Unmanned aerial vehicle (UAV)-based remote sensing (RS) possesses the significant advantage of being able to efficiently collect images for precision agricultural applications. Although numerous methods have been proposed to monitor crop nitrogen (N) status in recent decades, just how to utilize an appropriate modeling algorithm to estimate crop leaf N content (LNC) remains poorly understood, especially based on UAV multispectral imagery. A comparative assessment of different modeling algorithms (i.e., simple and non-parametric modeling algorithms alongside the physical model retrieval method) for winter wheat LNC estimation is presented in this study. Experiments were conducted over two consecutive years and involved different winter wheat varieties, N rates, and planting densities. A five-band multispectral camera (i.e., 490 nm, 550 nm, 671 nm, 700 nm, and 800 nm) was mounted on a UAV to acquire canopy images across five critical growth stages. The results of this study showed that the best-performing vegetation index (VI) was the modified renormalized difference VI (RDVI), which had a determination coefficient (R²) of 0.73 and a root mean square error (RMSE) of 0.38. This method was also characterized by a high processing speed (0.03 s) for model calibration and validation. Among the 13 non-parametric modeling algorithms evaluated here, the random forest (RF) approach performed best, characterized by R² and RMSE values of 0.79 and 0.33, respectively. This method also had the advantage of full optical spectrum utilization and enabled flexible, non-linear fitting with a fast processing speed (2.3 s). Compared to the other two methods assessed here, the use of a look up table (LUT)-based radiative transfer model (RTM) remained challenging with regard to LNC estimation because of low prediction accuracy (i.e., an R² value of 0.62 and an RMSE value of 0.46) and slow processing speed. The RF approach is a fast and accurate technique for N estimation based on UAV multispectral imagery.

High-resolution satellite images can be used to some extent to mitigate the mixed-pixel problem caused by the lack of intensive production, farmland fragmentation, and the uneven growth of field crops in developing countries. Specifically, red-edge (RE) satellite images can be used in this context to reduce the influence of soil background at early stages as well as saturation due to crop leaf area index (LAI) at later stages. However, the availability of high-resolution RE satellite image products for research and application globally remains limited. This study uses the weight-and-unmixing algorithm as well as the SUPer-REsolution for multi-spectral Multi-resolution Estimation (Wu-SupReME) approach to combine the advantages of Sentinel-2 spectral and Planet spatial resolution and generate a high-resolution RE product. The resultant fused image is highly correlated (R² > 0.98) with Sentinel-2 image and clearly illustrates the persistent advantages of such products. This fused image was significantly more accurate than the originals when used to predict heterogeneous wheat LAI and therefore clearly illustrated the persistence of Sentinel-2 spectral and Planet spatial advantage, which indirectly proved that the fusion methodology of generating high-resolution red-edge products from Planet and Sentinel-2 images is possible. This study provided method reference for multi-source data fusion and image product for accurate parameter inversion in quantitative remote sensing of vegetation.

Unmanned aerial vehicles (UAVs) equipped with dual-band crop-growth sensors can achieve high-throughput acquisition of crop-growth information. However, the downwash airflow field of the UAV disturbs the crop canopy during sensor measurements. To resolve this issue, we used computational fluid dynamics (CFD), numerical simulation, and three-dimensional airflow field testers to study the UAV-borne multispectral-sensor method for monitoring crop growth. The results show that when the flying height of the UAV is 1 m from the crop canopy, the generated airflow field on the surface of the crop canopy is elliptical, with a long semiaxis length of about 0.45 m and a short semiaxis of about 0.4 m. The flow-field distribution results, combined with the sensor’s field of view, indicated that the support length of the UAV-borne multispectral sensor should be 0.6 m. Wheat test results showed that the ratio vegetation index (RVI) output of the UAV-borne spectral sensor had a linear fit coefficient of determination (R²) of 0.81, and a root mean square error (RMSE) of 0.38 compared with the ASD Fieldspec2 spectrometer. Our method improves the accuracy and stability of measurement results of the UAV-borne dual-band crop-growth sensor. Rice test results showed that the RVI value measured by the UAV-borne multispectral sensor had good linearity with leaf nitrogen accumulation (LNA), leaf area index (LAI), and leaf dry weight (LDW); R² was 0.62, 0.76, and 0.60, and RMSE was 2.28, 1.03, and 10.73, respectively. Our monitoring method could be well-applied to UAV-borne dual-band crop growth sensors.

The impact of global warming on crop growth periods and yields has been evaluated by using crop models, which need to provide various kinds of input datasets and estimate numerous parameters before simulation. Direct studies on the changes of climatic factors on the observed crop growth and yield could provide a more simple and intuitive way for assessing the impact of climate change on crop production. In this study, four cultivars which were planted over more than 15 years in eight test stations in the Northern Winter Wheat Region of China were selected to investigate the relationships between growth periods, grain yields, yield components and temperatures. It was found that average temperatures and heat degree-days (HDD) during the winter wheat growing seasons tended to increase over time series at most study sites. The length of growth period and growing degree days (GDD) were not fixed for a given cultivar among different years and locations, and the variation on the periods from sowing to jointing was relatively greater than in the other periods. The increasing temperature mainly shortened the periods from sowing to jointing and jointing to anthesis, which led to the decrease in entire growth periods. Positive relationships between spike number, grain number per spike, grain yields and average temperatures were identified in the Northern Winter Wheat Region of China. The grain yield in the study area increased by 406.3 kg ha⁻¹ for each 1 °C increase in average temperature. Further, although the positive relationship between grain yield and HDD was found in our study, the heat stress did not lead to the wheat yield decline in the study region. Temperature is a major determinant of wheat growth and development, the average temperature and the frequency of heat stresses are projected to increase in the future, so understanding the effect of temperature on wheat production and adopting appropriate adaptation are required for the implementation of food security policies.

Global warming can have detrimental effects on crop production formation, but the effect of low-temperature stress on crop quality should not be ignored. Wheat is one of the main grain crops in the world, and the quality of wheat is directly related to human health. The nutritional importance of wheat in the human diet necessitates pursuing a study to collect detailed and accurate knowledge of the amino acid composition of wheat proteins under low-temperature conditions. To better understand the effect of low temperature on the composition of amino acids in mature wheat, we conducted a two-year low-temperature-controlled wheat pot experiment in artificial climate chambers with two different wheat cultivars at four low-temperature intensities during the jointing, booting, and both jointing and booting stages. Our results suggest that the contents of total amino acids, essential amino acids, and nonessential amino acids for the cold-sensitive wheat cultivar (Yangmai16) increased under the low-temperature treatments, while the contents of the cold-tolerant cultivar (Xumai30) decreased when low-temperature was applied during the jointing and double (both jointing and booting) stages. Through the amino acid score (AAS), we found that the first limiting amino acid was Lys, while the second limiting amino acid varied among Ile, Met + Cys, and Val after the low-temperature treatments. Comparing the amino acid ratio coefficients (RCs), we found that Leu and Thr in wheat grains were close to the standard protein after the low-temperature treatments, while Phe and Tyr were in a surplus, and the other essential amino acids did not meet the standard. Thus, to improve the protein quality of wheat, protective measures should be taken when low temperatures occur.