Background: Macrophomina phaseolina is one of the most destructive necrotrophic fungal pathogens that infect more than 500 plant species throughout the world. It can grow rapidly in infected plants and subsequently produces a large amount of sclerotia that plugs the vessels, resulting in wilting of the plant. Results: We sequenced and assembled ~49 Mb into 15 super-scaffolds covering 92.83% of the M. phaseolina genome. We predict 14,249 open reading frames (ORFs) of which 9,934 are validated by the transcriptome. This phytopathogen has an abundance of secreted oxidases, peroxidases, and hydrolytic enzymes for degrading cell wall polysaccharides and lignocelluloses to penetrate into the host tissue. To overcome the host plant defense response, M. phaseolina encodes a significant number of P450s, MFS type membrane transporters, glycosidases, transposases, and secondary metabolites in comparison to all sequenced ascomycete species. A strikingly distinct set of carbohydrate esterases (CE) are present in M. phaseolina, with the CE9 and CE10 families remarkably higher than any other fungi. The phenotypic microarray data indicates that M. phaseolina can adapt to a wide range of osmotic and pH environments. As a broad host range pathogen, M. phaseolina possesses a large number of pathogen-host interaction genes including those for adhesion, signal transduction, cell wall breakdown, purine biosynthesis, and potent mycotoxin patulin. Conclusions: The M. phaseolina genome provides a framework of the infection process at the cytological and molecular level which uses a diverse arsenal of enzymatic and toxin tools to destroy the host plants. Further understanding of the M. phaseolina genome-based plant-pathogen interactions will be instrumental in designing rational strategies for disease control, essential to ensuring global agricultural crop production and security.

Jute (Corchorussp.) is one of the most important sources of natural fibre, covering ∼80% of global bast fibre production¹. OnlyCorchorus olitoriusandCorchorus capsularisare commercially cultivated, though there are more than 100Corchorusspecies²in the Malvaceae family. Here we describe high-quality draft genomes of these two species and their comparisons at the functional genomics level to support tailor-designed breeding. The assemblies cover 91.6% and 82.2% of the estimated genome sizes forC. olitoriusandC. capsularis, respectively. In total, 37,031C. olitoriusand 30,096C. capsularisgenes are identified, and most of the genes are validated by cDNA and RNA-seq data. Analyses of clustered gene families and gene collinearity show that jute underwent shared whole-genome duplication ∼18.66 million years (Myr) ago prior to speciation. RNA expression analysis from isolated fibre cells reveals the key regulatory and structural genes involved in fibre formation. This work expands our understanding of the molecular basis of fibre formation laying the foundation for the genetic improvement of jute.

Generally, flooding causes waterlogging or submergence stress which is considered as one of the most important abiotic factors that severely hinders plant growth and development. Plants might not complete their life cycle even in short duration of flooding. As biologically intelligent organisms, plants always try to resist or survive under such adverse circumstances by adapting a wide array of mechanisms including hormonal homeostasis. Under this mechanism, plants try to adapt through diverse morphological, physiological and molecular changes, including the closing of stomata, elongating of petioles, hollow stems or internodes, or maintaining minimum physiological activity to store energy to combat post-flooding stress and to continue normal growth and development. Mainly, ethylene, gibberellins (GA) and abscisic acid (ABA) are directly and/or indirectly involved in hormonal homeostasis mechanisms. Responses of specific genes or transcription factors or reactive oxygen species (ROS) maintain the equilibrium between stomatal opening and closing, which is one of the fastest responses in plants when encountering flooding stress conditions. In this review paper, the sequential steps of some of the hormone-dependent survival mechanisms of plants under flooding stress conditions have been critically discussed.

In fungi, like other eukaryotes, protein turnover is an important cellular process for the controlling of various cellular functions. The ubiquitin-proteasome pathway degrades some selected intracellular proteins and F-box proteins are one of the important components controlling protein degradation. F-box proteins are well studied in different model plants however, their functions in the fungi are not clear yet. This study aimed to identify the genes involved in protein degradation for disease development in the Macrophomina phaseolina fungus. In this research, in silico studies were done to understand the distribution of F-box proteins in pathogenic fungi including Macrophomina phaseolina fungus. Genome-wide analysis indicates that M. phaseolina fungus contained thirty-one F-box proteins throughout its chromosomes. In addition, there are 17, 37, 16, and 21 F-box proteins have been identified from Puccinia graminis, Colletotrichum graminicola, Ustilago maydis, and Phytophthora infestans, respectively. Analyses revealed that selective fungal genomes contain several additional functional domains along with F-box domain. Sequence alignment showed the substitution of amino acid in several F-box proteins; however, gene duplication was not found among these proteins. Phylogenetic analysis revealed that F-box proteins having similar functional domain was highly diverse form each other showing the possibility of various function. Analysis also found that MPH_00568 and MPH_05531 were closely related to rice blast fungus F-box protein MGG_00768 and MGG_13065, respectively, may play an important role for blast disease development. This genome-wide analysis of F-box proteins will be useful for characterization of candidate F-box proteins to understand the molecular mechanisms leading to disease development of M. phaseolina in the host plants.

Deciphering genetic information through next-generation sequencing (NGS) is considered as the basic platform to unveil in details of an organism. However, as it produces short reads that lead to difficulties in assembly, we generated long scaffold-based optical mapping (OM) data of previously sequenced devastating fungus, Macrophomina phaseolina MS6. In the process, KpnI identified as the most effective restriction endonuclease among tested 13, used to digest high molecular weight (HMW) DNA that generated 270,343 genomic DNA molecules size in more than 250 kb. The molecules were assembled and constructed 12 super-scaffolds (terminated with telomeric blunt-ends and denoted as chromosomes) that were aligned with NGS generated 17 (out of 88 reduced from 94) reference scaffolds. The state-of-the-art technology revealed concordances and different discordances viz., inversions, low-quality assembly, gaps, overlaps followed to correct the NGS misassembles. Based on the results, OM generated improved and validated assembly advance our understanding of the chromosome evolution of fungi. This furnished data might be considered as valuable resources to accelerate the precise planning for the protection of M. phaseolina MS6 infected sequenced crops through developing the cross-talk phenomenon between the host and pathogen. Key words: Macrophomina phaseolina MS-6, optical mapping, assembly improvement, assembly validation.