Background: The agriculturally important pasture grass tall fescue (Festuca arundinaceaSchreb. syn.Lolium arundinaceum(Schreb.) Darbysh.) is an outbreeding allohexaploid, that may be more accurately described as a species complex consisting of three major (Continental, Mediterranean and rhizomatous) morphotypes. Observation of hybrid infertility in some crossing combinations between morphotypes suggests the possibility of independent origins from different diploid progenitors. This study aims to clarify the evolutionary relationships between each tall fescue morphotype through phylogenetic analysis using two low-copy nuclear genes (encoding plastid acetyl-CoA carboxylase [Acc1] andcentroradialis[CEN]), the nuclear ribosomal DNA internal transcribed spacer (rDNA ITS) and the chloroplast DNA (cpDNA) genome-locatedmatKgene. Other taxa within the closely relatedLolium-Festucaspecies complex were also included in the study, to increase understanding of evolutionary processes in a taxonomic group characterised by multiple inter-specific hybridisation events.Results: Putative homoeologous sequences from both nuclear genes were obtained from each polyploid species and compared to counterparts from 15 diploid taxa. Phylogenetic reconstruction confirmedF. pratensisandF. arundinaceavar.glaucescensas probable progenitors to Continental tall fescue, and these species are also likely to be ancestral to the rhizomatous morphotype. However, these two morphotypes are sufficiently distinct to be located in separate clades based on the ITS-derived data set. All four of the generated data sets suggest independent evolution of the Mediterranean and Continental morphotypes, with minimal affinity between cognate sequence haplotypes. No obvious candidate progenitor species for Mediterranean tall fescues were identified, and only two putative sub-genome-specific haplotypes were identified for this morphotype.Conclusions: This study describes the first phylogenetic analysis of theFestucagenus to include representatives of each tall fescue morphotype, and to use low copy nuclear gene-derived sequences to identify putative progenitors of the polyploid species. The demonstration of distinct tall fescue lineages has implications for both taxonomy and molecular breeding strategies, and may facilitate the generation of morphotype and/or sub-genome-specific molecular markers.

The New Zealand economy is export‐driven and heavily reliant on the productivity of the pastoral sector. The transformation of native forest and tussock grassland ecologies to temperate grasslands occurred rapidly with the arrival of Europeans. However, this transplanted ecology required the development and use of plant, microbial, animal and management technologies for successful grassland farming. These have enabled New Zealand pastoral agriculture to compete effectively in international markets, without subsidies. The extensive list of plant‐based and associated microbial‐based adaptations, and the management strategies that have enabled the development of highly productive grasslands are described and reviewed. Credible science is required to inform the debate on the environmental impacts of pasture production to avoid misinformation proliferating. This needs transparent and objective integrity from the science community using funding that seeks no defined or preconceived outcomes. Critically, much of the success of New Zealand pastoral farming has been due to the willingness and ability of farmers to use, adapt, adopt and integrate new ideas and technologies into their farming systems. Historic, current and future challenges, and threats that impact on the productivity and sustainability of pastoral agriculture are described and the means to achieve further technology development to manage these is discussed.

Melilotus albus and Melilotus officinalis are widely used in forage production and herbal medicine due to the biological activity of their coumarins, which have many biological and pharmacological activities, including anti-HIV and anti-tumor effects. To comprehensively evaluate M. albus and M. officinalis coumarin content (Cou), morphological variation, and molecular phylogeny, we examined the Cou, five morphological traits and the molecular characterization based on the trnL-F spacer and internal transcribed spacer (ITS) regions of 93 accessions. Significant (p < 0.05) variation was observed in the Cou and all five morphological traits in both species. Analysis of population differentiation (Pst) of the phenotypic traits showed that powdery mildew resistance (PMR) had the greatest Pst, meaning that this trait demonstrated the largest genetic differentiation among the accessions. The Pst values of dry matter yield (DMY) and Cou were relatively high. Biplot analysis identified accessions with higher DMY and higher and lower Cou. Analysis of molecular sequence variation identified seven haplotypes of the trnL-F spacer and 13 haplotypes of the ITS region. Based on haplotype and sequence analyses, the genetic variation of M. officinalis was higher than that of M. albus. Additionally, ITS sequence analysis showed that the variation among accessions was larger than that among species across three geographical areas: Asia, Europe, and North America. Similarly, variation among accessions for both the trnL-F and ITS sequences were larger than the differences between the geographical areas. Our results indicate that there has been considerable gene flow between the two Melilotus species. Our characterization of Cou and the morphological and genetic variations of these two Melilotus species may provide useful insights into germplasm improvement to enhance DMY and Cou.

Epichloë fungal endophytes that systemically colonise cool‐season grasses have become an internationally well‐researched symbiosis. While sexual species of Epichloë can cause choke disease of grass seed heads, asexual forms are symptomless endophytes that colonise embryos to become seed transmitted. In mutualistic associations, the grass benefits through greater tolerance of abiotic and biotic stresses. In New Zealand, at least 12 species of naturalised and native grasses have been documented as infected with a range of asexual or sexual Epichloë . The impact of endophyte‐infected ryegrass and tall fescue has been significant for pastoral farmers, with Epichloë enhancing pasture performance. Production of bioactive secondary metabolites reduces herbivory by invertebrate pests but some are also toxic to grazing animals. Selected strains of Epichloë have been utilised to reduce livestock problems while retaining improved pasture performance. Documented Epichloë ‐infected native grasses are rare, justifying greater efforts of discovery, description and conservation as taonga (a treasure) for Māori and all New Zealanders. Given their ecological success in New Zealand ecosystems, along with economic and environmental benefits, there are good prospects to further develop both endemic and naturalised Epichloë ‐grass associations that may benefit other cool‐season grasses and potentially deliver new beneficial properties.

In-field measurement of yield and growth rate in pasture species is imprecise and costly, limiting scientific and commercial application. Our study proposed a LiDAR-based mobile platform for non-invasive vegetative biomass and growth rate estimation in perennial ryegrass (Lolium perenne L.). This included design and build of the platform, development of an algorithm for volumetric estimation, and field validation of the system. The LiDAR-based volumetric estimates were compared against fresh weight and dry weight data across different ages of plants, seasons, stages of regrowth, sites, and row configurations. The project had three phases, the last one comprising four experiments. Phase 1: a LiDAR-based, field-ready prototype mobile platform for perennial ryegrassrecognition in single row plots was developed. Phase 2: real-time volumetric data capture, modelling and analysis software were developed and integrated and the resultant algorithm was validated in the field. Phase 3. LiDAR Volume data were collected via the LiDAR platform and field-validated in four experiments. Expt.1: single-row plots of cultivars and experimental diploid breeding populations were scanned in the southern hemisphere spring for biomass estimation. Significant (P < 0.001) correlations were observed between LiDAR Volume and both fresh and dry weight data from 360 individual plots (R2 = 0.89 and 0.86 respectively). Expt 2: recurrent scanning of single row plots over long time intervals of a few weeks was conducted, and growth was estimated over an 83 day period. Expt 3: recurrent scanning of single-row plots over nine short time intervals of 2 to 5 days was conducted, and growth rate was observed over a 26 day period. Expt 4: recurrent scanning of paired-row plots over an annual cycle of repeated growth and defoliation was conducted, showing an overall mean correlation of LiDAR Volume and fresh weight of R2 = 0.79 for 1008 observations made across seven different harvests between March and December 2018. Here we report development and validation of LiDAR-based volumetric estimation as an efficient and effective tool for measuring fresh weight, dry weight and growth rate in single and paired-row plots of perennial ryegrass for the first time, with a consistently high level of accuracy. This development offers precise, non-destructive and cost-effective estimation of these economic traits in the field for ryegrass and potentially other pasture grasses in the future, based on the platform and algorithm developed for ryegrass.