Climate change will increase drought in many regions of the world. Besides decreasing productivity, drought also decreases the concentration (%) of nitrogen (N) and phosphorous (P) in plants. We investigated if decreases in nutrient status during drought are correlated with decreases in levels of nutrient-uptake proteins in roots, which has not been quantified. Drought-sensitive (Hordeum vulgare, Zea mays) and -tolerant grasses (Andropogon gerardii) were harvested at mid and late drought, when we measured biomass, plant %N and P, root N- and P-uptake rates, and concentrations of major nutrient-uptake proteins in roots (NRT1 for NO₃, AMT1 for NH₄, and PHT1 for P). Drought reduced %N and P, indicating that it reduced nutrient acquisition more than growth. Decreases in P uptake with drought were correlated with decreases in both concentration and activity of P-uptake proteins, but decreases in N uptake were weakly correlated with levels of N-uptake proteins. Nutrient-uptake proteins per gram root decreased despite increases per gram total protein, because of the larger decreases in total protein per gram. Thus, drought-related decreases in nutrient concentration, especially %P, were likely caused, at least partly, by decreases in the concentration of root nutrient-uptake proteins in both drought-sensitive and -tolerant species.

Silicon (Si) is a plant beneficial element associated with the mitigation of abiotic and biotic stresses. Most greenhouse-grown ornamentals are considered low Si accumulators based on foliar Si concentration. However, Si accumulates in all tissues, and there is little published data on the distribution of Si in plants. This knowledge may be critical to using Si to mitigate tissue-specific plant stresses, e.g., pathogens. Therefore, we quantified Si accumulation and distribution in petunia ( Petunia × hybrida Hort. Vilm.-Andr. ‘Dreams Pink’), a low Si accumulator, and sunflower ( Helianthus annuus L. ‘Pacino Gold’), a high Si accumulator. Plants were grown in a sphagnum peat: perlite substrate amended with 0% (−Si) or 20% (+Si) parboiled rice hulls for 53 (petunia) or 72 days (sunflower). Aboveground dry weight was greater in nonamended petunia (13%) and sunflower (18%), compared with rice hull–amended plants, but days to flower was unaffected. Sunflowers grown in the rice hull–amended substrate had the greatest Si concentration in leaves (10,909 mg·kg ⁻¹ ), whereas roots (895 mg·kg ⁻¹ ), stems (303 mg·kg ⁻¹ ), and flowers (252 mg·kg ⁻¹ ) had lower, but similar Si concentrations. In petunia, Si concentration was greatest in leaves (2036 mg·kg ⁻¹ ), then roots (1237 mg·kg ⁻¹ ), followed by stems (301 mg·kg ⁻¹ ), and flowers (247 mg·kg ⁻¹ ). The addition of rice hulls to the substrate increased Si concentration in sunflower 414% in roots, 512% in flowers, 611% in stems, and 766% in leaves. By contrast, Si concentration in petunia increased only 7% in flowers, 105% in stems, and 115% in leaves, but increased 687% in roots. In rice hull–amended sunflowers, the distribution of Si was 91% in leaves, 3% in stems, 3% in roots, and 3% in flowers, and in petunia, it was 72% in leaves, 17% in stems, 6% in roots, and 5% in flowers.

Silicon (Si) is a plant-beneficial element that can alleviate the effects of abiotic and biotic stress. Plants are typically classified as Si accumulators based on foliar Si concentrations (≥1% Si on a dry weight basis for accumulators). By this definition, most greenhouse-grown ornamentals are low Si accumulators. However, plants that accumulate low foliar Si concentrations may still accumulate high Si concentrations elsewhere in the plant. Additionally, screening cultivars for variability in Si uptake has not been investigated for low Si accumulator species. Therefore, the objective of this study was to assess cultivar variability in Si accumulation and distribution in petunia ( Petunia ×hybrida ). Eight cultivars (Supertunia Black Cherry, Supertunia Limoncello, Supertunia Priscilla, Supertunia Raspberry Blast, Supertunia Royal Velvet, Supertunia Sangria Charm, Supertunia Vista Silverberry, and Supertunia White Improved) were grown in a commercial peat-based soilless substrate under typical greenhouse conditions. They were supplemented with either 2 m m potassium silicate (+Si) or potassium sulfate (-Si) at every irrigation. Silicon supplementation increased leaf dry mass (4.5%) but did not affect total dry mass. In plants not receiving Si supplementation, leaf Si ranged from 243 to 1295 mg·kg ⁻¹ , stem Si ranged from 48 to 380 mg·kg ⁻¹ , flower Si ranged from 97 to 437 mg·kg ⁻¹ , and root Si ranged from 103 to 653 mg·kg ⁻¹ . Silicon supplementation increased Si throughout the plant, but most predominantly in the roots. Leaf Si in the 2 m m Si treatment ranged from 1248 to 3541 mg·kg ⁻¹ (173% to 534% increase), stem Si ranged from 195 to 654 mg·kg ⁻¹ (72% to 376% increase), flower Si ranged from 253 to 1383 mg·kg ⁻¹ (74% to 1082% increase), and root Si ranged from 4018 to 10,457 mg·kg ⁻¹ (593% to 9161% increase). The large increase in root Si following supplementation shifted Si distribution within plants. In nonsupplemented plants, it ranged from 51.2% to 76.8% in leaves, 8.2% to 40.2% in stems, 2.8% to 23.8% in flowers, and 1.2% to 13.8% in roots. In Si-supplemented plants, it ranged from 63.5% to 67.7% in leaves, 10.5% to 22.6% in roots, 9.4% to 17.7% in stems, and 1.6% to 9.6% in flowers. This study indicates that petunia, a low foliar Si accumulator, can accumulate appreciable quantities of Si in roots when provided supplemental Si.

The response of plant N relations to the combination of elevated CO₂ (eCO₂) and warming are poorly understood. To study this, tomato (Solanum lycopersicum) plants were grown at 400 or 700 ppm CO₂ and 33/28 or 38/33 °C (day/night), and their soil was labeled with ¹⁵NO₃⁻ or ¹⁵NH₄⁺. Plant dry mass, root N-uptake rate, root-to-shoot net N translocation, whole-plant N assimilation, and root resource availability (%C, %N, total nonstructural carbohydrates) were measured. Relative to eCO₂ or warming alone, eCO₂ + warming decreased growth, NO₃⁻ and NH₄⁺-uptake rates, root-to-shoot net N translocation, and whole-plant N assimilation. Decreased N assimilation with eCO₂ + warming was driven mostly by inhibition of NO₃⁻ assimilation, and was not associated with root resource limitations or damage to N-assimilatory proteins. Previously, we showed in tomato that eCO₂ + warming decreases the concentration of N-uptake and -assimilatory proteins in roots, and dramatically increases leaf angle, which decreases whole-plant light capture and, hence, photosynthesis and growth. Thus, decreases in N uptake and assimilation with eCO₂ + warming in tomato are likely due to reduced plant N demand.

The concentration of nitrogen (N) in vegetative tissues is largely dependent on the balance among growth, root N uptake, and N assimilation. Elevated CO₂ (eCO₂) plus warming is likely to affect the vegetative-tissue N and protein concentration of wheat by altering N metabolism, but this is poorly understood. To investigate this, spring wheat (Triticum aestivum) was grown for three weeks at two levels of CO₂ (400 or 700 ppm) and two temperature regimes (26/21 or 31/26 °C, day/night). Plant dry mass, plant %N, protein concentrations, NO₃⁻ and NH₄⁺ root uptake rates (using ¹⁵NO₃ or ¹⁵NH₄), and whole-plant N- and NO₃^--assimilation were measured. Plant growth, %N, protein concentration, and root N-uptake rate were each significantly affected only by CO₂, while N- and NO₃⁻-assimilation were significantly affected only by temperature. However, plants grown at eCO₂ plus warming had the lowest concentrations of N and protein. These results suggest that one strategy breeding programs can implement to minimize the negative effects of eCO₂ and warming on wheat tissue N would be to target the maintenance of root N uptake rate at eCO₂ and N assimilation at higher growth temperatures.

Hydroponic growing systems are advantageous for nutrient studies in which root data are important because they alleviate the laborious and time-consuming task of washing roots to remove soilless substrate particulates from them. However, the growing system should be optimized for the crop of interest. Our overall objective was to develop a protocol for hydroponic strawberry ( Fragaria × ananassa ) production that provided growth equal to or better than soilless substrate. Plants were initially grown in perlite, sand, deep water culture (DWC), or a peat-based soilless substrate. Aboveground plant growth in DWC was similar to that of plants grown in the peat-based substrate and required minimal effort to harvest the entire root system. However, the pH of the DWC nutrient solution decreased to 4.0 ± 0.1 (mean ± SE ) when plants were provided a modified strawberry (Yamazaki) nutrient solution with a ratio of nitrate (NO ₃ ⁻ ) to ammonium (NH ₄ ⁺ ) of 80:20. As a result, a subsequent trial was conducted to evaluate the buffering capacity of nutrient solutions with NO ₃ ⁻ to NH ₄ ⁺ ratios of 0:100, 20:80, 50:50, 60:40, 80:20, or 100:0, with the addition of potassium bicarbonate (KHCO ₃ ). Up to 2.6 mM KHCO ₃ did not provide adequate buffering in nutrient solutions containing NH ₄ ⁺ (0:100 to 80:20 treatments), and nutrient solution pH decreased by ∼1.5 units every 2 to 3 days. The 100% NO ₃ ⁻ nutrient solution, however, maintained a stable pH of 5.9 ± 0.1 when buffered with 0.8 mM KHCO ₃ . Finally, 2(N-Morpholino)ethanesulfonic acid (MES) was evaluated as a potential buffering agent for DWC strawberry production. Plants were grown in a nutrient solution with a 60:40 ratio of NO ₃ ⁻ :NH ₄ ⁺ . The buffering capacity of the nutrient solution increased as the MES concentration supplied increased from 1 to 5 mM, and the 5 mM MES treatment maintained a pH of 5.6 ± 0.2. In summary, strawberry plants can successfully be grown hydroponically in DWC, provided that nutrient solution pH is adequately managed. The addition of MES buffer provided better pH stability than KHCO ₃ .