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Penicillium expansum is an important postharvest pathogen of pomaceous fruit and a causal agent of blue mold or soft rot. In this study, we investigated the effect of ambient pH on growth, ultrastructure alteration, and pathogenicity of P. expansum, as well as accumulation of patulin and expression of genes involved in patulin biosynthesis. Under different pH, the fungus was routinely cultured and collected for growth, pathogenicity, patulin production, and gene expression studies using transmission electron microscopy, apple inoculation, HPLC, and RT-qPCR methods. Different ambient pH had significant impact on expression of genes and growth factors involved in patulin biosynthesis. Under same range of pH, gene expression profile, growth factors, and patulin accumulation (in vivo and in vitro) all showed similar changing trends. A well-developed cell was observed in addition to upregulation of genes at pH between pH 5.0 and 7.0, while the opposite was observed when pH was too basic (8.5) or too acid (2.5). Additionally, ambient pH had direct or indirect influence on expression of PecreaA, PelaeA, and PepacC. These findings will help in understanding the effect of ambient pH on growth, pathogenicity, and patulin production and support the development of successful methods for combating P. expansum infection on apple fruits.
Carelle Jimdjio; Huali Xue; Yang Bi; Mina Nan; Lan Li; Rui Zhang; Qili Liu; Lumei Pu. Effect of Ambient pH on Growth, Pathogenicity, and Patulin Production of Penicillium expansum. Toxins 2021, 13, 550 .
AMA StyleCarelle Jimdjio, Huali Xue, Yang Bi, Mina Nan, Lan Li, Rui Zhang, Qili Liu, Lumei Pu. Effect of Ambient pH on Growth, Pathogenicity, and Patulin Production of Penicillium expansum. Toxins. 2021; 13 (8):550.
Chicago/Turabian StyleCarelle Jimdjio; Huali Xue; Yang Bi; Mina Nan; Lan Li; Rui Zhang; Qili Liu; Lumei Pu. 2021. "Effect of Ambient pH on Growth, Pathogenicity, and Patulin Production of Penicillium expansum." Toxins 13, no. 8: 550.
Benzo-(1, 2, 3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) is the first synthetic chemical elicitor that induces resistance against postharvest diseases in fruit and vegetables. However, little information is available on how BTH application affects membrane lipid metabolism and volatile compounds (VOCs) biosynthesis in infected fruit. The results of this study indicated that BTH treatment maintained cell membrane integrity, increased phospholipase A2 (PLA2) activity, and decreased phospholipase C (PLC) and D (PLD) activities in Penicillium expansum-inoculated apple fruit. BTH enhanced phosphatidic acid (PA) content, maintained phosphatidylcholine (PC) and phosphatidylinositol (PI) levels, and increased unsaturated fatty acids (USFA) content. Additionally, BTH decreased the activity of lipoxygenase (LOX), hydroperoxidelyase (HPL), alcohol dehydrogenase (ADH) and alcohol acyltransferase (AAT), and reduced the content of straight-chain VOCs in inoculated fruit. Therefore, BTH could maintain cell membrane integrity in the P. expansum-inoculated apple fruit by inhibiting PLD and PLC activities and PA content. A higher accumulation of USFAs is contributed by increasing PLA2 activity with BTH. In addition, BTH could inhibit the enzymes activities of HPL, LOX, ADH and AAT in LOX pathway, resulting in a less release of straight-chain VOCs in the inoculated fruit during incubation.
Di Gong; Yang Bi; Xuemei Zhang; Zhanhong Han; Yuanyuan Zong; Yongcai Li; Edward Sionov; Dov Prusky. Benzothiadiazole treatment inhibits membrane lipid metabolism and straight-chain volatile compound release in Penicillium expansum-inoculated apple fruit. Postharvest Biology and Technology 2021, 181, 111671 .
AMA StyleDi Gong, Yang Bi, Xuemei Zhang, Zhanhong Han, Yuanyuan Zong, Yongcai Li, Edward Sionov, Dov Prusky. Benzothiadiazole treatment inhibits membrane lipid metabolism and straight-chain volatile compound release in Penicillium expansum-inoculated apple fruit. Postharvest Biology and Technology. 2021; 181 ():111671.
Chicago/Turabian StyleDi Gong; Yang Bi; Xuemei Zhang; Zhanhong Han; Yuanyuan Zong; Yongcai Li; Edward Sionov; Dov Prusky. 2021. "Benzothiadiazole treatment inhibits membrane lipid metabolism and straight-chain volatile compound release in Penicillium expansum-inoculated apple fruit." Postharvest Biology and Technology 181, no. : 111671.
The dry rot of potato caused by Fusarium sulphureum is the main postharvest disease that causes serious economic loss and leads to mycotoxin contamination. Therefore, it is necessary to find a strategy to increase the plant’s resistance against disease. T-2 toxin at low concentration can act as an elicitor to induce resistance against disease. Calcium ion plays a vital role in induced resistance by responding the environmental stress in plant. In this study, the effect of Ca2+ on the development of dry rot, weight loss rate, the suberin polyphenolic (SPP) and suberin polyaliphatic (SPA) accumulation and their participation in phenylpropanoid metabolism were investigated in treated potato. The results showed that lesion diameter and weight loss rate significantly increased after ethylenebis (oxyethylenenitrilo) tetraacetic acid (EGTA) treatment, but the addition of exogenous Ca2+ noticeably inhibited the increase. The accumulations of SPP and SPA, enzymatic activities, gene expressions, as well as the contents of total phenols and lignin and their major substrates involved in phenylpropanoid metabolism were decreased after EGTA treatment, these indicators were recovered after the addition of exogenous Ca2+. Therefore, the results suggested that Ca2+ treatment induced resistance against dry rot, inhibited weight loss rate and accelerated the accumulation of SPP and SPA by activating phenylpropanoid metabolism in treated potato.
Rui Zhang; Lan Li; Huali Xue; Yang Bi; Hussain Raza; Min Si; Hui Peng; Mina Nan; Yuanyuan Zong; Dov Prusky. Ca2+ applications affect the phenylpropanoid metabolism in potato tubers induced by T-2 toxin. Postharvest Biology and Technology 2021, 180, 111616 .
AMA StyleRui Zhang, Lan Li, Huali Xue, Yang Bi, Hussain Raza, Min Si, Hui Peng, Mina Nan, Yuanyuan Zong, Dov Prusky. Ca2+ applications affect the phenylpropanoid metabolism in potato tubers induced by T-2 toxin. Postharvest Biology and Technology. 2021; 180 ():111616.
Chicago/Turabian StyleRui Zhang; Lan Li; Huali Xue; Yang Bi; Hussain Raza; Min Si; Hui Peng; Mina Nan; Yuanyuan Zong; Dov Prusky. 2021. "Ca2+ applications affect the phenylpropanoid metabolism in potato tubers induced by T-2 toxin." Postharvest Biology and Technology 180, no. : 111616.
Candida oleophila is a type of biocontrol yeast offering effective postharvest disease control. To the best of our knowledge, the effect of C. oleophila upon the healing of tubers is yet to be studied. The present study addresses the existing knowledge gap by investigating the effect of C. oleophila on wound healing in potato tubers. The results show that C. oleophila colonized and proliferated at the wound sites during the early and intermediate stages of healing. In addition, C. oleophila reduced weight loss of wounded tubers, decreased disease index of inoculated tubers with Fusarium sulphureum, and accelerated accumulation of suberin poly phenolic (SPP) and lignin at wound sites. C. oleophila activated phenylpropanoid metabolism and increased the content of SPP monomers, total phenol, flavonoids, and lignin. Furthermore, the yeast increased H2O2 content as well as peroxidase activity.
Xiaoyuan Zheng; Hong Jiang; Esrat Silvy; Shijia Zhao; Xiuwei Chai; Bin Wang; Zhicheng Li; Yang Bi; Dov Prusky. Candida Oleophila Proliferated and Accelerated Accumulation of Suberin Poly Phenolic and Lignin at Wound Sites of Potato Tubers. Foods 2021, 10, 1286 .
AMA StyleXiaoyuan Zheng, Hong Jiang, Esrat Silvy, Shijia Zhao, Xiuwei Chai, Bin Wang, Zhicheng Li, Yang Bi, Dov Prusky. Candida Oleophila Proliferated and Accelerated Accumulation of Suberin Poly Phenolic and Lignin at Wound Sites of Potato Tubers. Foods. 2021; 10 (6):1286.
Chicago/Turabian StyleXiaoyuan Zheng; Hong Jiang; Esrat Silvy; Shijia Zhao; Xiuwei Chai; Bin Wang; Zhicheng Li; Yang Bi; Dov Prusky. 2021. "Candida Oleophila Proliferated and Accelerated Accumulation of Suberin Poly Phenolic and Lignin at Wound Sites of Potato Tubers." Foods 10, no. 6: 1286.
Lignin is an important component of the healing tissue in fruits. In this study, we treated muskmelon (Cucumis melo L. cv. “Manao”) fruit with exogenous nitric oxide (NO) donor sodium nitroprusside (SNP) to observe and analyze its effect on lignin synthesis and accumulation during healing. Results showed that SNP treatment enhanced the contents of endogenous NO and H2O2, increased the activities of phenylalanine ammonia lyase, cinnamate 4 hydroxylase, cinnamyl alcohol dehydrogenase, and peroxidase, and raised the contents of sinapyl alcohol, coniferyl alcohol, coumaryl alcohol, and lignin. SNP augmented the hardness of the healing tissue and decreased its resilience, springiness, and cohesiveness. In addition, SNP treatment effectively reduced the weight loss and disease index of wounded muskmelons. All these results suggest that lignin metabolism mediated by NO play a crucial role in wound healing of muskmelons.
Bin Wang; Zhicheng Li; Zhanhong Han; Sulin Xue; Yang Bi; Dov Prusky. Effects of nitric oxide treatment on lignin biosynthesis and texture properties at wound sites of muskmelons. Food Chemistry 2021, 362, 130193 .
AMA StyleBin Wang, Zhicheng Li, Zhanhong Han, Sulin Xue, Yang Bi, Dov Prusky. Effects of nitric oxide treatment on lignin biosynthesis and texture properties at wound sites of muskmelons. Food Chemistry. 2021; 362 ():130193.
Chicago/Turabian StyleBin Wang; Zhicheng Li; Zhanhong Han; Sulin Xue; Yang Bi; Dov Prusky. 2021. "Effects of nitric oxide treatment on lignin biosynthesis and texture properties at wound sites of muskmelons." Food Chemistry 362, no. : 130193.
β-Aminobutyric acid (BABA) is a nonprotein amino acid that induces plant defense responses to numerous biotic and abiotic stresses. However, whether BABA treatment affects the wound healing process of potato tubers has yet to be reported. In this study, we examined the effects of BABA treatment on the weight loss and disease index of Solanum tuberosum L. cv. Atlantic potato tubers inoculated with Fusarium sulphureum and assessed the deposition of suberin polyphenolic (SPP) and lignin at the wound sites of these tubers during healing. We also investigated the indexes involved in phenylpropane metabolism, H2O2, and peroxidase in the underlying effect mechanism. BABA treatment effectively reduced the weight loss of wounded tubers (by 41.0 %) and the disease index of inoculated tubers (by 43.2 %) during healing (at 21 d and relative to the control). BABA treatment also accelerated the deposition of SPP and lignin at wound sites. SPP and lignin cell layers in the treated tubers were 58.45 % and 54.72 % thicker, respectively, than the equivalent cell layers in the controls at 7 d of healing. Additionally, BABA treatment significantly enhanced the activities of phenylalanine ammonia-lyase (PAL), 4-coumaryl coenzyme A ligase (4CL), cinnamic acid-4-hydroxylase (C4H), and cinnamyl alcohol dehydrogenase (CAD) while also promoting the synthesis of cinnamic acid, p-coumaric acid, caffeic acid, ferulic acid, sinapic acid, cinnamyl alcohol, coniferyl alcohol, and sinapyl alcohol, as well as increasing total phenolic and lignin content, at wound sites during healing. Furthermore, BABA treatment increased H2O2 content and peroxidase activity at the wounds. These results suggest that BABA treatment activates phenylpropanoid metabolism, increases H2O2 content and peroxidase activity, and accelerates the deposition of SPP and lignin in potato tuber wounds, all of which lead to reductions in the weight loss and disease index of tubers during healing.
Yatong Zhu; Yuanyuan Zong; Wei Liang; Ackah Sabina; Xiuwei Chai; Yongcai Li; Yang Bi; Prusky Dov. β-Aminobutyric acid treatment accelerates the deposition of suberin polyphenolic and lignin at wound sites of potato tubers during healing. Postharvest Biology and Technology 2021, 179, 111566 .
AMA StyleYatong Zhu, Yuanyuan Zong, Wei Liang, Ackah Sabina, Xiuwei Chai, Yongcai Li, Yang Bi, Prusky Dov. β-Aminobutyric acid treatment accelerates the deposition of suberin polyphenolic and lignin at wound sites of potato tubers during healing. Postharvest Biology and Technology. 2021; 179 ():111566.
Chicago/Turabian StyleYatong Zhu; Yuanyuan Zong; Wei Liang; Ackah Sabina; Xiuwei Chai; Yongcai Li; Yang Bi; Prusky Dov. 2021. "β-Aminobutyric acid treatment accelerates the deposition of suberin polyphenolic and lignin at wound sites of potato tubers during healing." Postharvest Biology and Technology 179, no. : 111566.
Suberin poly phenolic is an important component of healing tissue. We aimed to develop a new method to accelerate wound healing of harvested muskmelons. In this study, the plants and fruits of muskmelons were sprayed with 0.1 % chitosan four times during fruit development to evaluate the effect of chitosan treatment on wound healing of harvested muskmelons. The results showed that preharvest chitosan sprays reduced the weight loss and disease index of harvested muskmelons during healing. Chitosan sprays also accelerated the deposition of suberin poly phenolic at wound sites of harvested muskmelons during healing. The treatment increased the activities of phenylalanine ammonia-lyase and cinnamate-4-hydroxylase, and promoted the accumulation of cinnamic acid, caffeic acid, ferulic acid, p-coumaric acid, total phenols and flavonoids at wound sites. We suggest that preharvest chitosan sprays activated phenylpropanoid metabolism, promoted the deposition of suberin poly phenolic at wound sites of harvested muskmelons.
Zhicheng Li; Sulin Xue; Xiaoqin Xu; Bin Wang; Xiaoyuan Zheng; Baojun Li; Pengdong Xie; Yang Bi; Dov Prusky. Preharvest multiple sprays with chitosan accelerate the deposition of suberin poly phenolic at wound sites of harvested muskmelons. Postharvest Biology and Technology 2021, 179, 111565 .
AMA StyleZhicheng Li, Sulin Xue, Xiaoqin Xu, Bin Wang, Xiaoyuan Zheng, Baojun Li, Pengdong Xie, Yang Bi, Dov Prusky. Preharvest multiple sprays with chitosan accelerate the deposition of suberin poly phenolic at wound sites of harvested muskmelons. Postharvest Biology and Technology. 2021; 179 ():111565.
Chicago/Turabian StyleZhicheng Li; Sulin Xue; Xiaoqin Xu; Bin Wang; Xiaoyuan Zheng; Baojun Li; Pengdong Xie; Yang Bi; Dov Prusky. 2021. "Preharvest multiple sprays with chitosan accelerate the deposition of suberin poly phenolic at wound sites of harvested muskmelons." Postharvest Biology and Technology 179, no. : 111565.
Trichothecium roseum is an important postharvest pathogen, belonging to an alkalizing group of pathogens secreting ammonia during fungal growth and colonization of apple fruits. Fungal pH modulation is usually considered a factor for improving fungal gene expression, contributing to its pathogenicity. However, the effects of inoculation with T. roseum spore suspensions at increasing pH levels from pH 3 up to pH 7, on the reactive oxygen species (ROS) production and scavenging capability of the apple fruits, affecting host susceptibility, indicate that the pH regulation by the pathogens also affects host response and may contribute to colonization. The present results indicate that the inoculation of T. roseum spores at pH 3 caused the lowest cell membrane permeability, and reduced malondialdehyde content, NADPH oxidases activity, O2 ●− and H2O2 production in the colonized fruit. Observations of the colonized area on the 9th day after inoculation at pH 3, showed that the rate of O2 ●− production and H2O2 content was reduced by 57% and 25%, compared to their activities at pH 7. In contrast, antioxidative activities of superoxide dismutase, catalase and peroxidases of fruit tissue inoculated with spores’ suspension in the presence of a solution at pH 3.0 showed their highest activity. The catalase and peroxidases activities in the colonized tissue at pH 3 were higher by almost 58% and 55.9%, respectively, on the 6th day after inoculation compared to inoculation at pH 7. The activities of key enzymes of the ascorbate-glutathione (AsA-GSH) cycle and their substrates and products by the 9th day after fruit inoculation at pH 3 showed 150%, 31%, 16%, and 110% higher activities of ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase and glutathione reductase, respectively, compared to pH 7. A similar pattern of response was also observed in the accumulation of ascorbic acid and dehydroascorbate which showed a higher accumulation at pH 3 compared to the colonization at pH 7. The present results indicate that the metabolic regulation of the pH environment by the T. roseum not only modulates the fungal pathogenicity factors reported before, but it induces metabolic host changes contributing both together to fungal colonization.
Zhanhong Han; Zhenyu Wang; Yang Bi; Yuanyuan Zong; Di Gong; Bin Wang; Baojun Li; Edward Sionov; Dov Prusky. The Effect of Environmental pH during Trichothecium roseum (Pers.:Fr.) Link Inoculation of Apple Fruits on the Host Differential Reactive Oxygen Species Metabolism. Antioxidants 2021, 10, 692 .
AMA StyleZhanhong Han, Zhenyu Wang, Yang Bi, Yuanyuan Zong, Di Gong, Bin Wang, Baojun Li, Edward Sionov, Dov Prusky. The Effect of Environmental pH during Trichothecium roseum (Pers.:Fr.) Link Inoculation of Apple Fruits on the Host Differential Reactive Oxygen Species Metabolism. Antioxidants. 2021; 10 (5):692.
Chicago/Turabian StyleZhanhong Han; Zhenyu Wang; Yang Bi; Yuanyuan Zong; Di Gong; Bin Wang; Baojun Li; Edward Sionov; Dov Prusky. 2021. "The Effect of Environmental pH during Trichothecium roseum (Pers.:Fr.) Link Inoculation of Apple Fruits on the Host Differential Reactive Oxygen Species Metabolism." Antioxidants 10, no. 5: 692.
Pink rot caused by Trichothecium roseum is an important postharvest disease of muskmelon, and caused very serious postharvest losses. In this study, sodium nitroprusside (SNP), an exogenous nitric oxide donor, was applied four times during fruit development to muskmelon. The effect of SNP sprays on pink rot in harvested fruit and the influence on the mechanism of antioxidant enzymes and the phenylpropanoid pathway were investigated. The results showed that SNP sprays significantly reduced decay incidence and lesion area of muskmelon inoculated with T. roseum. SNP sprays induced the production of H2O2 in fruit at harvest, maintained cell membrane integrity, and increased the activity of superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase during storage. In addition, SNP sprays also increased the activity of phenylalanine ammonia‐lyase, cinnamate‐4‐hydroxylase, and 4‐coumaroyl‐CoA ligase, and promoted the accumulation of total phenolic, flavonoid, and lignin content in harvested muskmelon at harvest and during storage. These results suggesting a new possible way of prevention of postharvest pathogens. Practical applications Muskmelon is an important cash crop in the world, due to its unique appearance and intrinsic quality. However, postharvest losses of the muskmelon fruit are serious. Pink rot caused by Trichothecium roseum is one of the main reasons for the postharvest losses of harvested muskmelon. SNP preharvest sprays effectively induced the resistance and reduced decay incidence and lesion area of muskmelon inoculated with T. roseum. Compared with the postharvest treatment, which involves complete exposure, preharvest sprays not only affects the fruit, but also other portions of the plants, which could indirectly affect fruit quality. In addition, the preharvest treatment operation is simple and convenient, and the control effect is better than the postharvest treatment. Therefore, this work may provide a new approach for the control of postharvest diseases of muskmelon.
Bin Wang; Xingfen He; Yang Bi; Hong Jiang; Yi Wang; Xiaoyuan Zheng; Dov Prusky. Preharvest sprays with sodium nitroprusside induce resistance in harvested muskmelon against the pink rot disease. Journal of Food Processing and Preservation 2021, 45, e15339 .
AMA StyleBin Wang, Xingfen He, Yang Bi, Hong Jiang, Yi Wang, Xiaoyuan Zheng, Dov Prusky. Preharvest sprays with sodium nitroprusside induce resistance in harvested muskmelon against the pink rot disease. Journal of Food Processing and Preservation. 2021; 45 (4):e15339.
Chicago/Turabian StyleBin Wang; Xingfen He; Yang Bi; Hong Jiang; Yi Wang; Xiaoyuan Zheng; Dov Prusky. 2021. "Preharvest sprays with sodium nitroprusside induce resistance in harvested muskmelon against the pink rot disease." Journal of Food Processing and Preservation 45, no. 4: e15339.
A novel colorimetric aptasensor based on unmodified gold nanoparticle (AuNPs) and single-strand DNA (ssDNA) aptamer was developed for the rapid and sensitive detection of T-2 toxin. In the absence of T-2, the AuNPs were wrapped by the aptamer to avoid the salt-induced aggregation and the solution remains red. In the presence of T-2, the aptamer was bound with T-2 and released from the surface of AuNPs, resulting in the aggregation of AuNPs under proper salt solution and the color change from red to purple-blue. The aptasensor exhibited a high sensitivity and selectivity for the detection of T-2. The range of linearity and detection limit were 0.1 ng/mL-5000 ng/mL (0.21435 nM-10717.5 nM) and 57.8 pg/mL (0.124 nM), respectively. The aptasensor developed here was applicable to assay T-2 in wheat and corn samples. These results implied that the colorimetric aptasensor was potentially useful in food detection.
Wenwei Zhang; Yanling Wang; Mina Nan; Yongcai Li; Jianmin Yun; Yi Wang; Yang Bi. Novel colorimetric aptasensor based on unmodified gold nanoparticle and ssDNA for rapid and sensitive detection of T-2 toxin. Food Chemistry 2021, 348, 129128 .
AMA StyleWenwei Zhang, Yanling Wang, Mina Nan, Yongcai Li, Jianmin Yun, Yi Wang, Yang Bi. Novel colorimetric aptasensor based on unmodified gold nanoparticle and ssDNA for rapid and sensitive detection of T-2 toxin. Food Chemistry. 2021; 348 ():129128.
Chicago/Turabian StyleWenwei Zhang; Yanling Wang; Mina Nan; Yongcai Li; Jianmin Yun; Yi Wang; Yang Bi. 2021. "Novel colorimetric aptasensor based on unmodified gold nanoparticle and ssDNA for rapid and sensitive detection of T-2 toxin." Food Chemistry 348, no. : 129128.
Postharvest diseases are the primary reason causing postharvest loss of fruits and vegetables. Although fungicides show an effective way to control postharvest diseases, the use of fungicides is gradually being restricted due to safety, environmental pollution, and resistance development in the pathogen. Induced resistance is a new strategy to control postharvest diseases by eliciting immune activity in fruits and vegetables with exogenous physical, chemical, and biological elicitors. After being stimulated by elicitors, fruits and vegetables respond immediately against pathogens. This process is actually a continuous signal transduction, including the generation, transduction, and interaction of signal molecules. Each step of response can lead to corresponding physiological functions, and ultimately induce disease resistance by upregulating the expression of disease resistance genes and activating a variety of metabolic pathways. Signal molecules not only mediate defense response alone, but also interact with other signal transduction pathways to regulate the disease resistance response. Among various signal molecules, the second messenger (reactive oxygen species, nitric oxide, calcium ions) and plant hormones (salicylic acid, jasmonic acid, ethylene, and abscisic acid) play an important role in induced resistance. This article summarizes and reviews the research progress of induced resistance in recent years, and expounds the role of the above-mentioned signal molecules in induced resistance of harvested fruits and vegetables, and prospects for future research.
Bin Wang; Yang Bi. The role of signal production and transduction in induced resistance of harvested fruits and vegetables. Food Quality and Safety 2021, 5, 1 .
AMA StyleBin Wang, Yang Bi. The role of signal production and transduction in induced resistance of harvested fruits and vegetables. Food Quality and Safety. 2021; 5 ():1.
Chicago/Turabian StyleBin Wang; Yang Bi. 2021. "The role of signal production and transduction in induced resistance of harvested fruits and vegetables." Food Quality and Safety 5, no. : 1.
Wound healing is an inherent property of harvested potato tubers. However natural healing process usually needs 2-4 weeks, which increased water loss and pathogen invasion. Therefore, it is necessary to develop a technology to accelerate wound healing processing. Stroby is a biogenic fungicide with induced resistance and it has not been reported whether it can improve the wound ability of potato tubers. Potato plants (cv. Longshu 7) were repeatedly sprayed with 0.4 g L-1 (w/v) Stroby during tuber development, and the effect of preharvest stroby spraying treatment on wound healing in harvested potato tubers was evaluated in this study. The results showed that Stroby sprays reduced weight loss and disease index of harvested tubers inoculated with Fusarium sulphureum. Stroby-treated potato tubers also showed accelerated accumulation and increased thickness of the suberin polyphenolic, suberin polyaliphatic and lignin at wound sites of tubers. As the major substrates of suberin synthesis, cinnamic, caffeic, ferulic and p-coumaric acids were accelerated, and the content of total phenolics, flavonoids and lignin were increased along with increased activity of phenylalanine ammonia-lyase (PLA) at wound sites of harvested tubers. The results suggest that preharvest multiple sprays with Stroby on potato plants could accelerate wound healing of harvested tubers via activated phenylpropanoid pathway.
Xiangzhen Ge; Yan Zhu; Zhicheng Li; Yang Bi; Jing Yang; Junlian Zhang; Dov Prusky. Preharvest multiple fungicide stroby sprays promote wound healing of harvested potato tubers by activating phenylpropanoid metabolism. Postharvest Biology and Technology 2020, 171, 111328 .
AMA StyleXiangzhen Ge, Yan Zhu, Zhicheng Li, Yang Bi, Jing Yang, Junlian Zhang, Dov Prusky. Preharvest multiple fungicide stroby sprays promote wound healing of harvested potato tubers by activating phenylpropanoid metabolism. Postharvest Biology and Technology. 2020; 171 ():111328.
Chicago/Turabian StyleXiangzhen Ge; Yan Zhu; Zhicheng Li; Yang Bi; Jing Yang; Junlian Zhang; Dov Prusky. 2020. "Preharvest multiple fungicide stroby sprays promote wound healing of harvested potato tubers by activating phenylpropanoid metabolism." Postharvest Biology and Technology 171, no. : 111328.
Benzo (1, 2, 3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) is the first synthetic nontoxic chemical elicitor that has induced resistance in fruit and vegetables against postharvest diseases. In this study, we treated artificially wounded muskmelon fruit (Cucumis melo L. cv. Perfect) with 100 mg L-1 BTH. The results indicated that there was a significant accumulation of suberin polyphenolics and lignin at wound sites on the treated fruit as well as a noticeable decrease in weight loss of wounded fruit during healing. The BTH treatment also enhanced the activity of NADPH oxidase and superoxide dismutase, increasing the production of O2•- and H2O2. Moreover, BTH activated the activity of phenylalanine ammonia-lyase, cinnamate-4-hydroxylase, 4-coumarate-CoA ligase, and cinnamyl alcohol dehydrogenase as well as elevating the levels of cinnamic acid, p-coumaric acid, caffeic acid, ferulic acid, sinapic acid, coniferyl alcohol, sinapyl alcohol, and lignin at the wound sites on the treated fruit. When we treated the BTH-treated muskmelons with 50 μmol L-1 diphenylene iodonium (DPI), the treatment partially inhibited the accumulation of polyphenolic substances and lignin at wound sites on the treated fruit, weakened the activity of NADPH oxidase and superoxide dismutase, and reduced the production of O2.- and H2O2. Furthermore, BTH combined with DPI treatment caused lower activity of key enzymes and lower level of metabolites in phenylpropanoid pathway compared with BTH treatment. Considering DPI as a reactive oxygen species inhibitor, we suggest that reactive oxygen species could be a second messenger for activating phenylpropanoid metabolism, as a consequence for promoting polyphenolic substances and lignin accumulation and reducing weight loss in the BTH-treated muskmelons during healing.
Yi Wang; Qian Yang; Hong Jiang; Bin Wang; Yang Bi; Yongcai Li; Dov Prusky. Reactive oxygen species-mediated the accumulation of suberin polyphenolics and lignin at wound sites on muskmelons elicited by benzo (1, 2, 3)-thiadiazole-7-carbothioic acid S-methyl ester. Postharvest Biology and Technology 2020, 170, 111325 .
AMA StyleYi Wang, Qian Yang, Hong Jiang, Bin Wang, Yang Bi, Yongcai Li, Dov Prusky. Reactive oxygen species-mediated the accumulation of suberin polyphenolics and lignin at wound sites on muskmelons elicited by benzo (1, 2, 3)-thiadiazole-7-carbothioic acid S-methyl ester. Postharvest Biology and Technology. 2020; 170 ():111325.
Chicago/Turabian StyleYi Wang; Qian Yang; Hong Jiang; Bin Wang; Yang Bi; Yongcai Li; Dov Prusky. 2020. "Reactive oxygen species-mediated the accumulation of suberin polyphenolics and lignin at wound sites on muskmelons elicited by benzo (1, 2, 3)-thiadiazole-7-carbothioic acid S-methyl ester." Postharvest Biology and Technology 170, no. : 111325.
Amino and thiolated aptamers are the main aptamers used to construct label-free electrochemical impedimetric aptasensors. In this study, the modification performance and electrochemical properties of amino aptamers and thiolated aptamers were studied in the construction of label-free impedimetric sensors. The results showed that the initial modification density of amino aptamers was higher than that of thiol aptamers. Aptamers can recognize and bind OTA to generate electrical signals. The higher the density of aptamer modification was, the better the electric signals were. If only considering the initial modification density, amino aptamers were more suitable for the preparation of aptasensors than thiolated aptamers. However, the modification density of the amino aptamer decreased with the prolonged immersion time in 1 mM HCl solution, which suggests that the stability of this sensor was poor. However, the thiolated aptamer maintained relatively constant density and could be reused. Thus, the thiolated aptasensor had a wide range and good reproducibility and stability for the determination of ochratoxin A (OTA). In addition, this study proved that gold nanoparticles play an important role in signal amplification by increasing the effective gold surface to fix more aptamers in the process of sensor preparation.
Mi-Na Nan; Yang Bi; Hua-Li Xue; Hai-Tao Long; Su-Lin Xue; Lu-Mei Pu; Dov Prusky. Modification performance and electrochemical characteristics of different groups of modified aptamers applied for label-free electrochemical impedimetric sensors. Food Chemistry 2020, 337, 127761 .
AMA StyleMi-Na Nan, Yang Bi, Hua-Li Xue, Hai-Tao Long, Su-Lin Xue, Lu-Mei Pu, Dov Prusky. Modification performance and electrochemical characteristics of different groups of modified aptamers applied for label-free electrochemical impedimetric sensors. Food Chemistry. 2020; 337 ():127761.
Chicago/Turabian StyleMi-Na Nan; Yang Bi; Hua-Li Xue; Hai-Tao Long; Su-Lin Xue; Lu-Mei Pu; Dov Prusky. 2020. "Modification performance and electrochemical characteristics of different groups of modified aptamers applied for label-free electrochemical impedimetric sensors." Food Chemistry 337, no. : 127761.
This study examines the effects of hot water dipping on wound healing of harvested potato tubers and to explore partial mechanisms. The potato ‘cv. Longshu No. 7′ was used as materials, which were artificially wounded and then dipped into 45 °C hot water for 10 min. The wounded tubers were stored at ambient temperature (20−25 °C, RH 70–80%) in dark for wound healing. The weight loss of wounded tubers and the disease index of wounded tubers inoculated with Fusarium sulphureum were determined. The accumulation of suberin poly phenolic, suberin poly aliphatic and lignin at wound sites were observed. The activity of phenylalanine ammonia lyase, peroxidase activity, the content of H2O2, total phenols, flavonoids and lignin were also measured. We found that hot water dipping effectively reduced the weight loss and the disease index of wounded potato tubers. The weight loss of treated tubers was 45.1% lower than the control on the 14 d of healing, and the disease index of treated tubers was 18.2% lower than the control on the 21 d of healing. Hot water dipping significantly stimulated the accumulation of suberin poly phenolic, suberin poly aliphatic and lignin at wound sites. The thickness of suberin poly phenolic, suberin poly aliphatic and lignin cell layers of treated tubers were 28.9%, 23.2% and 18.9% higher than the control on the 21 d of healing, respectively. Moreover, hot water dipping significantly enhanced the activity of phenylalanine ammonia lyase and peroxidase. Meanwhile, the H2O2 content, total phenols, flavonoids and lignin were also significantly promoted at wound sites by hot water dipping treatment during healing. These results suggested that hot water dipping promotes wound healing of potato tubers by activating phenylpropanoid metabolism, increasing H2O2 content and peroxidase activity, promoting the suberin and lignin accumulation at wound sites and decreasing the weight loss and the disease index of tubers during healing.
Ruirui Yang; Ye Han; Zhanhong Han; Sabina Ackah; Zhicheng Li; Yang Bi; Qian Yang; Dov Prusky. Hot water dipping stimulated wound healing of potato tubers. Postharvest Biology and Technology 2020, 167, 111245 .
AMA StyleRuirui Yang, Ye Han, Zhanhong Han, Sabina Ackah, Zhicheng Li, Yang Bi, Qian Yang, Dov Prusky. Hot water dipping stimulated wound healing of potato tubers. Postharvest Biology and Technology. 2020; 167 ():111245.
Chicago/Turabian StyleRuirui Yang; Ye Han; Zhanhong Han; Sabina Ackah; Zhicheng Li; Yang Bi; Qian Yang; Dov Prusky. 2020. "Hot water dipping stimulated wound healing of potato tubers." Postharvest Biology and Technology 167, no. : 111245.
Pichia guilliermondii is a yeast widely found on soil, leaves and fruit surfaces and is known to have a good biocontrol effect on a variety of postharvest diseases of fruits and vegetables. In this study, artificially wounded apple fruits (cv. Fuji) were treated with P. guilliermondii. It was found that P. guilliermondii growth increased rapidly at wound sites of fruit in the early stage of healing and later decreased rapidly. The yeast significantly reduced weight loss of wounded fruit and the disease index of inoculated fruit with Penicillium expansum. On the 5 d of healing, the weight loss and disease index of treated fruits were 25 % and 44 % lower than the control, respectively. P. guilliermondii also promoted the accumulation of O2·− and H2O2, increased the activity of superoxide dismutase, catalase, peroxidase and polyphenol oxidase in fruit wounds. Furthermore, P. guilliermondii activated phenylalnine ammonialyase activity and increased the content of total phenols, flavonoids and lignin in the fruit wounds. It is suggested that postharvest P. guilliermondii treatment could promote the wound-healing of apple fruit by activating the reactive oxygen species and phenylpropane metabolism in the fruit wound.
Xuemei Zhang; Yuanyuan Zong; Zhicheng Li; Ruirui Yang; Zihe Li; Yang Bi; Dov Prusky. Postharvest Pichia guilliermondii treatment promotes wound healing of apple fruits. Postharvest Biology and Technology 2020, 167, 111228 .
AMA StyleXuemei Zhang, Yuanyuan Zong, Zhicheng Li, Ruirui Yang, Zihe Li, Yang Bi, Dov Prusky. Postharvest Pichia guilliermondii treatment promotes wound healing of apple fruits. Postharvest Biology and Technology. 2020; 167 ():111228.
Chicago/Turabian StyleXuemei Zhang; Yuanyuan Zong; Zhicheng Li; Ruirui Yang; Zihe Li; Yang Bi; Dov Prusky. 2020. "Postharvest Pichia guilliermondii treatment promotes wound healing of apple fruits." Postharvest Biology and Technology 167, no. : 111228.
Wound healing ability of potato tubers depends on cultivar. However, there are few researches on the wound healing dynamics of different potato cultivars and reports at biochemical and cellular level. In this study, the healing ability among four major potato cultivars, ‘Atlantic’, ‘Shepody’, ‘Desiree’ and ‘Favorite’ is compared after wounding the tubers artificially. The results indicated that during healing the wounded tubers ‘Atlantic’ had the lowest weight loss and disease index, compared with ‘Shepody’, ‘Desiree’ and ‘Favorita’ which was the highest. ‘Atlantic’ also had the largest accumulation of suberin poly phenolic and lignin at wounded sites of tubers during healing, followed by ‘Shepody’ and ‘Desiree’, and accumulation of ‘Favorita’ was the least. Moreover, ‘Atlantic’ had the highest phenylalanine ammonialyase and peroxidase activities, total phenols, flavonoid and lignin contents, and ABTS+ scavenging ability in the tissue of wound sites during healing, followed by ‘Shepody’, ‘Desiree’ and ‘Favorita’. It is suggested that wound healing ability of potato tubers of different cultivar is strongly affected by the activation of phenylpropanoid metabolism and peroxidase.
Xiaoyuan Zheng; Hong Jiang; Yang Bi; Bin Wang; Tiaolan Wang; Yongcai Li; Di Gong; Yanan Wei; Zhicheng Li; Dov Prusky. Comparison of wound healing abilities of four major cultivars of potato tubers in China. Postharvest Biology and Technology 2020, 164, 111167 .
AMA StyleXiaoyuan Zheng, Hong Jiang, Yang Bi, Bin Wang, Tiaolan Wang, Yongcai Li, Di Gong, Yanan Wei, Zhicheng Li, Dov Prusky. Comparison of wound healing abilities of four major cultivars of potato tubers in China. Postharvest Biology and Technology. 2020; 164 ():111167.
Chicago/Turabian StyleXiaoyuan Zheng; Hong Jiang; Yang Bi; Bin Wang; Tiaolan Wang; Yongcai Li; Di Gong; Yanan Wei; Zhicheng Li; Dov Prusky. 2020. "Comparison of wound healing abilities of four major cultivars of potato tubers in China." Postharvest Biology and Technology 164, no. : 111167.
Black spot caused by Alternaria alternata is one of the important diseases of pear fruit during storage. Isothiocyanates are known as being strong antifungal compounds in vitro against different fungi. The aim of this study was to assess the antifungal effects of the volatile compound 2-phenylethyl isothiocyanate (2-PEITC) against A. alternata in vitro and in pear fruit, and to explore the underlying inhibitory mechanisms. The in vitro results showed that 2-PEITC significantly inhibited spore germination and mycelial growth of A. alternata—the inhibitory effects showed a dose-dependent pattern and the minimum inhibitory concentration (MIC) was 1.22 mM. The development of black spot rot on the pear fruit inoculated with A. alternata was also significantly decreased by 2-PEITC fumigation. At 1.22 mM concentration, the lesion diameter was only 39% of that in the control fruit at 7 days after inoculation. Further results of the leakage of electrolyte, increase of intracellular OD260, and propidium iodide (PI) staining proved that 2-PEITC broke cell membrane permeability of A. alternata. Moreover, 2-PEITC treatment significantly decreased alternariol (AOH), alternariolmonomethyl ether (AME), altenuene (ALT), and tentoxin (TEN) contents of A. alternata. Taken together, these data suggest that the mechanisms underlying the antifungal effect of 2-PEITC against A. alternata might be via reduction in toxin content and breakdown of cell membrane integrity.
Miao Zhang; Yongcai Li; Yang Bi; Tiaolan Wang; Yupeng Dong; Qian Yang; Tingting Zhang. 2-Phenylethyl Isothiocyanate Exerts Antifungal Activity against Alternaria alternata by Affecting Membrane Integrity and Mycotoxin Production. Toxins 2020, 12, 124 .
AMA StyleMiao Zhang, Yongcai Li, Yang Bi, Tiaolan Wang, Yupeng Dong, Qian Yang, Tingting Zhang. 2-Phenylethyl Isothiocyanate Exerts Antifungal Activity against Alternaria alternata by Affecting Membrane Integrity and Mycotoxin Production. Toxins. 2020; 12 (2):124.
Chicago/Turabian StyleMiao Zhang; Yongcai Li; Yang Bi; Tiaolan Wang; Yupeng Dong; Qian Yang; Tingting Zhang. 2020. "2-Phenylethyl Isothiocyanate Exerts Antifungal Activity against Alternaria alternata by Affecting Membrane Integrity and Mycotoxin Production." Toxins 12, no. 2: 124.
T-2 toxin at low concentrations can induce ROS accumulation and modulate host resistance in plants. NOX plays crucial roles in ROS production and is regulated by Ca2+ via direct binding to EF-hand motifs. In this study, the effect of EGTA (Ca2+ chelating agent) on the expression and enzymatic activity of NOX, as well as the activities and corresponding gene expressions involved in ROS metabolism and cell membrane integrity, were investigated in treated slices. Results indicated that EGTA treatment significantly affected gene expression and activity of NOX, and reduced ROS accumulation and cell membrane integrity and the enzymatic activities and gene expression involved in ROS metabolism when exposed to treatment. The addition of exogenous Ca2+ restored the initial relative transcript abundance, ROS accumulation and their activities. Results suggest that Ca2+ affected by EGTA plays a crucial role in NOX activity regulation, ultimately affecting ROS metabolism in slices induced by T-2 toxin.
Zhang Rui; Xue Huali; Si Min; Bi Yang; Nan Mina; Zong Yuanyuan; Long Haitao; Dov Prusky; Cheng Xiaoyan. Mechanism of Ca2+-mediated NOX modulated in ROS metabolism induced by T-2 toxin in potato tuber. Food Chemistry 2020, 317, 126416 .
AMA StyleZhang Rui, Xue Huali, Si Min, Bi Yang, Nan Mina, Zong Yuanyuan, Long Haitao, Dov Prusky, Cheng Xiaoyan. Mechanism of Ca2+-mediated NOX modulated in ROS metabolism induced by T-2 toxin in potato tuber. Food Chemistry. 2020; 317 ():126416.
Chicago/Turabian StyleZhang Rui; Xue Huali; Si Min; Bi Yang; Nan Mina; Zong Yuanyuan; Long Haitao; Dov Prusky; Cheng Xiaoyan. 2020. "Mechanism of Ca2+-mediated NOX modulated in ROS metabolism induced by T-2 toxin in potato tuber." Food Chemistry 317, no. : 126416.
Trichothecium roseum is a harmful postharvest fungus causing serious damage, together with the secretion of insidious mycotoxins, on apples, melons, and other important fruits. Cuminal, a predominant component of Cuminum cyminum essential oil has proven to successfully inhibit the growth of T. roseum in vitro and in vivo. Electron microscopic observations revealed cuminal exposure impaired the fungal morphology and ultrastructure, particularly the plasmalemma. Transcriptome and proteome analysis was used to investigate the responses of T. roseum to exposure of cuminal. In total, 2825 differentially expressed transcripts (1516 up and 1309 down) and 225 differentially expressed proteins (90 up and 135 down) were determined. Overall, notable parts of these differentially expressed genes functionally belong to subcellular localities of the membrane system and cytosol, along with ribosomes, mitochondria and peroxisomes. According to the localization analysis and the biological annotation of these genes, carbohydrate and lipids metabolism, redox homeostasis, and asexual reproduction were among the most enriched gene ontology (GO) terms. Biological pathway enrichment analysis showed that lipids and amino acid degradation, ATP-binding cassette transporters, membrane reconstitution, mRNA surveillance pathway and peroxisome were elevated, whereas secondary metabolite biosynthesis, cell cycle, and glycolysis/gluconeogenesis were down regulated. Further integrated omics analysis showed that cuminal exposure first impaired the polarity of the cytoplasmic membrane and then triggered the reconstitution and dysfunction of fungal plasmalemma, resulting in handicapped nutrient procurement of the cells. Consequently, fungal cells showed starvation stress with limited carbohydrate metabolism, resulting a metabolic shift to catabolism of the cell’s own components in response to the stress. Additionally, these predicaments brought about oxidative stress, which, in collaboration with the starvation, damaged certain critical organelles such as mitochondria. Such degeneration, accompanied by energy deficiency, suppressed the biosynthesis of essential proteins and inhibited fungal growth.
Zhong Zhang; Wenting Zhang; Yang Bi; Ye Han; Yuanyuan Zong; Dov Prusky. Cuminal Inhibits Trichothecium roseum Growth by Triggering Cell Starvation: Transcriptome and Proteome Analysis. Microorganisms 2020, 8, 256 .
AMA StyleZhong Zhang, Wenting Zhang, Yang Bi, Ye Han, Yuanyuan Zong, Dov Prusky. Cuminal Inhibits Trichothecium roseum Growth by Triggering Cell Starvation: Transcriptome and Proteome Analysis. Microorganisms. 2020; 8 (2):256.
Chicago/Turabian StyleZhong Zhang; Wenting Zhang; Yang Bi; Ye Han; Yuanyuan Zong; Dov Prusky. 2020. "Cuminal Inhibits Trichothecium roseum Growth by Triggering Cell Starvation: Transcriptome and Proteome Analysis." Microorganisms 8, no. 2: 256.