This page has only limited features, please log in for full access.

Dr. Karel Dolezal
Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany, The Czech Academy of Sciences, Šlechtitelů 27, 78371, Olomouc, Czech Republic

Basic Info


Research Keywords & Expertise

0 Chromatography
0 Mass Spectrometry
0 cytokinins
0 Plant hormones
0 Auxins

Fingerprints

cytokinins
Chromatography
Mass Spectrometry
Plant hormones
Auxins

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 29 July 2021 in Catalysts
Reads 0
Downloads 0

1,3-Oxazole and 4,5-dihydro-1,3-oxazole are common structural motifs in naturally occurring peptides. A series of vanadium complexes were synthesized using VCl3(THF)3 and methyl substituted (4,5-dihydro-1,3-oxazol-2-yl)-1,3-oxazoles as ligands and analyzed using NMR and MS methods. The complexes were found to be active catalysts both in ethylene polymerization and ethylene-norbornene copolymerization. The position of methyl substituent in the ligand has considerable impact on the performance of (co)polymerization reaction, as well as on the microstructure, and thus physical properties of the obtained copolymers.

ACS Style

Wioletta Ochędzan-Siodłak; Dawid Siodłak; Karolina Banaś; Katarzyna Halikowska; Sławomir Wierzba; Karel Doležal. Naturally Occurring Oxazole Structural Units as Ligands of Vanadium Catalysts for Ethylene-Norbornene (Co)polymerization. Catalysts 2021, 11, 923 .

AMA Style

Wioletta Ochędzan-Siodłak, Dawid Siodłak, Karolina Banaś, Katarzyna Halikowska, Sławomir Wierzba, Karel Doležal. Naturally Occurring Oxazole Structural Units as Ligands of Vanadium Catalysts for Ethylene-Norbornene (Co)polymerization. Catalysts. 2021; 11 (8):923.

Chicago/Turabian Style

Wioletta Ochędzan-Siodłak; Dawid Siodłak; Karolina Banaś; Katarzyna Halikowska; Sławomir Wierzba; Karel Doležal. 2021. "Naturally Occurring Oxazole Structural Units as Ligands of Vanadium Catalysts for Ethylene-Norbornene (Co)polymerization." Catalysts 11, no. 8: 923.

Chapter
Published: 03 May 2021 in Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture
Reads 0
Downloads 0

Topolins are a relatively recent discovery, following search for viable alternatives to 6-benzylaminopurine (BAP) which while effective and affordable has important disadvantages for certain crops. This chapter reviews some biochemical and technical aspects of topolin metabolism in relation to in vitro plant micropropagation.

ACS Style

Karel Doležal; Magdalena Bryksová. Topolin Metabolism and Its Implications for In Vitro Plant Micropropagation. Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture 2021, 49 -58.

AMA Style

Karel Doležal, Magdalena Bryksová. Topolin Metabolism and Its Implications for In Vitro Plant Micropropagation. Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture. 2021; ():49-58.

Chicago/Turabian Style

Karel Doležal; Magdalena Bryksová. 2021. "Topolin Metabolism and Its Implications for In Vitro Plant Micropropagation." Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture , no. : 49-58.

Original paper
Published: 09 April 2021 in Plant Growth Regulation
Reads 0
Downloads 0

Somatic embryogenesis in Coffea spp. is important for mass production and genetic engineering. Coffee is an exceptional case, as somatic embryogenesis can be induced by applying a cytokinin such as 2iP as the only plant growth regulator. 1-(2-(2-Hydroxyethyl)phenyl)-3-(3-(trifluoromethoxy)phenyl)urea (3TFM-2HE) and 1-(3-bromo-5-(trifluoromethoxy)phenyl)-3-(2-(2-hydroxyethyl)phenyl)urea (3TFM,5Br-2HE) are two newly designed cytokinin oxidase/dehydrogenase inhibitors (CKX) from the diphenylurea group. We used a Coffea arabica leaf disks bioassay to demonstrate the indirect somatic embryo induction potential of these compounds. The leaf disks were incubated on modified Murashige and Skoog (MS) semi-solid medium in which 3TFM-2HE or 3TFM,5Br-2HE were combined with N6-(2-isopentenyl)adenine (2iP). Although these compounds do not possess intrinsic cytokinin activity, they enhanced the activity of 2iP, resulting in direct somatic embryogenesis after seven weeks. The best results were obtained with 1 µM 3TFM-2HE and 5 or 10 µM 2iP. Maturation of somatic embryos into fully developed plants took place on medium supplemented with 0.5 µM kinetin and the somatic embryos developed true leaves and a root system.

ACS Style

Nino Murvanidze; Jaroslav Nisler; Olivier Leroux; Stefaan P. O. Werbrouck. Cytokinin oxidase/dehydrogenase inhibitors stimulate 2iP to induce direct somatic embryogenesis in Coffea arabica. Plant Growth Regulation 2021, 94, 195 -200.

AMA Style

Nino Murvanidze, Jaroslav Nisler, Olivier Leroux, Stefaan P. O. Werbrouck. Cytokinin oxidase/dehydrogenase inhibitors stimulate 2iP to induce direct somatic embryogenesis in Coffea arabica. Plant Growth Regulation. 2021; 94 (2):195-200.

Chicago/Turabian Style

Nino Murvanidze; Jaroslav Nisler; Olivier Leroux; Stefaan P. O. Werbrouck. 2021. "Cytokinin oxidase/dehydrogenase inhibitors stimulate 2iP to induce direct somatic embryogenesis in Coffea arabica." Plant Growth Regulation 94, no. 2: 195-200.

Paper
Published: 25 February 2021 in The Analyst
Reads 0
Downloads 0

The distribution of target phytohormones is highlighted in wounded Arabidopsis leaves, the differences in their abundance are determined, and the correlations between them are analysed using DESI-MSI analysis.

ACS Style

Chao Zhang; Asta Žukauskaitė; Ivan Petřík; Aleš Pěnčík; Martin Hönig; Jiří Grúz; Jitka Široká; Ondřej Novák; Karel Doležal. In situ characterisation of phytohormones from wounded Arabidopsis leaves using desorption electrospray ionisation mass spectrometry imaging. The Analyst 2021, 146, 2653 -2663.

AMA Style

Chao Zhang, Asta Žukauskaitė, Ivan Petřík, Aleš Pěnčík, Martin Hönig, Jiří Grúz, Jitka Široká, Ondřej Novák, Karel Doležal. In situ characterisation of phytohormones from wounded Arabidopsis leaves using desorption electrospray ionisation mass spectrometry imaging. The Analyst. 2021; 146 (8):2653-2663.

Chicago/Turabian Style

Chao Zhang; Asta Žukauskaitė; Ivan Petřík; Aleš Pěnčík; Martin Hönig; Jiří Grúz; Jitka Široká; Ondřej Novák; Karel Doležal. 2021. "In situ characterisation of phytohormones from wounded Arabidopsis leaves using desorption electrospray ionisation mass spectrometry imaging." The Analyst 146, no. 8: 2653-2663.

Regular article
Published: 12 January 2021 in New Phytologist
Reads 0
Downloads 0

In a world that will rely increasingly on efficient plant growth for sufficient food, it is important to learn about natural mechanisms of phytohormone action. In this work, the introduction of a fluorophore to an auxin molecule represents a sensitive and non‐invasive method to directly visualize auxin localization with high spatiotemporal resolution. The state‐of‐the‐art multidisciplinary approaches of genetic and chemical biology analysis together with live cell imaging, liquid chromatography‐mass spectrometry (LC‐MS) and surface plasmon resonance (SPR) methods were employed for the characterization of auxin‐related biological activity, distribution and stability of the presented compounds in Arabidopsis thaliana. Despite partial metabolization in vivo, these fluorescent auxins display an uneven and dynamic distribution leading to the formation of fluorescence maxima in tissues known to concentrate natural auxin, such as the concave side of the apical hook. Importantly, their distribution is altered in response to different exogenous stimuli in both roots and shoots. Moreover, we characterized the subcellular localization of the fluorescent auxin analogues as being present in the endoplasmic reticulum and endosomes. Our work provides powerful tools to visualize auxin distribution within different plant tissues at cellular or subcellular levels and in response to internal and environmental stimuli during plant development.

ACS Style

Barbora Pařízková; Asta Žukauskaitė; Thomas Vain; Peter Grones; Sara Raggi; Martin F. Kubeš; Martin Kieffer; Siamsa M. Doyle; Miroslav Strnad; Stefan Kepinski; Richard Napier; Karel Doležal; Stéphanie Robert; Ondřej Novák. New fluorescent auxin probes visualise tissue‐specific and subcellular distributions of auxin in Arabidopsis. New Phytologist 2021, 230, 535 -549.

AMA Style

Barbora Pařízková, Asta Žukauskaitė, Thomas Vain, Peter Grones, Sara Raggi, Martin F. Kubeš, Martin Kieffer, Siamsa M. Doyle, Miroslav Strnad, Stefan Kepinski, Richard Napier, Karel Doležal, Stéphanie Robert, Ondřej Novák. New fluorescent auxin probes visualise tissue‐specific and subcellular distributions of auxin in Arabidopsis. New Phytologist. 2021; 230 (2):535-549.

Chicago/Turabian Style

Barbora Pařízková; Asta Žukauskaitė; Thomas Vain; Peter Grones; Sara Raggi; Martin F. Kubeš; Martin Kieffer; Siamsa M. Doyle; Miroslav Strnad; Stefan Kepinski; Richard Napier; Karel Doležal; Stéphanie Robert; Ondřej Novák. 2021. "New fluorescent auxin probes visualise tissue‐specific and subcellular distributions of auxin in Arabidopsis." New Phytologist 230, no. 2: 535-549.

Journal article
Published: 30 December 2020 in Agronomy
Reads 0
Downloads 0

Three cytokinin derivatives (CKd) designated as RR-G, RR-O, and RR-V applied by foliar spraying at tillering, and one compound previously described as a cytokinin antagonist (CKa) designated as RR-P applied as a seed coating were tested in winter wheat and spring barley in field trial experiments. The aim of the study was to examine the influence of the compounds that were tested on the number of productive tillers, grain yield, and endogenous CK content. With the exception of the compound RR-V, the measured parameters clearly showed the stimulatory effects of CKd on tillering and grain yield in spring barley and winter wheat. The RR-V showed a stimulatory effect on the number of productive tillers and yield in spring barley, but not in winter wheat. Although in winter wheat CKa stimulated both the number of productive tillers and the grain yield, there was an inhibitory effect in terms of the number of productive tillers observed in spring barley. The results of the endogenous cytokinin analysis suggested, among others, the importance of the role of isopentenyl-adenine types of cytokinins in the tillering of spring barley. In conclusion, the cytokinin derivative compounds with an agonistic or antagonistic role showed strong potential for application in the future development of plant growth regulators.

ACS Style

Radoslav Koprna; Jan F. Humplík; Zdeněk Špíšek; Magdaléna Bryksová; Marek Zatloukal; Václav Mik; Ondřej Novák; Jaroslav Nisler; Karel Doležal. Improvement of Tillering and Grain Yield by Application of Cytokinin Derivatives in Wheat and Barley. Agronomy 2020, 11, 67 .

AMA Style

Radoslav Koprna, Jan F. Humplík, Zdeněk Špíšek, Magdaléna Bryksová, Marek Zatloukal, Václav Mik, Ondřej Novák, Jaroslav Nisler, Karel Doležal. Improvement of Tillering and Grain Yield by Application of Cytokinin Derivatives in Wheat and Barley. Agronomy. 2020; 11 (1):67.

Chicago/Turabian Style

Radoslav Koprna; Jan F. Humplík; Zdeněk Špíšek; Magdaléna Bryksová; Marek Zatloukal; Václav Mik; Ondřej Novák; Jaroslav Nisler; Karel Doležal. 2020. "Improvement of Tillering and Grain Yield by Application of Cytokinin Derivatives in Wheat and Barley." Agronomy 11, no. 1: 67.

Journal article
Published: 25 December 2020 in Plants
Reads 0
Downloads 0

Modifying the cytokinin content in plants is a means of improving plant productivity. Here, we report the development and biological activity of compound TD-K (1-(furan-2-ylmethyl)-3-(1,2,3-thiadiazol-5-yl)urea)which is related to thidiazuron. TD-K—which exhibited extremely high antisenescence activity in the wheat leaf bioassay—and INCYDE (2-chloro-6-(3-methoxyphenyl)aminopurine)—a plant growth regulator reported to inhibit cytokinin oxidase/dehydrogenase (CKX), an enzyme involved in the degradation of the plant hormone cytokinin—were selected for investigation of their effects on the model plant Rapid Cycling Brassica rapa (RCBr). We monitored the expression of BrCKX and isopentenyl transferase (BrIPT), which codes for the key cytokinin biosynthesis enzyme, in developing leaves following INCYDE and TD-K application. Growth room experiments revealed that INCYDE increased RCBr seed yield per plant, but only when applied multiple times and when grown in 5 mM KNO3. Expression in control leaves showed transient, high levels of expression of BrCKX and BrIPT at true leaf appearance. Following INCYDE application, there was a rapid and strong upregulation of BrCKX3, and a transient downregulation of BrIPT1 and BrIPT3. Interestingly, the upregulation of BrCKX3 persisted in a milder form throughout the course of the experiment (16 days). TD-K also upregulated BrCKX3. However, in contrast to INCYDE, this effect disappeared after two days. These results suggest that both compounds (CKX inhibitor and cytokinin TD-K) influenced cytokinin homeostasis in RCBr leaves, but with different mechanisms.

ACS Style

Matthew J. van van Voorthuizen; Jaroslav Nisler; Jiancheng Song; Lukáš Spíchal; Paula E. Jameson. Targeting Cytokinin Homeostasis in Rapid Cycling Brassica rapa with Plant Growth Regulators INCYDE and TD-K. Plants 2020, 10, 39 .

AMA Style

Matthew J. van van Voorthuizen, Jaroslav Nisler, Jiancheng Song, Lukáš Spíchal, Paula E. Jameson. Targeting Cytokinin Homeostasis in Rapid Cycling Brassica rapa with Plant Growth Regulators INCYDE and TD-K. Plants. 2020; 10 (1):39.

Chicago/Turabian Style

Matthew J. van van Voorthuizen; Jaroslav Nisler; Jiancheng Song; Lukáš Spíchal; Paula E. Jameson. 2020. "Targeting Cytokinin Homeostasis in Rapid Cycling Brassica rapa with Plant Growth Regulators INCYDE and TD-K." Plants 10, no. 1: 39.

Journal article
Published: 26 November 2020 in Plants
Reads 0
Downloads 0

In Africa and Asia, members of the genus Brachystelma are well-known for their diverse uses, especially their medicinal and nutritional values. However, the use of many Brachystelma species as a valuable resource is generally accompanied by the concern of over-exploitation attributed to their slow growth and general small size. The aim of the current study was to establish efficient micropropagation protocols for three Brachystelma species, namely Brachystelma ngomense (endangered), Brachystelma pulchellum (vulnerable) and Brachystelma pygmaeum (least concern), as a means of ensuring their conservation and survival. This was achieved using nodal segments (~10 mm in length) as the source of explants in the presence of different concentrations of three cytokinins (CK) namely N6-benzyladenine (BA), isopentenyladenine (iP) and meta-topolin riboside (mTR), over a period of 6 weeks. The highest (25 µM) concentration of cytokinin treatments typically resulted in significantly higher shoot proliferation. However, each species differed in its response to specific CK: the optimal concentrations were 25 µM mTR, 25 µM iP and 25 µM BA for Brachystelma ngomense, Brachystelma pulchellum and Brachystelma pygmaeum, respectively. During the in vitro propagation, both Brachystelma ngomense and Brachystelma pygmaeum rooted poorly while regenerated Brachystelma pulchellum generally lacked roots regardless of the CK treatments. Following pulsing (dipping) treatment of in vitro-regenerated shoots with indole-3-butyric acid (IBA), acclimatization of all three Brachystelma species remained extremely limited due to poor rooting ex vitro. To the best of our knowledge, the current protocols provide the first successful report for these Brachystelma species. However, further research remains essential to enhance the efficiency of the devised protocol.

ACS Style

Nqobile P. Hlophe; Adeyemi O. Aremu; Karel Doležal; Johannes Van Staden; Jeffrey F. Finnie. Cytokinin-Facilitated Plant Regeneration of Three Brachystelma Species with Different Conservation Status. Plants 2020, 9, 1657 .

AMA Style

Nqobile P. Hlophe, Adeyemi O. Aremu, Karel Doležal, Johannes Van Staden, Jeffrey F. Finnie. Cytokinin-Facilitated Plant Regeneration of Three Brachystelma Species with Different Conservation Status. Plants. 2020; 9 (12):1657.

Chicago/Turabian Style

Nqobile P. Hlophe; Adeyemi O. Aremu; Karel Doležal; Johannes Van Staden; Jeffrey F. Finnie. 2020. "Cytokinin-Facilitated Plant Regeneration of Three Brachystelma Species with Different Conservation Status." Plants 9, no. 12: 1657.

Journal article
Published: 30 October 2020 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Leaf senescence, accompanied by chlorophyll breakdown, chloroplast degradation and inhibition of photosynthesis, can be suppressed by an exogenous application of cytokinins. Two aromatic cytokinin arabinosides (6-benzylamino-9-β-d-arabinofuranosylpurines; BAPAs), 3-hydroxy- (3OHBAPA) and 3-methoxy- (3MeOBAPA) derivatives, have recently been found to possess high anti-senescence activity. Interestingly, their effect on the maintenance of chlorophyll content and maximal quantum yield of photosystem II (PSII) in detached dark-adapted leaves differed quantitatively in wheat (Triticum aestivum L. cv. Aranka) and Arabidopsis (Arabidopsisthaliana L. (Col-0)). In this work, we have found that the anti-senescence effects of 3OHBAPA and 3MeOBAPA in wheat and Arabidopsis also differ in other parameters, including the maintenance of carotenoid content and chloroplasts, rate of reduction of primary electron acceptor of PSII (QA) as well as electron transport behind QA, and partitioning of absorbed light energy in light-adapted leaves. In wheat, 3OHBAPA had a higher protective effect than 3MeOBAPA, whereas in Arabidopsis, 3MeOBAPA was the more efficient derivative. We have found that the different anti-senescent activity of 3OHBAPA and 3MeOBAPA was coupled to different ethylene production in the treated leaves: the lower the ethylene production, the higher the anti-senescence activity. 3OHBAPA and 3MeOBAPA also efficiently protected the senescing leaves of wheat and Arabidopsis against oxidative damage induced by both H2O2 and high-light treatment, which could also be connected with the low level of ethylene production.

ACS Style

Zuzana Kučerová; Marek Rác; Jaromír Mikulík; Ondřej Plíhal; Pavel Pospíšil; Magdaléna Bryksová; Michaela Sedlářová; Karel Doležal; Martina Špundová. The Anti-Senescence Activity of Cytokinin Arabinosides in Wheat and Arabidopsis Is Negatively Correlated with Ethylene Production. International Journal of Molecular Sciences 2020, 21, 8109 .

AMA Style

Zuzana Kučerová, Marek Rác, Jaromír Mikulík, Ondřej Plíhal, Pavel Pospíšil, Magdaléna Bryksová, Michaela Sedlářová, Karel Doležal, Martina Špundová. The Anti-Senescence Activity of Cytokinin Arabinosides in Wheat and Arabidopsis Is Negatively Correlated with Ethylene Production. International Journal of Molecular Sciences. 2020; 21 (21):8109.

Chicago/Turabian Style

Zuzana Kučerová; Marek Rác; Jaromír Mikulík; Ondřej Plíhal; Pavel Pospíšil; Magdaléna Bryksová; Michaela Sedlářová; Karel Doležal; Martina Špundová. 2020. "The Anti-Senescence Activity of Cytokinin Arabinosides in Wheat and Arabidopsis Is Negatively Correlated with Ethylene Production." International Journal of Molecular Sciences 21, no. 21: 8109.

Journal article
Published: 18 September 2020 in Journal of Experimental Botany
Reads 0
Downloads 0

Increasing crop productivity is our major challenge if we are to meet global needs for food, fodder and fuel. Controlling the content of the plant hormone cytokinin is a method of improving plant productivity. Cytokinin oxidase/dehydrogenase (CKO/CKX) is a major target in this regard because it degrades cytokinins. Here, we describe the synthesis and biological activities of new CKX inhibitors derived mainly from diphenylurea. They were tested on four CKX isoforms from maize and Arabidopsis, where the best compounds showed IC50 values in the 10–8 M concentration range. The binding mode of the most efficient inhibitors was characterized from high-resolution crystal complexed structures. Although these compounds do not possess intrinsic cytokinin activity, we have demonstrated their tremendous potential for use in the plant tissue culture industry as well as in agriculture. We have identified a key substance, compound 19, which not only increases stress resistance and seed yield in Arabidopsis, but also improves the yield of wheat, barley and rapeseed grains under field conditions. Our findings reveal that modulation of cytokinin levels via CKX inhibition can positively affect plant growth, development and yield, and prove that CKX inhibitors can be an attractive target in plant biotechnology and agriculture.

ACS Style

Jaroslav Nisler; David Kopečný; Zuzana Pěkná; Radka Končitíková; Radoslav Koprna; Nino Murvanidze; Stefaan P O Werbrouck; Libor Havlíček; Nuria De Diego; Martina Kopečná; Zdeněk Wimmer; Pierre Briozzo; Solange Moréra; David Zalabák; Lukáš Spíchal; Miroslav Strnad. Diphenylurea-derived cytokinin oxidase/dehydrogenase inhibitors for biotechnology and agriculture. Journal of Experimental Botany 2020, 72, 355 -370.

AMA Style

Jaroslav Nisler, David Kopečný, Zuzana Pěkná, Radka Končitíková, Radoslav Koprna, Nino Murvanidze, Stefaan P O Werbrouck, Libor Havlíček, Nuria De Diego, Martina Kopečná, Zdeněk Wimmer, Pierre Briozzo, Solange Moréra, David Zalabák, Lukáš Spíchal, Miroslav Strnad. Diphenylurea-derived cytokinin oxidase/dehydrogenase inhibitors for biotechnology and agriculture. Journal of Experimental Botany. 2020; 72 (2):355-370.

Chicago/Turabian Style

Jaroslav Nisler; David Kopečný; Zuzana Pěkná; Radka Končitíková; Radoslav Koprna; Nino Murvanidze; Stefaan P O Werbrouck; Libor Havlíček; Nuria De Diego; Martina Kopečná; Zdeněk Wimmer; Pierre Briozzo; Solange Moréra; David Zalabák; Lukáš Spíchal; Miroslav Strnad. 2020. "Diphenylurea-derived cytokinin oxidase/dehydrogenase inhibitors for biotechnology and agriculture." Journal of Experimental Botany 72, no. 2: 355-370.

Journal article
Published: 27 August 2020 in Nature Communications
Reads 0
Downloads 0

Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses.

ACS Style

Ioanna Antoniadi; Ondřej Novák; Zuzana Gelová; Alexander Johnson; Ondřej Plíhal; Radim Simerský; Václav Mik; Thomas Vain; Eduardo Mateo-Bonmatí; Michal Karady; Markéta Pernisová; Lenka Plačková; Korawit Opassathian; Jan Hejátko; Stéphanie Robert; Jiří Friml; Karel Doležal; Karin Ljung; Colin Turnbull. Cell-surface receptors enable perception of extracellular cytokinins. Nature Communications 2020, 11, 1 -10.

AMA Style

Ioanna Antoniadi, Ondřej Novák, Zuzana Gelová, Alexander Johnson, Ondřej Plíhal, Radim Simerský, Václav Mik, Thomas Vain, Eduardo Mateo-Bonmatí, Michal Karady, Markéta Pernisová, Lenka Plačková, Korawit Opassathian, Jan Hejátko, Stéphanie Robert, Jiří Friml, Karel Doležal, Karin Ljung, Colin Turnbull. Cell-surface receptors enable perception of extracellular cytokinins. Nature Communications. 2020; 11 (1):1-10.

Chicago/Turabian Style

Ioanna Antoniadi; Ondřej Novák; Zuzana Gelová; Alexander Johnson; Ondřej Plíhal; Radim Simerský; Václav Mik; Thomas Vain; Eduardo Mateo-Bonmatí; Michal Karady; Markéta Pernisová; Lenka Plačková; Korawit Opassathian; Jan Hejátko; Stéphanie Robert; Jiří Friml; Karel Doležal; Karin Ljung; Colin Turnbull. 2020. "Cell-surface receptors enable perception of extracellular cytokinins." Nature Communications 11, no. 1: 1-10.

Journal article
Published: 27 August 2020 in Nature Communications
Reads 0
Downloads 0

Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via a two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localization of the receptors proposed the endoplasmic reticulum (ER) membrane as a principal cytokinin perception site, while study of cytokinin transport pointed to the plasma membrane (PM)-mediated cytokinin signalling. Here, by detailed monitoring of subcellular localizations of the fluorescently labelled natural cytokinin probe and the receptor ARABIDOPSIS HISTIDINE KINASE 4 (CRE1/AHK4) fused to GFP reporter, we show that pools of the ER-located cytokinin receptors can enter the secretory pathway and reach the PM in cells of the root apical meristem, and the cell plate of dividing meristematic cells. Brefeldin A (BFA) experiments revealed vesicular recycling of the receptor and its accumulation in BFA compartments. We provide a revised view on cytokinin signalling and the possibility of multiple sites of perception at PM and ER.

ACS Style

Karolina Kubiasová; Juan Carlos Montesinos; Olga Šamajová; Jaroslav Nisler; Václav Mik; Hana Semerádová; Lucie Plíhalová; Ondřej Novák; Peter Marhavý; Nicola Cavallari; David Zalabák; Karel Berka; Karel Doležal; Petr Galuszka; Jozef Šamaj; Miroslav Strnad; Eva Benková; Ondřej Plíhal; Lukáš Spíchal. Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. Nature Communications 2020, 11, 1 -11.

AMA Style

Karolina Kubiasová, Juan Carlos Montesinos, Olga Šamajová, Jaroslav Nisler, Václav Mik, Hana Semerádová, Lucie Plíhalová, Ondřej Novák, Peter Marhavý, Nicola Cavallari, David Zalabák, Karel Berka, Karel Doležal, Petr Galuszka, Jozef Šamaj, Miroslav Strnad, Eva Benková, Ondřej Plíhal, Lukáš Spíchal. Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum. Nature Communications. 2020; 11 (1):1-11.

Chicago/Turabian Style

Karolina Kubiasová; Juan Carlos Montesinos; Olga Šamajová; Jaroslav Nisler; Václav Mik; Hana Semerádová; Lucie Plíhalová; Ondřej Novák; Peter Marhavý; Nicola Cavallari; David Zalabák; Karel Berka; Karel Doležal; Petr Galuszka; Jozef Šamaj; Miroslav Strnad; Eva Benková; Ondřej Plíhal; Lukáš Spíchal. 2020. "Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum." Nature Communications 11, no. 1: 1-11.

Review
Published: 22 August 2020 in Biomolecules
Reads 0
Downloads 0

Cytokinins (CKs) are a chemically diverse class of plant growth regulators, exhibiting wide-ranging actions on plant growth and development, hence their exploitation in agriculture for crop improvement and management. Their coordinated regulatory effects and cross-talk interactions with other phytohormones and signaling networks are highly sophisticated, eliciting and controlling varied biological processes at the cellular to organismal levels. In this review, we briefly introduce the mode of action and general molecular biological effects of naturally occurring CKs before highlighting the great variability in the response of fruit crops to CK-based innovations. We present a comprehensive compilation of research linked to the application of CKs in non-model crop species in different phases of fruit production and management. By doing so, it is clear that the effects of CKs on fruit set, development, maturation, and ripening are not necessarily generic, even for cultivars within the same species, illustrating the magnitude of yet unknown intricate biochemical and genetic mechanisms regulating these processes in different fruit crops. Current approaches using genomic-to-metabolomic analysis are providing new insights into the in planta mechanisms of CKs, pinpointing the underlying CK-derived actions that may serve as potential targets for improving crop-specific traits and the development of new solutions for the preharvest and postharvest management of fruit crops. Where information is available, CK molecular biology is discussed in the context of its present and future implications in the applications of CKs to fruits of horticultural significance.

ACS Style

Adeyemi O. Aremu; Olaniyi A. Fawole; Nokwanda P. Makunga; Nqobile A. Masondo; Mack Moyo; Nana M. D. Buthelezi; Stephen O. Amoo; Lukáš Spíchal; Karel Doležal. Applications of Cytokinins in Horticultural Fruit Crops: Trends and Future Prospects. Biomolecules 2020, 10, 1222 .

AMA Style

Adeyemi O. Aremu, Olaniyi A. Fawole, Nokwanda P. Makunga, Nqobile A. Masondo, Mack Moyo, Nana M. D. Buthelezi, Stephen O. Amoo, Lukáš Spíchal, Karel Doležal. Applications of Cytokinins in Horticultural Fruit Crops: Trends and Future Prospects. Biomolecules. 2020; 10 (9):1222.

Chicago/Turabian Style

Adeyemi O. Aremu; Olaniyi A. Fawole; Nokwanda P. Makunga; Nqobile A. Masondo; Mack Moyo; Nana M. D. Buthelezi; Stephen O. Amoo; Lukáš Spíchal; Karel Doležal. 2020. "Applications of Cytokinins in Horticultural Fruit Crops: Trends and Future Prospects." Biomolecules 10, no. 9: 1222.

Review
Published: 29 May 2020 in Biomolecules
Reads 0
Downloads 0

Cytokinins and their sugar or non-sugar conjugates are very active growth-promoting factors in plants, although they occur at very low concentrations. These compounds have been identified in numerous plant species. This review predominantly focuses on 9-substituted adenine-based cytokinin conjugates, both artificial and endogenous, sugar and non-sugar, and their roles in plants. Acquired information about their biological activities, interconversions, and metabolism improves understanding of their mechanisms of action and functions in planta. Although a number of 9-substituted cytokinins occur endogenously, many have also been prepared in laboratories to facilitate the clarification of their physiological roles and the determination of their biological properties. Here, we chart advances in knowledge of 9-substituted cytokinin conjugates from their discovery to current understanding and reciprocal interactions between biological properties and associated structural motifs. Current organic chemistry enables preparation of derivatives with better biological properties, such as improved anti-senescence, strong cell division stimulation, shoot forming, or more persistent stress tolerance compared to endogenous or canonical cytokinins. Many artificial cytokinin conjugates stimulate higher mass production than naturally occurring cytokinins, improve rooting, or simply have high stability or bioavailability. Thus, knowledge of the biosynthesis, metabolism, and activity of 9-substituted cytokinins in various plant species extends the scope for exploiting both natural and artificially prepared cytokinins in plant biotechnology, tissue culture, and agriculture.

ACS Style

Hana Vylíčilová; Magdaléna Bryksová; Vlasta Matušková; Karel Doležal; Lucie Plíhalová; Miroslav Strnad. Naturally Occurring and Artificial N9-Cytokinin Conjugates: From Synthesis to Biological Activity and Back. Biomolecules 2020, 10, 832 .

AMA Style

Hana Vylíčilová, Magdaléna Bryksová, Vlasta Matušková, Karel Doležal, Lucie Plíhalová, Miroslav Strnad. Naturally Occurring and Artificial N9-Cytokinin Conjugates: From Synthesis to Biological Activity and Back. Biomolecules. 2020; 10 (6):832.

Chicago/Turabian Style

Hana Vylíčilová; Magdaléna Bryksová; Vlasta Matušková; Karel Doležal; Lucie Plíhalová; Miroslav Strnad. 2020. "Naturally Occurring and Artificial N9-Cytokinin Conjugates: From Synthesis to Biological Activity and Back." Biomolecules 10, no. 6: 832.

Journal article
Published: 14 April 2020 in Plants
Reads 0
Downloads 0

A number of Aloe species are facing an extremely high risk of extinction due to habitat loss and over-exploitation for medicinal and ornamental trade. The last global assessment of Aloe peglerae Schönland (in 2003) ranked its global conservation status as ‘endangered’ with a decreasing population trend. In the National Red List of South African Plants, the extremely rapid decline of this species has resulted in its conservation status being elevated from ‘endangered’ to ‘critically endangered’ based on recent or new field information. This dramatic decline necessitates the development of a simple, rapid and efficient micropropagation protocol as a conservation measure. An in vitro propagation protocol was therefore established with the regeneration of 12 shoots per shoot-tip explant within 8 weeks using Murashige and Skoog (MS) medium supplemented with 2.5 µM meta-topolin riboside (an aromatic cytokinin). The rooting of the shoots with a 100% frequency on half-strength MS medium without any plant growth resulted in additional six shoots produced per cultured shoot. The resultant plantlets were successfully acclimatized with a 100% survival frequency after 6 weeks. Overall, the developed protocol can result in the production of 3906 transplantable shoots that are ready for rooting per annum from a single shoot-tip explant. It is simple and efficient for seedling production in the ex situ cultivation and conservation of the endangered A. peglerae.

ACS Style

Nontobeko A. Hlatshwayo; Stephen O. Amoo; Joshua O. Olowoyo; Karel Doležal. Efficient Micropropagation Protocol for the Conservation of the Endangered Aloe peglerae, an Ornamental and Medicinal Species. Plants 2020, 9, 506 .

AMA Style

Nontobeko A. Hlatshwayo, Stephen O. Amoo, Joshua O. Olowoyo, Karel Doležal. Efficient Micropropagation Protocol for the Conservation of the Endangered Aloe peglerae, an Ornamental and Medicinal Species. Plants. 2020; 9 (4):506.

Chicago/Turabian Style

Nontobeko A. Hlatshwayo; Stephen O. Amoo; Joshua O. Olowoyo; Karel Doležal. 2020. "Efficient Micropropagation Protocol for the Conservation of the Endangered Aloe peglerae, an Ornamental and Medicinal Species." Plants 9, no. 4: 506.

Journal article
Published: 08 December 2019 in Catalysts
Reads 0
Downloads 0

A series of titanium and vanadium complexes with oxazoline 2-(4,5-dihydro-1,3-oxazol-2-yl)phenol (L1), 2-(4-methyl-4,5-dihydro-1,3-oxazol-2-yl)phenol (L2), and oxazine 2-(5,6-dihydro-4H-1,3-oxazin-2-yl)phenol (L3) ligands were synthesized, and their structures were determined by NMR and MS methods as (L)2MtCl2. The vanadium complexes were found to be highly active in ethylene (7300 kgPE/(molV·h)) and ethylene/norbornene (5300 kgCop/(molV·h)) (co)polymerization. The polyethylene characteristics were melting temperature (123–142 °C), crystallinity degree (49–75%), molecular weight (5.7–8.5 × 105 g/mol), molecular weight distribution (1.5–2.4). The ethylene-norbornene (E-NB) copolymer characteristics were molecular weight (2.6–0.9 × 105 g/mol), molecular weight distribution (1.6–2.2), glass transition temperature (4–62 °C), norbornene incorporation (12.3–30.1 mol%) at initial concentration (0.5–1.5 mol/L). The microstructure of E-NB copolymers depends on the catalyst applied with the highest diads content for the (L3)2VCl2 and triads for the (L2)2VCl2 complexes.

ACS Style

Wioletta Ochędzan-Siodłak; Dawid Siodłak; Aleksandra Piontek; Karel Doležal. Titanium and Vanadium Catalysts with 2-Hydroxyphenyloxazoline and Oxazine Ligands for Ethylene-Norbornene (co)Polymerization. Catalysts 2019, 9, 1041 .

AMA Style

Wioletta Ochędzan-Siodłak, Dawid Siodłak, Aleksandra Piontek, Karel Doležal. Titanium and Vanadium Catalysts with 2-Hydroxyphenyloxazoline and Oxazine Ligands for Ethylene-Norbornene (co)Polymerization. Catalysts. 2019; 9 (12):1041.

Chicago/Turabian Style

Wioletta Ochędzan-Siodłak; Dawid Siodłak; Aleksandra Piontek; Karel Doležal. 2019. "Titanium and Vanadium Catalysts with 2-Hydroxyphenyloxazoline and Oxazine Ligands for Ethylene-Norbornene (co)Polymerization." Catalysts 9, no. 12: 1041.

Journal article
Published: 03 December 2019 in Antioxidants
Reads 0
Downloads 0

Eucomis autumnalis (Mill.) Chitt. subspecies autumnalis is a popular African plant that is susceptible to population decline because the bulbs are widely utilized for diverse medicinal purposes. As a result, approaches to ensure the sustainability of the plants are essential. In the current study, the influence of smoke-water (SW) and karrikinolide (KAR1 isolated from SW extract) on the phytochemicals and antioxidant activity of in vitro and greenhouse-acclimatized Eucomis autumnalis subspecies autumnalis were evaluated. Leaf explants were cultured on Murashige and Skoog (MS) media supplemented with SW (1:500, 1:1000 and 1:1500 v/v dilutions) or KAR1 (10−7, 10−8 and 10−9 M) and grown for ten weeks. In vitro regenerants were subsequently acclimatized in the greenhouse for four months. Bioactive phytochemicals in different treatments were analyzed using ultra-high performance liquid chromatography (UHPLC-MS/MS), while antioxidant potential was evaluated using two chemical tests namely: DPPH and the β-carotene model. Smoke-water and KAR1 generally influenced the quantity and types of phytochemicals in in vitro regenerants and acclimatized plants. In addition to eucomic acid, 15 phenolic acids and flavonoids were quantified; however, some were specific to either the in vitro regenerants or greenhouse-acclimatized plants. The majority of the phenolic acids and flavonoids were generally higher in in vitro regenerants than in acclimatized plants. Evidence from the chemical tests indicated an increase in antioxidant activity of SW and KAR1-treated regenerants and acclimatized plants. Overall, these findings unravel the value of SW and KAR1 as potential elicitors for bioactive phytochemicals with therapeutic activity in plants facilitated via in vitro culture systems. In addition, it affords an efficient means to ensure the sustainability of the investigated plant. Nevertheless, further studies focusing on the use of other types of antioxidant test systems (including in vivo model) and the carry-over effect of the application of SW and KAR1 for a longer duration will be pertinent. In addition, the safety of the resultant plant extracts and their pharmacological efficacy in clinical relevance systems is required.

ACS Style

Adeyemi Oladapo Aremu; Nqobile Andile Masondo; Jiri Gruz; Karel Doležal; Johannes Van Staden. Potential of Smoke-Water and One of Its Active Compounds (karrikinolide, KAR1) on the Phytochemical and Antioxidant Activity of Eucomis autumnalis. Antioxidants 2019, 8, 611 .

AMA Style

Adeyemi Oladapo Aremu, Nqobile Andile Masondo, Jiri Gruz, Karel Doležal, Johannes Van Staden. Potential of Smoke-Water and One of Its Active Compounds (karrikinolide, KAR1) on the Phytochemical and Antioxidant Activity of Eucomis autumnalis. Antioxidants. 2019; 8 (12):611.

Chicago/Turabian Style

Adeyemi Oladapo Aremu; Nqobile Andile Masondo; Jiri Gruz; Karel Doležal; Johannes Van Staden. 2019. "Potential of Smoke-Water and One of Its Active Compounds (karrikinolide, KAR1) on the Phytochemical and Antioxidant Activity of Eucomis autumnalis." Antioxidants 8, no. 12: 611.

Journal article
Published: 29 November 2019 in Bioorganic & Medicinal Chemistry
Reads 0
Downloads 0

Cytokinins are naturally occurring substances that act as plant growth regulators promoting plant growth and development, including shoot initiation and branching, and also affecting apical dominance and leaf senescence. Aromatic cytokinin 6-benzylaminopurine (BAP) has been widely used in micropropagation systems and biotechnology. However, its 9-glucoside (BAP9G) accumulates in explants, causing root inhibition and growth heterogenity. To overcome BAP disadvantages, a series of ring-substituted 2′-deoxy-9-(β)-d-ribofuranosylpurine derivatives was prepared and examined in different classical cytokinin bioassays. Amaranthus, senescence and tobacco callus bioassays were employed to provide details of cytokinin activity of 2′-deoxy-9-(β)-d-ribosides compared to their respective free bases and ribosides. The prepared derivatives were also tested for their recognition by cytokinin receptors of Arabidopsis thaliana AHK3 and CRE1/AHK4. The ability of aromatic N6-substituted adenine-2′-deoxy-9-(β)-d-ribosides to promote plant growth and delay senescence was increased considerably and, in contrast to BAP, no loss of cytokinin activity at higher concentrations was observed. The presence of a 2′-deoxyribosyl moiety at the N9-position led to an increase in cytokinin activities in comparison to the free bases and ribosides. The antioxidant capacity, cytotoxicity and effect on the MHV-68 gammaherpesvirus strain were also examined.

ACS Style

Vlasta Matušková; Marek Zatloukal; Jiří Voller; Jiří Grúz; Zuzana Pěkná; Katarína Briestenská; Jela Mistríková; Lukáš Spíchal; Karel Doležal; Miroslav Strnad. New aromatic 6-substituted 2′-deoxy-9-(β)-d-ribofuranosylpurine derivatives as potential plant growth regulators. Bioorganic & Medicinal Chemistry 2019, 28, 115230 .

AMA Style

Vlasta Matušková, Marek Zatloukal, Jiří Voller, Jiří Grúz, Zuzana Pěkná, Katarína Briestenská, Jela Mistríková, Lukáš Spíchal, Karel Doležal, Miroslav Strnad. New aromatic 6-substituted 2′-deoxy-9-(β)-d-ribofuranosylpurine derivatives as potential plant growth regulators. Bioorganic & Medicinal Chemistry. 2019; 28 (2):115230.

Chicago/Turabian Style

Vlasta Matušková; Marek Zatloukal; Jiří Voller; Jiří Grúz; Zuzana Pěkná; Katarína Briestenská; Jela Mistríková; Lukáš Spíchal; Karel Doležal; Miroslav Strnad. 2019. "New aromatic 6-substituted 2′-deoxy-9-(β)-d-ribofuranosylpurine derivatives as potential plant growth regulators." Bioorganic & Medicinal Chemistry 28, no. 2: 115230.

Original article
Published: 11 November 2019 in Molecular Diversity
Reads 0
Downloads 0

A series of benzopyrano[2,3-c]pyrazol-4(2H)-one derivatives were synthesized from readily available 1-phenyl- and 1-methyl-1H-pyrazol-3-ols by sequentially employing O-acylation, Fries rearrangement and potassium carbonate-induced cyclization. The anthelmintic properties of the obtained compounds were investigated in vivo in a model nematode, Caenorhabditis elegans. Five compounds, namely 2-phenyl[1]benzopyrano[2,3-c]pyrazol-4(2H)-one 33 and its 7-fluoro, 7-chloro-, 7-bromo- and 8-fluoro-analogues, 36, 38, 40 and 43, respectively, altered the development of C. elegans. While the activities of 33 and 43 were rather modest, compounds 36, 38 and 40 inhibited the growth of the worms at concentrations of approximately 1-3 µM. At these concentrations, the compounds did not kill the worms, but they strongly inhibited their development, with the majority of larvae never progressing past the L1 stage. Moreover, testing in non-cancer human cell lines showed that, with exception of 7-bromo derivative 40, the active compounds have favourable toxicity profiles.

ACS Style

Vaida Milišiūnaitė; Alena Kadlecová; Asta Žukauskaitė; Karel Doležal; Miroslav Strnad; Jiří Voller; Eglė Arbačiauskienė; Wolfgang Holzer; Algirdas Šačkus. Synthesis and anthelmintic activity of benzopyrano[2,3-c]pyrazol-4(2H)-one derivatives. Molecular Diversity 2019, 24, 1025 -1042.

AMA Style

Vaida Milišiūnaitė, Alena Kadlecová, Asta Žukauskaitė, Karel Doležal, Miroslav Strnad, Jiří Voller, Eglė Arbačiauskienė, Wolfgang Holzer, Algirdas Šačkus. Synthesis and anthelmintic activity of benzopyrano[2,3-c]pyrazol-4(2H)-one derivatives. Molecular Diversity. 2019; 24 (4):1025-1042.

Chicago/Turabian Style

Vaida Milišiūnaitė; Alena Kadlecová; Asta Žukauskaitė; Karel Doležal; Miroslav Strnad; Jiří Voller; Eglė Arbačiauskienė; Wolfgang Holzer; Algirdas Šačkus. 2019. "Synthesis and anthelmintic activity of benzopyrano[2,3-c]pyrazol-4(2H)-one derivatives." Molecular Diversity 24, no. 4: 1025-1042.

Preprint
Published: 24 August 2019
Reads 0
Downloads 0

The plant hormone cytokinin regulates various cell and developmental processes, including cell division and differentiation, embryogenesis, activity of shoot and root apical meristems, formation of shoot and root lateral organs and others 1. Cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via a two-component phosphorelay cascade activate transcriptional responses in the nucleus. Based on the subcellular localization of cytokinin receptors in various transient expression systems, such as tobacco leaf epidermal cells, and membrane fractionation experiments of Arabidopsis and maize, the endoplasmic reticulum (ER) membrane has been proposed as a principal hormone perception site 2–4. Intriguingly, recent study of the cytokinin transporter PUP14 has pointed out that the plasma membrane (PM)-mediated signalling might play an important role in establishment of cytokinin response gradients in various plant organs 5. However, localization of cytokinin HK receptors to the PM, although initially suggested 6, remains ambiguous. Here, by monitoring subcellular localizations of the fluorescently labelled natural cytokinin probe iP-NBD 7 and the cytokinin receptor ARABIDOPSIS HISTIDINE KINASE 4 (CRE1/AHK4) fused to GFP reporter, we show that pools of the ER-located cytokinin fluoroprobes and receptors can enter the secretory pathway and reach the PM. We demonstrate that in cells of the root apical meristem, CRE1/AHK4 localizes to the PM and the cell plate of dividing meristematic cells. Brefeldin A (BFA) experiments revealed vesicular recycling of the receptor and its accumulation in BFA compartments. Our results provide a new perspective on cytokinin signalling and the possibility of multiple sites of perception at PM and ER, which may determine specific outputs of cytokinin signalling.

ACS Style

Karolina Kubiasová; Juan Carlos Montesinos; Olga Šamajová; Jaroslav Nisler; Václav Mik; Lucie Plíhalová; Ondřej Novák; Peter Marhavý; David Zalabák; Karel Berka; Karel Doležal; Petr Galuszka; Jozef Šamaj; Miroslav Strnad; Eva Benková; Ondřej Plíhal; Lukáš Spíchal. Cytokinin fluoroprobe and receptor CRE1/AHK4 localize to both plasma membrane and endoplasmic reticulum. 2019, 744177 .

AMA Style

Karolina Kubiasová, Juan Carlos Montesinos, Olga Šamajová, Jaroslav Nisler, Václav Mik, Lucie Plíhalová, Ondřej Novák, Peter Marhavý, David Zalabák, Karel Berka, Karel Doležal, Petr Galuszka, Jozef Šamaj, Miroslav Strnad, Eva Benková, Ondřej Plíhal, Lukáš Spíchal. Cytokinin fluoroprobe and receptor CRE1/AHK4 localize to both plasma membrane and endoplasmic reticulum. . 2019; ():744177.

Chicago/Turabian Style

Karolina Kubiasová; Juan Carlos Montesinos; Olga Šamajová; Jaroslav Nisler; Václav Mik; Lucie Plíhalová; Ondřej Novák; Peter Marhavý; David Zalabák; Karel Berka; Karel Doležal; Petr Galuszka; Jozef Šamaj; Miroslav Strnad; Eva Benková; Ondřej Plíhal; Lukáš Spíchal. 2019. "Cytokinin fluoroprobe and receptor CRE1/AHK4 localize to both plasma membrane and endoplasmic reticulum." , no. : 744177.