During the long migration from river habitats to the spawning ground, the Japanese eel undergoes sexual maturation. This spawning migration occurs concurrently with morphological changes, such as increases in eye size; however, the mechanisms by which sex steroids and their receptors influence these changes in peripheral tissues remain unclear. The aim of this study was to investigate changes in the eyes of female Japanese eels during sexual maturation, and our research focused on estrogen receptor (ER)α and ERβ transcripts. During ovarian development, the gonadosomatic index increased and yolk-laden oocytes developed rapidly. These changes occurred in conjunction with a steady increase in plasma levels of estradiol-17β (E₂). Concomitant increases in transcript levels of ERα and ERβ in eye, brain, pituitary, and ovary were also observed. Fluorescence in-situ hybridization analyses revealed that ERα and ERβ transcripts were present in the choriocapillary layer and photoreceptor layer of the eyes, and the analysis also revealed that their signals in these layers became stronger in mature females compared to those observed in immature females, suggesting that under the influence of gonadotropins, morphological changes in the eyes are regulated by E₂ through the activation of its receptors. In conclusion, E₂ plays a crucial role in physiological adaptations that occur in peripheral tissues during the spawning migration.

Marine environments provide a unique opportunity to blend offshore wind energy production and marine fishery activities as complementary technologies. This study investigated the morphological characteristics (length and weight) and biomass yield of seaweed (Undaria pinnatifida) in a model marine environment with mariculture within an offshore wind farm in southwestern Korea. The mean lengths in the first cultivation trials of U. pinnatifida sporophytes increased from 1.8 ± 0.1 cm in November 2021 to 120–170 cm in March 2022 (density, 39.8 plants m⁻¹; final wet weight, 98.6–249.1 g (mean 146.8 ± 20.4 g, n = 20 ind.); yield 5842 g m⁻¹). Further, for the second cultivation trial, the length of the sporophytes increased from 1.5 ± 0.1 cm in November 2021 to 120–150 cm in April 2022 (density, 49.3 plants m⁻¹; final wet weight, 83.0–251.6 g (mean 155.7 ± 19.0 g; n = 20 ind.); yield, 7676 g m⁻¹), and, owing to the increase in water temperature and light intensity due to seasonal changes around the offshore wind power farm, the second cultivation trials showed signs of chlorosis. Considering the environment, we judged seaweed growth to be normal. Therefore, when applying this model to grow U. pinnatifida, seasonal temperature changes, the purpose of the product, and the nutritional status of the open-sea area should be considered. These results may improve seaweed farming in offshore wind farms in the future.

Assessment of the clock genes, Period (Per) 1, Per2, Per3, and Cryptochrome (Cry) 2, Cry3, and Cry4, can help better understand eel spawning ecology. In this study, the circadian rhythm and moonlight effects of these clock genes in the eel retina and hypothalamus were analyzed. We examined clock gene expression patterns under 12 h light:12 h darkness (12L12D), constant darkness (DD), and constant light (LL) conditions; under short photoperiod (SP; 9L15D) and long photoperiod (LP; 15L9D), and during the new moon (NM) and full moon in male eels. Per2 expression increased after sunrise, Cry2, and Cry4 expression increased around sunset, and Per1, Per3, and Cry3 expression increased before sunrise. Under SP conditions, oscillations of retinal Per3 and Cry4, which did not occur under LP conditions, were generated. In addition, retinal Cry4 oscillation was generated under NM conditions. These results suggest that the retina of the eel may play an important role in regulating circadian rhythm, and migration is initiated by the synchronization of clock genes by moonlight, suggesting that photic signals are closely related to the migratory activity of the eel.

In this study, using Illumina sequencing, we sequenced first the complete mitochondrial genome (mitogenome) of two deep-sea eels, Histiobranchus bathybius and Simenchelys parasitica, collected from the East Mariana Basin in the Western Pacific Ocean. The complete length of the H. bathybius and S. parasitica mitogenomes were 16,696 and 16,687 bp, respectively, each containing 37 genes (13 protein-coding genes, 22 tRNA genes, and 2 ribosomal RNA genes). To enhance the accuracy of the identification of H. bathybius and S. parasitica, we performed a phylogenetic analysis of multiple deep-sea eels based on the mitochondrial DNA gene (cytochrome c oxidase subunit I [COI]) using the maximum likelihood method. Our phylogenetic tree analysis confirmed that the specimens collected in this study are congeneric species of H. bathybius and S. parasitica reported in previous studies. Based on these results, we report the first complete mitogenomes of H. bathybius and S. parasitica and a new record for the two species in the East Mariana Basin.

Fisheries and Aquatic Sciences