Zoysiagrass (Zoysia Willd.) is an important warm season turfgrass that is grown in many parts of the world. Salt tolerance is an important trait in zoysiagrass breeding programs. In this study, a genetic linkage map was constructed using sequence-related amplified polymorphism markers and random amplified polymorphic DNA markers based on an F₁ population comprising 120 progeny derived from a cross between Zoysia japonica Z105 (salt-tolerant accession) and Z061 (salt-sensitive accession). The linkage map covered 1211 cM with an average marker distance of 5.0 cM and contained 24 linkage groups with 242 marker loci (217 sequence-related amplified polymorphism markers and 25 random amplified polymorphic DNA markers). Quantitative trait loci affecting the salt tolerance of zoysiagrass were identified using the constructed genetic linkage map. Two significant quantitative trait loci (qLF-1 and qLF-2) for leaf firing percentage were detected; qLF-1 at 36.3 cM on linkage group LG4 with a logarithm of odds value of 3.27, which explained 13.1% of the total variation of leaf firing and qLF-2 at 42.3 cM on LG5 with a logarithm of odds value of 2.88, which explained 29.7% of the total variation of leaf firing. A significant quantitative trait locus (qSCW-1) for reduced percentage of dry shoot clipping weight was detected at 44.1 cM on LG5 with a logarithm of odds value of 4.0, which explained 65.6% of the total variation. This study provides important information for further functional analysis of salt-tolerance genes in zoysiagrass. Molecular markers linked with quantitative trait loci for salt tolerance will be useful in zoysiagrass breeding programs using marker-assisted selection.

Soil pH is a key factor affecting the growth of blueberries. Understanding the response mechanism of blueberries to different pH values and selecting suitable evaluation indexes are the basis of breeding new blueberry cultivars with high pH tolerances. The effects of different soil pH treatments for 17 months on the plant growth, fruit yield, photosynthetic characteristics, and leaf microelement concentration of Vaccinium ashei Reade ‘Climax’ and V. corymbosum hybrid ‘Chaoyue No. 1′ were studied. Plant height, main stem diameter, branch number per plant, leaf dry weight, stem dry weight, root dry weight, and total dry weight decreased with increasing soil pH. With an increase in soil pH, the first flowering date, 50% flowering date, first ripening date, and 50% ripening date of the two cultivars were postponed, and the flower bud numbers per plant, the floret numbers per bud, and yield per plant showed a downward trend. Moreover, the fruit quality decreased, which was reflected in the increase in the titratable acid content (TA) and the decrease in the total soluble solids content (TSS) and the TSS:TA ratio in the high pH treatment. With increasing soil pH, the chlorophyll content index (CCI), maximal photochemical efficiency of the PSII (Fv/Fm), quantum photosynthetic yield of the PSII (Y(II)) and net photosynthetic rate (Pn) of the two cultivars showed a downward trend, and some microelement concentrations in the leaves were imbalanced. Under high pH treatment, ‘Chaoyue No. 1′ had a relatively higher plant biomass and fruit yield, so it had a stronger tolerance to high pH than ‘Climax’ did. More strongly acidified rhizosphere soil capacity, as well as higher CCI, Fv/Fm, Y(II), and Pn values were the main reasons for the high pH tolerance of ‘Chaoyue No. 1′. Compared with destructive biomass indicators such as plant weight, nondestructive indicators such as CCI, Fv/Fm, and Y(II) can be more valuable indicators for fast and accurate evaluation of blueberry tolerance to high pH at early stages of treatment.