Astrocytes exert adverse effects on the brains of individuals with Down syndrome (DS). Although a neurogenic-to-gliogenic shift in the fate-specification step has been reported, the mechanisms and key regulators underlying the accelerated proliferation of astrocyte precursor cells (APCs) in DS remain elusive. Here, we established a human isogenic cell line panel based on DS-specific induced pluripotent stem cells, the XIST-mediated transcriptional silencing system in trisomic chromosome 21, and genome/chromosome-editing technologies to eliminate phenotypic fluctuations caused by genetic variation. The transcriptional responses of genes observed upon XIST induction and/or downregulation are not uniform, and only a small subset of genes show a characteristic expression pattern, which is consistent with the proliferative phenotypes of DS APCs. Comparative analysis and experimental verification using gene modification reveal dose-dependent proliferation-promoting activity of DYRK1A and PIGP on DS APCs. Our collection of human isogenic cell lines provides a comprehensive set of cellular models for further DS investigations.

With many children and young adolescents reporting strong emotional bonds with their pets, the impact of pet ownership on child/adolescent health—especially on their emotional development—has garnered increasing scientific interest. We examined the association between pet ownership in toddlerhood (age 3.5 years) and poor emotional expression in later childhood (age 5.5 years) using propensity score matching within a longitudinal cohort dataset from Japan (n = 31,453). A propensity score for pet ownership was calculated by logistic models based on a comprehensive list of each child’s observed characteristics, including sex, household income, parental education, mother’s employment status, residential environment, number of siblings, and living arrangement. Log-binomial regression analyses using matched samples revealed that children who owned pets during the toddler years were 6% less likely to have a poor emotional expression in later childhood (prevalence ratio = 0.94, 95% confidence interval = 0.90–0.99) compared to those without pets. This suggests that owning pets may provide children with opportunities to control their emotions, and lead to a lower prevalence of poor emotional expression. Pet ownership in toddlerhood may contribute to the development of expression.

Purpose: Approximately 25–60% of COVID-19 patients develop long-term sequelae of the condition known as long COVID. This study aimed to examine sociodemographic and clinical characteristics of long COVID in Japan. Methods: The data of long COVID patients, defined as those who were symptomatic after 28 days from onset, were collected in an outpatient clinic in Tokyo, Japan between 6 January 2020 and 2 October 2021 (N = 1891). Information on age, sex, employment, infection waves, vaccination, impairment in activities of daily living, and symptoms were obtained from electronic medical records. We used linear regression to analyze the association of patients characteristics with performance status. Results: The mean number of days from onset was 77.6 (SD: 71.3). Female, those who had their work hours reduced, on leave, dismissed or retired or not working, were associated with lower performance status. Fatigue, depressive symptom, brain fog, dyspnea, palpitation, body pain, loss of appetite, fever—but not headache, insomnia, loss of smell, loss of taste, hair loss, or cough—were associated with the lower performance status. Conclusion: Sex and employment status were associated with lower performance status in long COVID patients. Studies are needed to elucidate the full picture of the characteristics of long COVID patients.