A shipboard study was conducted aboard the cruise ship Coral Princess during a scheduled cruise from San Pedro, CA, USA to Vancouver, British Columbia, Canada. The investigation involved three members of the global TestNet group, with experience in certification testing of ballast water treatment systems (BWTS) designed to eliminate entrained invasive species. A UV-based ballast water treatment system had been employed aboard the vessel for more than 10 years. A variety of established and experimental assessment techniques were employed, both aboard the ship and following shipment of samples via road (5 days) and air (7 days) to remote laboratories. The study was designed to compare the performance of different techniques in assessing BWTS compliance with international regulations, and to test the feasibility of compliance assessment by Port State Control internationally using different laboratories. Overall, biological end-points showed effective treatment of ballast water as judged by the percentage removal (mortality) of organisms in treated samples. Sample transport indicated generally good potential for ‘off-site’ sample analysis and displayed a possible latent effect of treatment as judged by a decline in photosynthetic yield associated with delayed analysis.

The unanimous adoption of the International Convention for the Control of Ballast Water and Sediments by the member states of the International Maritime Organization on 13 February 2004 was seen at...

The damaging introduction of invasive species continues unabated fourteen years after the adoption of the IMO Ballast Water Management Convention (BWMC). The Convention came into force in September, 2017, 13 years after its adoption. Qualifying commercial vessels will need to comply with international discharge regulations and must now contend with complex regulations, uncertain Ballast Water Treatment System performance and potentially inconsistent enforcement internationally. Part of the dilemma rests with the stringent standards which often take days to determine. When certification testing is superseded by rapid Port State Control (PSC) compliance assessment during a port visit, questions arise regarding the logistics of sampling/analysis and the functionality of the BWTS. Reflecting doubts about the performance of installed BWTS IMO have proposed an interim Experience Building Phase (EBP) wherein BWTS non-compliance would not be penalised if operated in good faith. This paper presents a rationale for a robust global programme to test the efficacy of BWTS through Port State Control, using the U.K. as a ‘Case Study’. Rapid prognostic testing during port visits would be employed using technologies certified by regulatory agencies. Such indicative testing would not necessarily address every single aspect of international standards, but would represent robust compliance assessment, that would provide feedback data vital to the improvement of BWTS. Abbreviations: AIS: Aquatic Invasive Species; BWMC: Ballast Water Management Convention; BWTS: Ballast Water Treatment System; CFC: Chlorofluorocarbons; EBP: (IMO) Experience Building Phase; FDOM: Fluorescent Dissolved Organic Matter; HC: Hydrocarbons; HCF: Hydrofluorocarbons; HCFC: Hydrochlorofluorocarbons; IMO: International Maritime Organization; IOPP: International Oil Pollution Prevention; MARPOL: Marine Pollution (Convention); MEPC: IMO Marine Environmental Protection Committee; NO_x: Nitrogen oxides; SERC: Smithsonian Environmental Research Center, Edgewater, Maryland, U.S.A.

Trials were conducted aboard the tanker Seabulk Mariner to test a natural product, SeaKleen®, as a biocide controlling non‐indigenous populations of plankton and bacteria in ballast water. SeaKleen® was dosed into matched ballast tanks at two different concentrations, 0.8 mg L⁻¹ active ingredient (a.i.) and 1.6 mg L⁻¹ a.i. during ballasting off the Oregon coast during a three‐day passage to Prince William Sound, Alaska. Live organism counts from treated ballast water were compared with those from untreated (control tank) water collected from the same source location. Shipboard chemical analyses were made to verify dose and quantify chemical degradation and residuals following dilution. Results indicated that both SeaKleen® doses resulted in complete zooplankton and phytoplankton mortality and that the higher dose (1.6 mg L⁻¹ a.i.) caused a two‐log removal of culturable bacteria over a 92 h grow‐out period. Spectrophotometry confirmed initial dosing to within 5% of nominal values. Shipboard bioassays were conducted using larval fish (Cyprinodon variegatus), brine shrimp (Artemia salina) and the bioluminescent dinoflagellate Pyrocystis lunula. Exposure of the test organisms to water drawn from treated ballast tanks 48 h after SeaKleen® was added to the tanks resulted in 100% mortalities in Cyprinodon and Pyrocystis at both doses. Corresponding mortalities for Artemia larvae were 100% and 60% for high and low SeaKleen® doses, respectively. Toxicity testing of treated water, subjected to varying dilutions, indicated that residual toxicity to even the most sensitive organisms would be eliminated once the discharge had dispersed beyond 100 feet from the vessel.

Invasive aquatic species can have damaging effects on fisheries and aquaculture through significant, and irreversible, effects on biodiversity. Human health may also be affected. To combat this threat the International Maritime Organization (IMO) Convention for Ballast Water and Sediments (BWMC) came into force in September 2017. U.S. Federal and IMO ballast water standards for discharged organisms stipulate discharge limits for different size classes of organisms. Several studies including recent trials aboard Great Lakes freighters have shown that many phytoplankton found in ballast water do not fall into the regulated 10–50 µM size class. Such issues illustrate the need for new methods of assessing microorganism populations that will supersede laborious microscopy requiring rare technical expertise. Recent progress has been made in the use of DNA (deoxyribose nucleic acid)-based methods as a means of identifying the appearance of invasive species in aquatic environments. A significant advance has been the development of high throughput sequencing (HTS), which has expanded DNA barcoding, relating to an individual organism, into second generation sequencing (metabarcoding), capable of mapping whole populations of organisms in an environmental sample. Several recent studies of HTS in ships’ ballast water, have shown that the technique has the capacity for detecting potentially harmful taxonomic groups and is capable of differentiating among water from different sources. The current study was undertaken to investigate the suitability (or otherwise) of HTS as a tool for ballast water management. Possible applications include improved risk assessment relating to invasive species. Feasibility for indicative testing for ballast water treatment efficacy was also addressed. However, pending analysis of treated samples, the current study was confined to a comparison of HTS and microscope counts in untreated samples. A correlation of visual and molecular taxonomic assignments of microorganisms found in the ballast water from different ports and during different seasons indicated that such a comparison was best conducted at Family level, although Principal Components Analysis showed that the two methods differed qualitatively among major taxonomic groups.

Tests of the efficacy of a combination filtration/ultraviolet (UV) ballast water treatment (BWT) system were conducted aboard the MV Coral Princess during normal operations from Victoria, British Columbia, through the Panama Canal, to Fort Lauderdale, Florida. Trials were designed to assist the maritime industry in the deployment of effective BWT systems and to inform regulatory agencies on the feasibility of testing procedures and end-point selection for compliance standards. Results showed treatment efficacy at least equivalent to ballast water exchange, although adjustment of current published standards was indicated.