Critical thinking, regarded as an indispensable high-order thinking for pre-service teachers, is of great significance in effective teaching and the cultivation of students’ critical thinking. Given that it has been confirmed by previous studies that the development of pre-service teachers’ critical thinking can be promoted by microteaching, a “Practice-Feedback-Journal” microteaching model was innovatively constructed in this study, aiming to cultivate the critical thinking of pre-service teachers. To investigate the impact of this model, 41 participants from a Chinese normal university were selected for a 12-week microteaching intervention experiment. Subsequently, content analysis of their online reflective journals was conducted using the Nvivo 20 software, based on the critical thinking framework. The findings suggest that the model has the potential to enhance Chinese pre-service teachers’ critical thinking. Evidence indicates that reflective feedback and case-based teaching strategies appear supportive, while cooperative learning also emerged as a promising approach due to its dual simulation of both student and teacher perspectives. As a small-scale exploratory study, this research offers preliminary insights and potential avenues for cultivating higher-order thinking in teacher education.