K-promoted Mo-based catalysts showed great promise for the hydrogenation of CS₂ to methyl mercaptan (CH₃SH). However, the research on the synergistic effect of K and Mo, and the active site of CS₂ hydrogenation to CH₃SH were unexplored widely. To solve this problem, the synergistic effect of K and Mo in the K-promoted Mo-based catalysts for CS₂ hydrogenation to prepare CH₃SH was investigated. The mesoporous alumina was the support and loaded the active components potassium and molybdenum to prepare the catalyst. The results suggested that the active components K and Mo can not only cooperatively regulate the acid-base sites on the catalyst surface, but also stabilize the molybdate species at +5 valence during the reduction process and increase the Mo unsaturated coordination sites. Combined with the results of the catalytic activity evaluation, indicating that the main active site of the catalysts is the weak Lewis acid-base site, and the strong acidic site and strong alkaline site are not conducive to the formation of CH₃SH. Moreover, the possible catalytic mechanism of CS₂ hydrogenation to CH₃SH on the weak Lewis acid-base sites of the catalysts was proposed. The research results of this paper can provide an experimental basis and theoretical guidance for the design of high-performance CH₃SH synthesis catalyst and further mechanism research.

We explored the antimicrobial activity of sertraline on Listeria monocytogenes and further investigated the effects of sertraline on biofilm formation and the virulence gene expression of L. monocytogenes. The minimum inhibitory concentration and minimum bactericidal concentration for sertraline against L. monocytogenes were in the range of 16–32 μg/mL and 64 μg/mL, respectively. Sertraline-dependent damage of the cell membrane and a decrease in intracellular ATP and pH_in in L. monocytogenes were observed. In addition, sertraline reduced the biofilm formation efficiency of the L. monocytogenes strains. Importantly, low concentrations (0.1 μg/mL and 1 μg/mL) of sertraline significantly down-regulated the expression levels of various L. monocytogens virulence genes (prfA, actA, degU, flaA, sigB, ltrC and sufS). These results collectively suggest a role of sertraline for the control of L. monocytogenes in the food industry.