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The occurrence of several aquatic contaminants, including pharmaceuticals, were investigated in seawater samples collected from effluent-dominated sites in Indonesia: 4 sites in Jakarta Bay and one on the north coast of Central Java. The data presented in this preliminary study provide a snapshot of seawater quality in these areas. Results show that nutrient parameters exceeded the Indonesian Standard Quality of Seawater limits, and some metals were also present. Interestingly, high concentrations of paracetamol were detected at Angke (610 ng/L) and Ancol (420 ng/L), both in Jakarta Bay. To date, this is the first study to report the presence of paracetamol (acetaminophen) in the coastal waters around Indonesia. The high concentrations detected, compared to other levels reported in scientific literature, raise concerns about the environmental risks associated with long-term exposure and, especially, the impact on nearby shellfish farms. Given pharmaceuticals' consideration as emerging contaminants, these data suggest further investigations are needed.
Wulan Koagouw; Zainal Arifin; George W.J. Olivier; Corina Ciocan. High concentrations of paracetamol in effluent dominated waters of Jakarta Bay, Indonesia. Marine Pollution Bulletin 2021, 169, 112558 .
AMA StyleWulan Koagouw, Zainal Arifin, George W.J. Olivier, Corina Ciocan. High concentrations of paracetamol in effluent dominated waters of Jakarta Bay, Indonesia. Marine Pollution Bulletin. 2021; 169 ():112558.
Chicago/Turabian StyleWulan Koagouw; Zainal Arifin; George W.J. Olivier; Corina Ciocan. 2021. "High concentrations of paracetamol in effluent dominated waters of Jakarta Bay, Indonesia." Marine Pollution Bulletin 169, no. : 112558.
Pharmaceuticals pose a major threat to the marine environment, and several studies have recently described their negative effects on marine organisms. Pharmaceutical compounds are constantly being released into aquatic ecosystems, and chronic exposure, even at low concentrations, may have a major impact on marine organisms. The purpose of the present study is to evaluate the biological changes induced by one of the most widely used pharmaceuticals—paracetamol—in the blue mussel Mytilus edulis, after a long-term exposure at environmentally relevant concentrations. We present our data alongside and in comparison with results from a previous short-term exposure, to demonstrate the significance of exposure period on the effects of paracetamol in adult blue mussels. After 24 days of laboratory exposure, seven potential target genes were selected to examine toxicological effects in mussels’ gonads and possible disruptive effects on reproductive processes. The results show the modulation of some important reproduction-related genes: estrogen receptor-2 (ER2), vitelline envelope zona pellucida domain-9 (V9), and vitellogenin (VTG). Variations in mRNA expression of four other genes involved in apoptosis (HSP70, CASP8, BCL2, and FAS) are also highlighted. Histopathological alterations caused by paracetamol, together with neutral red retention time response in mussels’ hemocytes, are presented herein. Overall, this study highlights the exacerbated effects of low concentration of paracetamol after chronic exposure, similar to the damage induced by higher concentrations in a short exposure scenario, thus emphasizing the importance of length of exposure period when studying the effects of this substance. Additionally, this study also discusses the potential of paracetamol to inflict several major changes in the reproductive system of mussels and thus possibly affect the survival of populations.
Wulan Koagouw; Nicolas A. Stewart; Corina Ciocan. Long-term exposure of marine mussels to paracetamol: is time a healer or a killer? Environmental Science and Pollution Research 2021, 1 -14.
AMA StyleWulan Koagouw, Nicolas A. Stewart, Corina Ciocan. Long-term exposure of marine mussels to paracetamol: is time a healer or a killer? Environmental Science and Pollution Research. 2021; ():1-14.
Chicago/Turabian StyleWulan Koagouw; Nicolas A. Stewart; Corina Ciocan. 2021. "Long-term exposure of marine mussels to paracetamol: is time a healer or a killer?" Environmental Science and Pollution Research , no. : 1-14.
Plastics and synthetic materials are polluting the world's oceans. In this study we exposed juvenile mussels, Mytilus edulis, to glass reinforced plastic (GRP) dust, under laboratory conditions. The study ran for a period of 7 days, to test for the morphological and potential physiological impacts of GRP. Infrared spectroscopy has revealed that the GRP resin material is poly diallyl phthalate. In mussels, particulate glass and plastics were detected in the digestive tubules and gills, with a suite of inflammatory features observed in all examined organs. In parallel, we observed the effect of powdered GRP on swimming behaviour and survival of water fleas, Daphnia magna. Polymer particles and fibreglass adhered to the filament hairs on appendages, including the caudal spine, in exposed organisms. Most importantly, swimming impairment and sinking of the animals were recorded shortly after exposure. The potential implications for severe localized impact of GRP on aquatic environment are discussed.
Corina Ciocan; Petra Kristova; Claude Annels; Mael Derjean; Laurence Hopkinson. Glass reinforced plastic (GRP) a new emerging contaminant - First evidence of GRP impact on aquatic organisms. Marine Pollution Bulletin 2020, 160, 111559 .
AMA StyleCorina Ciocan, Petra Kristova, Claude Annels, Mael Derjean, Laurence Hopkinson. Glass reinforced plastic (GRP) a new emerging contaminant - First evidence of GRP impact on aquatic organisms. Marine Pollution Bulletin. 2020; 160 ():111559.
Chicago/Turabian StyleCorina Ciocan; Petra Kristova; Claude Annels; Mael Derjean; Laurence Hopkinson. 2020. "Glass reinforced plastic (GRP) a new emerging contaminant - First evidence of GRP impact on aquatic organisms." Marine Pollution Bulletin 160, no. : 111559.
A growing body of literature suggests that pharmaceutical contamination poses an increasing risk to marine ecosystems. Paracetamol or acetaminophen is the most widely used medicine in the world and has recently been detected in seawater. Here, we present the results of 7 days’ exposure of blue mussel adults to 40 ng/L, 250 ng/L and 100 μg/L of paracetamol. Histopathology shows that haemocytic infiltration is the most observed condition in the exposed mussels. The mRNA expression of VTG, V9, ER2, HSP70, CASP8, BCL2 and FAS in mussel gonads present different patterns of downregulation. VTG and CASP8 mRNA expression show downregulation in all exposed mussels, irrespective of sex. The V9, HSP70, BCL2 and FAS transcripts follow a concentration-dependent variation in gene expression and may therefore be considered good biomarker candidates. ER2 mRNA expression shows a downregulated trend, with a clearer dose-response relationship in males. In conclusion, this study suggests that paracetamol has the potential to alter the expression of several genes related to processes occurring in the reproductive system and may therefore impair reproduction in blue mussels.
Wulan Koagouw; Corina Ciocan. Effects of short-term exposure of paracetamol in the gonads of blue mussels Mytilus edulis. Environmental Science and Pollution Research 2019, 27, 30933 -30944.
AMA StyleWulan Koagouw, Corina Ciocan. Effects of short-term exposure of paracetamol in the gonads of blue mussels Mytilus edulis. Environmental Science and Pollution Research. 2019; 27 (25):30933-30944.
Chicago/Turabian StyleWulan Koagouw; Corina Ciocan. 2019. "Effects of short-term exposure of paracetamol in the gonads of blue mussels Mytilus edulis." Environmental Science and Pollution Research 27, no. 25: 30933-30944.
Wulan Koagouw; Corina Ciocan. Impact of Metformin and Increased Temperature on Blue Mussels Mytilus edulis - Evidence for Synergism. Journal of Shellfish Research 2018, 37, 467 -474.
AMA StyleWulan Koagouw, Corina Ciocan. Impact of Metformin and Increased Temperature on Blue Mussels Mytilus edulis - Evidence for Synergism. Journal of Shellfish Research. 2018; 37 (3):467-474.
Chicago/Turabian StyleWulan Koagouw; Corina Ciocan. 2018. "Impact of Metformin and Increased Temperature on Blue Mussels Mytilus edulis - Evidence for Synergism." Journal of Shellfish Research 37, no. 3: 467-474.
Corina M. Ciocan; Elena Cubero-Leon; Christophe Minier; Jeanette M. Rotchell. Correction: Identification of Reproduction-Specific Genes Associated with Maturation and Estrogen Exposure in a Marine Bivalve Mytilus edulis. PLoS ONE 2015, 10, e0132080 .
AMA StyleCorina M. Ciocan, Elena Cubero-Leon, Christophe Minier, Jeanette M. Rotchell. Correction: Identification of Reproduction-Specific Genes Associated with Maturation and Estrogen Exposure in a Marine Bivalve Mytilus edulis. PLoS ONE. 2015; 10 (7):e0132080.
Chicago/Turabian StyleCorina M. Ciocan; Elena Cubero-Leon; Christophe Minier; Jeanette M. Rotchell. 2015. "Correction: Identification of Reproduction-Specific Genes Associated with Maturation and Estrogen Exposure in a Marine Bivalve Mytilus edulis." PLoS ONE 10, no. 7: e0132080.
Intersex, the appearance of female characteristics in male gonads, has been identified in several aquatic species. It is a widespread phenomenon in populations of the bivalve, Scrobicularia plana, from the southwest coast of the U.K. Genes previously identified as differentially expressed (ferritin, testicular haploid expressed gene, THEG, proliferating cell nuclear antigen, PCNA; receptor activated protein kinase C, RACK; cytochrome B, CYB; and cytochrome c oxidase 1, COX1) in intersex clams relative to normal male clams, were selected for characterisation and an environmental survey of the Channel region. Transcripts were significantly differentially expressed at sites with varying intersex incidence and contaminant burdens. Significant correlations between specific gene expressions, key contaminants and sampling locations have been identified, though no single gene was associated with intersex incidence. The results highlight the difficulty in understanding the intersex phenomenon in molluscs where there is still a lack of knowledge on the control of normal reproduction.
Corina M. Ciocan; Elena Cubero-Leon; William J. Langston; Nick Pope; Keith Cornelius; E.M. Hill; Diana Álvarez-Muñoz; Paolo Indiveri; Adélaïde Lerebours; Christophe Minier; Jeanette M. Rotchell. Intersex related gene expression profiles in clams Scrobicularia plana: Molecular markers and environmental application. Marine Pollution Bulletin 2015, 95, 610 -617.
AMA StyleCorina M. Ciocan, Elena Cubero-Leon, William J. Langston, Nick Pope, Keith Cornelius, E.M. Hill, Diana Álvarez-Muñoz, Paolo Indiveri, Adélaïde Lerebours, Christophe Minier, Jeanette M. Rotchell. Intersex related gene expression profiles in clams Scrobicularia plana: Molecular markers and environmental application. Marine Pollution Bulletin. 2015; 95 (2):610-617.
Chicago/Turabian StyleCorina M. Ciocan; Elena Cubero-Leon; William J. Langston; Nick Pope; Keith Cornelius; E.M. Hill; Diana Álvarez-Muñoz; Paolo Indiveri; Adélaïde Lerebours; Christophe Minier; Jeanette M. Rotchell. 2015. "Intersex related gene expression profiles in clams Scrobicularia plana: Molecular markers and environmental application." Marine Pollution Bulletin 95, no. 2: 610-617.
Intersex, the appearance of female characteristics in male gonads, has been identified in a wide range of aquatic species worldwide, yet the underpinning molecular etiology remains uncharacterized. The presence of intersex has been shown to be a widespread phenomenon in bivalve, S. plana, populations from the southwest coast of the U.K., as well as inducible in an experimental exposure regime using endocrine disrupting compounds (EDCs). Herein, we use the suppressive subtractive hybridization approach to isolate differentially expressed transcripts in S. plana males exhibiting intersex. Transcripts involved in cell signaling, cell cycle control, energy production/metabolism, microtubule assembly, and sperm physiology are all highlighted as differentially expressed in intersex male clams. These provide both an insight into the molecular mechanisms of action involved in the development of intersex, as well as facilitating potential molecular-level "early warning" biomarkers of the condition.
Corina Ciocan; Elena Cubero Leon; Mika R. Peck; William J. Langston; Nick Pope; Christophe Minier; Jeanette M. Rotchell. Intersex in Scrobicularia plana: Transcriptomic Analysis Reveals Novel Genes Involved in Endocrine Disruption. Environmental Science & Technology 2012, 46, 12936 -12942.
AMA StyleCorina Ciocan, Elena Cubero Leon, Mika R. Peck, William J. Langston, Nick Pope, Christophe Minier, Jeanette M. Rotchell. Intersex in Scrobicularia plana: Transcriptomic Analysis Reveals Novel Genes Involved in Endocrine Disruption. Environmental Science & Technology. 2012; 46 (23):12936-12942.
Chicago/Turabian StyleCorina Ciocan; Elena Cubero Leon; Mika R. Peck; William J. Langston; Nick Pope; Christophe Minier; Jeanette M. Rotchell. 2012. "Intersex in Scrobicularia plana: Transcriptomic Analysis Reveals Novel Genes Involved in Endocrine Disruption." Environmental Science & Technology 46, no. 23: 12936-12942.
Members of the vertebrate CYP3A subfamily are involved in the metabolism of steroids and a wide range of xenobiotics. In this study two CYP3A-like mRNAs have been isolated from the mussel (Mytilus edulis), and their seasonal expression profile and modulation by estrogens examined. Sexual dimorphism of CYP3A-like mRNA expression was not observed in mussel gonads of individuals collected throughout a year. Nevertheless, natural variation in gonadal CYP3A-like mRNA expression was observed, with highest levels of CYP3A isoform1 and lowest levels of CYP3A isoform2 mRNA during the maturation and spawning season. Exposure to a 10% sewage treatment works extract did not result in any significant changes in mRNA expression of CYP3A-like. In contrast, exposure to E2 (200 ng/L) and TBT (100 ng/L) significantly down-regulated the expression of CYP3A-like isoform1 but not CYP3A-like isoform2 suggesting differential regulation.
Elena Cubero-Leon; A. Mirel Puinean; Pierre Labadie; Corina Ciocan; Naoki Itoh; Mitsuyo Kishida; Makoto Osada; Christophe Minier; Elizabeth M. Hill; Jeanette M. Rotchell. Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors. Marine Environmental Research 2012, 74, 32 -39.
AMA StyleElena Cubero-Leon, A. Mirel Puinean, Pierre Labadie, Corina Ciocan, Naoki Itoh, Mitsuyo Kishida, Makoto Osada, Christophe Minier, Elizabeth M. Hill, Jeanette M. Rotchell. Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors. Marine Environmental Research. 2012; 74 ():32-39.
Chicago/Turabian StyleElena Cubero-Leon; A. Mirel Puinean; Pierre Labadie; Corina Ciocan; Naoki Itoh; Mitsuyo Kishida; Makoto Osada; Christophe Minier; Elizabeth M. Hill; Jeanette M. Rotchell. 2012. "Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors." Marine Environmental Research 74, no. : 32-39.
The aim of this study is to develop a normalization method for real-time PCR data by analyzing the most stably expressed control genes in mussel (Mytilus edulis) reproductive tissue.
Elena Cubero-Leon; Corina M. Ciocan; Christophe Minier; Jeanette M. Rotchell. Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure. Environmental Science and Pollution Research 2012, 19, 2728 -2733.
AMA StyleElena Cubero-Leon, Corina M. Ciocan, Christophe Minier, Jeanette M. Rotchell. Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure. Environmental Science and Pollution Research. 2012; 19 (7):2728-2733.
Chicago/Turabian StyleElena Cubero-Leon; Corina M. Ciocan; Christophe Minier; Jeanette M. Rotchell. 2012. "Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure." Environmental Science and Pollution Research 19, no. 7: 2728-2733.
While it is established that vertebrate-like steroids, particularly estrogens (estradiol, estrone) and androgens (testosterone), are present in various tissues of molluscs, it is still unclear what role these play in reproductive endocrinology in such organisms. This is despite the significant commercial shellfishery interest in several bivalve species and their decline. Using suppression subtraction hybridisation of mussel gonad samples at two stages (early and mature) of gametogenesis and (in parallel) following controlled laboratory estrogen exposure, we isolate several differentially regulated genes including testis-specific kinases, vitelline lysin and envelope sequences. The differentially expressed mRNAs isolated provide evidence that mussels may be impacted by exogenous estrogen exposure.
Corina M. Ciocan; Elena Cubero-Leon; Christophe Minier; Jeanette M. Rotchell. Identification of Reproduction-Specific Genes Associated with Maturation and Estrogen Exposure in a Marine Bivalve Mytilus edulis. PLOS ONE 2011, 6, e22326 .
AMA StyleCorina M. Ciocan, Elena Cubero-Leon, Christophe Minier, Jeanette M. Rotchell. Identification of Reproduction-Specific Genes Associated with Maturation and Estrogen Exposure in a Marine Bivalve Mytilus edulis. PLOS ONE. 2011; 6 (7):e22326.
Chicago/Turabian StyleCorina M. Ciocan; Elena Cubero-Leon; Christophe Minier; Jeanette M. Rotchell. 2011. "Identification of Reproduction-Specific Genes Associated with Maturation and Estrogen Exposure in a Marine Bivalve Mytilus edulis." PLOS ONE 6, no. 7: e22326.
Mytilus edulis were exposed to 17β-estradiol (E2) and the synthetic estrogens ethinyl estradiol (EE2) and estradiol benzoate (EB) for 10 days. Two exposures were performed to determine their effect on vitellogenin (VTG) and estrogen receptor 2 (ER2) mRNA expression at different stages of the reproductive cycle. Significant natural variation was not observed in VTG mRNA expression, though ER2 mRNA expression displayed significantly lower values during January, February and July compared with other times of the year. A significant increase in VTG and ER2 mRNA expression was observed in mussels exposed to estrogens at the early stage of gametogenesis. In contrast, mature mussels displayed no statistically significant change in the VTG or ER2 mRNA expression. The data presented suggests that the reproductive physiology of molluscs, in terms of VTG and ER2 mRNA expression, may be susceptible to damage by environmental estrogens at certain points in their gametogenesis process.
Corina M. Ciocan; Elena Cubero-Leon; Alin M. Puinean; Elizabeth M. Hill; Christophe Minier; Makoto Osada; Kate Fenlon; Jeanette M. Rotchell. Effects of estrogen exposure in mussels, Mytilus edulis, at different stages of gametogenesis. Environmental Pollution 2010, 158, 2977 -2984.
AMA StyleCorina M. Ciocan, Elena Cubero-Leon, Alin M. Puinean, Elizabeth M. Hill, Christophe Minier, Makoto Osada, Kate Fenlon, Jeanette M. Rotchell. Effects of estrogen exposure in mussels, Mytilus edulis, at different stages of gametogenesis. Environmental Pollution. 2010; 158 (9):2977-2984.
Chicago/Turabian StyleCorina M. Ciocan; Elena Cubero-Leon; Alin M. Puinean; Elizabeth M. Hill; Christophe Minier; Makoto Osada; Kate Fenlon; Jeanette M. Rotchell. 2010. "Effects of estrogen exposure in mussels, Mytilus edulis, at different stages of gametogenesis." Environmental Pollution 158, no. 9: 2977-2984.
Estrogenic contaminants in the aquatic environment are associated with feminisation of male fish, however their effects on some invertebrate species, such as bivalve molluscs, have yet to be characterised. Gametogenesis represents a critical step in the reproductive process and is subjected to hormonal control by serotonin (5-HT), prostaglandins (synthesised by cyclooxygenases-COX) and steroids such as 17β-estradiol (E2). Here, we examine the responses of 5-HT receptor and COX mRNA expression in mussels, Mytilus edulis, exposed to estrogenic compounds during different stages of their reproductive cycle. In mature mussels, 5-HT receptor mRNA expression decreased following E2 exposure. The opposite trend was observed in mussels at early gametogenesis stages. COX mRNA expression levels at both stages were generally decreased by E2 exposure. Mussels at early gametogenesis stages were also exposed to ethynylestradiol (EE2) and estradiol benzoate (EB) and a significant increase in 5-HT receptor mRNA expression was observed with both xeno-estrogens. COX expression levels were increased with EB exposure but no significant effects were found with EE2 exposure. These results show that the natural estrogen, E2, as well as the synthetic estrogen, EE2, induce alterations, dependent on reproductive stage, in the mRNA expression levels of 5-HT receptor and/or COX in the marine bivalve M. edulis.
Elena Cubero-Leon; Corina M. Ciocan; Elizabeth M. Hill; Makoto Osada; Mitsuyo Kishida; Naoki Itoh; Reki Kondo; Christophe Minier; Jeanette M. Rotchell. Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis). Aquatic Toxicology 2010, 98, 178 -187.
AMA StyleElena Cubero-Leon, Corina M. Ciocan, Elizabeth M. Hill, Makoto Osada, Mitsuyo Kishida, Naoki Itoh, Reki Kondo, Christophe Minier, Jeanette M. Rotchell. Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis). Aquatic Toxicology. 2010; 98 (2):178-187.
Chicago/Turabian StyleElena Cubero-Leon; Corina M. Ciocan; Elizabeth M. Hill; Makoto Osada; Mitsuyo Kishida; Naoki Itoh; Reki Kondo; Christophe Minier; Jeanette M. Rotchell. 2010. "Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis)." Aquatic Toxicology 98, no. 2: 178-187.
Jeanette M. Rotchell; Corina M. Ciocan. Response to Comment on “Conservation of Cancer Genes in the Marine Invertebrate Mytilus edulis”. Environmental Science & Technology 2007, 41, 4832 -4832.
AMA StyleJeanette M. Rotchell, Corina M. Ciocan. Response to Comment on “Conservation of Cancer Genes in the Marine Invertebrate Mytilus edulis”. Environmental Science & Technology. 2007; 41 (13):4832-4832.
Chicago/Turabian StyleJeanette M. Rotchell; Corina M. Ciocan. 2007. "Response to Comment on “Conservation of Cancer Genes in the Marine Invertebrate Mytilus edulis”." Environmental Science & Technology 41, no. 13: 4832-4832.
Disseminated neoplasia has been reported in mussels (Mytilus spp) from numerous locations worldwide. This condition is progressive and fatal and the aetiology is unknown. In vertebrates, oncogenes such as ras, and tumour suppressor genes such as p53, play important roles in carcinogenesis. We have cloned a Mytilus trossulus homologue of the vertebrate ras gene, which shows conserved sequence in regions of functional importance. Neoplastic hemolymph samples derived from M. trossulus have been investigated for the presence of ras gene mutations and changes in expression.
Corina Ciocan; James D. Moore; Jeanette M. Rotchell. The role of ras gene in the development of haemic neoplasia in Mytilus trossulus. Marine Environmental Research 2006, 62, S147 -S150.
AMA StyleCorina Ciocan, James D. Moore, Jeanette M. Rotchell. The role of ras gene in the development of haemic neoplasia in Mytilus trossulus. Marine Environmental Research. 2006; 62 ():S147-S150.
Chicago/Turabian StyleCorina Ciocan; James D. Moore; Jeanette M. Rotchell. 2006. "The role of ras gene in the development of haemic neoplasia in Mytilus trossulus." Marine Environmental Research 62, no. : S147-S150.
Disseminated neoplasia, also called leukemia or hemic neoplasia, has been detected in 15 species of marine bivalve mollusks worldwide. The disease is characterized by the presence of single anaplastic cells with enlarged nuclei and sometimes frequent mitosis, in hemolymph vessels and sinuses. The neoplastic cells gradually replace normal hemocytes leading to the increased mortality of animals. The neoplasia reaches epizootic prevalences in blue mussels, Mytilus trossulus, in some areas, whereas prevalences in Mytilus edulis are generally very low. Mytilus galloprovincialis was suggested to be resistant to the disease although very low prevalences were documented from Spain in the Atlantic Ocean and Italy in the Mediterranean Sea. A case of disseminated neoplasia was discovered in M. galloprovincialis from among 200 specimens studied from the coast of the Romanian Black Sea. Histological preparation revealed the presence of large anaplastic cells with lobed nuclei. This observation extends the geographic range of marine bivalve mollusks with disseminated neoplasia to include the Black Sea.
Corina Ciocan; Inke Sunila. Disseminated neoplasia in blue mussels, Mytilus galloprovincialis, from the Black Sea, Romania. Marine Pollution Bulletin 2005, 50, 1335 -1339.
AMA StyleCorina Ciocan, Inke Sunila. Disseminated neoplasia in blue mussels, Mytilus galloprovincialis, from the Black Sea, Romania. Marine Pollution Bulletin. 2005; 50 (11):1335-1339.
Chicago/Turabian StyleCorina Ciocan; Inke Sunila. 2005. "Disseminated neoplasia in blue mussels, Mytilus galloprovincialis, from the Black Sea, Romania." Marine Pollution Bulletin 50, no. 11: 1335-1339.
Mussels are susceptible to a wide range of environmental toxicants, including carcinogens, and thus are often employed as bioindicator species. To elucidate the molecular aetiology of such neoplastic damage, we have cloned Mytilus edulis homologues of the vertebrate ras proto-oncogene, and p53 tumor suppressor gene. The M. edulisras cDNA encodes a predicted protein of 184 amino acids. The DNA sequence analysis with vertebrate ras sequences demonstrates that the M. edulisras cDNA is highly conserved in regions of functional importance, including mutational hot spots. The partial p53 sequence also demonstrates that M. edulisp53 is highly conserved in two regions of functional importance and that these regions also include four of the five mutational hot spots for this gene. In contrast, the M. edulisp53 sequence shows little similarity to the other published invertebrate p53-like sequences. The cancer gene sequences characterized herein will allow development of specific biomarkers of genotoxic damage.
Corina M. Ciocan; Jeanette M. Rotchell. Conservation of Cancer Genes in the Marine InvertebrateMytilus edulis. Environmental Science & Technology 2005, 39, 3029 -3033.
AMA StyleCorina M. Ciocan, Jeanette M. Rotchell. Conservation of Cancer Genes in the Marine InvertebrateMytilus edulis. Environmental Science & Technology. 2005; 39 (9):3029-3033.
Chicago/Turabian StyleCorina M. Ciocan; Jeanette M. Rotchell. 2005. "Conservation of Cancer Genes in the Marine InvertebrateMytilus edulis." Environmental Science & Technology 39, no. 9: 3029-3033.
Isoforms of metallothionein in the digestive gland of control and experimentally Cd-exposed mussels (Mytilus edulis) (200 microg L(-1) Cd2+ and 400 microg L(-1) Cd2+; 20 days) were studied using the reverse transcriptase-polymerase chain reaction. In addition, glutathione S-transferase (GSTpi) primers were designed to evaluate the reduction in the antioxidant defense systems (glutathione) accompanying the aging process in the same organisms. Following experimental exposure, an accumulation of Cd was observed in the digestive gland of exposed mussels, both adults and juveniles, up to 500 times higher than in the control. An induction of the dimeric form MT20 II was detected in 400 microg L(-1) exposed mussels, as well as a visible inhibition of the monomeric form MT10 IV. After 20 days of exposure juveniles expressed increased GSTpi compared with adults. Results reveal individual variation of both metallothioneins and GSTpi expression among control and Cd2+-exposed mussels of different ages. The ecotoxicological significance of MT utilization in biomonitoring of seawater for trace metals has been considered in light of these results.
Corina M. Ciocan; Jeanette M. Rotchell. Cadmium Induction of Metallothionein Isoforms in Juvenile and Adult Mussel (Mytilus edulis). Environmental Science & Technology 2004, 38, 1073 -1078.
AMA StyleCorina M. Ciocan, Jeanette M. Rotchell. Cadmium Induction of Metallothionein Isoforms in Juvenile and Adult Mussel (Mytilus edulis). Environmental Science & Technology. 2004; 38 (4):1073-1078.
Chicago/Turabian StyleCorina M. Ciocan; Jeanette M. Rotchell. 2004. "Cadmium Induction of Metallothionein Isoforms in Juvenile and Adult Mussel (Mytilus edulis)." Environmental Science & Technology 38, no. 4: 1073-1078.