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Asad Syed
Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia

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Journal article
Published: 04 October 2020 in International Journal of Environmental Research and Public Health
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Excessive use of fungicides in agriculture may result in substantial accumulation of active residues in soil, which affect crop health and yield. We investigated the response of Raphanus sativus (white radish) to fungicides in soil and potential beneficial interactions of radish plants with fungicide-tolerant plant growth-promoting rhizobacteria (PGPR). The PGPR were isolated from cabbage and mustard rhizospheres. Morphological and biochemical characteristics measured using standard methods, together with analysis of partial 16S rRNA gene sequences, revealed that fungicide-tolerant PGPR, isolates PS3 and AZ2, were closely related to Pseudomonas spp. These PGPR survived in the presence of high fungicide concentrations i.e., up to 2400 μg mL−1 carbendazim (CBZM) and 3200 μg mL−1 hexaconazole (HEXA). Bacterial isolates produced plant growth stimulants even under fungicide stress, though fungicides induced surface morphological distortion and alteration in membrane permeability of these bacteria, which was proved by a set of microscopic observations. Fungicides considerably affected the germination efficiency, growth, and physiological development of R. sativus, but these effects were relieved when inoculated with PGPR isolates. For instance, CBZM at 1500 mg kg−1 decreased whole dry biomass by 71%, whole plant length by 54%, total chlorophyll by 50%, protein content by 61%, and carotenoid production by 29%. After applying isolate AZ2 for white radish grown in CBZM (10 mg kg−1)-amended soil, it could improve plant growth and development with increased whole plant dry weight (10%), entire plant length (13%) and total chlorophyll content (18%). Similarly, isolate PS3 enhanced plant survival by relieving plant stress with declined biomarkers, i.e., proline (12%), malondialdehyde (3%), ascorbate peroxidase (6.5%), catalase (18%), and glutathione reductase (4%). Application of isolates AZ2 and PS3 could be effective for remediation of fungicide-contaminated soil and for improving the cultivation of radish plants while minimizing inputs of fungicides.

ACS Style

Sadaf Khan; Mohammad Shahid; Mohammad Saghir Khan; Asad Syed; Ali H. Bahkali; Abdallah M. Elgorban; John Pichtel. Fungicide-Tolerant Plant Growth-Promoting Rhizobacteria Mitigate Physiological Disruption of White Radish Caused by Fungicides Used in the Field Cultivation. International Journal of Environmental Research and Public Health 2020, 17, 7251 .

AMA Style

Sadaf Khan, Mohammad Shahid, Mohammad Saghir Khan, Asad Syed, Ali H. Bahkali, Abdallah M. Elgorban, John Pichtel. Fungicide-Tolerant Plant Growth-Promoting Rhizobacteria Mitigate Physiological Disruption of White Radish Caused by Fungicides Used in the Field Cultivation. International Journal of Environmental Research and Public Health. 2020; 17 (19):7251.

Chicago/Turabian Style

Sadaf Khan; Mohammad Shahid; Mohammad Saghir Khan; Asad Syed; Ali H. Bahkali; Abdallah M. Elgorban; John Pichtel. 2020. "Fungicide-Tolerant Plant Growth-Promoting Rhizobacteria Mitigate Physiological Disruption of White Radish Caused by Fungicides Used in the Field Cultivation." International Journal of Environmental Research and Public Health 17, no. 19: 7251.

Journal article
Published: 22 May 2020 in Nanomaterials
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Aminoglycosides (AMGs) have been extensively used to treat infectious diseases caused by Gram-negative bacteria in livestock and humans. A selective and sensitive colorimetric probe for the determination of streptomycin and kanamycin was proposed based on chlortetracycline-coated silver nanoparticles (AgNPs–CTC) as the sensing element. Almost all of the tested aminoglycoside antibiotics can rapidly induce the aggregation of AgNPs, along with a color change from yellow to orange/red. The selective detection of aminoglycoside antibiotics, including tobramycin, streptomycin, amikacin, gentamicin, neomycin, and kanamycin, with other types of antibiotics, can be achieved by ultraviolet (UV) spectroscopy. This developed colorimetric assay has ability to detect various AMGs using in-depth surface plasmon resonance (SPR) studies. With this determination of streptomycin and kanamycin was achieved at the picomolar level (pM) by using a UV–visible spectrophotometer. Under aqueous conditions, the linear range of the colorimetric sensor for streptomycin and kanamycin was 1000–1,1000 and 120–480 pM, respectively. The corresponding limit of detection was 2000 pM and 120 pM, respectively. Thus, the validated dual colorimetric and ratiometric method can find various analytical applications for the ultrasensitive and rapid detection of AMG antibiotics in water samples.

ACS Style

Ganesh Dattatraya Saratale; Rijuta Ganesh Saratale; Gajanan Ghodake; Surendra Shinde; Dae-Young Kim; Abdullah A. Alyousef; Mohammed Arshad; Asad Syed; Deepak Pant; Han-Seung Shin. Chlortetracycline-Functionalized Silver Nanoparticles as a Colorimetric Probe for Aminoglycosides: Ultrasensitive Determination of Kanamycin and Streptomycin. Nanomaterials 2020, 10, 997 .

AMA Style

Ganesh Dattatraya Saratale, Rijuta Ganesh Saratale, Gajanan Ghodake, Surendra Shinde, Dae-Young Kim, Abdullah A. Alyousef, Mohammed Arshad, Asad Syed, Deepak Pant, Han-Seung Shin. Chlortetracycline-Functionalized Silver Nanoparticles as a Colorimetric Probe for Aminoglycosides: Ultrasensitive Determination of Kanamycin and Streptomycin. Nanomaterials. 2020; 10 (5):997.

Chicago/Turabian Style

Ganesh Dattatraya Saratale; Rijuta Ganesh Saratale; Gajanan Ghodake; Surendra Shinde; Dae-Young Kim; Abdullah A. Alyousef; Mohammed Arshad; Asad Syed; Deepak Pant; Han-Seung Shin. 2020. "Chlortetracycline-Functionalized Silver Nanoparticles as a Colorimetric Probe for Aminoglycosides: Ultrasensitive Determination of Kanamycin and Streptomycin." Nanomaterials 10, no. 5: 997.