PI3K−Akt−GTP-Rac1-modulated CDK1–cyclin B1 activity, and subsequently restored the BCL-2/BCL-xL-regulated ER–mitochondrial bioenergetic activity. Thus, this study reveals a novel molecular mechanism of the pro-apoptotic activity of ASM controlled by oxidative stress to modulate the ER–mitochondrial bioenergetic metabolism, as well as suggests the disruption of CDK1–cyclin B1-mediated BCL-2/BCL-xL oncogenic activity by triggering oxidative stress−ASM-induced PI3K−Akt−GTP-Rac1 inactivation as a therapeutic approach for NPC.
Jan-Gowth Chang; Ni Tien; Yi-Chih Chang; Meng-Liang Lin; Shih-Shun Chen. Oxidative Stress-Induced Unscheduled CDK1–Cyclin B1 Activity Impairs ER–Mitochondria-Mediated Bioenergetic Metabolism. Cells 2021, 10, 1280 .
AMA StyleJan-Gowth Chang, Ni Tien, Yi-Chih Chang, Meng-Liang Lin, Shih-Shun Chen. Oxidative Stress-Induced Unscheduled CDK1–Cyclin B1 Activity Impairs ER–Mitochondria-Mediated Bioenergetic Metabolism. Cells. 2021; 10 (6):1280.
Chicago/Turabian StyleJan-Gowth Chang; Ni Tien; Yi-Chih Chang; Meng-Liang Lin; Shih-Shun Chen. 2021. "Oxidative Stress-Induced Unscheduled CDK1–Cyclin B1 Activity Impairs ER–Mitochondria-Mediated Bioenergetic Metabolism." Cells 10, no. 6: 1280.
Malaria is an infectious disease caused by Plasmodium parasites that are mainly transmitted through the bites of infected female Anopheles mosquitoes. The average annual number of malaria cases was less than ten in Taiwan in the last five years. Most of the cases were caused by Plasmodium vivax and Plasmodium falciparum, and were primarily diagnosed in travelers who returned from Southeast Asia and Africa. Here, we report the first case of Plasmodium ovale infection within five years that was confirmed by peripheral blood smear examination and molecular identification in a 25-year-old Asian female patient who returned from Uganda.
Yu-Chuan Tseng; Yu-Chang Chang; Chihyi Lee; Shih-Fen Hsu; Pai-Chuan Chang; Jen-Jen Hsu; Peter Bor-Chian Lin; Min-Chi Lu; Ni Tien; Chiung-Tzu Hsiao. Molecular diagnosis and therapy for Plasmodium ovale infection of a returned traveler from East Africa. Journal of the Formosan Medical Association 2021, 1 .
AMA StyleYu-Chuan Tseng, Yu-Chang Chang, Chihyi Lee, Shih-Fen Hsu, Pai-Chuan Chang, Jen-Jen Hsu, Peter Bor-Chian Lin, Min-Chi Lu, Ni Tien, Chiung-Tzu Hsiao. Molecular diagnosis and therapy for Plasmodium ovale infection of a returned traveler from East Africa. Journal of the Formosan Medical Association. 2021; ():1.
Chicago/Turabian StyleYu-Chuan Tseng; Yu-Chang Chang; Chihyi Lee; Shih-Fen Hsu; Pai-Chuan Chang; Jen-Jen Hsu; Peter Bor-Chian Lin; Min-Chi Lu; Ni Tien; Chiung-Tzu Hsiao. 2021. "Molecular diagnosis and therapy for Plasmodium ovale infection of a returned traveler from East Africa." Journal of the Formosan Medical Association , no. : 1.
(1) Background: surface-enhanced Raman spectroscopy (SERS) is a novel method for bacteria identification. However, reported applications of SERS in clinical diagnosis are limited. In this study, we used cylindrical SERS chips to detect urine pathogens in urinary tract infection (UTI) patients. (2) Methods: Urine samples were retrieved from 108 UTI patients. A 10 mL urine sample was sent to conventional bacterial culture as a reference. Another 10 mL urine sample was loaded on a SERS chip for bacteria identification and antibiotic susceptibility. We concentrated the urine specimen if the intensity of the Raman spectrum required enhancement. The resulting Raman spectrum was analyzed by a recognition software to compare with spectrum-form reference bacteria and was further confirmed by principal component analysis (PCA). (3) Results: There were 97 samples with single bacteria species identified by conventional urine culture and, among them, 93 can be successfully identified by using SERS without sample concentration. There were four samples that needed concentration for bacteria identification. Antibiotic susceptibility can also be found by SERS. There were seven mixed flora infections found by conventional culture, which can only be identified by the PCA method. (4) Conclusions: SERS can be used in the diagnosis of urinary tract infection with the aid of the recognition software and PCA.
Ni Tien; Tzu-Hsien Lin; Zen-Chao Hung; Hsiu-Shen Lin; I-Kuan Wang; Hung-Chih Chen; Chiz-Tzung Chang. Diagnosis of Bacterial Pathogens in the Urine of Urinary-Tract-Infection Patients Using Surface-Enhanced Raman Spectroscopy. Molecules 2018, 23, 3374 .
AMA StyleNi Tien, Tzu-Hsien Lin, Zen-Chao Hung, Hsiu-Shen Lin, I-Kuan Wang, Hung-Chih Chen, Chiz-Tzung Chang. Diagnosis of Bacterial Pathogens in the Urine of Urinary-Tract-Infection Patients Using Surface-Enhanced Raman Spectroscopy. Molecules. 2018; 23 (12):3374.
Chicago/Turabian StyleNi Tien; Tzu-Hsien Lin; Zen-Chao Hung; Hsiu-Shen Lin; I-Kuan Wang; Hung-Chih Chen; Chiz-Tzung Chang. 2018. "Diagnosis of Bacterial Pathogens in the Urine of Urinary-Tract-Infection Patients Using Surface-Enhanced Raman Spectroscopy." Molecules 23, no. 12: 3374.
Background The Campylobacter species usually causes infection between humans and livestock interaction via livestock breeding. The studies of the Campylobacter species thus far in all clinical isolates were to show the many kinds of antibiotic phenomenon that were produced. Their integrons cause the induction of antibiotic resistance between bacterial species in the Campylobacter species. Results The bacterial strains from the diarrhea of pediatric patient which isolated by China Medical University Hospital storage bank. These isolates were identified by MALDI-TOF mass spectrometry. The anti-microbial susceptibility test showed that Campylobacter species resistant to cefepime, streptomycin, tobramycin and trimethoprim/sulfamethoxazole (all C. jejuni and C. coli isolates), ampicillin (89% of C. jejuni; 75% of C. coli), cefotaxime (78% of C. jejuni; 100% of C. coli), nalidixic acid (78% of C. jejuni; 100% of C. coli), tetracycline (89% of C. jejuni; 25% C. coli), ciprofloxacin (67% of C. jejuni; 50% C. coli), kanamycin (33% of C. jejuni; 75% C. coli) and the C. fetus isolate resisted to ampicillin, cefotaxime, nalidixic acid, tetracycline, ciprofloxacin, kanamycin by disc-diffusion method. The effect for ciprofloxacin and tetracycline of the Campylobacter species was tested using an E-test. The tet, erm, and integron genes were detected by PCR assay. According to the sequencing analysis (type I: dfr12-gcuF-aadA2 genes and type II: dfrA7 gene), the cassette type was identified. The most common gene cassette type (type I: 9 C. jejuni and 2 C. coli isolates; type II: 1 C. coli isolates) was found in 12 class I integrase-positive isolates. Conclusions Our results suggested an important information in the latency of Campylobacter species with resistance genes, and irrational antimicrobial use should be concerned.
Yi-Chih Chang; Ni Tien; Jai-Sing Yang; Chi-Cheng Lu; Fuu-Jen Tsai; Tsurng-Juhn Huang; I-Kuan Wang. Class 1 integrons and plasmid-mediated multiple resistance genes of the Campylobacter species from pediatric patient of a university hospital in Taiwan. Gut Pathogens 2017, 9, 50 .
AMA StyleYi-Chih Chang, Ni Tien, Jai-Sing Yang, Chi-Cheng Lu, Fuu-Jen Tsai, Tsurng-Juhn Huang, I-Kuan Wang. Class 1 integrons and plasmid-mediated multiple resistance genes of the Campylobacter species from pediatric patient of a university hospital in Taiwan. Gut Pathogens. 2017; 9 (1):50.
Chicago/Turabian StyleYi-Chih Chang; Ni Tien; Jai-Sing Yang; Chi-Cheng Lu; Fuu-Jen Tsai; Tsurng-Juhn Huang; I-Kuan Wang. 2017. "Class 1 integrons and plasmid-mediated multiple resistance genes of the Campylobacter species from pediatric patient of a university hospital in Taiwan." Gut Pathogens 9, no. 1: 50.
Clostridium difficile is a human and animal pathogen. Recently, the incidence of community-acquired C. difficile infection has increased, and many studies have indicated that C. difficile might be food-borne. The correlation between C. difficile infection in humans and in animals has been a topic of debate. The objective of this study was to determine the genetic relatedness of C. difficile from human and pigs in Taiwan. We investigated the molecular epidemiology of C. difficile in healthy humans and pigs from 2011 to 2015. The isolation rate of C. difficile from pigs in 13 commercial farms was 49% (100/204), and a high proportion of hypervirulent (C. difficile carrying tcdA, tcdB, and cdtA/B genes and a 39-bp deletion in the tcdC gene) ribotype 078 lineage isolates (90%, 90/100; including 078, 126, 127, and 066-like isolates) were identified. In addition, the C. difficile ribotype 127 isolates from pigs typically exhibited moxifloxacin resistance (37/43; 86%). In healthy humans, the isolation rate was 4.3% (3/69), and all healthy human isolates were non-toxigenic. In particular, we compared the porcine isolates with two patient strains (ribotype 127) obtained from two hospitals in central Taiwan. The multilocus variable number tandem repeat analysis revealed a high genetic relatedness between ribotype 127 from patients and pigs. This study indicated that isolates of the ribotype 078 lineage, and especially ribotype 127, were widely distributed in pig farms and showed a high frequency of moxifloxacin resistance. The closely related ribotype 127 from patients and pigs may have had a common origin or low diversity. In conclusion, C. difficile ribotype 127 is a noteworthy pathogen in pigs and poses a potential public health threat.
Ying-Chen Wu; Jen-Jie Lee; Bo-Yang Tsai; Yi-Fen Liu; Chih-Ming Chen; Ni Tien; Pei-Jane Tsai; Ter-Hsin Chen. Potentially hypervirulent Clostridium difficile PCR ribotype 078 lineage isolates in pigs and possible implications for humans in Taiwan. International Journal of Medical Microbiology 2016, 306, 115 -122.
AMA StyleYing-Chen Wu, Jen-Jie Lee, Bo-Yang Tsai, Yi-Fen Liu, Chih-Ming Chen, Ni Tien, Pei-Jane Tsai, Ter-Hsin Chen. Potentially hypervirulent Clostridium difficile PCR ribotype 078 lineage isolates in pigs and possible implications for humans in Taiwan. International Journal of Medical Microbiology. 2016; 306 (2):115-122.
Chicago/Turabian StyleYing-Chen Wu; Jen-Jie Lee; Bo-Yang Tsai; Yi-Fen Liu; Chih-Ming Chen; Ni Tien; Pei-Jane Tsai; Ter-Hsin Chen. 2016. "Potentially hypervirulent Clostridium difficile PCR ribotype 078 lineage isolates in pigs and possible implications for humans in Taiwan." International Journal of Medical Microbiology 306, no. 2: 115-122.
Yao-Ru Tseng; Tsen - Lu Cho; Kuei-Chu Li; Hsiu-Shen Lin; Kao-Pin Hwang; Ni Tien. Evaluation of a chlorine dioxide water treatment system to control Legionella species in a hospital water System. Journal of Microbiology, Immunology and Infection 2015, 48, S137 .
AMA StyleYao-Ru Tseng, Tsen - Lu Cho, Kuei-Chu Li, Hsiu-Shen Lin, Kao-Pin Hwang, Ni Tien. Evaluation of a chlorine dioxide water treatment system to control Legionella species in a hospital water System. Journal of Microbiology, Immunology and Infection. 2015; 48 (2):S137.
Chicago/Turabian StyleYao-Ru Tseng; Tsen - Lu Cho; Kuei-Chu Li; Hsiu-Shen Lin; Kao-Pin Hwang; Ni Tien. 2015. "Evaluation of a chlorine dioxide water treatment system to control Legionella species in a hospital water System." Journal of Microbiology, Immunology and Infection 48, no. 2: S137.
The aim of this study was to evaluate whether polymorphisms in the guanine nucleotide binding (G protein), beta polypeptide 1 (GNB1) gene are associated with a rapid virological response (RVR) among HCV genotype 1 (HCV-1) and 2 (HCV-2) infected patients receiving peginterferon plus ribavirin treatment (PEG-IFNα-RBV).
Yun-Ping Lim; Fuu-Jen Tsai; Wen-Ling Liao; Ni Tien; Dong-Zong Hung; Cheng-Yuan Peng; Lei Wan. Association of genetic variations in GNB1 with response to peginterferon plus ribavirin therapy for chronic hepatitis C in a Chinese population in Taiwan. BMC Gastroenterology 2012, 12, 167 -167.
AMA StyleYun-Ping Lim, Fuu-Jen Tsai, Wen-Ling Liao, Ni Tien, Dong-Zong Hung, Cheng-Yuan Peng, Lei Wan. Association of genetic variations in GNB1 with response to peginterferon plus ribavirin therapy for chronic hepatitis C in a Chinese population in Taiwan. BMC Gastroenterology. 2012; 12 (1):167-167.
Chicago/Turabian StyleYun-Ping Lim; Fuu-Jen Tsai; Wen-Ling Liao; Ni Tien; Dong-Zong Hung; Cheng-Yuan Peng; Lei Wan. 2012. "Association of genetic variations in GNB1 with response to peginterferon plus ribavirin therapy for chronic hepatitis C in a Chinese population in Taiwan." BMC Gastroenterology 12, no. 1: 167-167.
Ni Tien; Cheng-Mao Ho; Hui-Ju Lin; Mu-Chin Shih; Mao-Wang Ho; Hsiao-Chuan Lin; Hsiu-Shen Lin; Chao-Chin Chang; Jang-Jih Lu. Multilocus sequence typing of invasive group B Streptococcus in central area of Taiwan. Journal of Microbiology, Immunology and Infection 2011, 44, 430 -434.
AMA StyleNi Tien, Cheng-Mao Ho, Hui-Ju Lin, Mu-Chin Shih, Mao-Wang Ho, Hsiao-Chuan Lin, Hsiu-Shen Lin, Chao-Chin Chang, Jang-Jih Lu. Multilocus sequence typing of invasive group B Streptococcus in central area of Taiwan. Journal of Microbiology, Immunology and Infection. 2011; 44 (6):430-434.
Chicago/Turabian StyleNi Tien; Cheng-Mao Ho; Hui-Ju Lin; Mu-Chin Shih; Mao-Wang Ho; Hsiao-Chuan Lin; Hsiu-Shen Lin; Chao-Chin Chang; Jang-Jih Lu. 2011. "Multilocus sequence typing of invasive group B Streptococcus in central area of Taiwan." Journal of Microbiology, Immunology and Infection 44, no. 6: 430-434.