Magnetic fields are an unavoidable physical factor affecting living organisms. Lettuce seeds (Lactuca sativa var. cabitat L.) were subjected to various intensities of the static magnetic field (SMF) viz., MF0 (control), SMF1 (0.44 Tesla (T), SMF2 (0.77 T), and SMF3 (1 T) for three exposure times (1, 2, and 3 h). SMF-treated seedlings showed induction in growth parameters and metabolism comparing to control. All photosynthetic pigments were induced markedly under SMF, especially chlorophyll a. SMF at different intensities boosted osmolytes, non-enzymatic antioxidants, and the phenylalanine ammonia-lyase activity over non-magnetized seedlings. Oxidative damage criteria viz., hydrogen peroxide, superoxide radical, and lipid peroxidation, as well as polyphenol oxidase activity, were kept at low values under SMF-treated seeds relative to control, especially SMF2. Electron donors to antioxidant enzymes including nitrate reductase, nitric oxide, and hydrogen sulfide induced via SMF exposure and consequently the activities of superoxide dismutase, glutathione-S-transferases, catalase, and peroxidases family enzymes were also stimulated under SMF, whatever the intensity or the exposure period applied. All these regulations reflected on the enhancement of lettuce yield production which reached 50% over the control at SMF3. Our findings offered that SMF-seed priming is an innovative and low-cost strategy that can improve the growth, bioactive constituents, and yield of lettuce.

Application of nutrients as nanoparticle (NP) is an operative manner of nutrient supply for plants, especially under stress conditions. The present study was designed to highlight the role of iron NP on drought tolerance and elucidate the underlying mechanisms in drought-stressed canola plants. Drought stress was imposed by polyethylene glycol different concentrations (0, 10 and 15% (W/V)) with or without iron NP (1.5 and 3 mg/l). A comparative study of several physiological and biochemical parameters have been carried out in canola plants treated by drought and iron NP. Stressed-canola plants showed a reduction in growth parameters, whereas iron NP mostly stimulated growth of stressed plants, which was accompanied by reinforcement in defense mechanisms. Regarding impacts on compatible osmolytes, the data revealed that iron NP was able to regulate osmotic potential by increasing protein, proline and soluble sugar contents. The iron NP application was activated the enzymatic defense system (catalase and polyphenol oxidase) and promoted the non-enzymatic antioxidants (phenol, flavonol and flavonoid). Both of these adaptive responses declined free radicals as well as lipid peroxidation and enhanced the membrane stability and drought tolerance of the plants. Enhanced chlorophyll accumulation via induction of protoporphyrin, magnesium protoporphyrin and protochlorophyllide, by iron NP also contributed towards better stress tolerance. Enzymes of Krebs cycle, namely succinate dehydrogenase and aconitase, were induced by iron NP in canola plants grown under drought stress. These results propose a multifaceted involvement of iron NP, through regulation of activity of respiratory enzymes and antioxidant enzymes, production of reactive oxygen species, osmoregulation and secondary metabolites metabolism, in response to drought stress.

This work aimed to study the impact of exogenously applied selenium nanoparticle (Se NP) on physiological and biochemical attributes of canola to improve the drought tolerance. Drought stress was imposed by poly ethylene glycol at different concentrations (0, 10 and 15% (W/V)) with or without Se NP (2 and 5 mg/L). Se NP increased plant growth in a dose-dependent manner during drought stress. Se NP boosted chlorophyll precursors such as protoporphyrin, magnesium protoporphyrin, thereby inducing chlorophyll biosynthesis. Enzymes of Krebs cycle, namely aconitase and succinate dehydrogenase, up-regulated by Se NP in canola plants subjected to drought stress. Se NP aided to osmotic regulation and osmotic protection by accumulating osmotic regulatory substances (proline, soluble sugar and protein). Treatment with Se NP reinforced antioxidant defense mechanisms by inducing superoxide dismutase, catalase, peroxidase, total phenol and tocopherol, thereby reducing hydrogen peroxide and malondialdehyde as oxidative stress markers. Thus, Se NP facilitated free radicals scavenging in stressed plants. Generally, Se NP acts as activator for drought stress-related resistance responses and causes the improvement of canola tolerance under drought conditions.