Phenotyping provides important support for corn breeding. Unfortunately, the rapid detection of phenotypes has been the major limiting factor in estimating and predicting the outcomes of breeding programs. This study was focused on the potential of phenotyping to support corn breeding using unmanned aerial vehicle (UAV) images, aiming at mining and deepening UAV techniques for comparing phenotypes and screening new corn varieties. Two geometric traits (plant height, canopy leaf area index (LAI)) and one lodging resistance trait (lodging area) were estimated in this study. It was found that stereoscopic and photogrammetric methods were promising ways to calculate a digital surface model (DSM) for estimating corn plant height from UAV images, with R2 = 0.7833 (p < 0.001) and a root mean square error (RMSE) = 0.1677. In addition to a height estimation, the height variation was analyzed for depicting and validating the corn canopy uniformity stability for different varieties. For the lodging area estimation, the normalized DSM (nDSM) method was more promising than the gray-level co-occurrence matrix (GLCM) textural features method. The estimation error using the nDSM ranged from 0.8% to 5.3%, and the estimation error using the GLCM ranged from 10.0% to 16.2%. Associations between the height estimation and lodging area estimation were done to find the corn varieties with optimal plant heights and lodging resistance. For the LAI estimation, the physical radiative transfer PROSAIL model offered both an accurate and robust estimation performance both at the middle (R2 = 0.7490, RMSE = 0.3443) and later growing stages (R2 = 0.7450, RMSE = 0.3154). What was more exciting was that the estimated sequential time series LAIs revealed a corn variety with poor resistance to lodging in a study area of Baogaofeng Farm. Overall, UAVs appear to provide a promising method to support phenotyping for crop breeding, and the phenotyping of corn breeding in this study validated this application.
Leaf angle is a critical structural parameter for retrieving canopy leaf area index (LAI) using the PROSAIL model. However, the traditional method using default leaf angle distribution in the PROSAIL model does not capture the phenological dynamics of canopy growth. This study presents a LAI retrieval method for corn canopies using PROSAIL model with leaf angle distribution functions referred from terrestrial laser scanning points at four phenological stages during the growing season. Specifically, four inferred maximum-probability leaf angles were used in the Campbell ellipsoid leaf angle distribution function of PROSAIL. A Lookup table (LUT) is generated by running the PROSAIL model with inferred leaf angles, and the cost function is minimized to retrieve LAI. The results show that the leaf angle distribution functions are different for the corn plants at different phenological growing stages, and the incorporation of derived specific corn leaf angle distribution functions distribute the improvement of LAI retrieval using the PROSAIL model. This validation is done using in-situ LAI measurements and MODIS LAI in Baoding City, Hebei Province, China, and compared with the LAI retrieved using default leaf angle distribution function at the same time. The root-mean-square error (RMSE) between the retrieved LAI on 4 September 2014, using the modified PROSAIL model and the in-situ measured LAI was 0.31 m2/m2, with a strong and significant correlation (R2 = 0.82, residual range = 0 to 0.6 m2/m2, p < 0.001). Comparatively, the accuracy of LAI retrieved results using default leaf angle distribution is lower, the RMSE of which is 0.56 with R2 = 0.76 and residual range = 0 to 1.0 m2/m2, p < 0.001. This validation reveals that the introduction of inferred leaf angle distributions from TLS data points can improve the LAI retrieval accuracy using the PROSAIL model. Moreover, the comparations of LAI retrieval results on 10 July, 26 July, 19 August and 4 September with default and inferred corn leaf angle distribution functions are all compared with MODIS LAI products in the whole study area. This validation reveals that improvement exists in a wide spatial range and temporal range. All the comparisons demonstrate the potential of the modified PROSAIL model for retrieving corn canopy LAI from Landsat imagery by inferring leaf orientation from terrestrial laser scanning data.
Continuous monitoring of crop growth status using time-series remote sensing image is essential for crop management and yield prediction. The growing season of summer corn in the North China Plain with the period of rain and hot, which makes the acquisition of cloud-free satellite imagery very difficult. Therefore, we focused on developing image datasets with both a high temporal resolution and medium spatial resolution by harmonizing the time-series of MOD09GA Normalized Difference Vegetation Index (NDVI) images and 30-m-resolution GF-1 WFV images using the improved Kalman filter model. The harmonized images, GF-1 images, and Landsat 8 images were then combined and used to monitor the summer corn growth from 5th June to 6th October, 2014, in three counties of Hebei Province, China, in conjunction with meteorological data and MODIS Evapotranspiration Data Set. The prediction residuals ( ) in NDVI between the GF-1 observations and the harmonized images was in the range of −0.2 to 0.2 with Gauss distribution. Moreover, the obtained phenological curves manifested distinctive growth features for summer corn at field scales. Changes in NDVI over time were more effectively evaluated and represented corn growth trends, when considered in conjunction with meteorological data and MODIS Evapotranspiration Data Set. We observed that the NDVI of summer corn showed a process of first decreasing and then rising in the early growing stage and discuss how the temperature and moisture of the environment changed with the growth stage. The study demonstrated that the synthesized dataset constructed using this methodology was highly accurate, with high temporal resolution and medium spatial resolution and it was possible to harmonize multi-source remote sensing imagery by the improved Kalman filter for long-term field monitoring.