Significance: Ambient humidity can influence the survival of pathogens in respiratory aerosols and droplets, although the mechanism and optimum humidity level for public health remain unclear. Here, we present evidence for a humidity-dependent, semisolid state of aerosols and droplets relevant to pathogen survival. These observations indicate that a semisolid state may protect pathogens from inactivation by hindering disinfection reactions at intermediate-to-low humidity levels. The formation of the semisolid state was dependent on the composition of the aerosols, which suggests that the humidity for optimum pathogen destruction will depend on the composition of respiratory particles released from an infected host. These observations can be used to help interpret laboratory studies and inform public health recommendations.

Levitation experiments reveal molecular interactions that can alter the expected properties of atmospheric aerosol.

Inadequate knowledge of the phase state of atmospheric particles represents a source of uncertainty in global climate and air quality models. Hygroscopic aqueous inorganic particles are often assumed to remain liquid throughout their atmospheric lifetime or only (re)crystallize at low relative humidity (RH) due to the kinetic limitations of efflorescence (salt crystal nucleation and growth from an aqueous solution). Here we present experimental observations of a previously unexplored heterogeneous nucleation pathway that we have termed “contact efflorescence,” which describes efflorescence initiated by an externally located solid particle coming into contact with the surface of a metastable aqueous microdroplet. This study demonstrates that upon a single collision, contact efflorescence is a pathway for crystallization of atmospherically relevant aqueous particles at high ambient RH (≤80%). Soluble inorganic crystalline particles were used as contact nuclei to induce efflorescence of aqueous ammonium sulfate [(NH₄)₂SO₄], sodium chloride (NaCl), and ammonium nitrate (NH₄NO₃), with efflorescence being observed in several cases close to their deliquescence RH values (80%, 75%, and 62%, respectively). To our knowledge, these observations represent the highest reported efflorescence RH values for microdroplets of these salts. These results are particularly important for considering the phase state of NH₄NO₃, where the contact efflorescence RH (∼20–60%) is in stark contrast to the observation that NH₄NO₃ microdroplets do not homogeneously effloresce, even when exposed to extremely arid conditions (<1% RH). Considering the occurrence of particle collisions in the atmosphere (i.e., coagulation), these observations of contact efflorescence challenge many assumptions made about the phase state of inorganic aerosol.