Artificial Intelligence (AI)-based systems and their increasingly common use have made it a ubiquitous technology; Machine Learning algorithms are present in streaming services, social networks, and in the health sector. However, implementing this emerging technology carries significant social and ethical risks and implications. Without ethical development of such systems, there is the potential for this technology to undermine people’s autonomy, privacy, and equity, even affecting human rights. Considering the approaches necessary for ethical development and effective governance of AI, such as ethical principles, guidelines, and technical tools, the question arises regarding the limitations of implementing these measures by the highly technical personnel involved in the process. In this context, we propose the creation of a typology that distinguishes the different stages of the AI life-cycle, the high-level ethical principles that should govern their implementation, and the tools with the potential to foster compliance with these principles, encompassing both technical and conceptual resources. In addition, this typology will include relevant information such as developmental level, related tasks, sectors, and language. Our research is based on a systematic review in which we identified 352 resources and tools. We expect this contribution to be valuable in promoting ethical AI development for developers and leaders who manage these initiatives. The complete typology and the comprehensive list of resources are available for consultation at https://ricardo-ob.github.io/tools4responsibleai.

This article presents improvements in the segmentation module, feature extraction module, and the classification module of a low-cost automated visual inspection (AVI) system for wood defect classification. One of the major drawbacks in the low-cost AVI system was the erroneous segmentation of clear wood regions as defects, which then introduces confusion in the classification module. To reduce this problem, we use the fuzzy min–max neural network for image segmentation (FMMIS). The FMMIS method grows boxes from a set of seed pixels, yielding ideally the minimum bounded rectangle for each defect present in the wood board image. Additional features with texture information are considered for the feature extraction module, and multi-class support vector machines are compared with multilayer perceptron neural networks in the classification module. Results using the FMMIS, additional features, and a pairwise classification support vector machine on a 550 test wood image set containing 11 defect categories show 91% of correct classification, which is significantly better than the original 75% of the low-cost AVI system. The use of computational intelligence techniques improved significantly the overall performance of the proposed automated visual inspection system for wood boards.

Plant defense responses to biotic stresses are complex biological processes, all governed by sophisticated molecular regulations. Induced systemic resistance (ISR) is one of these defense mechanisms where beneficial bacteria or fungi prime plants to resist pathogens or pest attacks. In ISR, the defense arsenal in plants remains dormant and it is only triggered by an infection, allowing a better allocation of plant resources. Our group recently described that the well-known beneficial bacterium Paraburkholderia phytofirmans PsJN is able to induce Arabidopsis thaliana resistance to Pseudomonas syringae pv. tomato (Pst) DC3000 through ISR, and that ethylene, jasmonate and salicylic acid are involved in this protection. Nevertheless, the molecular networks governing this beneficial interaction remain unknown. To tackle this issue, we analyzed the temporal changes in the transcriptome of PsJN-inoculated plants before and after being infected with Pst DC3000. These data were used to perform a gene network analysis to identify highly connected transcription factors. Before the pathogen challenge, the strain PsJN regulated 405 genes (corresponding to 1.8% of the analyzed genome). PsJN-inoculated plants presented a faster and stronger transcriptional response at 1-hour post infection (hpi) compared with the non-inoculated plants, which presented the highest transcriptional changes at 24 hpi. A principal component analysis showed that PsJN-induced plant responses to the pathogen could be differentiated from those induced by the pathogen itself. Forty-eight transcription factors were regulated by PsJN at 1 hpi, and a system biology analysis revealed a network with four clusters. Within these clusters LHY, WRKY28, MYB31 and RRTF1 are highly connected transcription factors, which could act as hub regulators in this interaction. Concordantly with our previous results, these clusters are related to jasmonate, ethylene, salicylic, acid and ROS pathways. These results indicate that a rapid and specific response of PsJN-inoculated plants to the virulent DC3000 strain could be the pivotal element in the protection mechanism.

Quorum-sensing systems play important roles in host colonization and host establishment of Burkholderiales species. Beneficial Paraburkholderia species share a conserved quorum-sensing (QS) system, designated BraI/R, that controls different phenotypes. In this context, the plant growth-promoting bacterium Paraburkholderia phytofirmans PsJN possesses two different homoserine lactone QS systems BpI.1/R.1 and BpI.2/R.2 (BraI/R-like QS system). The BpI.1/R.1 QS system was previously reported to be important to colonize and produce beneficial effects in Arabidopsis thaliana plants. Here, we analyzed the temporal variations of the QS gene transcript levels in the wild-type strain colonizing plant roots. The gene expression patterns showed relevant differences in both QS systems compared with the wild-type strain in the unplanted control treatment. The gene expression data were used to reconstruct a regulatory network model of QS systems in P. phytofirmans PsJN, using a Boolean network model. Also, we examined the phenotypic traits and transcript levels of genes involved in QS systems, using P. phytofirmans mutants in homoserine lactone synthases genes. We observed that the BpI.1/R.1 QS system regulates biofilm formation production in strain PsJN and this phenotype was associated with the lower expression of a specific extracytoplasmic function sigma factor ecf26.1 gene (implicated in biofilm formation) in the bpI.1 mutant strain.