Background: In Malaysia, dengue remains a top priority disease and usage of insecticides is the main method for dengue vector control. Limited baseline insecticide resistance data in dengue hotspots has prompted us to conduct this study. The present study reports the use of a map on the insecticide susceptibility status of Aedes aegypti and Aedes albopictus to provide a quick visualization and overview of the distribution of insecticide resistance. Method and results: The insecticide resistance status of Aedes populations collected from 24 dengue hotspot areas from the period of December 2018 until June 2019 was proactively monitored using the World Health Organization standard protocol for adult and larval susceptibility testing was conducted, together with elucidation of the mechanisms involved in observed resistance. For resistance monitoring, susceptibility to three adulticides (permethrin, deltamethrin, and malathion) was tested, as well as susceptibility to the larvicide, temephos. Data showed significant resistance to both deltamethrin and permethrin (pyrethroid insecticides), and to malathion (organophosphate insecticide) in all sampled Aedes aegypti populations, while variable resistance patterns were found in the sampled Aedes albopictus populations. Temephos resistance was observed when larvae were tested using the diagnostic dosage of 0.012mg/L but not at the operational dosage of 1mg/L for both species. Conclusion: The present study highlights evidence of a potential threat to the effectiveness of insecticides currently used in dengue vector control, and the urgent requirement for insecticide resistance management to be integrated into the National Dengue Control Program.

A growing number of fast-food outlets in close proximity to residential areas raises a question as to its impact on childhood overweight and obesity. This study aimed at determining the relationship between the availability of fast-food outlets that were in close proximity to residential areas and overweight among Malaysian children aged 5 to 18 years. Measurement data on the weight and height of 5544 children (2797 boys, 2747 girls) were obtained from the National Health and Morbidity Survey 2011. Overweight (including obesity) is defined as BMI-for-age z-score > +1 SD based on the WHO growth reference. Geographic information system geospatial analysis was performed to determine the number of fast-food outlets within 1000 m radius from the children’s residential address. Multiple logistic regression was conducted to examine the association between the availability of fast-food outlets (none or more than one outlet) and overweight with adjustment for age, sex, ethnicity, monthly household income, parental educational level, residential area and supermarket density. Our results showed that the prevalence of overweight was 25.0% and there was a statistically significant association between the density of fast-food outlets and overweight (odds ratio: 1.23, 95% confidence interval: 1.03, 1.47). Our study suggested that the availability of fast-food outlets with close proximity in residential areas was significantly associated with being overweight among children. Limiting the number of fast-food outlets in residential areas could have a significant effect in reducing the prevalence of overweight among Malaysian children.

Dengue is endemic in Malaysia, and vector control strategies are vital to reduce dengue transmission. The Wolbachia strain wAlbB carried by both sexes of Ae. aegypti was released in Mentari Court, a high-rise residential site, in October 2017 and stopped after 20 weeks. Wolbachia frequencies are still being monitored at multiple traps across this site, providing an opportunity to examine the spatiotemporal distribution of Wolbachia and mosquito density with respect to year, residential block, and floor, using spatial interpolation in ArcGIS, GLMs, and contingency analyses. In just 12 weeks, Wolbachia-infected mosquitoes were established right across the Mentari Court site with an overall infection frequency of >90%. To date, the Wolbachia frequency of Ae. aegypti has remained high in all areas across the site despite releases finishing four years ago. Nevertheless, the Wolbachia invaded more rapidly in some residential blocks than others, and also showed a relatively higher frequency on the eighth floor. The Ae. aegypti index tended to differ somewhat between residential blocks, whilst the Ae. albopictus index was relatively higher at the top and bottom floors of buildings. In Mentari Court, only a short release period was required to infiltrate Wolbachia completely and stably into the natural population. The results inform future releases in comparable sites in a dengue control programme.

Background: Aedes aegypti is an important vector that transmits dengue, Zika, chikungunya, and yellow fever viruses. Although research on Aedes aegypti has been conducted for decades, scientometric studies on Aedes aegypti are scarce, are limited to regions, and cover short periods. Thus, there is still a knowledge gap in the current trend, research focuses and directions, leading authors and collaboration, journal and citation impacts, countries, and worldwide collaborations. Objective: The objectives of the study are to investigate the research trend, focus and directions, citation impact, leading authors and collaboration, journals, and countries of the published works on Aedes aegypti to inform the current knowledge gaps and future direction of the control of the vector. Methods: In this study, we searched the Scopus database for articles on Aedes aegypti published from the year 1927 until April 5th, 2024, and included articles, reviews, books, and book chapters that were written in English. A total of 16,247 articles in 160 journals with 481,479 citations were included. Inconsistencies in authors’ names were checked and cleaned using OpenRefine. The data were grouped into 4 periods; years 1927-1999, 2000-2009, 2010-2019, and 2020-2023. The relative growth rate and doubling time of publications were calculated. The analysis was conducted using VOSviewer, R bibliometrics, and citeSpace. Results: The overall RGR was 0.1. Doubling time increased from 9.3 in 1978-1998 to 12.1 in 2000-2009. The main research clusters were “using Wolbachia,” “Dengue Zika,” “worldwide diversity,” “community support,” “larvicidal activity,” “mosquito genotype-dependent,” and “sterile insect technique.” Journal of Medical Entomology was the leading journal (758/16,247, 4.7%). The most cited articles were authored by Halstead SB and team in Science (N=1355) and Kraemer MU and team in eLife (N=1324). The United States (5806/23,538, 24.7%) and Brazil (2035/23,538, 8.6%) were the top countries. Gubler DJ was the top co-cited author (n=2892) from 2000 to 2019. The co-cited author cluster patterns informed the significant specialty research on Aedes aegypti across time. Authors from various specialized research fields tended to collaborate across countries, especially neighboring countries. Countries with more research funding on the study of Aedes aegypti published more papers. Conclusions: Researchers or entomologists could understand the current knowledge gap on Aedes aegypti and plan for future research pathways. This study contributed to the public health stakeholders in improving the vector control interventions and elucidated the extent of research subject areas.

Zoonotic diseases such as malaria, dengue, Zika and chikungunya remain endemic in many countries. Setting and deploying trap to capture the host/vector species are fundamental to understand their density and distribution. Human effort to manage the trap data accurately and timely is an exhaustive endeavour when the study area expands and study period prolongs. One stop mobile app to manage and monitor the process of animal trapping, from field to laboratory level is still scarce. We developed a new mobile app named “PesTrapp” especially for medical entomologists to acquire the vector density index based on the mobile updates of ovitraps and species information in field and laboratory. This study aimed to highlight the mobile app’s development and design, describe the user experience and evaluate the preliminary user assessment of the mobile app. The mobile app was developed using mobile framework and database. User evaluation of the mobile app was based on the adjusted Mobile App Rating Scale and Standardized User Experience Percentile Rank Questionnaire. The process flows of system design and detailed screen layouts were described. The user experiences of using the app in a project to study Aedes surveillance in six study sites in Selangor, Malaysia were described. The overall mean user evaluation score of the mobile app was 4.0 (SD=0.6), which showed its acceptability by the users. The PesTrapp, a one-stop solution, is anticipated to improve the entomological surveillance work processes. This new mobile app can contribute as a tool in the vector control countermeasure strategies.

BACKGROUND Aedes aegypti is an important vector that transmits dengue, Zika, chikungunya, and yellow fever viruses. Although research on Aedes aegypti has been conducted for decades, scientometric studies on Aedes aegypti are scarce, are limited to regions, and cover short periods. Thus, there is still a knowledge gap in the current trend, research focuses and directions, leading authors and collaboration, journal and citation impacts, countries, and worldwide collaborations. OBJECTIVE The objectives of the study are to investigate the research trend, focus and directions, citation impact, leading authors and collaboration, journals, and countries of the published works on Aedes aegypti to inform the current knowledge gaps and future direction of the control of the vector. METHODS In this study, we searched the Scopus database for articles on Aedes aegypti published from the year 1927 until April 5th, 2024, and included articles, reviews, books, and book chapters that were written in English. A total of 16,247 articles in 160 journals with 481,479 citations were included. Inconsistencies in authors’ names were checked and cleaned using OpenRefine. The data were grouped into 4 periods; years 1927-1999, 2000-2009, 2010-2019, and 2020-2023. The relative growth rate and doubling time of publications were calculated. The analysis was conducted using VOSviewer, R bibliometrics, and citeSpace. RESULTS The overall RGR was 0.1. Doubling time increased from 9.3 in 1978-1998 to 12.1 in 2000-2009. The main research clusters were “using Wolbachia,” “Dengue Zika,” “worldwide diversity,” “community support,” “larvicidal activity,” “mosquito genotype-dependent,” and “sterile insect technique.” Journal of Medical Entomology was the leading journal (758/16,247, 4.7%). The most cited articles were authored by Halstead SB and team in Science (N=1355) and Kraemer MU and team in eLife (N=1324). The United States (5806/23,538, 24.7%) and Brazil (2035/23,538, 8.6%) were the top countries. Gubler DJ was the top co-cited author (n=2892) from 2000 to 2019. The co-cited author cluster patterns informed the significant specialty research on Aedes aegypti across time. Authors from various specialized research fields tended to collaborate across countries, especially neighboring countries. Countries with more research funding on the study of Aedes aegypti published more papers. CONCLUSIONS Researchers or entomologists could understand the current knowledge gap on Aedes aegypti and plan for future research pathways. This study contributed to the public health stakeholders in improving the vector control interventions and elucidated the extent of research subject areas. CLINICALTRIAL