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Regeneration of body parts and their interaction with the immune response is a poorly understood aspect of earthworm biology. Consequently, we aimed to study the mechanisms of innate immunity during regeneration in Eisenia andrei earthworms. In the course of anterior and posterior regeneration, we documented the kinetical aspects of segment restoration by histochemistry. Cell proliferation peaked at two weeks and remitted by four weeks in regenerating earthworms. Apoptotic cells were present throughout the cell renewal period. Distinct immune cell (e.g., coelomocyte) subsets were accumulated in the newly-formed blastema in the close proximity of the apoptotic area. Regenerating earthworms have decreased pattern recognition receptors (PRRs) (e.g., TLR, except for scavenger receptor) and antimicrobial peptides (AMPs) (e.g., lysenin) mRNA patterns compared to intact earthworms. In contrast, at the protein level, mirroring regulation of lysenins became evident. Experimental coelomocyte depletion caused significantly impaired cell divisions and blastema formation during anterior and posterior regeneration. These obtained novel data allow us to gain insight into the intricate interactions of regeneration and invertebrate innate immunity.
Kornélia Bodó; Zoltán Kellermayer; Zoltán László; Ákos Boros; Bohdana Kokhanyuk; Péter Németh; Péter Engelmann. Injury-Induced Innate Immune Response During Segment Regeneration of the Earthworm, Eisenia andrei. International Journal of Molecular Sciences 2021, 22, 2363 .
AMA StyleKornélia Bodó, Zoltán Kellermayer, Zoltán László, Ákos Boros, Bohdana Kokhanyuk, Péter Németh, Péter Engelmann. Injury-Induced Innate Immune Response During Segment Regeneration of the Earthworm, Eisenia andrei. International Journal of Molecular Sciences. 2021; 22 (5):2363.
Chicago/Turabian StyleKornélia Bodó; Zoltán Kellermayer; Zoltán László; Ákos Boros; Bohdana Kokhanyuk; Péter Németh; Péter Engelmann. 2021. "Injury-Induced Innate Immune Response During Segment Regeneration of the Earthworm, Eisenia andrei." International Journal of Molecular Sciences 22, no. 5: 2363.
Most picornaviruses of the family Picornaviridae are relatively well known, but there are certain “neglected” genera like Bopivirus, containing a single uncharacterised sequence (bopivirus A1, KM589358) with very limited background information. In this study, three novel picornaviruses provisionally called ovipi-, gopi- and bopivirus/Hun (MW298057-MW298059) from enteric samples of asymptomatic ovine, caprine and bovine respectively, were determined using RT-PCR and dye-terminator sequencing techniques. These monophyletic viruses share the same type II-like IRES, NPGP-type 2A, similar genome layout (4-3-4) and cre-localisations. Culture attempts of the study viruses, using six different cell lines, yielded no evidence of viral growth in vitro. Genomic and phylogenetic analyses show that bopivirus/Hun of bovine belongs to the species Bopivirus A, while the closely related ovine-origin ovipi- and caprine-origin gopivirus could belong to a novel species “Bopivirus B” in the genus Bopivirus. Epidemiological investigation of N = 269 faecal samples of livestock (ovine, caprine, bovine, swine and rabbit) from different farms in Hungary showed that bopiviruses were most prevalent among <12-month-old ovine, caprine and bovine, but undetectable in swine and rabbit. VP1 capsid-based phylogenetic analyses revealed the presence of multiple lineages/genotypes, including closely related ovine/caprine strains, suggesting the possibility of ovine–caprine interspecies transmission of certain bopiviruses.
Zoltán László; Péter Pankovics; Gábor Reuter; Attila Cságola; Ádám Bálint; Mihály Albert; Ákos Boros. Multiple Types of Novel Enteric Bopiviruses (Picornaviridae) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary. Viruses 2021, 13, 66 .
AMA StyleZoltán László, Péter Pankovics, Gábor Reuter, Attila Cságola, Ádám Bálint, Mihály Albert, Ákos Boros. Multiple Types of Novel Enteric Bopiviruses (Picornaviridae) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary. Viruses. 2021; 13 (1):66.
Chicago/Turabian StyleZoltán László; Péter Pankovics; Gábor Reuter; Attila Cságola; Ádám Bálint; Mihály Albert; Ákos Boros. 2021. "Multiple Types of Novel Enteric Bopiviruses (Picornaviridae) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary." Viruses 13, no. 1: 66.
Hepatitis E virus (HEV) (family Hepeviridae) is one of the most common human pathogens, causing acute hepatitis and an increasingly recognized etiological agent in chronic hepatitis and extrahepatic manifestations. Recent studies reported that not only are the classical members of the species Orthohepevirus A (HEV-A) pathogenic to humans but a genetically highly divergent rat origin hepevirus (HEV-C1) in species Orthohepevirus C (HEV-C) is also able to cause zoonotic infection and symptomatic disease (hepatitis) in humans. This review summarizes the current knowledge of hepeviruses in rodents with special focus of rat origin HEV-C1. Cross-species transmission and genetic diversity of HEV-C1 and confirmation of HEV-C1 infections and symptomatic disease in humans re-opened the long-lasting and full of surprises story of HEV in human. This novel knowledge has a consequence to the epidemiology, clinical aspects, laboratory diagnosis, and prevention of HEV infection in humans.
Gábor Reuter; Ákos Boros; Péter Pankovics. Review of Hepatitis E Virus in Rats: Evident Risk of Species Orthohepevirus C to Human Zoonotic Infection and Disease. Viruses 2020, 12, 1148 .
AMA StyleGábor Reuter, Ákos Boros, Péter Pankovics. Review of Hepatitis E Virus in Rats: Evident Risk of Species Orthohepevirus C to Human Zoonotic Infection and Disease. Viruses. 2020; 12 (10):1148.
Chicago/Turabian StyleGábor Reuter; Ákos Boros; Péter Pankovics. 2020. "Review of Hepatitis E Virus in Rats: Evident Risk of Species Orthohepevirus C to Human Zoonotic Infection and Disease." Viruses 12, no. 10: 1148.
All of the known porcine sapeloviruses (PSVs) currently belong to a single genotype in the genus Sapelovirus (family Picornaviridae). Here, the complete genome of a second, possibly recombinant, genotype of PSV strain SZ1M-F/PSV/HUN2013 (MN807752) from a faecal sample of a paraplegic pig in Hungary was characterized using viral metagenomics and RT-PCR. This sapelovirus strain showed only 64 % nucleotide identity in the VP1 region to its closest PSV-1 relative. Complete VP1 sequence-based epidemiological investigations of PSVs circulating in Hungary showed the presence of diverse strains found in high prevalence in enteric and respiratory samples collected from both asymptomatic and paraplegic pigs from 12 swine farms. Virus isolation attempts using PK-15 cell cultures were successful in 3/8 cases for the classic but not the novel PSV genotype. Sequence comparisons of faeces and isolate strains derived VP1 showed that cultured PSV strains not always represent the dominant PSVs found in vivo.
Ákos Boros; Zoltán László; Péter Pankovics; András Marosi; Mihály Albert; Attila Cságola; Hunor Bíró; Elizabeth Fahsbender; Eric Delwart; Gábor Reuter. High prevalence, genetic diversity and a potentially novel genotype of Sapelovirus A (Picornaviridae) in enteric and respiratory samples in Hungarian swine farms. Journal of General Virology 2020, 101, 609 -621.
AMA StyleÁkos Boros, Zoltán László, Péter Pankovics, András Marosi, Mihály Albert, Attila Cságola, Hunor Bíró, Elizabeth Fahsbender, Eric Delwart, Gábor Reuter. High prevalence, genetic diversity and a potentially novel genotype of Sapelovirus A (Picornaviridae) in enteric and respiratory samples in Hungarian swine farms. Journal of General Virology. 2020; 101 (6):609-621.
Chicago/Turabian StyleÁkos Boros; Zoltán László; Péter Pankovics; András Marosi; Mihály Albert; Attila Cságola; Hunor Bíró; Elizabeth Fahsbender; Eric Delwart; Gábor Reuter. 2020. "High prevalence, genetic diversity and a potentially novel genotype of Sapelovirus A (Picornaviridae) in enteric and respiratory samples in Hungarian swine farms." Journal of General Virology 101, no. 6: 609-621.
Zebrafish have been extensively used as a model system for research in vertebrate development and pathogen-host interactions. We describe the complete genome of a novel picornavirus identified during a viral metagenomics analysis of zebrafish gut tissue. The closest relatives of this virus showed identity of <20% in their P1 capsids and <36% in their RdRp qualifying zebrafish picornavirus-1 (ZfPV-1) as member of a novel genus with a proposed name of Cyprivirus. Reverse transcription (RT)-PCR testing of zebrafish from North America, Europe, and Asia showed ZfPV-1 to be globally distributed, being detected in 23 of 41 (56%) institutions tested. In situ hybridization of whole zebrafish showed viral RNA was restricted to a subset of enterocytes and cells in the subjacent lamina propria of the intestine and the intestinal mucosa. This naturally occurring and apparently asymptomatic infection (in wild-type zebrafish lineage AB) provides a natural infection system to study picornavirus-host interactions in an advanced vertebrate model organism. Whether ZfPV-1 infection affects any immunological, developmental, or other biological processes in wild-type or mutant zebrafish lineages remains to be determined.
Eda Altan; Steven V. Kubiski; Akos Boros; Gábor Reuter; Mohammadreza Sadeghi; Xutao Deng; Erica K. Creighton; Marcus J. Crim; Eric Delwart. A Highly Divergent Picornavirus Infecting the Gut Epithelia of Zebrafish (Danio rerio) in Research Institutions Worldwide. Zebrafish 2019, 16, 291 -299.
AMA StyleEda Altan, Steven V. Kubiski, Akos Boros, Gábor Reuter, Mohammadreza Sadeghi, Xutao Deng, Erica K. Creighton, Marcus J. Crim, Eric Delwart. A Highly Divergent Picornavirus Infecting the Gut Epithelia of Zebrafish (Danio rerio) in Research Institutions Worldwide. Zebrafish. 2019; 16 (3):291-299.
Chicago/Turabian StyleEda Altan; Steven V. Kubiski; Akos Boros; Gábor Reuter; Mohammadreza Sadeghi; Xutao Deng; Erica K. Creighton; Marcus J. Crim; Eric Delwart. 2019. "A Highly Divergent Picornavirus Infecting the Gut Epithelia of Zebrafish (Danio rerio) in Research Institutions Worldwide." Zebrafish 16, no. 3: 291-299.
Astroviruses (family Astroviridae) and hepeviruses (family Hepeviridae) are small, non-enveloped viruses with genetically diverse +ssRNA genome thought to be enteric pathogens infecting vertebrates including humans. Recently, many novel astro- and hepatitis E virus-like +ssRNA viruses have been described from lower vertebrate species. The non-structural proteins of astro- and hepeviruses are highly diverse, but the structural/capsid proteins represent a common phylogenetic position shed the light of their common origin by inter-viral recombination. In this study, a novel astrovirus/hepevirus-like virus with +ssRNA genome (Er/SZAL5/HUN/2011, MK450332) was serendipitously identified and characterized from 3 (8.5%) out of 35 European roller (Coracias garrulus) faecal samples by RT-PCR in Hungary. The complete genome of Er/SZAL5/HUN/2011 (MK450332) is 8402 nt-long and potentially composed three non-overlapping open reading frames (ORFs): ORF1a (4449 nt/1482aa), ORF1b (1206 nt/401aa) and ORF2 (1491 nt/496aa). The ORF1ab has an astrovirus-like genome organization containing the non-structural conserved elements (TM, CC, NLS, VPg) and enzyme residues (trypsine-like protease, RNA-dependent RNA-polymerase) with low amino acid sequence identity, 15% (ORF1a) and 44% (ORF1b), to astroviruses. Supposedly the ORF2 is a capsid protein but neither the astrovirus-like subgenomic RNA promoter (sgRNA) nor the astrovirus-like capsid characteristics have been identifiable. However, the predicted capsid protein (ORF2) showed 26% identity to the corresponding protein of hepevirus-like novel Rana hepevirus (MH330682). This novel +ssRNA virus strain Er/SZAL5/HUN/2011 with astrovirus-like genome organization in the non-structural genome regions (ORF1a and ORF1b) and Rana hepevirus-related capsid (ORF2) protein represent a potentially recombinant virus species and supports the common origin hypothesis, although, the taxonomic position of the studied virus is still under discussion.
Péter Pankovics; Akos Boros; Tamás Kiss; Peter Engelmann; Gábor Reuter. Genetically highly divergent RNA virus with astrovirus-like (5′-end) and hepevirus-like (3′-end) genome organization in carnivorous birds, European roller (Coracias garrulus). Infection, Genetics and Evolution 2019, 71, 215 -223.
AMA StylePéter Pankovics, Akos Boros, Tamás Kiss, Peter Engelmann, Gábor Reuter. Genetically highly divergent RNA virus with astrovirus-like (5′-end) and hepevirus-like (3′-end) genome organization in carnivorous birds, European roller (Coracias garrulus). Infection, Genetics and Evolution. 2019; 71 ():215-223.
Chicago/Turabian StylePéter Pankovics; Akos Boros; Tamás Kiss; Peter Engelmann; Gábor Reuter. 2019. "Genetically highly divergent RNA virus with astrovirus-like (5′-end) and hepevirus-like (3′-end) genome organization in carnivorous birds, European roller (Coracias garrulus)." Infection, Genetics and Evolution 71, no. : 215-223.
Most human parechovirus (HPeV, family Picornaviridae) infections are asymptomatic but may cause gastroenteritis in children. New reports show that HPeVs can be associated with severe central nervous system symptoms and sepsis-like syndromes in infants. The clinical significance of HPeVs in Hungary has not been investigated before. The aim of this study was to detect genotype HPeV in faecal samples of children and analysis of the clinical symptoms. For the detection and genotyping of HPeV strains, reverse transcription-polymerase chain reaction and sequencing methods were used from faecal samples of children with gastroenteritis divided into three groups: group A) hospitalised children younger than 10 years (n = 75); group B) 0-12 months infants (n = 237) and group C) children less than 18 years of age with sepsis-like/neurological symptoms (n = 105) were tested. Three HPeV positive samples (3/75, 4%) were found in group A, two of them belong to the HPeV type 1, the third was non-typeable. All positive samples were from infants of 7 to 11 months of age. In group B, HPeV was detected in 6.8% (16/237) of the samples. Five were HPeV1, six were HPeV3 and five were non-typeable. While most of the infants with HPeV1 (4/5) did not require hospitalisation, 83% of the HPeV3 infected infants (5/6) did. Five (4.8%) HPeV strains detected from children less than 18 years of age with sepsis-like/neurological symptoms (group C) belonged to HPeV1 (three) and HPeV3 (two). All positive samples were from hospitalised infants less than 2 months of age. HPeV1 infections are less severe in infants than HPeV3 infections. The leading symptom of HPeV1 was diarrhoea, although in infants less than 1-2 months neurological symptoms (somnolence, lassitude) were also present. HPeV3 infections were more common among newborns. The main symptoms of severe HPeV3 infection are: gastroenteritis (7/8), fever ≥38 °C (6/7), loss of appetite (6/7), rash (4/7), somnolence/lassitude (3/7), sepsis-like syndrome (3/7) and respiratory symptoms (2/7). Orv Hetil. 2019; 160(10): 386-395.
Akos Boros; Zsófia Hamarics; Hajnalka Fenyvesi; Zoltán Liptai; Zoltán Nyul; Péter Pankovics; Gábor Reuter. A humán parechovírusok klinikai jelentősége súlyos újszülött- és csecsemőkori fertőzésekben hazánkban. Orvosi Hetilap 2019, 160, 386 -395.
AMA StyleAkos Boros, Zsófia Hamarics, Hajnalka Fenyvesi, Zoltán Liptai, Zoltán Nyul, Péter Pankovics, Gábor Reuter. A humán parechovírusok klinikai jelentősége súlyos újszülött- és csecsemőkori fertőzésekben hazánkban. Orvosi Hetilap. 2019; 160 (10):386-395.
Chicago/Turabian StyleAkos Boros; Zsófia Hamarics; Hajnalka Fenyvesi; Zoltán Liptai; Zoltán Nyul; Péter Pankovics; Gábor Reuter. 2019. "A humán parechovírusok klinikai jelentősége súlyos újszülött- és csecsemőkori fertőzésekben hazánkban." Orvosi Hetilap 160, no. 10: 386-395.
Zebrafish have been extensively used as a model system for research in vertebrate development and pathogen-host interactions. We describe the complete genome of a novel picornavirus identified during a viral metagenomics analysis of zebrafish gut tissue. The closest relatives of this virus showed identity of ≤19.8% in their P1 capsids and ≤35.4% in their RdRp qualifying zebrafish picornavirus 1 (ZfPV1) as member of a novel genus with a proposed name of Cyprivirus. RT-PCR testing of zebrafish from 41 institutions from North America, Europe, and Asia showed ZfPV1 to be highly prevalent world-wide. In situ hybridization of whole zebrafish showed viral RNA was restricted to a subset of enterocytes and cells in the subjacent lamina propria of the intestine and the intestinal mucosa. This naturally occurring and apparently asymptomatic infection (in wild type zebrafish lineage AB) provides a natural infection system to study picornavirus-host interactions in an advanced vertebrate model organism. Whether ZfPV1 infection affects any immunological, developmental or other biological processes in wild type or mutant zebrafish lineages remains to be determined.
Eda Altan; Steven V. Kubiski; Akos Boros; Gabor Reuter; Mohammadreza Sadeghi; Xutao Deng; Erica K. Creighton; Marcus J. Crim; Eric Delwart. A highly divergent picornavirus infecting the gut epithelia of zebrafish (Danio rerio) in research institutions world-wide. 2018, 463083 .
AMA StyleEda Altan, Steven V. Kubiski, Akos Boros, Gabor Reuter, Mohammadreza Sadeghi, Xutao Deng, Erica K. Creighton, Marcus J. Crim, Eric Delwart. A highly divergent picornavirus infecting the gut epithelia of zebrafish (Danio rerio) in research institutions world-wide. . 2018; ():463083.
Chicago/Turabian StyleEda Altan; Steven V. Kubiski; Akos Boros; Gabor Reuter; Mohammadreza Sadeghi; Xutao Deng; Erica K. Creighton; Marcus J. Crim; Eric Delwart. 2018. "A highly divergent picornavirus infecting the gut epithelia of zebrafish (Danio rerio) in research institutions world-wide." , no. : 463083.
Astroviruses are thought to be enteric pathogens. Since 2010, a certain group of astroviruses has increasingly been recognized, using up-to-date random amplification and high-throughput next-generation sequencing (NGS) methods, as potential neurovirulent (Ni) pathogens of severe central nervous system (CNS) infections, causing encephalitis, meningoencephalitis, and meningoencephalomyelitis.
Gábor Reuter; Péter Pankovics; Ákos Boros. Nonsuppurative (Aseptic) Meningoencephalomyelitis Associated with Neurovirulent Astrovirus Infections in Humans and Animals. Clinical Microbiology Reviews 2018, 31, e00040-18 .
AMA StyleGábor Reuter, Péter Pankovics, Ákos Boros. Nonsuppurative (Aseptic) Meningoencephalomyelitis Associated with Neurovirulent Astrovirus Infections in Humans and Animals. Clinical Microbiology Reviews. 2018; 31 (4):e00040-18.
Chicago/Turabian StyleGábor Reuter; Péter Pankovics; Ákos Boros. 2018. "Nonsuppurative (Aseptic) Meningoencephalomyelitis Associated with Neurovirulent Astrovirus Infections in Humans and Animals." Clinical Microbiology Reviews 31, no. 4: e00040-18.
Picobirnaviruses (PBVs) of family Picobirnaviridae have bisegmented (S1 and S2 segments), double-stranded RNA genomes. In this study a total of N = 12 complete chicken PBVs (ChPBV) segments (N = 5 of S1 and N = 7 of S2, Acc. Nos.: MH425579-90) were determined using viral metagenomic and RT-PCR techniques from a single cloacal sample of a diarrheic chicken. The identified ChPBV segments are unrelated to each other and distant from all of the currently known PBVs. In silico sequence analyses revealed the presence of conserved prokaryotic Shine-Dalgarno-like (SD-like) sequences upstream of the three presumed open reading frames (ORFs) of the S1 and a single presumed ORF of the S2 segments. According to the results of expression analyses in E. coli using 6xHis-tagged recombinant ChPBV segment 1 construct and Western blot these SD-like sequences are functional in vivo suggesting that S1 of study PBVs can contain three ORFs and supporting the bacteriophage-nature of PBVs.
Akos Boros; Beáta Polgár; Péter Pankovics; Hajnalka Fenyvesi; Peter Engelmann; Tung Phan; Eric Delwart; Gábor Reuter. Multiple divergent picobirnaviruses with functional prokaryotic Shine-Dalgarno ribosome binding sites present in cloacal sample of a diarrheic chicken. Virology 2018, 525, 62 -72.
AMA StyleAkos Boros, Beáta Polgár, Péter Pankovics, Hajnalka Fenyvesi, Peter Engelmann, Tung Phan, Eric Delwart, Gábor Reuter. Multiple divergent picobirnaviruses with functional prokaryotic Shine-Dalgarno ribosome binding sites present in cloacal sample of a diarrheic chicken. Virology. 2018; 525 ():62-72.
Chicago/Turabian StyleAkos Boros; Beáta Polgár; Péter Pankovics; Hajnalka Fenyvesi; Peter Engelmann; Tung Phan; Eric Delwart; Gábor Reuter. 2018. "Multiple divergent picobirnaviruses with functional prokaryotic Shine-Dalgarno ribosome binding sites present in cloacal sample of a diarrheic chicken." Virology 525, no. : 62-72.
Picobirnaviruses (PBVs) are bisegmented viruses with a wide geographical and host species distribution. The number of novel PBV sequences has been increasing with the help of the viral metagenomics. A novel picobirnavirus strain, pbv/CHK/M3841/HUN/2011, was identified by viral metagenomics; the complete segment 1 (MH327933) and 2 (MH327934) sequences were obtained by RT-PCR from a cloacal sample of a diseased broiler breeder pullet in Hungary. Although the conserved nucleotide (e.g., ribosome binding site) and amino acid motifs (e.g., ExxRxNxxxE, S-domain of the viral capsid and motifs in the RNA-dependent RNA polymerase) were identifiable in the chicken picobirnavirus genome, the putative segment 1 showed low (< 30%) amino acid sequence identity to the corresponding proteins of marmot and dromedary PBVs, while segment 2 showed higher (< 70%) amino acid sequence identity to a wolf PBV protein sequence. This is the first full-genome picobirnavirus sequence from a broiler breeder chicken, but the pathogenicity of this virus is still questionable.
Péter Pankovics; Akos Boros; Csaba Nemes; Beatrix Kapusinszky; Eric Delwart; Gábor Reuter. Molecular characterization of a novel picobirnavirus in a chicken. Archives of Virology 2018, 163, 3455 -3458.
AMA StylePéter Pankovics, Akos Boros, Csaba Nemes, Beatrix Kapusinszky, Eric Delwart, Gábor Reuter. Molecular characterization of a novel picobirnavirus in a chicken. Archives of Virology. 2018; 163 (12):3455-3458.
Chicago/Turabian StylePéter Pankovics; Akos Boros; Csaba Nemes; Beatrix Kapusinszky; Eric Delwart; Gábor Reuter. 2018. "Molecular characterization of a novel picobirnavirus in a chicken." Archives of Virology 163, no. 12: 3455-3458.
Lumbricin and its orthologue antimicrobial peptides were typically isolated from annelids. In this report, mRNA for lumbricin and -serendipitously- a novel lumbricin-related mRNA sequence were identified in Eisenia andrei earthworms. The determined mRNA sequences of E. andrei lumbricin and lumbricin-related peptide consist of 477 and 575 nucleotides. The precursors of proline-rich E. andrei lumbricin and the related peptide contain 63 and 59 amino acids, respectively. Phylogenetic analysis indicated close relationship with other annelid lumbricins. Highest expression of both mRNAs appeared in the proximal part of the intestine (pharynx, gizzard), while other tested organs had moderate (body wall, midgut, ovary, metanephridium, seminal vesicles, ventral nerve cord) or low (coelomocytes) levels. During ontogenesis their expression revealed continuous increase in embryos. Following 48 h of in vivo Gram-positive bacteria challenge both mRNAs were significantly elevated in coelomocytes, while Gram-negative bacteria or zymosan stimulation had no detectable effects.
Kornélia Bodó; Akos Boros; Éva Rumpler; László Molnár; Katalin Böröcz; Péter Németh; Peter Engelmann. Identification of novel lumbricin homologues in Eisenia andrei earthworms. Developmental & Comparative Immunology 2018, 90, 41 -46.
AMA StyleKornélia Bodó, Akos Boros, Éva Rumpler, László Molnár, Katalin Böröcz, Péter Németh, Peter Engelmann. Identification of novel lumbricin homologues in Eisenia andrei earthworms. Developmental & Comparative Immunology. 2018; 90 ():41-46.
Chicago/Turabian StyleKornélia Bodó; Akos Boros; Éva Rumpler; László Molnár; Katalin Böröcz; Péter Németh; Peter Engelmann. 2018. "Identification of novel lumbricin homologues in Eisenia andrei earthworms." Developmental & Comparative Immunology 90, no. : 41-46.
In recent years, relatives (bastrovirus, hepelivirus) of hepeviruses (family Hepeviridae) have been reported in a variety of vertebrate hosts. Preliminary studies indicated that inter-viral family recombination events at the junction of the genomes that encodes non-structural (ORF1) and structural protein (ORF2) were implicated in the genesis of hepeviruses. Using viral metagenomics, next generation sequencing and RT-PCR techniques a genetically divergent hepevirus-like RNA virus was identified and characterized from agile frog (Rana dalmatina) tadpoles living in aquatic environment in three natural ponds (Mélymocsár, Lake Ilona and Lake Katlan) in the Pilis Mountains, in Hungary. The complete genome of the viral strain agile frog/RD6/2015/HUN (MH330682) is 7188 nt long including a 48-nt 5′ and a 122-nt 3′ non-coding region. Sequence analysis indicated that the agile frog/RD6/2015/HUN genome has potentially three non-overlapping ORFs. ORF1 (4740 nt/1579aa) has a hepevirus-like non-structural genome organization and encodes several hepevirus-like amino acid sequence motifs. The ORF2 is a potential capsid protein. The functions of the ORF3 were not predictable. The study virus was present in 18 (46%) of the 39 faecal specimen pools from agile frog tadpoles. The taxonomic position of this novel virus is presently unknown.
Gábor Reuter; Akos Boros; Zoltán Tóth; Beatrix Kapusinszky; Eric Delwart; Péter Pankovics. Detection of a novel RNA virus with hepatitis E virus-like non-structural genome organization in amphibian, agile frog (Rana dalmatina) tadpoles. Infection, Genetics and Evolution 2018, 65, 112 -116.
AMA StyleGábor Reuter, Akos Boros, Zoltán Tóth, Beatrix Kapusinszky, Eric Delwart, Péter Pankovics. Detection of a novel RNA virus with hepatitis E virus-like non-structural genome organization in amphibian, agile frog (Rana dalmatina) tadpoles. Infection, Genetics and Evolution. 2018; 65 ():112-116.
Chicago/Turabian StyleGábor Reuter; Akos Boros; Zoltán Tóth; Beatrix Kapusinszky; Eric Delwart; Péter Pankovics. 2018. "Detection of a novel RNA virus with hepatitis E virus-like non-structural genome organization in amphibian, agile frog (Rana dalmatina) tadpoles." Infection, Genetics and Evolution 65, no. : 112-116.
An enteric outbreak with high mortality (34/52, 65.4%) was recorded in 2014 in home-reared estrildid finches (Estrildidae) in Hungary. A novel passerivirus was identified in a diseased violet-eared waxbill using viral metagenomics and confirmed by RT-(q)PCR. The complete genome of finch picornavirus strain waxbill/DB01/HUN/2014 (MF977321) showed the highest amino acid sequence identity of 38.9%, 61.6%, 69.6% in P1cap, 2Chel and 3CproDpol, respectively, to passerivirus A1 (GU182406). A high viral load (6.58 × 1010 genomic copies/ml) was measured in a cloacal specimen and in the tissues (spinal cord, lung, and the intestines) of two additional affected finches. In addition to intestinal symptoms (diarrhoea), the presence of extra-intestinal virus suggests a generalized infection in this fatal disease, for which the passerivirus might be a causative agent.
Péter Pankovics; Akos Boros; Tung Phan; Eric Delwart; Gábor Reuter. A novel passerivirus (family Picornaviridae) in an outbreak of enteritis with high mortality in estrildid finches (Uraeginthus sp.). Archives of Virology 2018, 163, 1063 -1071.
AMA StylePéter Pankovics, Akos Boros, Tung Phan, Eric Delwart, Gábor Reuter. A novel passerivirus (family Picornaviridae) in an outbreak of enteritis with high mortality in estrildid finches (Uraeginthus sp.). Archives of Virology. 2018; 163 (4):1063-1071.
Chicago/Turabian StylePéter Pankovics; Akos Boros; Tung Phan; Eric Delwart; Gábor Reuter. 2018. "A novel passerivirus (family Picornaviridae) in an outbreak of enteritis with high mortality in estrildid finches (Uraeginthus sp.)." Archives of Virology 163, no. 4: 1063-1071.
The complete genome of goose picornavirus 1 (GPV-1) strain goose/NLSZK2/HUN/2013 (MF358731) was determined by RT-PCR and next-generation sequencing from a cloacal sample of a migratory waterfowl, greater white-fronted goose (Anser albifrons) in Hungary. The genome of GPV-1 shows an L-3-3-4 organization pattern with a 5’-terminal origin of replication (ORI) region, a type-IV IRES, and an Hbox/NC-type 2A protein. This virus showed the highest overall sequence identity to the members of the genus Kobuvirus, although the phylogenetic position of GPV-1 is different in the analyzed P1, 2C and 3CD phylogenetic trees, which further increases the diversity of known avian picornaviruses.
Akos Boros; Péter Pankovics; Peter Simmonds; Tamás Kiss; Tung Phan; Eric Delwart; Gábor Reuter. Genomic analysis of a novel picornavirus from a migratory waterfowl, greater white-fronted goose (Anser albifrons). Archives of Virology 2017, 163, 1087 -1090.
AMA StyleAkos Boros, Péter Pankovics, Peter Simmonds, Tamás Kiss, Tung Phan, Eric Delwart, Gábor Reuter. Genomic analysis of a novel picornavirus from a migratory waterfowl, greater white-fronted goose (Anser albifrons). Archives of Virology. 2017; 163 (4):1087-1090.
Chicago/Turabian StyleAkos Boros; Péter Pankovics; Peter Simmonds; Tamás Kiss; Tung Phan; Eric Delwart; Gábor Reuter. 2017. "Genomic analysis of a novel picornavirus from a migratory waterfowl, greater white-fronted goose (Anser albifrons)." Archives of Virology 163, no. 4: 1087-1090.
A large, highly prolific swine farm in Hungary had a 2-year history of neurologic disease among newly weaned (25- to 35-day-old) pigs, with clinical signs of posterior paraplegia and a high mortality rate. Affected pigs that were necropsied had encephalomyelitis and neural necrosis. Porcine astrovirus type 3 was identified by reverse transcription PCR and in situ hybridization in brain and spinal cord samples in 6 animals from this farm. Among tissues tested by quantitative RT-PCR, the highest viral loads were detected in brainstem and spinal cord. Similar porcine astrovirus type 3 was also detected in archived brain and spinal cord samples from another 2 geographically distant farms. Viral RNA was predominantly restricted to neurons, particularly in the brainstem, cerebellum (Purkinje cells), and cervical spinal cord. Astrovirus was generally undetectable in feces but present in respiratory samples, indicating a possible respiratory infection. Astrovirus could cause common, neuroinvasive epidemic disease.
Akos Boros; Á. Boros Et Al.; Péter Pankovics; Hunor Bíró; Patricia A. Pesavento; Tung Phan; Eric Delwart; Gábor Reuter. Outbreaks of Neuroinvasive Astrovirus Associated with Encephalomyelitis, Weakness, and Paralysis among Weaned Pigs, Hungary. Emerging Infectious Diseases 2017, 23, 1982 -1993.
AMA StyleAkos Boros, Á. Boros Et Al., Péter Pankovics, Hunor Bíró, Patricia A. Pesavento, Tung Phan, Eric Delwart, Gábor Reuter. Outbreaks of Neuroinvasive Astrovirus Associated with Encephalomyelitis, Weakness, and Paralysis among Weaned Pigs, Hungary. Emerging Infectious Diseases. 2017; 23 (12):1982-1993.
Chicago/Turabian StyleAkos Boros; Á. Boros Et Al.; Péter Pankovics; Hunor Bíró; Patricia A. Pesavento; Tung Phan; Eric Delwart; Gábor Reuter. 2017. "Outbreaks of Neuroinvasive Astrovirus Associated with Encephalomyelitis, Weakness, and Paralysis among Weaned Pigs, Hungary." Emerging Infectious Diseases 23, no. 12: 1982-1993.
Ljungan and Sebokele viruses are thought to be rodent-borne (picorna)viruses in the genus Parechovirus. Using random amplification and next generation sequencing method a novel Ljungan/Sebokele-like picornavirus was identified in birds of prey. Viral RNA was detected in total of 1 (9%) of the 11 and 2 (28.6%) of the 7 faecal samples from common kestrels and red-footed falcons in Hungary, respectively. High faecal viral RNA load (4.77×10(6) genomic copies/ml) measured by qPCR. The complete genome of picornavirus strain falcon/HA18_080/2014/HUN (KY645497) is 7964-nucleotide (nt) long including a 867-nt 5'end and a 101-nt 3'end (excluding the poly(A)-tail). Falcon/HA18_080/2014/HUN has type-II IRES related to hunnivirus IRES, encodes a polyprotein lacking a leader protein, a VP0 maturation cleavage site and it predicted to encode three 2A proteins (2A1(NPG↓P), 2A2(NPG↓P) and 2A3(H-Box/NC)), two of them end with 'ribosome-skipping' sites (DxExNPG(↓)P). Sequence analyses indicated that the ORF1 (6996nt) polyprotein (2331 amino acid - aa) of falcon/HA18_080/2014/HUN shares the highest aa identity, 59% and 57%, to the corresponding polyproteins of Ljungan and Sebokele viruses. This study reports the identification and complete genome characterization of a novel Ljungan/Sebokele-like picornavirus in faeces of birds of prey which suggests that the genetic diversity and the potential host species spectrum of Ljungan/Sebokele-like viruses in genus Parechovirus are wider than previously thought.
Péter Pankovics; Ákos Boros; Róbert Mátics; Beatrix Kapusinszky; Eric Delwart; Gábor Reuter. Ljungan/Sebokele-like picornavirus in birds of prey, common kestrel ( Falco tinnunculus ) and red-footed falcon ( F. vespertinus ). Infection, Genetics and Evolution 2017, 55, 14 -19.
AMA StylePéter Pankovics, Ákos Boros, Róbert Mátics, Beatrix Kapusinszky, Eric Delwart, Gábor Reuter. Ljungan/Sebokele-like picornavirus in birds of prey, common kestrel ( Falco tinnunculus ) and red-footed falcon ( F. vespertinus ). Infection, Genetics and Evolution. 2017; 55 ():14-19.
Chicago/Turabian StylePéter Pankovics; Ákos Boros; Róbert Mátics; Beatrix Kapusinszky; Eric Delwart; Gábor Reuter. 2017. "Ljungan/Sebokele-like picornavirus in birds of prey, common kestrel ( Falco tinnunculus ) and red-footed falcon ( F. vespertinus )." Infection, Genetics and Evolution 55, no. : 14-19.
Using random amplification and high-throughput sequencing technology a novel picornavirus with dicistronic genome organization and genetically related to canine picodicistrovirus (genus Dicipivirus, family Picornaviridae) was identified and characterized in Northern white-breasted hedgehogs. Hedgehog dicipivirus (hedgehog/H14/2015/HUN, MF188967) was detected in 15 (75%) of 20 faecal specimens by RT-PCR with high viral loads (up to 8.2x10(8) genomic copies/ml faeces). Hedgehog dicipivirus RNA was also identified in blood, ear skin, abdominal muscle and liver tissues. While the general dicistronic genome organization of hedgehog/H14/2015/HUN is similar to canine picodicistrovirus (5'UTR-P1-IGR-P2/P3-3UTR) there are some unique genome characteristics within the untranslated regions, especially in the functional IRES elements. This study reports the putative second member of the genus Dicipivirus, in a novel host species.
Gábor Reuter; Akos Boros; Gabor Foldvari; Sarolta Szekeres; Róbert Mátics; Beatrix Kapusinszky; Eric Delwart; Péter Pankovics. Dicipivirus (family Picornaviridae) in wild Northern white-breasted hedgehog (Erinaceus roumanicus). Archives of Virology 2017, 163, 175 -181.
AMA StyleGábor Reuter, Akos Boros, Gabor Foldvari, Sarolta Szekeres, Róbert Mátics, Beatrix Kapusinszky, Eric Delwart, Péter Pankovics. Dicipivirus (family Picornaviridae) in wild Northern white-breasted hedgehog (Erinaceus roumanicus). Archives of Virology. 2017; 163 (1):175-181.
Chicago/Turabian StyleGábor Reuter; Akos Boros; Gabor Foldvari; Sarolta Szekeres; Róbert Mátics; Beatrix Kapusinszky; Eric Delwart; Péter Pankovics. 2017. "Dicipivirus (family Picornaviridae) in wild Northern white-breasted hedgehog (Erinaceus roumanicus)." Archives of Virology 163, no. 1: 175-181.
In this study, the complete genome of a novel picornavirus called harrier picornavirus 1 (HaPV-1) strain harrier/MR-01/HUN/2014 (KY488458) was sequenced and analysed from a cloacal sample of a threatened, carnivorous wild bird, western marsh harrier (Circus aeruginosus). HaPV-1 was detectable from 2 of the 3 samples from harriers. HaPV-1 is phylogenetically related to megriviruses (genus Megrivirus) from domestic chicken, turkey and duck, showing a similar genome organization pattern; it also has an avian picornavirus-like “Unit A” motif in the 3’ UTR. Unlike the type-IV internal ribosomal entry site (IRES) of megriviruses, HaPV-1 is predicted to contain a type–II–like IRES, suggesting modular exchange of IRES elements between picornavirus genomes.
Akos Boros; Péter Pankovics; Róbert Mátics; Ádám Adonyi; Nóra Bolba; Tung Phan; Eric Delwart; Gábor Reuter. Genome characterization of a novel megrivirus-related avian picornavirus from a carnivorous wild bird, western marsh harrier (Circus aeruginosus). Archives of Virology 2017, 162, 2781 -2789.
AMA StyleAkos Boros, Péter Pankovics, Róbert Mátics, Ádám Adonyi, Nóra Bolba, Tung Phan, Eric Delwart, Gábor Reuter. Genome characterization of a novel megrivirus-related avian picornavirus from a carnivorous wild bird, western marsh harrier (Circus aeruginosus). Archives of Virology. 2017; 162 (9):2781-2789.
Chicago/Turabian StyleAkos Boros; Péter Pankovics; Róbert Mátics; Ádám Adonyi; Nóra Bolba; Tung Phan; Eric Delwart; Gábor Reuter. 2017. "Genome characterization of a novel megrivirus-related avian picornavirus from a carnivorous wild bird, western marsh harrier (Circus aeruginosus)." Archives of Virology 162, no. 9: 2781-2789.
In this case study, a co-infection with coxsackievirus A5 (family Picornaviridae) and norovirus GII.4 (family Caliciviridae) was detected by RT-PCR in a faecal sample from a six-year-old girl with symptoms of severe acute encephalopathy subsequently diagnosed as the intermittent form of maple syrup urine disease (MSUD). The two co-infecting viruses, which had been detected previously, appeared to have triggered the underlying metabolic disorder. Here, we describe the genotyping of the viruses, as well as the chronological course, laboratory test results, and clinical presentation of this case, which included recurrent vomiting without diarrhoea, metabolic acidosis, unconsciousness, seizure and circulatory collapse, but with a positive final outcome.
Ákos Boros; Péter Pankovics; Sándor Kőmíves; Zoltán Liptai; Sarolta Dobner; Enikő Ujhelyi; György Várallyay; Petra Zsidegh; Nóra Bolba; Gábor Reuter. Co-infection with coxsackievirus A5 and norovirus GII.4 could have been the trigger of the first episode of severe acute encephalopathy in a six-year-old child with the intermittent form of maple syrup urine disease (MSUD). Archives of Virology 2017, 162, 1757 -1763.
AMA StyleÁkos Boros, Péter Pankovics, Sándor Kőmíves, Zoltán Liptai, Sarolta Dobner, Enikő Ujhelyi, György Várallyay, Petra Zsidegh, Nóra Bolba, Gábor Reuter. Co-infection with coxsackievirus A5 and norovirus GII.4 could have been the trigger of the first episode of severe acute encephalopathy in a six-year-old child with the intermittent form of maple syrup urine disease (MSUD). Archives of Virology. 2017; 162 (6):1757-1763.
Chicago/Turabian StyleÁkos Boros; Péter Pankovics; Sándor Kőmíves; Zoltán Liptai; Sarolta Dobner; Enikő Ujhelyi; György Várallyay; Petra Zsidegh; Nóra Bolba; Gábor Reuter. 2017. "Co-infection with coxsackievirus A5 and norovirus GII.4 could have been the trigger of the first episode of severe acute encephalopathy in a six-year-old child with the intermittent form of maple syrup urine disease (MSUD)." Archives of Virology 162, no. 6: 1757-1763.