During their lifetime, plants encounter numerous biotic and abiotic stresses with diverse modes of attack. Phytohormones, including salicylic acid (SA), ethylene (ET), jasmonate (JA), abscisic acid (ABA), auxin (AUX), brassinosteroid (BR), gibberellic acid (GA), cytokinin (CK) and the recently identified strigolactones (SLs), orchestrate effective defense responses by activating defense gene expression. Genetic analysis of the model plant Arabidopsis thaliana has advanced our understanding of the function of these hormones. The SA- and ET/JA-mediated signaling pathways were thought to be the backbone of plant immune responses against biotic invaders, whereas ABA, auxin, BR, GA, CK and SL were considered to be involved in the plant immune response through modulating the SA-ET/JA signaling pathways. In general, the SA-mediated defense response plays a central role in local and systemic-acquired resistance (SAR) against biotrophic pathogens, such as Pseudomonas syringae, which colonize between the host cells by producing nutrient-absorbing structures while keeping the host alive. The ET/JA-mediated response contributes to the defense against necrotrophic pathogens, such as Botrytis cinerea, which invade and kill hosts to extract their nutrients. Increasing evidence indicates that the SA- and ET/JA-mediated defense response pathways are mutually antagonistic.

Agriculture is largely dependent on climate and is highly vulnerable to climate change. The global mean surface temperatures are increasing due to global climate change. Temperature beyond the physiological optimum for growth induces heat stress in plants causing detrimental and irreversible damage to plant development, growth, as well as productivity. Plants have evolved adaptive mechanisms in response to heat stress. The classical plant hormones, such as auxin, abscisic acid (ABA), brassinosteroids (BRs), cytokinin (CK), salicylic acid (SA), jasmonate (JA), and ethylene (ET), integrate environmental stimuli and endogenous signals to regulate plant defensive response to various abiotic stresses, including heat. Exogenous applications of those hormones prior or parallel to heat stress render plants more thermotolerant. In this review, we summarized the recent progress and current understanding of the roles of those phytohormones in defending plants against heat stress and the underlying signal transduction pathways. We also discussed the implication of the basic knowledge of hormone-regulated plant heat responsive mechanism to develop heat-resilient plants as an effective and efficient way to cope with global warming.

Callose is an important linear form of polysaccharide synthesized in plant cell walls. It is mainly composed of β-1,3-linked glucose residues with rare amount of β-1,6-linked branches. Callose can be detected in almost all plant tissues and are widely involved in various stages of plant growth and development. Callose is accumulated on plant cell plates, microspores, sieve plates, and plasmodesmata in cell walls and is inducible upon heavy metal treatment, pathogen invasion, and mechanical wounding. Callose in plant cells is synthesized by callose synthases located on the cell membrane. The chemical composition of callose and the components of callose synthases were once controversial until the application of molecular biology and genetics in the model plant Arabidopsis thaliana that led to the cloning of genes encoding synthases responsible for callose biosynthesis. This minireview summarizes the research progress of plant callose and its synthetizing enzymes in recent years to illustrate the important and versatile role of callose in plant life activities.

Protoplasts, derived from plant cells, exhibit remarkable totipotency and hold significant value across a wide spectrum of biological and biotechnological applications. These versatile applications encompass protein subcellular localization and interaction analysis, gene expression regulation, functional characterization, gene editing techniques, and single-cell sequencing. Protoplasts’ usability stems from their inherent accessibility and their ability to efficiently incorporate exogenous genes. In this review, we provide a comprehensive overview, including details on isolation procedures and influencing factors, purification and viability assessment methodologies, and the utilization of the protoplast transient expression system. The aim is to provide a comprehensive overview of current applications and offer valuable insights into protoplast isolation and the establishment of transient expression systems in a diverse range of plant species, thereby serving as a valuable resource for the plant science community.

Plant cells are separated by cellulose cell walls that impede direct cell-to-cell contact. In order to facilitate intercellular communication, plant cells develop unique cell-wall-spanning structures termed plasmodesmata (PD). PD are membranous channels that link the cytoplasm, plasma membranes, and endoplasmic reticulum of adjacent cells to provide cytoplasmic and membrane continuity for molecular trafficking. PD play important roles for the development and physiology of all plants. The structure and function of PD in the plant cell walls are highly dynamic and tightly regulated. Despite their importance, plasmodesmata are among the few plant cell organelles that remain poorly understood. The molecular properties of PD seem largely elusive or speculative. In this review, we firstly describe the general PD structure and its protein composition. We then discuss the recent progress in identification and characterization of PD-associated plant cell-wall proteins that regulate PD function, with particular emphasis on callose metabolizing and binding proteins, and protein kinases targeted to and around PD.

Rice tillering is an important characteristic that responds to both GA (gibberellin) and nitrogen-based fertilizers. How plants balance these two responses? A newly identified NGR5 (NITROGEN-MEDIATED TILLER GROWTH RESPONSE 5) protein reveals its important role in controlling the balance between GA-regulated dwarfism and nitrogen-regulated tillering. NGR5 directly interacts with PRC2 (Polycomb Repressive Complex 2) to form a repressive complex at the shoot branching inhibitory genes in nitrogen-dependent way, thereby repressing branching inhibitors and promoting tillering in response to nitrogen fertilizers. The GA receptor GID1 (GIBBERELLIN INSENSITIVE DWARF1) targets NGR5 for proteolysis by the 26S proteasome. The rice DELLA proteins of GA signaling way competitively inhibit GID1-NGR5 interaction, thereby protecting NGR5 from degradation and enhancing nitrogen-induced tiller number.