The lower reaches of the Yellow River is known for the rapid development of industry and agriculture, which has also led to some pollution. However, information about the level of toxic contaminants in the surface waters is lacking in this area. Therefore, five sampling points were set in the lower Yellow River to investigate the distribution of various pollutants and analyze the potential risks. The presence of heavy metals (Heavy metals tested for in this study were: Mercury (Hg), Arsenic (As), Copper (Cu), Chromium (Cr), and Zinc (Zn)) and antibiotics (Antibiotics tested for in this study were: Enrofloxacin (ENR), Ciprofloxacin (CIP), and Norfloxacin (NOR)) in water samples taken from the lower Yellow River were measured to reveal the spatial distribution and risk potential of the compounds. Various water quality parameters (Water quality parameters used in this study were: chemical oxygen demand (COD), biological oxygen demand (BOD₅), total phosphorus (TP), and total nitrogen (TN)) were also tested. Study results showed the main surface water pollution components were COD, BOD₅, TN, and TP. The average levels were 37.79 mg/L, 16.64 mg/L, 4.14 mg/L, and 0.42 mg/L, respectively. Among the detected metals from the water samples, Hg (LOD-0.1 μg/L) levels were only in line with the surface water class III or worse. Both fish and water samples contained antibiotics. According to an ecological risk assessment conducted along the river, the distribution of pollutants in the waters exhibited a spatial relationship with the land-use pattern in the study region and the Kenli site was the most polluted. Research shows that up-to-date data on the residual levels and distribution characteristics of pollutants in the lower Yellow River could provide valuable baseline data and technical support for relevant government departments and their management going forward.

To expand germ cell populations and provide a consistent supply for transplantation, we established basal culture conditions for sturgeon germ cells and subsequently increased their mitotic activity by eliminating gonad somatic cells, supplementing with growth factor, and replacing fetal bovine serum (FBS). The initial basal culture conditions were Leibovitz’s L-15 medium (pH 8.0) supplemented with 5% FBS (p < 0.001) at 21 °C. Proliferation of germ cells was significantly enhanced and maintained for longer periods by elimination of gonad somatic cells and culture under feeder-cell free conditions, with addition of leukemia inhibitory factor and glial-cell-derived neurotrophic factor (p < 0.001). A serum-free culture medium improved germ cell proliferation compared to the L-15 with FBS (p < 0.05). Morphology remained similar to that of fresh germ cells for at least 40 d culture. Germline-specific gene expression analysis revealed no significant changes to germ cells before and after culture. Sterlet Acipenser ruthenus germ cells cultured more than 40 days showed development after transplant into Russian sturgeon Acipenser gueldenstaedtii. Polymerase chain reaction showed 33.3% of recipient gonads to contain sterlet cells after four months. This study developed optimal culture condition for sturgeon germ cells. Germ cells after 40 d culture developed in recipient gonads. This study provided useful information for culture of sturgeon germ cells.