This page has only limited features, please log in for full access.

Unclaimed
Andreas Nitsch
Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University Medicine Greifswald, Ferdinand-Sauerbruch-Straße, 17475 Greifswald, Germany

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 26 September 2020 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Background: Cold atmospheric plasma (CAP) is increasingly used in the field of oncology. Many of the mechanisms of action of CAP, such as inhibiting proliferation, DNA breakage, or the destruction of cell membrane integrity, have been investigated in many different types of tumors. In this regard, data are available from both in vivo and in vitro studies. Not only the direct treatment of a tumor but also the influence on its blood supply play a decisive role in the success of the therapy and the patient’s further prognosis. Whether the CAP influences this process is unknown, and the first indications in this regard are addressed in this study. Methods: Two different devices, kINPen and MiniJet, were used as CAP sources. Human endothelial cell line HDMEC were treated directly and indirectly with CAP, and growth kinetics were performed. To indicate apoptotic processes, caspase-3/7 assay and TUNEL assay were used. The influence of CAP on cellular metabolism was examined using the MTT and glucose assay. After CAP exposure, tube formation assay was performed to examine the capillary tube formation abilities of HDMEC and their migration was messured in separate assays. To investigate in a possible mutagenic effect of CAP treatment, a hypoxanthine-guanine-phosphoribosyl-transferase assay with non malignant cell (CCL-93) line was performed. Results: The direct CAP treatment of the HDMEC showed a robust growth-inhibiting effect, but the indirect one did not. The MMT assay showed an apparent reduction in cell metabolism in the first 24 h after CAP treatment, which appeared to normalize 48 h and 72 h after CAP application. These results were also confirmed by the glucose assay. The caspase 3/7 assay and TUNEL assay showed a significant increase in apoptotic processes in the HDMEC after CAP treatment. These results were independent of the CAP device. Both the migration and tube formation of HDMEC were significant inhibited after CAP-treatment. No malignant effects could be demonstrated by the CAP treatment on a non-malignant cell line.

ACS Style

Lyubomir Haralambiev; Ole Neuffer; Andreas Nitsch; Nele C. Kross; Sander Bekeschus; Peter Hinz; Alexander Mustea; Axel Ekkernkamp; Denis Gümbel; Matthias B. Stope. Inhibition of Angiogenesis by Treatment with Cold Atmospheric Plasma as a Promising Therapeutic Approach in Oncology. International Journal of Molecular Sciences 2020, 21, 7098 .

AMA Style

Lyubomir Haralambiev, Ole Neuffer, Andreas Nitsch, Nele C. Kross, Sander Bekeschus, Peter Hinz, Alexander Mustea, Axel Ekkernkamp, Denis Gümbel, Matthias B. Stope. Inhibition of Angiogenesis by Treatment with Cold Atmospheric Plasma as a Promising Therapeutic Approach in Oncology. International Journal of Molecular Sciences. 2020; 21 (19):7098.

Chicago/Turabian Style

Lyubomir Haralambiev; Ole Neuffer; Andreas Nitsch; Nele C. Kross; Sander Bekeschus; Peter Hinz; Alexander Mustea; Axel Ekkernkamp; Denis Gümbel; Matthias B. Stope. 2020. "Inhibition of Angiogenesis by Treatment with Cold Atmospheric Plasma as a Promising Therapeutic Approach in Oncology." International Journal of Molecular Sciences 21, no. 19: 7098.

Journal article
Published: 23 June 2020 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Osteosarcoma and Ewing’s sarcoma are the most common malignant bone tumors. Conventional therapies such as polychemotherapy, local surgery, and radiotherapy improve the clinical outcome for patients. However, they are accompanied by acute and chronic side effects that affect the quality of life of patients, motivating novel research lines on therapeutic options for the treatment of sarcomas. Previous experimental work with physical plasma operated at body temperature (cold atmospheric plasma, CAP) demonstrated anti-oncogenic effects on different cancer cell types. This study investigated the anti-cancer effect of CAP on two bone sarcoma entities, osteosarcoma and Ewing’s sarcoma, which were represented by four cell lines (U2-OS, MNNG/HOS, A673, and RD-ES). A time-dependent anti-proliferative effect of CAP on all cell lines was observed. CAP-induced alterations in cell membrane functionality were detected by performing a fluorescein diacetate (FDA) release assay and an ATP release assay. Additionally, modifications of the cell membrane and modifications in the actin cytoskeleton composition were examined using fluorescence microscopy monitoring dextran-uptake assay and G-/F-actin distribution. Furthermore, the CAP-induced induction of apoptosis was determined by TUNEL and active caspases assays. The observations suggest that a single CAP treatment of bone sarcoma cells may have significant anti-oncogenic effects and thus may be a promising extension to existing applications.

ACS Style

Josephine M. Jacoby; Silas Strakeljahn; Andreas Nitsch; Sander Bekeschus; Peter Hinz; Alexander Mustea; Axel Ekkernkamp; Mladen V. Tzvetkov; Lyubomir Haralambiev; Matthias B. Stope. An Innovative Therapeutic Option for the Treatment of Skeletal Sarcomas: Elimination of Osteo- and Ewing’s Sarcoma Cells Using Physical Gas Plasma. International Journal of Molecular Sciences 2020, 21, 4460 .

AMA Style

Josephine M. Jacoby, Silas Strakeljahn, Andreas Nitsch, Sander Bekeschus, Peter Hinz, Alexander Mustea, Axel Ekkernkamp, Mladen V. Tzvetkov, Lyubomir Haralambiev, Matthias B. Stope. An Innovative Therapeutic Option for the Treatment of Skeletal Sarcomas: Elimination of Osteo- and Ewing’s Sarcoma Cells Using Physical Gas Plasma. International Journal of Molecular Sciences. 2020; 21 (12):4460.

Chicago/Turabian Style

Josephine M. Jacoby; Silas Strakeljahn; Andreas Nitsch; Sander Bekeschus; Peter Hinz; Alexander Mustea; Axel Ekkernkamp; Mladen V. Tzvetkov; Lyubomir Haralambiev; Matthias B. Stope. 2020. "An Innovative Therapeutic Option for the Treatment of Skeletal Sarcomas: Elimination of Osteo- and Ewing’s Sarcoma Cells Using Physical Gas Plasma." International Journal of Molecular Sciences 21, no. 12: 4460.

Journal article
Published: 26 March 2020 in International Journal of Molecular Sciences
Reads 0
Downloads 0

Chondrosarcoma is the second most common malign bone tumor in adults. Surgical resection of the tumor is recommended because of its resistance to clinical treatment such as chemotherapy and radiation therapy. Thus, the prognosis for patients mainly depends on sufficient surgical resection. Due to this, research on alternative therapies is needed. Cold atmospheric plasma (CAP) is an ionized gas that contains various reactive species. Previous studies have shown an anti-oncogenic potential of CAP on different cancer cell types. The current study examined the effects of treatment with CAP on two chondrosarcoma cell lines (CAL-78, SW1353). Through proliferation assay, the cell growth after CAP-treatment was determined. A strong antiproliferative effect for both cell lines was detected. By fluorescein diacetate (FDA) assay and ATP release assay, alterations in the cell membrane and associated translocation of low molecular weight particles through the cytoplasmic membrane were observed. In supernatant, the non-membrane-permeable FDA and endogenously synthesized ATP detected suggest an increased membrane permeability after CAP treatment. Similar results were shown by the dextran-uptake assay. Furthermore, fluorescence microscopic G-/F-actin assay was performed. G- and F-actin were selectively dyed, and the ratio was measured. The presented results indicate CAP-induced changes in cell membrane function and possible alterations in actin-cytoskeleton, which may contribute to the antiproliferative effects of CAP.

ACS Style

Lyubomir Haralambiev; Andreas Nitsch; Josephine M. Jacoby; Silas Strakeljahn; Sander Bekeschus; Alexander Mustea; Axel Ekkernkamp; Matthias B. Stope. Cold Atmospheric Plasma Treatment of Chondrosarcoma Cells Affects Proliferation and Cell Membrane Permeability. International Journal of Molecular Sciences 2020, 21, 2291 .

AMA Style

Lyubomir Haralambiev, Andreas Nitsch, Josephine M. Jacoby, Silas Strakeljahn, Sander Bekeschus, Alexander Mustea, Axel Ekkernkamp, Matthias B. Stope. Cold Atmospheric Plasma Treatment of Chondrosarcoma Cells Affects Proliferation and Cell Membrane Permeability. International Journal of Molecular Sciences. 2020; 21 (7):2291.

Chicago/Turabian Style

Lyubomir Haralambiev; Andreas Nitsch; Josephine M. Jacoby; Silas Strakeljahn; Sander Bekeschus; Alexander Mustea; Axel Ekkernkamp; Matthias B. Stope. 2020. "Cold Atmospheric Plasma Treatment of Chondrosarcoma Cells Affects Proliferation and Cell Membrane Permeability." International Journal of Molecular Sciences 21, no. 7: 2291.

Journal article
Published: 03 February 2020 in Anticancer Research
Reads 0
Downloads 0

Cold atmospheric plasma (CAP) has a variety of anticancer effects on different cancer cell types. In osteosarcoma (OS) cells, CAP reduces growth and motility, induces apoptosis, and alters secretion of cellular factors. The influence of CAP on membrane integrity of OS cells is unknown. Two different OS cell lines (U-2 OS and MNNG-HOS) were treated with CAP. Proliferation assays for cell growth after treatment was performed. Alterations in membrane permeability and the associated translocation of low molecular weight particles through the cytoplasmic membrane of OS cells after CAP treatment were shown in fluorescein diacetate (FDA) assays. FDA increasingly passed the membrane after CAP treatment and this effect depended on the duration of treatment. It was also shown that after CAP treatment, FDA was able to diffuse into the cells from the outside as well as out of the cell interior. These effects were observed when CAP-treated buffer was used and therefore no direct contact between cells and CAP occurred. The observations suggest that changes in membrane permeability and function may contribute to the antiproliferative effects of CAP.

ACS Style

Lyubomir Haralambiev; Andreas Nitsch; Rebekka Einenkel; Damián Muzzio; Nadine Gelbrich; Martin Burchardt; Marek Zygmunt; Axel Ekkernkamp; Matthias B. Stope; Denis Gümbel. The Effect of Cold Atmospheric Plasma on the Membrane Permeability of Human Osteosarcoma Cells. Anticancer Research 2020, 40, 841 -846.

AMA Style

Lyubomir Haralambiev, Andreas Nitsch, Rebekka Einenkel, Damián Muzzio, Nadine Gelbrich, Martin Burchardt, Marek Zygmunt, Axel Ekkernkamp, Matthias B. Stope, Denis Gümbel. The Effect of Cold Atmospheric Plasma on the Membrane Permeability of Human Osteosarcoma Cells. Anticancer Research. 2020; 40 (2):841-846.

Chicago/Turabian Style

Lyubomir Haralambiev; Andreas Nitsch; Rebekka Einenkel; Damián Muzzio; Nadine Gelbrich; Martin Burchardt; Marek Zygmunt; Axel Ekkernkamp; Matthias B. Stope; Denis Gümbel. 2020. "The Effect of Cold Atmospheric Plasma on the Membrane Permeability of Human Osteosarcoma Cells." Anticancer Research 40, no. 2: 841-846.

Journal article
Published: 29 October 2019 in In Vivo
Reads 0
Downloads 0

Background/Aim: The structural integrity of the eukaryotic cytoplasmic membrane is of crucial importance for its cell biological function and thus for the survival of the cell. Physical and chemical noxae can interact in various ways with components of the cytoplasmic membrane, influence its permeability and thus mediate toxic effects. In the study presented, changes in membrane permeability were quantified by intracellular accumulation of a fluorescent dye and by the release of the fluorescent dye from dye-loaded cells. Materials and Methods: Non-malignant (RC-124) and malignant (786-O, Caki-1) renal cells were permeabilized with different concentrations of Triton X-100. The permeability of the membrane was determined at the single-cell level by the uptake of the dye into the cell inner by flow cytometry. In addition, a fluorescence plate reader was used to detect and quantify the release of the dye into the cell culture supernatant. Results: Both malignant and non-malignant cells showed a dose-dependent alteration of membrane permeability after treatment with Triton X-100. In the presence of the fluorescent dye, significantly more dye was introduced into the permeabilized cells compared to control incubations. Vice versa, Triton X-100-treated and dye-loaded cells released significantly more dye into the cell culture supernatant. Conclusion: The combination of measurement of intracellular accumulated and extracellular released dye can quantifiably detect changes in membrane permeability due to cell-membrane damage. The combination of two different measurement methods offers additional value in reliable detection of membrane-damaging, potentially toxic influences.

ACS Style

Andreas Nitsch; Lyubomir Haralambiev; Rebekka Einenkel; Damián Muzzio; Marek T. Zygmunt; Axel Ekkernkamp; Martin Burchardt; Matthias B. Stope. Determination of In Vitro Membrane Permeability by Analysis of Intracellular and Extracellular Fluorescein Signals in Renal Cells. In Vivo 2019, 33, 1767 -1771.

AMA Style

Andreas Nitsch, Lyubomir Haralambiev, Rebekka Einenkel, Damián Muzzio, Marek T. Zygmunt, Axel Ekkernkamp, Martin Burchardt, Matthias B. Stope. Determination of In Vitro Membrane Permeability by Analysis of Intracellular and Extracellular Fluorescein Signals in Renal Cells. In Vivo. 2019; 33 (6):1767-1771.

Chicago/Turabian Style

Andreas Nitsch; Lyubomir Haralambiev; Rebekka Einenkel; Damián Muzzio; Marek T. Zygmunt; Axel Ekkernkamp; Martin Burchardt; Matthias B. Stope. 2019. "Determination of In Vitro Membrane Permeability by Analysis of Intracellular and Extracellular Fluorescein Signals in Renal Cells." In Vivo 33, no. 6: 1767-1771.

Journal article
Published: 20 February 2019 in Toxins
Reads 0
Downloads 0

Interaction of Staphylococcus aureus alpha-toxin (hemolysin A, Hla) with eukaryotic cell membranes is mediated by proteinaceous receptors and certain lipid domains in host cell plasma membranes. Hla is secreted as a 33 kDa monomer that forms heptameric transmembrane pores whose action compromises maintenance of cell shape and epithelial tightness. It is not exactly known whether certain membrane lipid domains of host cells facilitate adhesion of Ha monomers, oligomerization, or pore formation. We used sphingomyelinase (hemolysin B, Hlb) expressed by some strains of staphylococci to pre-treat airway epithelial model cells in order to specifically decrease the sphingomyelin (SM) abundance in their plasma membranes. Such a pre-incubation exclusively removed SM from the plasma membrane lipid fraction. It abrogated the formation of heptamers and prevented the formation of functional transmembrane pores. Hla exposure of rHlb pre-treated cells did not result in increases in [Ca2+]i, did not induce any microscopically visible changes in cell shape or formation of paracellular gaps, and did not induce hypo-phosphorylation of the actin depolymerizing factor cofilin as usual. Removal of sphingomyelin from the plasma membranes of human airway epithelial cells completely abrogates the deleterious actions of Staphylococcus aureus alpha-toxin.

ACS Style

Sabine Ziesemer; Nils Möller; Andreas Nitsch; Christian Müller; Achim G. Beule; Jan-Peter Hildebrandt. Sphingomyelin Depletion from Plasma Membranes of Human Airway Epithelial Cells Completely Abrogates the Deleterious Actions of S. aureus Alpha-Toxin. Toxins 2019, 11, 126 .

AMA Style

Sabine Ziesemer, Nils Möller, Andreas Nitsch, Christian Müller, Achim G. Beule, Jan-Peter Hildebrandt. Sphingomyelin Depletion from Plasma Membranes of Human Airway Epithelial Cells Completely Abrogates the Deleterious Actions of S. aureus Alpha-Toxin. Toxins. 2019; 11 (2):126.

Chicago/Turabian Style

Sabine Ziesemer; Nils Möller; Andreas Nitsch; Christian Müller; Achim G. Beule; Jan-Peter Hildebrandt. 2019. "Sphingomyelin Depletion from Plasma Membranes of Human Airway Epithelial Cells Completely Abrogates the Deleterious Actions of S. aureus Alpha-Toxin." Toxins 11, no. 2: 126.