Unlike the numerous works concerning the effect of management on the forest mycobiome, only a few studies have addressed how fungi from different trophic groups recover from natural and anthropogenic disturbances and develop structural features typical of unmanaged old-growth forests. Our objective is to compare the soil fungal assemblages represented by different functional/trophic groups in protected and managed stands located in European mixed forests dominated by Scots pine. Fungal communities were analyzed using high-throughput Illumina MiSeq sequencing of fungal internal transcribed spacer 1 (ITS1) amplicons. Formerly managed forest reserves (established around 50 years ago) and forests under standard forest management appeared to be similar in terms of total and mean species richness of all fungal operational taxonomic units (OTUs), as well as OTUs assigned to different functional trophic groups. Among the 599 recorded OTUs, 497 (83%) were shared between both management types, whereas 9.5% of taxa were unique to forest reserves and 7.5% were unique to managed stands. Ascomycota and Basidiomycota were the predominant phyla, comprising 88% of all identified fungi. The main functional components of soil fungal assemblages consisted of saprotrophic (42% fungal OTUs; 27% reads) and ectomycorrhizal fungi (16%; 47%). Two-way analysis of similarities (ANOSIM) revealed that both site and management strategy influenced the species composition of soil fungal communities, with site being a primary effect for saprotrophic and ectomycorrhizal fungi. Volume of coarse and very fine woody debris and soil pH significantly influenced the ectomycorrhizal fungal community, whereas saprotrophic fungi were influenced primarily by volume of coarse woody debris and soil nitrate concentration. Among the identified fungal OTUs, 18 red-listed fungal species were identified from both forest reserves and managed forests, comprising two ECM fungi and four saprotrophs from the category of endangered species. Our results suggest that the transformation of fungal diversity after cessation of forest management is rather slow, and that both forest reserves and managed forests help uphold fungal diversity.

Invasive tree species change biodiversity, nutrient cycles, and ecosystem services, and can turn native ecosystems into novel ecosystems determined by invaders. In the acclimatization and invasion of alien tree species, the crucial role is played by ectomycorrhizal (ECM) fungi. We tested ECM fungi associated with Quercus rubra and Carya trees that are alien to Europe. Quercus rubra is among the most invasive tree species in Europe, and the Carya species are not considered invasive. Both form ectomycorrhizal symbiosis, and in their native range in North America, coexist in oak-hickory forests. Six study stands were located in Kórnik Arboretum: three for Q. rubra and three for Carya trees. Ectomycorrhizal fungi were assessed by molecular identification of ECM roots. We identified 73 ECM fungal taxa of 23 ECM phylogenetic lineages. All identified ECM fungi were native to Europe. Similar richness but different composition of ECM taxa were found on Q. rubra and Carya roots. Phylogenetic lineages /tomentella-thelephora, /russula-lactarius, and /genea-humaria were most abundant on both Carya and Q. rubra roots. Lineages /tuber-helvella and /entoloma were abundant only on Carya, and lineages /pisolithus-scleroderma and /cortinarius were abundant only on Q. rubra roots. Analysis of similarities revealed a significant difference in ectomycorrhizal assemblages between invasive Q. rubra and non-invasive Carya. Highlights: (1) under common garden conditions, ECM taxa richness was similar on Q. rubra and Carya roots; (2) ECM taxa composition differed between invasive Q. rubra and non-invasive Carya; (3) high abundance of long-distance exploration type (lineages from Boletales) was on Q. rubra; and (4) high abundance of short-distance exploration type (e.g., /tuber-helvella) was on Carya.