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Pyrenopeziza brassicae causes leaf spot disease of Brassicaceae in Europe/Oceania (lineage 1) and US (lineage 2). In Europe, fungicides currently used for disease management are sterol 14α-demethylase (CYP51) inhibitors (azoles), quinone outside inhibitors (QoIs) and succinate dehydrogenase inhibitors (SDHIs); methyl benzimidazole carbamates (MBCs) are no longer applied. In this study, in vitro screening revealed European populations (collected 2018–2020) had shifted towards decreased azole sensitivity, but the US population (2014–2016) was highly sensitive. Genotyping revealed CYP51 substitutions G460S or S508T were prevalent in European populations, often with a CYP51 promoter insert. Compared to wildtype CYP51 isolates, those with G460S plus an insert (44/46/151/210/302 bp) were c.25–32-fold and c.50-fold less sensitive to tebuconazole and prochloraz, respectively; those with S508T plus an insert (44/46/151/233 bp) were c.9–15-fold and c.25–40-fold less sensitive to tebuconazole and prochloraz, respectively. Selection for G460S (quantified via pyrosequencing) under different fungicide regimes was investigated in UK field trials, but G460S levels were high (c.76%) before treatment, so further selection during the trials was unclear. Despite the high G460S frequency and low disease pressure, yield data indicated measurable benefit for both azole- and non-azole-based programmes. In vitro screening against the MBC carbendazim showed European populations were predominantly moderately resistant/resistant; the US population was sensitive. European and US populations were sensitive to QoI (pyraclostrobin) and SDHI (penthiopyrad) fungicides. Results support an azole plus QoI/SDHI mixing partner for robust disease control and decreased risk of resistance, with continued sensitivity monitoring to ensure optimal strategies are deployed.
Kevin M. King; Diana E. Bucur; Faye Ritchie; Nichola J. Hawkins; Agata M. Kaczmarek; Yabing Duan; Steven Kildea; Jonathan S. West; Bart A. Fraaije. Fungicide resistance status and chemical control options for the brassica pathogen Pyrenopeziza brassicae. Plant Pathology 2021, 1 .
AMA StyleKevin M. King, Diana E. Bucur, Faye Ritchie, Nichola J. Hawkins, Agata M. Kaczmarek, Yabing Duan, Steven Kildea, Jonathan S. West, Bart A. Fraaije. Fungicide resistance status and chemical control options for the brassica pathogen Pyrenopeziza brassicae. Plant Pathology. 2021; ():1.
Chicago/Turabian StyleKevin M. King; Diana E. Bucur; Faye Ritchie; Nichola J. Hawkins; Agata M. Kaczmarek; Yabing Duan; Steven Kildea; Jonathan S. West; Bart A. Fraaije. 2021. "Fungicide resistance status and chemical control options for the brassica pathogen Pyrenopeziza brassicae." Plant Pathology , no. : 1.
A conserved kinase domain and phosphoryl group receiver domain at the C-terminus and poly-HAMP domains at the N-terminus comprise the structural components of the group III HK which was considered as a potential antifungal target. However, the roles of individual domains in the function of group III HKs have rarely been dissected in fungi. In this study, we dissected the roles of individual domains to better understand the function of Sshk1p, a group III HK from Sclerotinia sclerotiorum. The results suggest that individual domains play different roles in the functionality of Sshk1p and are implicated in the regulation of mycelial growth, sclerotia formation, pathogenicity. And the mutants of each domain in Sshk1 showed significantly increased sensitivity to hyperosmotic stress. However, the mutants of each domain in Sshk1 showed high resistance to fludioxonil and dimethachlon which suggested that all nine domains of Sshk1p were indispensable for susceptibility to fludioxonil and dimethachlon. Moreover, deletion of each individual domain in Sshk1 cancelled intracellular glycerol accumulation and increased SsHog1p phosphorylation level triggered by NaCl and fludioxonil, suggesting that all the domains of Sshk1 were essential for Sshk1-mediated SsHog1p phosphorylation and subsequent polyol accumulation in response to fludioxonil and hyperosmotic stress.
Tao Li; Qian Xiu; Qiao Wang; Jianxin Wang; Yabing Duan; Mingguo Zhou. Functional dissection of individual domains in group III histidine kinase Sshk1p from the phytopathogenic fungus Sclerotinia sclerotiorum. Pesticide Biochemistry and Physiology 2021, 178, 104914 .
AMA StyleTao Li, Qian Xiu, Qiao Wang, Jianxin Wang, Yabing Duan, Mingguo Zhou. Functional dissection of individual domains in group III histidine kinase Sshk1p from the phytopathogenic fungus Sclerotinia sclerotiorum. Pesticide Biochemistry and Physiology. 2021; 178 ():104914.
Chicago/Turabian StyleTao Li; Qian Xiu; Qiao Wang; Jianxin Wang; Yabing Duan; Mingguo Zhou. 2021. "Functional dissection of individual domains in group III histidine kinase Sshk1p from the phytopathogenic fungus Sclerotinia sclerotiorum." Pesticide Biochemistry and Physiology 178, no. : 104914.
Fusarium graminearum, causal agent of Fusarium head blight (FHB), causes a huge economic loss. No information is available on the activity of quinofumelin, a novel quinoline fungicide, against F. graminearum or other phytopathogens. In this study, we used mycelial growth and spore germination inhibition methods to determine the inhibitory effect of quinofumelin against F. graminearum in vitro. The results indicated that quinofumelin excellently inhibited mycelial growth and spore germination of F. graminearum, with the average EC50 values of 0.019 ± 0.007 μg/mL and 0.087 ± 0.024 μg/mL, respectively. In addition, we found that quinofumelin could significantly decrease deoxynivalenol (DON) production and inhibit the expression of DON-related gene TRI5 in F. graminearum. Furthermore, we found that quinofumelin could disrupt the formation of Fusarium toxisome, a structure for producing DON. Western blot analysis demonstrated that the translation level of TRI1, a marker gene for Fusarium toxisome, was suppressed by quinofumelin. The protective and curative assays indicated that quinofumelin had an excellent control efficiency against F. graminearum on wheat coleoptiles. Taken together, quinofumelin exhibits not only an excellent antifungal activity on mycelial growth and spore germination, but also could inhibit DON biosynthesis in F. graminearum. The findings provide a novel candidate for controlling FHB caused by F. graminearum.
Qian Xiu; Lianyu Bi; Haorong Xu; Tao Li; Zehua Zhou; Zhongke Li; Jianxin Wang; Yabing Duan; Mingguo Zhou. Antifungal Activity of Quinofumelin against Fusarium graminearum and Its Inhibitory Effect on DON Biosynthesis. Toxins 2021, 13, 348 .
AMA StyleQian Xiu, Lianyu Bi, Haorong Xu, Tao Li, Zehua Zhou, Zhongke Li, Jianxin Wang, Yabing Duan, Mingguo Zhou. Antifungal Activity of Quinofumelin against Fusarium graminearum and Its Inhibitory Effect on DON Biosynthesis. Toxins. 2021; 13 (5):348.
Chicago/Turabian StyleQian Xiu; Lianyu Bi; Haorong Xu; Tao Li; Zehua Zhou; Zhongke Li; Jianxin Wang; Yabing Duan; Mingguo Zhou. 2021. "Antifungal Activity of Quinofumelin against Fusarium graminearum and Its Inhibitory Effect on DON Biosynthesis." Toxins 13, no. 5: 348.
Deoxynivalenol (DON) is a vital virulence factor of Fusarium graminearum, which causes Fusarium head blight (FHB). We recently found that validamycin A (VMA), an aminoglycoside antibiotic, can be used to control FHB and inhibit DON contamination, but its molecular mechanism is still unclear. In this study, we found that both neutral and acid trehalase (FgNTH and FgATH) are the targets of VMA in F. graminearum, and the deficiency of FgNTH and FgATH reduces the sensitivity to VMA by 2.12‐ and 1.79‐fold, respectively, indicating that FgNTH is the main target of VMA. We found FgNTH is responsible for vegetative growth, FgATH is critical to sexual reproduction, and both of them play an important role in conidiation and virulence in F. graminearum. We found that FgNTH resided in the cytoplasm, affected the localization of FgATH, and positively regulated DON biosynthesis; however, FgATH resided in vacuole and negatively regulated DON biosynthesis. FgNTH interacted with FgPK (pyruvate kinase), a key enzyme in glycolysis, and the interaction was reduced by VMA; the deficiency of FgNTH affected the localization of FgPK under DON induction condition. Strains with a deficiency of FgNTH were more sensitive to demethylation inhibitor (DMI) fungicides. FgNTH regulated the expression level of FgCYP51A and FgCYP51B by interacting with FgCYP51B. Taken together, VMA inhibits DON biosynthesis by targeting FgNTH and reducing the interaction between FgNTH and FgPK, and synergizes with DMI fungicides against F. graminearum by decreasing FgCYP51A and FgCYP51B expression.
Chuanhong Bian; Yabing Duan; Qian Xiu; Jueyu Wang; Xian Tao; Mingguo Zhou. Mechanism of validamycin A inhibiting DON biosynthesis and synergizing with DMI fungicides against Fusarium graminearum. Molecular Plant Pathology 2021, 22, 769 -785.
AMA StyleChuanhong Bian, Yabing Duan, Qian Xiu, Jueyu Wang, Xian Tao, Mingguo Zhou. Mechanism of validamycin A inhibiting DON biosynthesis and synergizing with DMI fungicides against Fusarium graminearum. Molecular Plant Pathology. 2021; 22 (7):769-785.
Chicago/Turabian StyleChuanhong Bian; Yabing Duan; Qian Xiu; Jueyu Wang; Xian Tao; Mingguo Zhou. 2021. "Mechanism of validamycin A inhibiting DON biosynthesis and synergizing with DMI fungicides against Fusarium graminearum." Molecular Plant Pathology 22, no. 7: 769-785.
The high osmolarity glycerol (HOG) pathway, comprising a two-component system and the Hog1 mitogen-activated protein kinase (MAPK) cascade, plays a pivotal role in eukaryotic organisms. Previous studies suggested that the biological functions of some key genes in the HOG pathway varied in filamentous fungi. In this study, we characterized a putative MAPK kinase kinase gene, Ssos4, in Sclerotinia sclerotiorum, which encoded a phosphotransferase in the MAPK cascade. Compared with the wild-type progenitor HA61, the deletion mutant ∆Ssos4-63 exhibited impaired mycelial growth, sclerotia formation, increased hyphal branches, and decreased virulence. The deficiencies of the deletion mutant ∆Ssos4-63 were recovered when the full-length Ssos4 gene was complemented. Deletion of Ssos4 increased the sensitivity to osmotic stresses and cell wall agents and the resistance to fludioxonil and dimethachlon. Intracellular glycerol accumulation was not induced in the deletion mutant ∆Ssos4-63 when treated with fludioxonil and NaCl and the phosphorylation of SsHog1 was also cancelled by the deletion of Ssos4. Consistent with the glycerol accumulation and increased expression levels of SsglpA and Ssfps1, controlling glycerol synthesis and close of glycerol channel under hyperosmotic stress, respectively, were detected in the wild-type strain HA61 but not in the deletion mutant ∆Ssos4-63. Moreover, the relative expression level of Sshog1 significantly decreased, whereas the expression level of Ssos5 increased in the deletion mutant ∆Ssos4-63. These results indicated that Ssos4 played important roles in mycelial growth and differentiation, sclerotia formation, virulence, hyperosmotic adaptation, fungicide sensitivity, and the phosphorylation of SsHog1 in S. sclerotiorum.
Tao Li; Miss Qian Xiu; Jianxin Wang; Yabing Duan; Mingguo Zhou. A Putative MAPK Kinase Kinase Gene Ssos4 is Involved in Mycelial Growth, Virulence, Osmotic Adaptation, and Sensitivity to Fludioxonil and is Essential for SsHog1 Phosphorylation in Sclerotinia sclerotiorum. Phytopathology® 2021, 111, 521 -530.
AMA StyleTao Li, Miss Qian Xiu, Jianxin Wang, Yabing Duan, Mingguo Zhou. A Putative MAPK Kinase Kinase Gene Ssos4 is Involved in Mycelial Growth, Virulence, Osmotic Adaptation, and Sensitivity to Fludioxonil and is Essential for SsHog1 Phosphorylation in Sclerotinia sclerotiorum. Phytopathology®. 2021; 111 (3):521-530.
Chicago/Turabian StyleTao Li; Miss Qian Xiu; Jianxin Wang; Yabing Duan; Mingguo Zhou. 2021. "A Putative MAPK Kinase Kinase Gene Ssos4 is Involved in Mycelial Growth, Virulence, Osmotic Adaptation, and Sensitivity to Fludioxonil and is Essential for SsHog1 Phosphorylation in Sclerotinia sclerotiorum." Phytopathology® 111, no. 3: 521-530.
Glucose-6-phosphate isomerase (GPI) is ubiquitous in most organisms, catalyzing the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. In this study, we investigated biological and genetic functions of FgGPI in the phytopathogen Fusarium graminearum. We found that hyphal growth, conidial germination, and septa formation were significantly inhibited in FgGPI deletion mutant ∆FgGPI. FgGPI was also positively associated with glucose metabolism, ATP biosynthesis, and carbon source utilization. In addition, pyruvate production, deoxynivalenol (DON) biosynthesis, and virulence were reduced in ∆FgGPI. A coimmunoprecipitation assay demonstrated that FgGPI interacts with Fgβ2. More importantly, the coimmunoprecipitation assay showed that carbendazim-resistant substitutions in β2 tubulin could reduce the interaction intensity between FgGPI and Fgβ2, thereby increasing FgGPI expression and accelerating DON biosynthesis in carbendazim-resistant strains. Taken together, our work revealed the indispensable role of FgGPI in fungal developmental processes, DON biosynthesis, and pathogenicity in F. graminearum.
Zehua Zhou; Jie Zhang; Fei Lu; Yabing Duan; Mingguo Zhou. Glucose-6-Phosphate Isomerase FgGPI, a β2 Tubulin-Interacting Protein, Is Indispensable for Fungal Development and Deoxynivalenol Biosynthesis in Fusarium graminearum. Phytopathology® 2021, 111, 531 -540.
AMA StyleZehua Zhou, Jie Zhang, Fei Lu, Yabing Duan, Mingguo Zhou. Glucose-6-Phosphate Isomerase FgGPI, a β2 Tubulin-Interacting Protein, Is Indispensable for Fungal Development and Deoxynivalenol Biosynthesis in Fusarium graminearum. Phytopathology®. 2021; 111 (3):531-540.
Chicago/Turabian StyleZehua Zhou; Jie Zhang; Fei Lu; Yabing Duan; Mingguo Zhou. 2021. "Glucose-6-Phosphate Isomerase FgGPI, a β2 Tubulin-Interacting Protein, Is Indispensable for Fungal Development and Deoxynivalenol Biosynthesis in Fusarium graminearum." Phytopathology® 111, no. 3: 531-540.
Sclerotinia sclerotiorum is a devastating plant pathogen with a broad host range and worldwide distribution. The application of chemical fungicides is a primary strategy for controlling this pathogen. However, under the high selective pressure of chemical fungicides, fungicide resistance has emerged and gradually increased, resulting in the failure to control S. sclerotiorum in the field. Quinofumelin is a novel quinoline fungicide, but its antifungal activities against plant pathogens have been rarely reported. Here, we determined the antifungal activity of quinofumelin against S. sclerotiorum in vitro and in planta. The EC50 values ranged from 0.0004 to 0.0059 μg mL-1 with a mean EC50 of 0.0017 ± 0.0009 μg mL-1 and were normally distributed (P=0.402). In addition, no cross-resistance was observed between quinofumelin and other fungicides, dimethachlone, boscalid, or carbendazim, which are commonly used to manage S. sclerotiorum. Quinofumelin did not affect glycerol and oxalic acid production of either carbendazim-sensitive or -resistant isolates. Moreover, quinofumelin exhibited excellent protective, curative, and translaminar activity against S. sclerotiorum on oilseed rape leaves. Protective activity was higher than curative activity. Interestingly, quinofumelin inhibited the formation of the infection cushion in S. sclerotiorum, which may contribute to the control efficacy of quinofumelin against S. sclerotiorum in the field. Our findings indicate that quinofumelin has excellent control efficacy against S. sclerotiorum in vitro and in planta as compared with the currently extensively used fungicides and could be used to manage carbendazim- and dimethachlone-resistance in S. sclerotiorum in the field.
Miss Xian Tao; Huahua Zhao; Miss Haorong Xu; Zhongke Li; Jian-Xin Wang; Xiu-Shi Song; Mingguo Zhou; Yabing Duan. Antifungal activity and biological characteristics of the novel fungicide quinofumelin against Sclerotinia sclerotiorum. Plant Disease 2021, 1 .
AMA StyleMiss Xian Tao, Huahua Zhao, Miss Haorong Xu, Zhongke Li, Jian-Xin Wang, Xiu-Shi Song, Mingguo Zhou, Yabing Duan. Antifungal activity and biological characteristics of the novel fungicide quinofumelin against Sclerotinia sclerotiorum. Plant Disease. 2021; ():1.
Chicago/Turabian StyleMiss Xian Tao; Huahua Zhao; Miss Haorong Xu; Zhongke Li; Jian-Xin Wang; Xiu-Shi Song; Mingguo Zhou; Yabing Duan. 2021. "Antifungal activity and biological characteristics of the novel fungicide quinofumelin against Sclerotinia sclerotiorum." Plant Disease , no. : 1.
Phenazine-1-carboxylic acid (PCA), a member of phenazines secreted by microorganisms, inhibits the growth of many bacteria and fungi. Xanthomonas campestris pv. campestris is the causal agent of black rot, the most important disease of cruciferous crops worldwide, and is more tolerant to PCA than other Xanthomonas species. Previous studies reported that reactive oxygen species (ROS) scavenging ability is involved in regulating the PCA tolerance of Xanthomonas species. Additionally, the cytochrome c maturation (CCM) system has been found to play a more important role in tolerance to phenazines than the ROS scavenging system. In this study, a highly PCA-sensitive insertion mutant of X. campestris pv. campestris, X-5, was identified and studied. The insertion site of X-5 was found to be in tatB gene (XC_4183), which encodes a subunit of the twin-arginine translocation (TAT) complex. Disruption of the three genes of TAT pathway resulted in decreased biological fitness and reduced tolerance to phenazines in comparison with the wild-type strain 8004. These results imply that the tolerance mechanism of the TAT pathway to phenazines is related to the CCM system, but not due to the ROS scavenging system. Furthermore, respiration-related characteristic tests and peptide analysis suggested that disruption of the TAT complex causes a defect in the cytochrome bc1 complex, which may be involved in the tolerance to phenazines. In summary, this study sheds new light on the critical role of the TAT pathway in influencing the fitness and phenazines tolerance of Xanthomonas species.
Jian Wu; Xiayan Pan; Shu Xu; Yabing Duan; Jueyu Wang; Jianxin Wang; Tongchun Gao; Yong Zhang; Mingguo Zhou. A Defect in the Twin-Arginine Translocation Pathway Decreases the Tolerance of Xanthomonas campestris pv. campestris to Phenazines. Phytopathology® 2020, 1 -03.
AMA StyleJian Wu, Xiayan Pan, Shu Xu, Yabing Duan, Jueyu Wang, Jianxin Wang, Tongchun Gao, Yong Zhang, Mingguo Zhou. A Defect in the Twin-Arginine Translocation Pathway Decreases the Tolerance of Xanthomonas campestris pv. campestris to Phenazines. Phytopathology®. 2020; ():1-03.
Chicago/Turabian StyleJian Wu; Xiayan Pan; Shu Xu; Yabing Duan; Jueyu Wang; Jianxin Wang; Tongchun Gao; Yong Zhang; Mingguo Zhou. 2020. "A Defect in the Twin-Arginine Translocation Pathway Decreases the Tolerance of Xanthomonas campestris pv. campestris to Phenazines." Phytopathology® , no. : 1-03.
Cucumber target spot, caused by Corynespora cassiicola, is a devastating fungal disease in greenhouses in China. Lack of resistant cultivars and unscientific use of fungicides aggravated the difficulty to manage this disease. In recent years, resistance of C. cassiicola to benzimidazoles, quinone outside inhibitors, and succinate dehydrogenase inhibitors has occurred in China. Here, we tested the fluazinam sensitivity distribution of 79 C. cassiicola isolates from different provinces in China based on mycelial growth inhibition. The EC50 values of fluazinam ranged from 0.1002 to 0.3129 µg/ml with a mean of 0.2136 ± 0.0495 µg/ml, and the sensitivity frequency was normally distributed (P = 0.2083, Shapiro–Wilk test). Meanwhile, the EC50 values for spore germination inhibition ranged from 0.0992 to 0.2278 µg/ml with a mean of 0.1499 ± 0.0504 µg/ml. This indicated that fluazinam exhibited an excellent in vitro fungicidal activity on both mycelial growth and spore germination. In addition, fluazinam also exhibited a good in planta control efficacy on detached cucumber leaves in the protective and curative assays. Moreover, the biological and physiological characteristics of C. cassiicola as affected by fluazinam were determined. Fluazinam not only significantly inhibited respiration and adenosine triphosphate production but also caused the increase of cell membrane permeability and the dysfunctions of cellular homeostasis. Interestingly, we found that fluazinam especially damaged vacuole structures, causing the redistribution of vacuole substances. Taken together, our findings provide not only essential references for resistance management of C. cassiicola but also interesting insights for further revealing the action mode of fluazinam against plant pathogens.
Tao Li; Miss Qian Xiu; Jie Zhang; Jian Xin Wang; Ya Bing Duan; Ming Guo Zhou. Pharmacological Characteristics and Efficacy of Fluazinam Against Corynespora cassiicola, Causing Cucumber Target Spot in Greenhouses. Plant Disease 2020, 104, 2449 -2454.
AMA StyleTao Li, Miss Qian Xiu, Jie Zhang, Jian Xin Wang, Ya Bing Duan, Ming Guo Zhou. Pharmacological Characteristics and Efficacy of Fluazinam Against Corynespora cassiicola, Causing Cucumber Target Spot in Greenhouses. Plant Disease. 2020; 104 (9):2449-2454.
Chicago/Turabian StyleTao Li; Miss Qian Xiu; Jie Zhang; Jian Xin Wang; Ya Bing Duan; Ming Guo Zhou. 2020. "Pharmacological Characteristics and Efficacy of Fluazinam Against Corynespora cassiicola, Causing Cucumber Target Spot in Greenhouses." Plant Disease 104, no. 9: 2449-2454.
Fusarium graminearum causes Fusarium head blight (FHB), a destructive disease of cereal crops worldwide. Carbendazim (methylbenzimidazol-2-ylcarbamate [MBC]) is widely used for controlling FHB. A previous study showed that the F240L mutation in the β2-tubulin of F. graminearum (Fgβ2-tubulin) confers hypersensitivity to MBC. Whether the substitution of phenylalanine by other amino acids in position 240 of the Fgβ2-tubulin gene also confers hypersensitivity to MBC is unknown. Moreover, the biological fitness of these mutants is poorly understood. In this study, we substituted position 240 of Fgβ2-tubulin with other amino acids. We found that the F240A, F240E, F240I, and F240Y mutations in Fgβ2-tubulin could also confer F. graminearum hypersensitivity to MBC, although the effective concentration resulting in 50% inhibition (EC50) differed among the mutations. The F240G mutation, in contrast, decreased the sensitivity to MBC. In addition, a molecular docking assay indicated that the binding affinity between Fgβ2-tubulin and MBC were increased by the F240A, F240E, F240I, and F240Y mutations but decreased by the F240G mutation. All mutants had normal conidial morphology, but the growth rates and pathogenicity of the F240A, F240E, F240G, F240I, and F240Y mutants were significantly decreased. Moreover, the F240A and F240G mutants produced twisted hyphae. In addition, microtubules were sparse and rarely observed in β2F240A-EGFP, β2F240E-EGFP, and β2F240G-EGFP. These results indicate that position 240 (phenylalanine) is not only vital to the function of Fgβ2-tubulin but also plays an important role in regulating the sensitivity of F. graminearum to MBC. Any mutation in this site would be detrimental to survival.
Yuanye Zhu; YuanShuai Zhang; Zongzhe He; Yabing Duan; Yanjun Li; Jian-Xin Wang; Mingguo Zhou. Detrimental Effects of Multiple Mutations in Position 240 of Fusarium graminearum β2-Tubulin. Phytopathology® 2020, 110, 1522 -1529.
AMA StyleYuanye Zhu, YuanShuai Zhang, Zongzhe He, Yabing Duan, Yanjun Li, Jian-Xin Wang, Mingguo Zhou. Detrimental Effects of Multiple Mutations in Position 240 of Fusarium graminearum β2-Tubulin. Phytopathology®. 2020; 110 (9):1522-1529.
Chicago/Turabian StyleYuanye Zhu; YuanShuai Zhang; Zongzhe He; Yabing Duan; Yanjun Li; Jian-Xin Wang; Mingguo Zhou. 2020. "Detrimental Effects of Multiple Mutations in Position 240 of Fusarium graminearum β2-Tubulin." Phytopathology® 110, no. 9: 1522-1529.
BACKGROUND Fusarium asiaticum is one of predominant pathogens of Fusarium head blight (FHB) in China. Pydiflumetofen (Pyd) is a novel succinate dehydrogenase inhibitor (SDHI) which has been commercialized in China for controlling of wheat FHB since 2019. In current study, risk assessment of the pydiflumetofen‐resistance selected in Fusarium asiaticum was investigated. RESULTS One PydMR mutant (RF3000) were generated by fungicide‐taming from 1000 mycelial discs of the wild‐type strain 2021. Nucleotide sequences alignment results of FaSdh from the wild‐type strain and resistant mutants showed that all the mutations were categorized into three genotypes, i.e., FaSdhBH248Y from PydMR mutant, both FaSdhC1A64V and FaSdhC1R67K from PydHR mutants. All the resistant mutants possessed no fitness penalty based on the data of mycelial linear growth, conidiation and virulence. In addition, the FaSdhC1A64V mutants showed positive cross‐resistance between pydiflumetofen and boscalid or thifluzamide, but no cross‐resistance between pydiflumetofen and Y13149 or Y12196, while the FaSdhC1R67K mutants exhibited positive cross‐resistance between pydiflumetofen and boscalid, thifluzamide or Y12196, and no cross‐resistance between pydiflumetofen and Y13149. Furthermore, positive cross‐resistance between the five tested SDHIs was detected in the FaSdhBH248Y mutants. CONCLUSION The above results suggest a moderate~high resistance risk of F. asiaticum to pydiflumetofen, and provide essential data for monitoring the emergence of resistance and resistance management strategies for pydiflumetofen, which will be useful for scientific application of this fungicide in China. This article is protected by copyright. All rights reserved.
Wenchan Chen; Lingling Wei; Weicheng Zhao; Bingran Wang; Huanhuan Zheng; Pengcheng Zhang; Tiancheng Lou; Yabing Duan; Yiping Hou; Mingguo Zhou; Changjun Chen. Resistance risk assessment for a novel succinate dehydrogenase inhibitor pydiflumetofen in Fusarium asiaticum. Pest Management Science 2020, 77, 538 -547.
AMA StyleWenchan Chen, Lingling Wei, Weicheng Zhao, Bingran Wang, Huanhuan Zheng, Pengcheng Zhang, Tiancheng Lou, Yabing Duan, Yiping Hou, Mingguo Zhou, Changjun Chen. Resistance risk assessment for a novel succinate dehydrogenase inhibitor pydiflumetofen in Fusarium asiaticum. Pest Management Science. 2020; 77 (1):538-547.
Chicago/Turabian StyleWenchan Chen; Lingling Wei; Weicheng Zhao; Bingran Wang; Huanhuan Zheng; Pengcheng Zhang; Tiancheng Lou; Yabing Duan; Yiping Hou; Mingguo Zhou; Changjun Chen. 2020. "Resistance risk assessment for a novel succinate dehydrogenase inhibitor pydiflumetofen in Fusarium asiaticum." Pest Management Science 77, no. 1: 538-547.
Cucumber (Cucumis sativus L.), an important vegetable with high economic value, has become one of the most economically important domestic vegetables in China. In April 2019, a previously undescribed crown rot with about 80 % incidence was observed in many vegetable greenhouses in Huaian city, Jiangsu Province. Typical symptoms of the infected fruits appeared that young fruit withered and were covered with abundant white mycelia (Figure S1). The symptoms were similar to fruit rot caused by Botrytis cinerea. It is possible that this similarity has caused this disease to be overlooked. The pathogen was isolated as followed: diseased tissues were washed by sterile water and potato dextrose agar (PDA) plates were inoculated with mycelium from diseased fruit. After incubation for 7 days at 25 °C in the dark, the spores were isolated from each colony and single spore was isolated for pure culture. After single spore isolation, fifty-six isolates with similar morphological characteristics were obtained. After incubation for 7 days at 25 °C, the colony with abundant whitish to yellowish aerial mycelia produced pale brown to dark brown pigment, and numerous falciform macroconidia (20.4 to 59 × 2.9 to 5.1 μm) in PDA plates. Moreover, macroconidia (3-5 septa) exhibited pronounced dorsiventral curvature, with tapered and elongated apical cell and prominent foot-shaped basal cell. These morphological characteristics of the isolates are consistent with that of Fusarium incarnatum described in previous studies (Gai et al. 2016; Ramdial et al. 2015). For molecular identification, the internal transcribed spacer (ITS) region of ribosomal DNA, a partial elongation factor-1 alpha (EF-1α) gene, Histone 3 gene (H3) and β-tubulin gene of sixteen isolates were amplified and sequenced using the primer pairs ITS4/ITS5 (Lu et al. 2014), EF-1/EF-2 (O’Donnell et al. 1998), H3-1a/1b and Bt2a/2b (Glass and Donaldson. 1995). The sequences of each gene for sixteen isolates were completely consistent and deposited in NCBI GenBank nucleotide database (MN227262 for the ITS region; MN233576 for β-tubulin gene; MN233577 for EF-1α gene and MN233578 for H3 gene) and showed 99.65%; 100%; 100% and 100% identity to Fusarium incarnatum species (MH979697 for ITS region, KJ020861 for β-tubulin gene, JX269001 for EF-1α gene and MH979696 for H3 gene), respectively. To confirm the pathogenicity of the isolates, 10 μL of conidial suspension (106 conidia /mL) of each isolate was inoculated on nine healthy fruits wounded with a sterilized needle, and another nine fruits were inoculated with sterilized water as controls. Six isolates and two cucumber cultivars were tested. All the inoculated fruits were placed under 25 °C and 90 % relative humidity. After about 5-7 days, they were similar to the symptoms observed on cucumber fruit in the greenhouses, but no symptoms were observed on the control fruits. The experiment was performed three times. Furthermore, F. incarnatum was re-isolated from the infected cucumber fruit fulfilling the Koch's postulates. To our knowledge, this is the first report of F. incarnatum causing crown rot on cucumber in China. Though cucumber crown rot is reported as an emerging vegetable disease, it is very serious in cucumber greenhouses, causing severe economic losses to cucumber industry in China.
Y. S. Mao; Y. Zhang; M. G. Zhou; Y. B. Duan. Occurrence of Crown Rot Disease Caused by Fusarium incarnatum on Cucumber (Cucumis sativus) in China. Plant Disease 2020, 104, 593 .
AMA StyleY. S. Mao, Y. Zhang, M. G. Zhou, Y. B. Duan. Occurrence of Crown Rot Disease Caused by Fusarium incarnatum on Cucumber (Cucumis sativus) in China. Plant Disease. 2020; 104 (2):593.
Chicago/Turabian StyleY. S. Mao; Y. Zhang; M. G. Zhou; Y. B. Duan. 2020. "Occurrence of Crown Rot Disease Caused by Fusarium incarnatum on Cucumber (Cucumis sativus) in China." Plant Disease 104, no. 2: 593.
Rice blast, caused by Magnaporthe oryzae, is a destructive fungal disease in rice, causing serious losses in yield and quality. Coumoxystrobin is a novel methoxyacrylate strobilurin fungicide. In the current study, we determined the sensitivity of 100 M. oryzae strains to coumoxystrobin based on the mycelial growth inhibition method. The EC50 values ranged from 0.0089 to 0.0290 μg mL−1, with a mean EC50 value of 0.0163 ± 0.0036 μg mL−1, indicating that coumoxystrobin exhibits an excellent inhibitory activity in the mycelial growth of M. oryzae. In addition, the EC50 values had no significant difference among four populations from the different geographical regions. After treating with coumoxystrobin, cell membrane permeability increased, respiration decreased, and the hyphal tips were contorted, with offshoot of top increasing. Protective and curative activity tests showed that coumoxystrobin exhibited better protective and curative activities against M. oryzae in detached barley leaves in comparison to the currently used fungicides tricyclazole and azoxystrobin. Also, it was found that the protective activity was better than its curative activity. Furthermore, compared with the currently used fungicides, coumoxystrobin not only exhibited excellent control efficacy on rice blast, but also markedly reduced the dosages of chemical fungicides in the field trials. Overall, these findings provide important references for revealing the pharmacological effect of coumoxystrobin against M. oryzae and managing rice blast caused by M. oryzae.
Wenjing Xin; Yushuai Mao; Fei Lu; Tao Li; Jianxin Wang; Yabing Duan; Mingguo Zhou. In vitro fungicidal activity and in planta control efficacy of coumoxystrobin against Magnaporthe oryzae. Pesticide Biochemistry and Physiology 2019, 162, 78 -85.
AMA StyleWenjing Xin, Yushuai Mao, Fei Lu, Tao Li, Jianxin Wang, Yabing Duan, Mingguo Zhou. In vitro fungicidal activity and in planta control efficacy of coumoxystrobin against Magnaporthe oryzae. Pesticide Biochemistry and Physiology. 2019; 162 ():78-85.
Chicago/Turabian StyleWenjing Xin; Yushuai Mao; Fei Lu; Tao Li; Jianxin Wang; Yabing Duan; Mingguo Zhou. 2019. "In vitro fungicidal activity and in planta control efficacy of coumoxystrobin against Magnaporthe oryzae." Pesticide Biochemistry and Physiology 162, no. : 78-85.
Microtubule is a well-known structural protein participating in cell division, motility and vesicle traffic. In this study, we found that β2-tubulin, one of the microtubule components, plays an important role in regulating secondary metabolite deoxynivalenol (DON) biosynthesis in Fusarium graminearum by interacting with isocitrate dehydrogenase subunit 3 (IDH3). We found IDH3 negatively regulate DON biosynthesis by reducing acetyl-CoA accumulation in F. graminearum and DON biosynthesis was stimulated by exogenous acetyl-CoA. In addition, the expression of IDH3 significantly decreased in the carbendazim-resistant mutant nt167 (Fgβ F167Y). Furthermore, we found that carbendazim-resistance associated β2-tubulin substitutions reducing the interaction intensity between β2-tubulin and IDH3. Interestingly, we demonstrated that β2-tubulin inhibitor carbendazim can disrupt the interaction between β2-tubulin and IDH3. The decreased interaction intensity between β2-tubulin and IDH3 resulted in the decreased expression of IDH3, which can cause the accumulation of acetyl-CoA, precursor of DON biosynthesis in F. graminearum. Thus, we revealed that carbendazim-resistance associated β2-tubulin substitutions or carbendazim treatment increases DON biosynthesis by reducing the interaction between β2-tubulin and IDH3 in F. graminearum. Taken together, the novel findings give the new perspectives of β2-tubulin in regulating secondary metabolism in phytopathogenic fungi.Author SummaryThe deoxynivalenol (DON) biosynthesis is increased in carbendazim-resistant strains in Fusarium graminearum. To date, the molecular mechanism between the carbendazim-resistant substitution and the increased DON production remained elusive. Here we found that acetyl-CoA-associated enzyme IDH3 negatively regulates acetyl-CoA and DON biosynthesis. Moreover, β2 tubulin interacted with IDH3 physically and increase its expression. We further found that carbendazim-resistant substitution in β2 tubulin reducing the interaction between β2 tubulin and IDH3, which resulted in the decreased expression of IDH3. In addition, we demonstrated that carbendazim disrupting the binding between β2 tubulin and IDH3, which also decreases the expression of IDH3. Taken together, our results give a newly insights into the mechanism of β2 tubulin and its carbendazim-resistant substitution in regulating DON biosynthesis.
Zehua Zhou; Yabing Duan; Mingguo Zhou. Carbendazim-resistance associated β2-tubulin substitutions increase deoxynivalenol biosynthesis by reducing the interaction between β2-tubulin and IDH3 in Fusarium graminearum. 2019, 760595 .
AMA StyleZehua Zhou, Yabing Duan, Mingguo Zhou. Carbendazim-resistance associated β2-tubulin substitutions increase deoxynivalenol biosynthesis by reducing the interaction between β2-tubulin and IDH3 in Fusarium graminearum. . 2019; ():760595.
Chicago/Turabian StyleZehua Zhou; Yabing Duan; Mingguo Zhou. 2019. "Carbendazim-resistance associated β2-tubulin substitutions increase deoxynivalenol biosynthesis by reducing the interaction between β2-tubulin and IDH3 in Fusarium graminearum." , no. : 760595.
Deoxynivalenol (DON) is a class of mycotoxin produced in cereal crops infected with Fusarium graminearum species complex (FGSC). In China, FGSC mainly includes Fusarium asiaticum and F. graminearum. DON belongs to the trichothecenes and poses a serious threat to the safety and health of humans and animals. Succinate dehydrogenase inhibitors (SDHIs) are a class of fungicides that act on succinate dehydrogenase and inhibit the respiration of pathogenic fungi. In this study, the fungicidal activities of five SDHIs, including fluopyram, flutolanil, boscalid, benzovindiflupyr, and fluxapyroxad, against FGSC were determined based on mycelial growth and spore germination inhibition methods. The five SDHIs exhibited better inhibitory activities in spore germination than mycelial growth. Fluopyram exhibited a higher inhibitory effect in mycelial growth and spore germination in comparison to the other four SDHIs. In addition, the biological characteristics of F. asiaticum as affected by the five SDHIs were determined. We found that these five SDHIs decreased DON, pyruvic acid and acetyl-CoA production, isocitrate dehydrogenase mitochondrial (ICDHm) and SDH activities, and NADH and ATP content of F. asiaticum but increased the citric acid content. In addition, TRI5 gene expression was inhibited, and the formation of toxisomes was disrupted by the five SDHIs, further confirming that SDHIs can decrease DON biosynthesis of F. asiaticum. Thus, we concluded that SDHIs may decrease DON biosynthesis of F. asiaticum by inhibiting glycolysis and the tricarboxylic acid (TCA) cycle. Overall, the findings from the study will provide important references for managing Fusarium head blight (FHB) caused by FGSC and reducing DON contamination in F. asiaticum-infected wheat grains.
Chao Xu; Meixia Li; Zehua Zhou; Jiaosheng Li; Dongming Chen; Yabing Duan; Mingguo Zhou. Impact of Five Succinate Dehydrogenase Inhibitors on DON Biosynthesis of Fusarium asiaticum, Causing Fusarium Head Blight in Wheat. Toxins 2019, 11, 272 .
AMA StyleChao Xu, Meixia Li, Zehua Zhou, Jiaosheng Li, Dongming Chen, Yabing Duan, Mingguo Zhou. Impact of Five Succinate Dehydrogenase Inhibitors on DON Biosynthesis of Fusarium asiaticum, Causing Fusarium Head Blight in Wheat. Toxins. 2019; 11 (5):272.
Chicago/Turabian StyleChao Xu; Meixia Li; Zehua Zhou; Jiaosheng Li; Dongming Chen; Yabing Duan; Mingguo Zhou. 2019. "Impact of Five Succinate Dehydrogenase Inhibitors on DON Biosynthesis of Fusarium asiaticum, Causing Fusarium Head Blight in Wheat." Toxins 11, no. 5: 272.
Fusarium graminearum species complex (FGSC), causing Fusarium head blight (FHB) of wheat, has species-specific geographical distributions in wheat-growing regions. In recent years, benzimidazole resistance of FHB pathogens has been largely widespread in China. Although the demethylation inhibitor fungicide metconazole has been used for FHB control in some countries, no information about metconazole sensitivity of Chinese FHB pathogen populations and efficacy of metconazole in FHB control in China is available. In this study, the sensitivity of FGSC to metconazole was measured with 32 carbendazim-sensitive strains and 35 carbendazim-resistant strains based on mycelial growth. The 50% effective concentration values of 67 strains were normally distributed and ranged from 0.0209 to 0.0838 μg ml−1, with a mean of 0.0481 ± 0.0134 μg ml−1. No significant difference in metconazole sensitivity was observed between carbendazim-sensitive and -resistant populations. An interactive effect of metconazole and phenamacril, a novel cyanoacrilate fungicide approved in China against Fusarium spp., in inhibiting mycelial growth showed an additive interaction at different ratios. Furthermore, field trials to evaluate the effect of metconazole and metconazole + phenamacril treatments in FHB control, deoxynivalenol (DON) production, and grain yields were performed. Compared with the fungicides carbendazim and phenamacril currently used in China, metconazole exhibits a better efficacy for FHB control, DON production, and grain yields, and dramatically reduces use dosages of chemical compounds in the field. The mixture of metconazole and phenamacril at ratios of 2:3 and 1:2 showed the greatest efficacy for FHB control, DON production, and grain yields among all the fungicide treatments but its use dosages were higher in comparison with metconazole alone. In addition, FHB control, grain yields, and DON levels were significantly correlated with each other, showing that visual disease indices can be used as an indicator of grain yields and DON contamination. Meanwhile, the frequency of carbendazim-resistant alleles in F. graminearum populations was dramatically reduced after metconazole and phenamacril alone and the mixture of metconazole and phenamacril applications, indicating that metconazole and a mixture of metconazole and phenamacril can be used for carbendazim resistance management of FHB in wheat. Overall, the findings of this study provide important data for resistance management of FHB and reducing DON contamination in wheat grains.
Yabing Duan; Xian Tao; Huahua Zhao; Xuemei Xiao; Meixia Li; Jianxin Wang; Mingguo Zhou. Activity of Demethylation Inhibitor Fungicide Metconazole on Chinese Fusarium graminearum Species Complex and Its Application in Carbendazim-Resistance Management of Fusarium Head Blight in Wheat. Plant Disease 2019, 103, 929 -937.
AMA StyleYabing Duan, Xian Tao, Huahua Zhao, Xuemei Xiao, Meixia Li, Jianxin Wang, Mingguo Zhou. Activity of Demethylation Inhibitor Fungicide Metconazole on Chinese Fusarium graminearum Species Complex and Its Application in Carbendazim-Resistance Management of Fusarium Head Blight in Wheat. Plant Disease. 2019; 103 (5):929-937.
Chicago/Turabian StyleYabing Duan; Xian Tao; Huahua Zhao; Xuemei Xiao; Meixia Li; Jianxin Wang; Mingguo Zhou. 2019. "Activity of Demethylation Inhibitor Fungicide Metconazole on Chinese Fusarium graminearum Species Complex and Its Application in Carbendazim-Resistance Management of Fusarium Head Blight in Wheat." Plant Disease 103, no. 5: 929-937.
Phenazine-1-carboxylic acid (PCA), a secondary metabolite produced by Pseudomonas spp., exhibits a high inhibitory effect in Xanthomonas oryzae pv. oryzae (Xoo), but less inhibitory effect in Xanthomonas oryzae pv. oryzicola (Xoc), and almost no inhibitory effect in Xanthomonas campestris pv. campestris (Xcc). In our previous study, reactive oxygen species (ROS) scavenging system was reported to be involved in PCA tolerance in Xanthomonas spp. However, the PCA tolerance mechanism of Xanthomonas spp. is unclear. In the current study, we constructed a Tn5-based transposon mutant library in Xcc and four highly PCA-sensitive insertion mutants were obtained. TAIL-PCR further confirmed that the Tn5 transposon was inserted in the cytochrome c maturation (CCM) system (XC_1893, XC_1897) of these mutants. Disruption of the CCM system significantly decreased the growth, motility and tolerance of Xcc to PCA and other phenazines, such as phenazine and 1-OH-phenazine. The CCM system is responsible for the covalent attachment of the apocytochrome and heme. Disruption of the transmembrane thioredox protein (Dsb) pathway (XC_0531), an essential process for the formation of mature apocytochrome, also exhibited a decreased tolerance to PCA, suggesting that the defect of cytochrome c caused decreased tolerance of Xcc to PCA. Meanwhile, disruption of the CCM system or Dsb pathway interfered with the functions of cytochrome c proteins, causing an increased sensitivity to H2O2. Collectively, we concluded that the CCM system and Dsb pathway, regulate the tolerance of Xcc to phenazines by influencing the functions of cytochrome c. Therefore, these results provide important references for revealing the action mechanism of PCA in Xanthomonas spp.
Jian Wu; Xiayan Pan; Shu Xu; Yabing Duan; Jianying Luo; Zehua Zhou; Jianxin Wang; Mingguo Zhou. The critical role of cytochrome c maturation (CCM) system in the tolerance of Xanthomonas campestris pv. campestris to phenazines. Pesticide Biochemistry and Physiology 2019, 156, 63 -71.
AMA StyleJian Wu, Xiayan Pan, Shu Xu, Yabing Duan, Jianying Luo, Zehua Zhou, Jianxin Wang, Mingguo Zhou. The critical role of cytochrome c maturation (CCM) system in the tolerance of Xanthomonas campestris pv. campestris to phenazines. Pesticide Biochemistry and Physiology. 2019; 156 ():63-71.
Chicago/Turabian StyleJian Wu; Xiayan Pan; Shu Xu; Yabing Duan; Jianying Luo; Zehua Zhou; Jianxin Wang; Mingguo Zhou. 2019. "The critical role of cytochrome c maturation (CCM) system in the tolerance of Xanthomonas campestris pv. campestris to phenazines." Pesticide Biochemistry and Physiology 156, no. : 63-71.
Pydiflumetofen is a novel succinate dehydrogenase inhibitor fungicide. In the current research, we determined the sensitivity of 166 Sclerotinia sclerotiorum strains to pydiflumetofen using mycelial growth inhibition method. The results suggest that pydiflumetofen exhibited a strong inhibitory activity against S. sclerotiorum and the EC50 values ranged from 0.0058 to 0.0953 μg ml−1, with a mean EC50 value of 0.0250 μg ml−1. However, the baseline sensitivity was not normally distributed because of a high variation factor. After treatment with pydiflumetofen, cell membrane permeability increases, but exopolysaccharide and oxalic acid production decreases, which contributes to reduced virulence of S. sclerotiorum and leads to failure of disease infection. In addition, protective and curative activity was performed on detached oilseed rape leaves by artificial inoculation. Pydiflumetofen exhibited excellent protective and curative effects against S. sclerotiorum on oilseed rape, and the protective effect was better than the curative effect. Further, field trials were conducted to evaluate the potential of pydiflumetofen in controlling Sclerotinia stem rot (SSR) caused by S. sclerotiorum on oilseed rape. Compared with the currently used fungicides, pydiflumetofen not only exhibited excellent control efficacy against SSR, but also dramatically reduced the dosage of fungicides in the field. Thus, this study provides important references for revealing pharmacological mechanism of pydiflumetofen against S. sclerotiorum and managing SSR on oilseed rape caused by benzimidazole- and dicarboximide-resistant populations.
Yabing Duan; Qian Xiu; Haoran Li; Tao Li; Jianxin Wang; Mingguo Zhou. Pharmacological Characteristics and Control Efficacy of a Novel SDHI Fungicide Pydiflumetofen Against Sclerotinia sclerotiorum. Plant Disease 2019, 103, 77 -82.
AMA StyleYabing Duan, Qian Xiu, Haoran Li, Tao Li, Jianxin Wang, Mingguo Zhou. Pharmacological Characteristics and Control Efficacy of a Novel SDHI Fungicide Pydiflumetofen Against Sclerotinia sclerotiorum. Plant Disease. 2019; 103 (1):77-82.
Chicago/Turabian StyleYabing Duan; Qian Xiu; Haoran Li; Tao Li; Jianxin Wang; Mingguo Zhou. 2019. "Pharmacological Characteristics and Control Efficacy of a Novel SDHI Fungicide Pydiflumetofen Against Sclerotinia sclerotiorum." Plant Disease 103, no. 1: 77-82.
Ying Yang; Mei-Xia Li; Ya-Bing Duan; Tao Li; Yi-Yuan Shi; Dong-Lei Zhao; Ze-Hua Zhou; Wen-Jing Xin; Jian Wu; Xia-Yan Pan; Yan-Jun Li; Yuan-Ye Zhu; Ming-Guo Zhou. Corrigendum to "A new point mutation in β2-tubulin confers resistance to carbendazim in Fusarium asiaticum" [Pestic. Biochem. Physiol. 145 (2018) 15-21]. Pesticide Biochemistry and Physiology 2018, 154, 97 .
AMA StyleYing Yang, Mei-Xia Li, Ya-Bing Duan, Tao Li, Yi-Yuan Shi, Dong-Lei Zhao, Ze-Hua Zhou, Wen-Jing Xin, Jian Wu, Xia-Yan Pan, Yan-Jun Li, Yuan-Ye Zhu, Ming-Guo Zhou. Corrigendum to "A new point mutation in β2-tubulin confers resistance to carbendazim in Fusarium asiaticum" [Pestic. Biochem. Physiol. 145 (2018) 15-21]. Pesticide Biochemistry and Physiology. 2018; 154 ():97.
Chicago/Turabian StyleYing Yang; Mei-Xia Li; Ya-Bing Duan; Tao Li; Yi-Yuan Shi; Dong-Lei Zhao; Ze-Hua Zhou; Wen-Jing Xin; Jian Wu; Xia-Yan Pan; Yan-Jun Li; Yuan-Ye Zhu; Ming-Guo Zhou. 2018. "Corrigendum to "A new point mutation in β2-tubulin confers resistance to carbendazim in Fusarium asiaticum" [Pestic. Biochem. Physiol. 145 (2018) 15-21]." Pesticide Biochemistry and Physiology 154, no. : 97.
Validamycin, known to interfere with fungal energy metabolism by inhibiting trehalase, has been extensively used to control plant diseases caused by Rhizoctonia spp. However, the effect of validamycin on controlling Fusarium graminearum has not been previously reported. In this study, when applied to F. graminearum in vitro, validamycin inhibited the synthesis of deoxynivalenol (DON), which is a mycotoxin and virulence factor, by decreasing trehalase activity and the production of glucose and pyruvate, which are precursors of DON biosynthesis. Because FgNTH encodes the main trehalase in F. graminearum, these effects were nullified in the FgNTH deletion mutant ΔFgNTH but restored in the complemented strain ΔFgNTHC. In addition, validamycin also increased the expression of pathogenesis-related genes (PRs) PR1, PR2, and PR5 in wheat, inducing resistance responses of wheat against F. graminearum. Therefore, validamycin exhibits dual efficacies on controlling Fusarium head blight (FHB) caused by F. graminearum: inhibition of DON biosynthesis and induction of host resistance. In addition, field trials further confirmed that validamycin increased FHB control and reduced DON contamination in grain. Control of FHB and DON contamination by validamycin increased when the antibiotic was applied with the triazole fungicide metconazole. Overall, this study is a successful case from foundational research to applied research, providing useful information for wheat protection programs against toxigenic fungi responsible for FHB and the consequent mycotoxin accumulation in grains.
Jing Li; Yabing Duan; Chuanhong Bian; Xiayan Pan; Chengjie Yao; Jianxin Wang; Mingguo Zhou. Effects of validamycin in controlling Fusarium head blight caused by Fusarium graminearum: Inhibition of DON biosynthesis and induction of host resistance. Pesticide Biochemistry and Physiology 2018, 153, 152 -160.
AMA StyleJing Li, Yabing Duan, Chuanhong Bian, Xiayan Pan, Chengjie Yao, Jianxin Wang, Mingguo Zhou. Effects of validamycin in controlling Fusarium head blight caused by Fusarium graminearum: Inhibition of DON biosynthesis and induction of host resistance. Pesticide Biochemistry and Physiology. 2018; 153 ():152-160.
Chicago/Turabian StyleJing Li; Yabing Duan; Chuanhong Bian; Xiayan Pan; Chengjie Yao; Jianxin Wang; Mingguo Zhou. 2018. "Effects of validamycin in controlling Fusarium head blight caused by Fusarium graminearum: Inhibition of DON biosynthesis and induction of host resistance." Pesticide Biochemistry and Physiology 153, no. : 152-160.