A label-free electrochemical method is developed for simple and convenient quantification of gene-specific DNA hypermethylation in a DNA sequence without PCR amplification, bisulfite conversion or labeling processes.

An efficient electrochemical approach is developed for ultrasensitive profiling of the methylation status of the p53 tumor suppressor gene based on a label-free biosensor in combination with bisulfite conversion.

A fluorescent probe (TPA–BTD–MT) was designed to monitor cyanide ions (CN⁻) with a “turn-on” response, changing from “turn-off” behavior due to the structural change. TPA–BTD–MT exhibited high selectivity for sensing CN⁻ in several food samples and was successfully used for imaging CN⁻ in living cells and animals with strong “turn-on” fluorescence.