Ganoderma lingzhi(G. lingzhi),G. sinense,G. applanatum, etc. belongs to theGanodermagenus of polypore mushrooms which contain rich polysaccharides valuable for nutrition and positive medicinal effects. In order to evaluate polysaccharide content inGanodermamycelia obtained in the fermentation process quickly and accurately, in this work we employed infrared spectroscopy to examine differentGanodermastains of samples from diversified sources. Through mid-infrared (mid-IR) spectroscopy, we could identify the most relevant spectral bands required for polysaccharide evaluation, and through near-infrared (NIR) spectroscopy, we could establish the quantification model for making satisfactory prediction of polysaccharide ingredient content. As such, we have achieved an effective and convenient approach to quantitative assessment of the total polysaccharides inGanodermamycelia but also demonstrated that infrared spectroscopy can be a powerful tool for quality control ofGanodermapolysaccharides obtained from industrial production.