Rapidly increasing industry has resulted in greater discharge of hazardous chemicals in the soil. In the current study, soil samples were collected from Nanjing mine (32°09′19.29″ N 118°56′57.04″ E) and subjected to heavy metal analysis and microbe isolation. A total of 460 fungi were isolated, and five of these were yeast strains. Most of the strains exhibited tolerance to one metal. Five multimetal tolerant strains were selected and identified as Aspergillus sclerotiorum, Aspergillus aculeatus, Komagataella phaffii, Trichoderma harzianum, and Aspergillus niger. Isolated strains were grown in high concentrations of cadmium (Cd), chromium (Cr) and lead (Pb), for induced-tolerance training. The tolerance index (TI) revealed the highest Cd tolerance of novel K. phaffii strain at 5500 ppm (TI: 0.2). K. phaffii also displayed resistance at 4000 ppm against Cr (TI: 0.32) and Pb (TI: 0.32). In contrast, tolerance training for A. niger was not that successful. K. phaffii also displayed the highest bioaccumulation capacity for Cd (25.23 mg/g), Cu (21.63 mg/g), and Pb (20.63 mg/g) at 200 ppm. Scanning electron microscopy (SEM) explored the morphological changes in the mycelia of stressed fungi. Results of this study describe this delicate approach to be species and metal dependent and suggest a potential utilization of this fungal strain for the bioremediation of contaminated soils.

This exploratory study employed an integrated methodological approach to examine the relationship among several factors for residents in Shanghai when it came to the use of urban park spaces. The study was conducted using two sample groups of 1200 residents each, and around three community parks that contained a variety of recreation-related impacts. Open-ended and closed-ended questionnaires and correspondence analysis were used to reflect residents’ basic attitudes, recreational perception, and satisfaction evaluation by Importance Performance Analysis (IPA) method, and mirrored preferences for future development of community parks. The results suggest that for residents, high levels of satisfaction with landscape and environment were advantages, but recreation space and facility were critical for the perception of the community parks. In comparison, management was found to be an opportunity factor to improve leisure satisfaction. The findings emphasized landscape, environment, space, facility, and management as elements that enhance recreational perception and avoid passive interference.

Alpine meadows grow in alpine regions and play an important role in the production and life of alpine regions. As a unique feature of alpine regions, freeze–thaw cycles (FTCs) affect the growth of alpine meadows. However, with climate change, the change in the freeze–thaw cycle frequency (FTCF) has become obvious. These changes affect the content and distribution of soil moisture and nutrients, as well as the growth of roots in the alpine meadow. Therefore, based on the analysis of FTCF in the Nagqu River Basin, the characteristics of soil moisture, nutrients, and alpine meadow roots are analyzed, thus revealing the influence mechanism of FTCF on the root growth of alpine meadows. The results highlight three major findings. (1) Compared with the low-frequency mode (LFM), the moisture at 0–20, 20–40, and 40–60 cm in the high-frequency mode (HFM) has decreased by 30.74%, 52.89%, and 47.52%, respectively. Additionally, in HFM the contents of soil hydrolysable nitrogen (HN), available K (AK), and microbial biomass carbon (MBC) at the same depth are lower than those in LFM. (2) The original distribution of soil moisture at 0–60 cm has gradually increased from the surface to the bottom. However, with the increase in FTCF, the distribution of the soil moisture now means that the soil moisture at the surface (0–20 cm) and the deeper layers (40–60 cm) is higher than that in the middle (20–40 cm). (3) With the increase in FTCF, the growth mode of alpine meadow roots has changed from vertical extension to horizontal divergence; the distribution range of roots has changed from 0–40 cm to 0–20 cm; the length, surface area, and volume of 0–0.5 mm roots have increased by 20.95 cm, 1.90 cm², and 0.014 cm³; and the corresponding specific gravity has increased by 9.09%, 13.50%, and 12.14%, respectively. This study provides a theoretical basis for predicting the growth mode of alpine meadow roots in the Nagqu River Basin under the influence of climate change and provides guidance for protecting the ecology of alpine regions and mitigating and solving global climate change.

Urban green space has significant social, ecological, cultural and economic value. This study uses social media data to examine the spatiotemporal utilization of major parks in Shanghai and explore the determinants of their recreational attraction. Methods: Based on microblog check-in data between 2012 and 2018 across 17 parks in Shanghai, we investigated the patterns at different temporal scales (weekly, seasonal and annual) and across workdays and weekends by using log-linear regression models. Results: Our findings indicate that both internal and external factors affect park utilization. In particular, the presence of sports facilities significantly contributes to higher visit frequency. Factors such as the number of subway stations nearby, scenic quality and popularity have a positive impact on check-in numbers, while negative factors affecting park use are number of roads, ticket price and average surrounding housing price. Across different temporal scales, the use patterns of visitors have obvious seasonal and monthly tendencies, and the differences of workday and weekend models lie in external factors’ impacts. Conclusions: In order to achieve the goal of better serving the visitors, renewal of urban green spaces in megacities should consider these influential factors, increase sports facilities, subway stations nearby and improve scenic quality, popularity and water quality. This study on spatiotemporal utilization of urban parks can help enhance comprehensive functions of urban parks and be helpful for urban renewal strategies.

Purslane, a fleshy herbaceous plant, plays a pivotal role in various preventive and therapeutic purposes. To date, no report has documented the consequence of salt stress on metabolite accumulation in purslane. Herein, we proposed an insight into the metabolic and physiological traits of purslane under saline stress environments. The gas chromatography‐mass spectrometry analysis was used to scrutinize the metabolic profiling of leaves and roots of two purslane genotypes, Tall Green (TG) and Shandong Wild (SD), under the control and saline exposures. Results revealed that the morphological and physiological traits of leaves and roots of both the tested Portulaca oleracea cultivars in response to salt stress (100 mM and 200 mM) were dramatically changed. Similarly, significant differences were found in the metabolite profiles among samples under salinity stress treatments as compared with the control. Thorough metabolic pathway analysis, 132 different metabolites in response to 28 days of particular salt stress treatments were recognized and quantified in roots and leaves of purslane, including 35 organic acids, 26 amino acids, 20 sugars, 14 sugar alcohols, 20 amines, 13 lipids and sterols, and 4 other acids. In conclusion, this study can be useful for future molecular experiments as a reference to select gene expression levels for the functional characterization of purslane.