A cross-disciplinary (sedimentological, geochemical, micropalaeontological and archaeological) examination of 12 continuous cores, up to 20m long, integrated with stratigraphical, geomorphological and historical investigations, allows for reliable delineation of the middle-late Holocene environmental evolution in the Pisa old town area, with special emphasis on the Etruscan age transition. Depositional facies were identified through integration of sedimentological and micropalaeontological (benthic foraminifers, ostracods, phytoclasts and palynomorphs) data, while sediment dispersal patterns were reconstructed on the basis of geochemical analyses. Facies architecture was chronologically constrained by combined archaeological and radiocarbon dating. The turnaround from early Holocene, transgressive conditions to the ensuing (middle-late Holocene) phase of sea-level highstand is witnessed by a prominent shallowing-upward succession of lagoonal, paludal and then poorly drained floodplain deposits supplied by two river systems (Arno and Serchio). This 'regressive' trend, reflecting coastal progradation under nearly stable sea-level conditions, was interrupted by widespread swamp development close to the Iron-Etruscan age transition. The expansion of vast, low-lying paludal areas across the alluvial plain was mostly induced by the intricate, short-term evolution of the meandering Arno and Serchio river systems. These changes in the fluvial network, which occurred during a period of variable climate conditions, strongly influenced the early Etruscan culture (7th-5th century BC) in terms of human settlement and society behaviour. Conversely, a strong impact of human frequentation on depositional environments is observed at the transition to the Roman age (from the 1st century BC onwards), when the wetlands were drained and the modern alluvial plain started to form. The palaeoenvironmental reconstruction fits in with the original geographical descriptions mentioned in Strabo's Chronicles, and provides chronologically constrained data of fluvial evolution from the Pisa old town area. © 2013 Elsevier Ltd and INQUA