Cape gooseberry has coped with abiotic and biotic stresses such as prolonged waterlogging periods and vascular wilt in recent years. The aim of this study was to evaluate the influence of four waterlogging periods on stomatal conductance (g_s), leaf water potential (Ψ_wf), plant growth, leaf photosynthetic pigments, malondialdehyde (MDA) production, proline content and chlorophyll fluorescence parameters in cape gooseberry plants infected with Fusarium oxysporum f. sp. physali (Foph). Two-month-old ecotype “Colombia” plants were arranged in a completely randomized factorial design in eight treatments: plants without waterlogging (control), plants with waterlogging for 4, 6 and 8 d with and without Foph, respectively. The area under the disease progress curve was higher in inoculated plants subjected to 6 and 8 d of waterlogging (55.25 and 64.25) compared to inoculated plants but without waterlogging (45.25). The results also showed a lower plant growth, g_s, Ψ_wf, leaf photosynthetic pigments and chlorophyll fluorescence parameters (F_v/F_m, electron transport rate (ETR), Y (II) and qP) as waterlogging periods in plants with Foph increased. However, this group of plants showed a greater proline and malondialdehyde (MDA) accumulation and a higher NPQ. In conclusion, cape gooseberry shows a low acclimation to waterlogging conditions of more than 6 d in soils with Foph.

Exogenous glycine betaine (GB) or hydrogen peroxide (H₂O₂) application has not been explored to mitigate waterlogging stress in Andean fruit trees. The objective of this study was to evaluate foliar GB or H₂O₂ application on the physiological behavior of Cape gooseberry plants under waterlogging. Two separate experiments were carried out. In the first trial, the treatment groups were: (1) plants without waterlogging and with no foliar applications, (2) plants with waterlogging and without foliar applications, and (3) waterlogged plants with 25, 50, or 100 mM of H₂O₂ or GB, respectively. The treatments in the second trial were: (1) plants without waterlogging and with no foliar applications, (2) plants with waterlogging and without foliar applications, and (3) waterlogged plants with 100 mM of H₂O₂ or GB, respectively. In the first experiment, plants with waterlogging and with exogenous GB or H₂O₂ applications at a dose of 100 mM showed higher leaf water potential (−0.5 Mpa), dry weight (1.0 g), and stomatal conductance (95 mmol·m⁻²·s⁻¹) values. In the second experiment, exogenously supplied GB or H₂O₂ also increased the relative growth rate, and leaf photosynthesis mitigating waterlogging stress. These results show that short-term GB or H₂O₂ supply can be a tool in managing waterlogging in Cape gooseberry.

The objective of this work was to study the effect of foliar boric acid (BA) or sodium borate (SB) sprays on rice plants subjected to high daytime temperature. The established treatments were the following: i) seedlings with heat stress (40 °C) and treated with foliar SB sprays at three rates 25, 50 or 100 mg of SB L⁻¹, ii) seedlings with heat stress and with foliar BA sprays at three rates 25, 50 or 100 mg of BA L⁻¹, iii) seedlings without heat stress (28 °C) and foliar boron (B) sprays (absolute control-AB) and iv) seedlings with heat stress and any foliar B compounds treatments (HT), resulting a total of eight group of treatments (AB, HT, SB25, SB50, SB100, BA25, BA50 and BA100). The results showed that, in heat stress condition, foliar BA (25, 50 or 100 mg L⁻¹, respectively) or SB (50 mg L⁻¹) sprays significantly increased the values of net photosynthesis compared to untreated plants (19.7 µmol CO₂ m⁻² s⁻¹ with B 14.4 µmol CO₂ m⁻² s⁻¹ without B). The foliar B compounds sprays caused an increase on photochemical efficiency of PSII (0.76 with B vs. 0.72 without B) and lower oxidative damage (expressed as leaf malondialdehyde and proline production) in seedlings under heat stress. The results in the present study allow concluding that the use of B (BA or SB) can be considered as an agronomic strategy to mitigate the negative impact of heat stress in rice when high daytime stress periods are expected in rice areas.

This study aimed to assess the soil application of mixtures of biological control agents (BCAs) (Trichoderma virens and Bacillus velezensis) and organic additives (chitosan and burnt rice husk) on the physiological and biochemical behavior of cape gooseberry plants exposed to Fusarium oxysporum f. sp. physali (Foph) inoculum. The treatments with inoculated and non-inoculated plants were: (i) T. virens + B. velezensis (Mix), (ii) T. virens + B. velezensis + burnt rice husk (MixRh), (iii) T. virens + B. velezensis + chitosan (MixChi), and (iv) controls (plants without any mixtures). Plants inoculated and treated with Mix or MixChi reduced the area under the disease progress curve (AUDPC) (57.1) and disease severity index (DSI) (2.97) compared to inoculated plants without any treatment (69.3 for AUDPC and 3.2 for DSI). Additionally, these groups of plants (Mix or MixChi) obtained greater leaf water potential (~−0.5 Mpa) and a lower MDA production (~12.5 µmol g⁻² FW) than plants with Foph and without mixtures (−0.61 Mpa and 18.2 µmol g⁻² FW, respectively). The results suggest that MixChi treatments may be a promising alternative for vascular wilt management in cape gooseberry crops affected by this disease.

Vascular wilt caused by Fusarium oxysporum is the most limiting disease that affects cape gooseberry (Physalis peruviana L.) crops in Colombia. The use of synthetic elicitors for vascular wilt management is still scarce in Andean fruit species. The objective of the present study was to evaluate the effect and number of foliar applications of synthetic elicitors such as jasmonic acid (JA), salicylic acid (SA), brassinosteroids (BR), or a commercial resistance elicitor based on botanical extracts (BE) on disease progress and their effect on the physiology of cape gooseberry plants inoculated with F. oxysporum f. sp. physali. Groups of ten plants were separately sprayed once, twice, or three times with a foliar synthetic elicitor, respectively. Elicitor applications were performed at the following concentrations: JA (10 mL L⁻¹), SA (100 mg L⁻¹), BR (1 mL L⁻¹) and BE (2.5 mL of commercial product (Loker^®) L⁻¹). The results showed that three foliar BR, SA, or BE applications reduced the area under the disease progress, severity index, and vascular browning in comparison to inoculated plants without any elicitor spray. Three BR, SA, or BE sprays also favored stomatal conductance, water potential, growth (total dry weight and leaf area) and fluorescence parameters of chlorophyll compared with inoculated and untreated plants with no elicitor sprays. Three foliar sprays of SA, BR, or BE enhanced photosynthetic pigments (leaf total chlorophyll and carotenoid content) and proline synthesis and decreased oxidative stress in Foph-inoculated plants. In addition, the effectiveness of three foliar BR, SA, or BE sprays was corroborated by three-dimensional plot and biplot analysis, in which it can evidence that stomatal conductance, proline synthesis, and efficacy percentage were accurate parameters to predict Foph management. On the hand, JA showed the lowest level of amelioration of the negative effects of Foph inoculation. In conclusion, the use of the synthetic elicitors BR, SA, or BE can be considered as a tool complementary for the commercial management of vascular wilt in areas where this disease is a limiting factor.

Cape gooseberry (Physalis peruviana) fruit has gained recognition owing to its nutritional value and versatility to be consumed processed or as a fresh product. These characteristics have made it an important product in both national and international markets. One of the main limitations for this crop is Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. physalis, for which biological control is emerging as an alternative to conventional management with chemical synthesis products. However, information on the effect that biological control agents have on the growth and development of plants is scarce. In this research, the physiological response of cape gooseberry plants (stomatal conductance, leaf water potential, growth parameters, total chlorophyll, carotenoid, and proline and malondialdehyde contents) to the treatment with three potential biocontrol agents (BCAs) Trichoderma koningiopsis, Trichoderma virens, and Bacillus velezensis was determined. The study was conducted under greenhouse conditions; F. oxysporum was inoculated in the soil, and BCAs were soil drenched in the germination and transplanting stages. Plants inoculated with the pathogen and plants without inoculation were used as controls. It was found that the plants inoculated and treated with T. virens showed the lowest disease levels (area under the disease progress curve of 48.5 and disease severity index of 2.1). Additionally, they showed a lower water potential (−0.317 Mpa), a greater leaf area (694.7 cm²), and a higher stomatal conductance (110.3 mmol m⁻² s⁻¹) compared with the control. Consequently, it can be concluded that T. virens can be a good candidate for the management of Fusarium wilt in the cape gooseberry crop.