Background and Aim: Ovariohysterectomy (OHE) is a common procedure for sterilization of female dogs. However, knowledge of changes in pain stress, oxidative stress, and total antioxidant power status before, during, and after OHE is limited. The objective of this experiment was to study the effect of duration on pain stress, oxidative stress, and total antioxidant power status in female dogs undergoing OHE. Materials and Methods: Seven female dogs were sterilized using the OHE method. Pain scores, hematological changes, and biochemical markers were investigated during pre-operative, 3 h after starting OHE, and on days 3, 7, 10, and 14 of an experimental period. Data were analyzed using one-way analysis of variance. Results: At 3 days after OHE, pain score was higher than on days 7-14 of the experimental period; percentage of neutrophil, 3 h after starting OHE, was higher than during pre-operative and on days 3-14 of the experimental period; percentage of lymphocyte on days 10-14 was lower than during pre-operative, 3 h after starting OHE, and on days 3-7 of the experimental period; neutrophil/lymphocyte ratio, 3 h after starting OHE, was higher than during pre-operative and on days 3-14 of the experimental period; plasma malondialdehyde on day 3 was higher than during pre-operative, 3 h after starting OHE, and on days 3-14 of the experimental period; and total antioxidant power on day 14 was higher than during pre-operative, 3 h after starting OHE, and on days 3-10 of the experimental period, respectively. Conclusion: This experiment indicated that OHE caused pain stress, oxidative stress, and reduction of total antioxidant power in female dogs. Finally, female dogs needed antioxidant for 7 days after OHE.

Effects of Dexamethasone on Physiological Changes and Productive Performance in Broilers

1. The objective of this study was to measure the effect of gallic acid on levels of ferric reducing antioxidant power, malondialdehyde, hydrogen peroxide, nitric oxide and the viability of broiler blood cells (BBCs) when exposed to high ambient temperature. 2. The BBCs were maintained at 41.5°C (control group, CG) or at ambient temperatures ranging from 41.5°C to 46°C. At 41.5°C to 46°C, BBCs were diluted with gallic acid at 0 (positive control group, PCG), 6.25, 12.5, 25 and 50 µmol, respectively. Ferric reducing antioxidant power, malondialdehyde, hydrogen peroxide, nitric oxide and viability of BBCs were investigated. 3. Hydrogen peroxide, malondialdehyde and nitric oxide for the CG was lower than PCG (P<0.05). However, the viability of CG was higher than PCG (P<0.05). At 41.5 to 46°C, malondialdehyde, hydrogen peroxide, and nitric oxide of BBCs diluted with gallic acid were lower compared to PCG (P<0.05). Viability of BBCs diluted with gallic acid was higher than PCG (P<0.05). 4. These results indicated that gallic acid could reduce the adverse oxidative effects of high ambient temperature on BBCs, with an optimum dilution rate of 12.5 µmol.