This study investigated the influence of heavy metals on bacterial community structure in a uranium mine. Soils from three differently polluted ditches (Yangchang ditch, Zhongchang ditch, and Sulimutang ditche) were collected from Zoige County, Sichuan province, China. Soil physicochemical properties and heavy metal concentrations were measured. Differences between bacterial communities were investigated using the high-throughput sequencing of the 16S rRNA genes. The obtained results demonstrated that bacterial richness index (Chao and Ace) were similar among three ditches, while the highest bacterial diversity index was detected in the severely contaminated soils. The compositions of bacterial communities varied among three examined sites, but Proteobacteria and Acidobacteria were abundant in all samples. Redundancy analysis revealed that soil organic matter, Cr and pH were the three major factors altering the bacterial community structure. Pearson correlation analysis indicated that the most significant correlations were observed between the contents of non-residual Cr and the abundances of bacterial genera, including Thiobacillus, Nitrospira, and other 10 genera. Among them, the abundances of Sphingomonas and Pseudomonas were significant and positively correlated with the concentrations of non-residual U and As. The results highlighted the factors influencing the bacterial community in uranium mines and contributed a better understanding of the effects of heavy metals on bacterial community structure by considering the fraction of heavy metals.

Post phytoremediation accumulation of heavy metals in plants is causing an environmental issue worldwide. In this study, we investigated the ability of eight different kinds of microorganisms to degrade and release heavy metals from heavy metal enriched ryegrass, including 5 species of bacteria (Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus-I, Bacillus pumilus-II and Bacillus cereus) and 3 of fungi (Phanerochaete chrysosporium, Trichoderma ressei and Pterula sp. strain QD-1), by growing them under uranium stress and assessing their ability to degrade biomass. After 30 days, the degradation ability of fungi was found better than that of bacteria, while the metal leaching ability of bacteria was found better. The highest degradation rate (upto 60%) was obtained by using P. chrysosporium, Pterula sp. strain QD-1 exhibited the best leaching rate for uranium (upto 77%). The overall degradation rate of lignin and cellulose and hemicellulose was found lower (40% and 60%, respectively). According to the antagonistic characteristics of microbes, we combined different dominant species, in which under optimal conditions the T2 combination (P. chrysosporium, T. reesei, and Pterula sp. strain QD-1 and B. subtilis) was able to degrade 80% of the ryegrass, 51% of lignin, 74% of cellulose and hemicellulose, releasing 78% of U, 90% of Pb and the releasing rate of other heavy metals was more than 95%. FTIR analysis showed the least degradation of lignin, while SEM-EDX analysis of the degradation residues displayed the microstructure of ryegrass being greatly damaged. Only a small amount of U was found in the residues of the researched combinations. This study provides efficient Microbial Combined Degradation Technology for heavy metal enriched biomass, which can effectively deal with heavy metal enriched plants, and provide a basis for the recovery and utilization of heavy metals, avoiding secondary pollution in the environment caused by this type of biomass.