Melatonin is a ubiquitous molecule distributed in nature and not only plays an important role in animals and humans but also has extensive functions in plants, such as delaying senescence, exerting antioxidant effects, regulating growth and development, and facilitating plant adaption to stress conditions. Endogenous melatonin is widespread in fruits and vegetables and plays prominent roles in the ripening and post-harvest process of fruits and vegetables. Exogenous application of melatonin removes excess reactive oxygen species from post-harvest fruits and vegetables by increasing antioxidant enzymes, non-enzymatic antioxidants, and enzymes related to oxidized protein repair. Moreover, exogenous application of melatonin can increase endogenous melatonin to augment its effects on various physiological processes. Many previous reports have demonstrated that application of exogenous melatonin improves the post-harvest preservation of fruits and vegetables. Although overproduction of melatonin in plants via transgenic approaches could be a potential means for improving the post-harvest preservation of fruits and vegetables, efforts to increase endogenous melatonin in plants are limited. In this review, we summarize the recent progress revealing the role and action mechanisms of melatonin in post-harvest fruits and vegetables and provide future directions for the utilization of melatonin to improve the post-harvest preservation of fruits and vegetables.

Sweet potato (Ipomoea batatas L.) is the sixth most important food crop in the world. The formation and development of storage roots in sweet potato is a highly complicated and genetically programmed process. However, the underlying mechanisms of storage root development have not yet been elucidated. To better understand the molecular mechanisms involved in storage root development, a combined analysis of the transcriptome and proteome of sweet potato fibrous roots (F) and storage roots at four different stages (D1, D3, D5 and D10) was performed in the present study. A total of 26,273 differentially expressed genes were identified in a comparison between the fibrous root library and four storage root libraries, while 2558 proteins showed a 1.0-fold or greater expression difference as indicated by isobaric tags for relative and absolute quantitation (iTRAQ) analysis. The combination of the transcriptome and proteome analyses and morphological and physiological data revealed several critical pathways involved in storage root formation and development. First, genes/proteins involved in the development of meristems/cambia and starch biosynthesis were all significantly upregulated in storage roots compared with fibrous roots. Second, multiple phytohormones and the genes related to their biosynthesis showed differential expression between fibrous roots and storage roots. Third, a large number of transcription factors were differentially expressed during storage root initiation and development, which suggests the importance of transcription factor regulation in the development of storage roots. Fourth, inconsistent gene expression was found between the transcriptome and proteome data, which indicated posttranscriptional regulatory activity during the development of storage roots. Overall, these results reveal multiple events associated with storage root development and provide new insights into the molecular mechanisms underlying the regulatory networks involved in storage root development.

N6-methyladenosine (m⁶A) is the most abundant internal modification in eukaryotic messenger RNA. Although the role of m⁶A has been demonstrated in many biological processes, including embryonic development, flowering time control, microspore generation, fruit ripening, and stress responses, its contribution to other aspects of plant development still needs to be explored. Herein, we show the potential link between m⁶A deposition and the expansion of tomato (Solanum lycopersicum) fruits through parallel m⁶A-immunoprecipitation-sequencing (m⁶A-seq) and RNA-seq analyses. We found that global m⁶A levels increased during tomato fruit expansion from immature green to mature green stage. m⁶A-seq revealed that thousands of protein-coding genes are m⁶A-modified mainly in the 3ʹ-untranslated regions. m⁶A-seq and RNA-seq analyses showed a positive association between m⁶A methylation and mRNA abundance. In particular, a large number of fruit expansion-related genes involved in hormone responses and endoreduplication were m⁶A modified and expressed more actively than the non-m⁶A-modified genes, suggesting a potential role of m⁶A modification in tomato fruit expansion. Importantly, altering m⁶A levels by direct injection of 3-deazaneplanocin A (DA; m⁶A writer inhibitor) or meclofenamic acid (MA; m⁶A eraser inhibitor) into tomato fruits suppressed fruit expansion; however, injection of exogenous DA or MA accelerated or delayed fruit ripening, respectively. Collectively, these results suggest a dynamic role of m⁶A methylation in the expansion and ripening of tomato fruits.

Epitranscriptomic chemical modifications of RNAs have emerged as potent regulatory mechanisms in the process of plant stress adaptation. Currently, over 170 distinct chemical modifications have been identified in mRNAs, tRNAs, rRNAs, microRNAs (miRNAs), and long noncoding RNAs (lncRNAs). Genetic and molecular studies have identified the genes responsible for addition and removal of chemical modifications from RNA molecules, which are known as "writers" and "erasers," respectively. N⁶-methyladenosine (m⁶A) is the most prevalent chemical modification identified in eukaryotic mRNAs. Recent studies have identified m⁶A writers and erasers across different plant species, including Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), cotton (Gossypium hirsutum), and tomato (Solanum lycopersicum). Accumulating discoveries have improved our understanding of the functions of RNA modifications in plant stress responses. This review highlights the latest research on RNA modification, emphasizing the biological and cellular roles of diverse chemical modifications of mRNAs, tRNAs, rRNAs, miRNAs, and lncRNAs in plant responses to environmental and hormonal signals. We also propose and discuss critical questions and future challenges for enhancing our understanding of the cellular and mechanistic roles of RNA modifications in plant stress responses. Integrating molecular insights into the regulatory roles of RNA modifications in stress responses with novel genome- and RNA-editing technologies will facilitate the breeding of stress-tolerant crops through precise engineering of RNA modifications.

Background: MicroRNAs (miRNAs), a class of small regulatory RNAs, have been proven to play important roles in plant growth, development and stress responses. Sweet potato (Ipomoea batatasL.) is an important food and industrial crop that ranks seventh in staple food production. However, the regulatory mechanism of miRNA-mediated abiotic stress response in sweet potato remains unclear.Results: In this study, we employed deep sequencing to identify both conserved and novel miRNAs from salinity-exposed sweet potato cultivars and its untreated control. Twelve small non-coding RNA libraries from NaCl-free (CK) and NaCl-treated (Na150) sweet potato leaves and roots were constructed for salt-responsive miRNA identification in sweet potatoes. A total of 475 known miRNAs (belonging to 66 miRNA families) and 175 novel miRNAs were identified. Among them, 51 (22 known miRNAs and 29 novel miRNAs) were significantly up-regulated and 76 (61 known miRNAs and 15 novel miRNAs) were significantly down-regulated by salinity stress in sweet potato leaves; 13 (12 known miRNAs and 1 novel miRNAs) were significantly up-regulated and 9 (7 known miRNAs and 2 novel miRNAs) were significantly down-regulated in sweet potato roots. Furthermore, 636 target genes of 314 miRNAs were validated by degradome sequencing. Deep sequencing results confirmed by qRT-PCR experiments indicated that the expression of most miRNAs exhibit a negative correlation with the expression of their targets under salt stress.Conclusions: This study provides insights into the regulatory mechanism of miRNA-mediated salt response and molecular breeding of sweet potatoes though miRNA manipulation.