This page has only limited features, please log in for full access.
Wheat is a major staple food crop worldwide, due to its total yield and unique processing quality. Its grain yield and quality are threatened by Fusarium head blight (FHB), which is mainly caused by Fusarium graminearum. Salicylic acid (SA) has a strong and toxic effect on F. graminearum and is a hopeful target for sustainable control of FHB. F. graminearum is capable of efficientdealing with SA stress. However, the underlying mechanisms remain unclear. Here, we characterized FgMFS1 (FGSG_03725), a major facilitator superfamily (MFS) transporter gene in F. graminearum. FgMFS1 was highly expressed during infection and was upregulated by SA. The predicted three-dimensional structure of the FgMFS1 protein was consistent with the schematic for the antiporter. The subcellular localization experiment indicated that FgMFS1 was usually expressed in the vacuole of hyphae, but was alternatively distributed in the cell membrane under SA treatment, indicating an element of F. graminearum in response to SA. ΔFgMFS1 (loss of function mutant of FgMFS1) showed enhanced sensitivity to SA, less pathogenicity towards wheat, and reduced DON production under SA stress. Re-introduction of a functional FgMFS1 gene into ∆FgMFS1 recovered the mutant phenotypes. Wheat spikes inoculated with ΔFgMFS1 accumulated more SA when compared to those inoculated with the wild-type strain. Ecotopic expression of FgMFS1 in yeast enhanced its tolerance to SA as expected, further demonstrating that FgMFS1 functions as an SA exporter. In conclusion, FgMFS1 encodes an SA exporter in F. graminearum, which is critical for its response to wheat endogenous SA and pathogenicity towards wheat.
Qing Chen; Lu Lei; Caihong Liu; Yazhou Zhang; Qiang Xu; Jing Zhu; Zhenru Guo; Yan Wang; Qingcheng Li; Yang Li; Li Kong; Yunfeng Jiang; Xiujin Lan; Jirui Wang; Qiantao Jiang; Guoyue Chen; Jian Ma; Yuming Wei; Youliang Zheng; Pengfei Qi. Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat. International Journal of Molecular Sciences 2021, 22, 8497 .
AMA StyleQing Chen, Lu Lei, Caihong Liu, Yazhou Zhang, Qiang Xu, Jing Zhu, Zhenru Guo, Yan Wang, Qingcheng Li, Yang Li, Li Kong, Yunfeng Jiang, Xiujin Lan, Jirui Wang, Qiantao Jiang, Guoyue Chen, Jian Ma, Yuming Wei, Youliang Zheng, Pengfei Qi. Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat. International Journal of Molecular Sciences. 2021; 22 (16):8497.
Chicago/Turabian StyleQing Chen; Lu Lei; Caihong Liu; Yazhou Zhang; Qiang Xu; Jing Zhu; Zhenru Guo; Yan Wang; Qingcheng Li; Yang Li; Li Kong; Yunfeng Jiang; Xiujin Lan; Jirui Wang; Qiantao Jiang; Guoyue Chen; Jian Ma; Yuming Wei; Youliang Zheng; Pengfei Qi. 2021. "Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat." International Journal of Molecular Sciences 22, no. 16: 8497.
Kernel size (KS) and kernel weight play a key role in wheat yield. Phenotypic data from six environments and a Wheat55K single-nucleotide polymorphism array–based constructed genetic linkage map from a recombinant inbred line population derived from the cross between the wheat line 20828 and the line SY95-71 were used to identify quantitative trait locus (QTL) for kernel length (KL), kernel width (KW), kernel thickness (KT), thousand-kernel weight (TKW), kernel length–width ratio (LWR), KS, and factor form density (FFD). The results showed that 65 QTLs associated with kernel traits were detected, of which the major QTLs QKL.sicau-2SY-1B, QKW.sicau-2SY-6D, QKT.sicau-2SY-2D, and QTKW.sicau-2SY-2D, QLWR.sicau-2SY-6D, QKS.sicau-2SY-1B/2D/6D, and QFFD.sicau-2SY-2D controlling KL, KW, KT, TKW, LWR, KS, and FFD, and identified in multiple environments, respectively. They were located on chromosomes 1BL, 2DL, and 6DS and formed three QTL clusters. Comparison of genetic and physical interval suggested that only QKL.sicau-2SY-1B located on chromosome 1BL was likely a novel QTL. A Kompetitive Allele Specific Polymerase chain reaction (KASP) marker, KASP-AX-109379070, closely linked to this novel QTL was developed and used to successfully confirm its effect in two different genetic populations and three variety panels consisting of 272 Chinese wheat landraces, 300 Chinese wheat cultivars most from the Yellow and Huai River Valley wheat region, and 165 Sichuan wheat cultivars. The relationships between kernel traits and other agronomic traits were detected and discussed. A few predicted genes involved in regulation of kernel growth and development were identified in the intervals of these identified major QTL. Taken together, these stable and major QTLs provide valuable information for understanding the genetic composition of kernel yield and provide the basis for molecular marker–assisted breeding.
Xiangru Qu; Jiajun Liu; Xinlin Xie; Qiang Xu; Huaping Tang; Yang Mu; Zhien Pu; Yang Li; Jun Ma; Yutian Gao; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Jirui Wang; Pengfei Qi; Ahsan Habib; Yuming Wei; Youliang Zheng; Xiujin Lan; Jian Ma. Genetic Mapping and Validation of Loci for Kernel-Related Traits in Wheat (Triticum aestivum L.). Frontiers in Plant Science 2021, 12, 1 .
AMA StyleXiangru Qu, Jiajun Liu, Xinlin Xie, Qiang Xu, Huaping Tang, Yang Mu, Zhien Pu, Yang Li, Jun Ma, Yutian Gao, Qiantao Jiang, Yaxi Liu, Guoyue Chen, Jirui Wang, Pengfei Qi, Ahsan Habib, Yuming Wei, Youliang Zheng, Xiujin Lan, Jian Ma. Genetic Mapping and Validation of Loci for Kernel-Related Traits in Wheat (Triticum aestivum L.). Frontiers in Plant Science. 2021; 12 ():1.
Chicago/Turabian StyleXiangru Qu; Jiajun Liu; Xinlin Xie; Qiang Xu; Huaping Tang; Yang Mu; Zhien Pu; Yang Li; Jun Ma; Yutian Gao; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Jirui Wang; Pengfei Qi; Ahsan Habib; Yuming Wei; Youliang Zheng; Xiujin Lan; Jian Ma. 2021. "Genetic Mapping and Validation of Loci for Kernel-Related Traits in Wheat (Triticum aestivum L.)." Frontiers in Plant Science 12, no. : 1.
The basic leucine zipper (bZIP) family of genes encode transcription factors that play key roles in plant growth and development. In this study, a total of 92 HvbZIP genes were identified and compared with previous studies using recently released barley genome data. Two novel genes were characterized in this study, and some misannotated and duplicated genes from previous studies have been corrected. Phylogenetic analysis results showed that 92 HvbZIP genes were classified into 10 groups and three unknown groups. The gene structure and motif distribution of the three unknown groups implied that the genes of the three groups may be functionally different. Expression profiling indicated that the HvbZIP genes exhibited different patterns of spatial and temporal expression. Using qRT-PCR, more than 10 HvbZIP genes were identified with expression patterns similar to those of starch synthase genes in barley. Yeast one-hybrid analysis revealed that two of the HvbZIP genes exhibited in vitro binding activity to the promoter of HvAGP-S. The two HvbZIP genes may be candidate genes for further study to explore the mechanism by which they regulate the synthesis of barley starch.
Xiaojuan Zhong; Xiuqin Feng; Yulong Li; Carlos Guzmán; Na Lin; Qiang Xu; Yazhou Zhang; Huaping Tang; Pengfei Qi; Mei Deng; Jian Ma; Jirui Wang; Guoyue Chen; Xiujin Lan; Yuming Wei; Youliang Zheng; Qiantao Jiang. Genome-wide identification of bZIP transcription factor genes related to starch synthesis in barley (Hordeum vulgare L.). Genome 2021, 1 -14.
AMA StyleXiaojuan Zhong, Xiuqin Feng, Yulong Li, Carlos Guzmán, Na Lin, Qiang Xu, Yazhou Zhang, Huaping Tang, Pengfei Qi, Mei Deng, Jian Ma, Jirui Wang, Guoyue Chen, Xiujin Lan, Yuming Wei, Youliang Zheng, Qiantao Jiang. Genome-wide identification of bZIP transcription factor genes related to starch synthesis in barley (Hordeum vulgare L.). Genome. 2021; ():1-14.
Chicago/Turabian StyleXiaojuan Zhong; Xiuqin Feng; Yulong Li; Carlos Guzmán; Na Lin; Qiang Xu; Yazhou Zhang; Huaping Tang; Pengfei Qi; Mei Deng; Jian Ma; Jirui Wang; Guoyue Chen; Xiujin Lan; Yuming Wei; Youliang Zheng; Qiantao Jiang. 2021. "Genome-wide identification of bZIP transcription factor genes related to starch synthesis in barley (Hordeum vulgare L.)." Genome , no. : 1-14.
The spikelet number per spike (SNS) is a primary factor determining wheat yield. Common wheat breeding reduces the genetic diversity among elite germplasm resources, leading to a detrimental effect on future wheat production. It is, therefore, necessary to explore new genetic resources for SNS to increase wheat yield. A tetraploid landrace ‘Ailanmai’ × wild emmer wheat recombinant inbred line (RIL) population was used to construct a genetic map using the wheat 55K single nucleotide polymorphism (SNP) array. The linkage map containing 1150 bin markers with a total genetic distance of 2411.8 cM was obtained. Based on phenotypic data from eight environments and best linear unbiased prediction (BLUP) values, five quantitative trait loci (QTL) for SNS were identified, explaining 6.71-29.40% of the phenotypic variation. Two of them, QSns.sau-AM-2B.3 and QSns.sau-AM-3B.2, were detected as the major and novel QTL. Their effects were further validated in two additional F2 populations using tightly linked Kompetitive Allele-Specific PCR (KASP) markers. Potential candidate genes within the physical intervals of corresponding QTL were predicted to participate in inflorescence development and spikelet formation. Genetic associations between SNS and other agronomic traits were also detected and analyzed. This study demonstrates the feasibility of wheat 55K SNP array developed for common wheat in genetic mapping of tetraploid population and shows the potential application of wheat related species in wheat improvement programs.
Ziqiang Mo; Jing Zhu; Jiatai Wei; Jieguang Zhou; Qiang Xu; Huaping Tang; Yang Mu; Mei Deng; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Jirui Wang; Pengfei Qi; Wei Li; Yuming Wei; Youliang Zheng; Xiujin Lan; Jian Ma. The 55K SNP-Based Exploration of QTL for Spikelet Number Per Spike in a Tetraploid Wheat (Triticum turgidum L.) Population: Chinese Landrace ‘Ailanmai’ × Wild Emmer. 2020, 1 .
AMA StyleZiqiang Mo, Jing Zhu, Jiatai Wei, Jieguang Zhou, Qiang Xu, Huaping Tang, Yang Mu, Mei Deng, Qiantao Jiang, Yaxi Liu, Guoyue Chen, Jirui Wang, Pengfei Qi, Wei Li, Yuming Wei, Youliang Zheng, Xiujin Lan, Jian Ma. The 55K SNP-Based Exploration of QTL for Spikelet Number Per Spike in a Tetraploid Wheat (Triticum turgidum L.) Population: Chinese Landrace ‘Ailanmai’ × Wild Emmer. . 2020; ():1.
Chicago/Turabian StyleZiqiang Mo; Jing Zhu; Jiatai Wei; Jieguang Zhou; Qiang Xu; Huaping Tang; Yang Mu; Mei Deng; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Jirui Wang; Pengfei Qi; Wei Li; Yuming Wei; Youliang Zheng; Xiujin Lan; Jian Ma. 2020. "The 55K SNP-Based Exploration of QTL for Spikelet Number Per Spike in a Tetraploid Wheat (Triticum turgidum L.) Population: Chinese Landrace ‘Ailanmai’ × Wild Emmer." , no. : 1.
A novel, stably expressed, and plant height-independent QTL for spike extension length on 5AS was identified and validated in different populations using a newly developed and tightly linked KASP marker. As an important component of plant height (PH), spike extension length (SEL) plays a significant role in formation of an ideotype in wheat. Despite the fact that numerous loci for SEL in wheat have been reported, our knowledge on PH-independent loci remains to be limited. In this study, two recombinant inbred line (RIL) populations genotyped using the Wheat55K SNP were used to detect quantitative trait loci (QTL) controlling SEL across six environments. A total of 30 QTL for SEL were detected in these two RIL populations, and four of them, i.e., QSEL.sicau-2CN-4D, QSEL.sicau-2SY-4B.2, QSEL.sicau-2SY-4D.1, and QSEL.sicau-2CN-5A, were stably expressed. Genetic and conditional QTL analysis showed that the first three were significantly associated with PH, while the last one, QSEL.sicau-2CN-5A, is independent of PH. Comparison of genetic and physical maps suggested that only QSEL.sicau-2CN-5A located on chromosome arm 5AS is likely a novel QTL. A Kompetitive Allele-Specific PCR (KASP) marker, KASP-AX-110413733, tightly linked to this novel QTL was developed to successfully confirm its effect in three different genetic populations. Further, in the interval where QSEL.sicau-2CN-5A was located on ‘Chinese Spring’ wheat reference genome, three promising genes mainly expressed in wheat stem were predicated and they all encode the cytochrome P450 that was demonstrated to be closely associated with SEL elongation in rice. In addition, significant correlations between SEL and PH, spikelet number per spike, and thousand-grain weight were also detected. Altogether, our results broaden our understanding on genetic basis of SEL and will be useful for marker-based selection of lines with different SELs and fine mapping the novel and PH-independent QTL QSEL.sicau-2CN-5A.
Cong Li; Huaping Tang; Wei Luo; Xuemei Zhang; Yang Mu; Mei Deng; Yaxi Liu; Qiantao Jiang; Guoyue Chen; Jirui Wang; Pengfei Qi; Zhien Pu; Yunfeng Jiang; Yuming Wei; Youliang Zheng; Xiujin Lan; Jian Ma. A novel, validated, and plant height-independent QTL for spike extension length is associated with yield-related traits in wheat. Theoretical and Applied Genetics 2020, 133, 3381 -3393.
AMA StyleCong Li, Huaping Tang, Wei Luo, Xuemei Zhang, Yang Mu, Mei Deng, Yaxi Liu, Qiantao Jiang, Guoyue Chen, Jirui Wang, Pengfei Qi, Zhien Pu, Yunfeng Jiang, Yuming Wei, Youliang Zheng, Xiujin Lan, Jian Ma. A novel, validated, and plant height-independent QTL for spike extension length is associated with yield-related traits in wheat. Theoretical and Applied Genetics. 2020; 133 (12):3381-3393.
Chicago/Turabian StyleCong Li; Huaping Tang; Wei Luo; Xuemei Zhang; Yang Mu; Mei Deng; Yaxi Liu; Qiantao Jiang; Guoyue Chen; Jirui Wang; Pengfei Qi; Zhien Pu; Yunfeng Jiang; Yuming Wei; Youliang Zheng; Xiujin Lan; Jian Ma. 2020. "A novel, validated, and plant height-independent QTL for spike extension length is associated with yield-related traits in wheat." Theoretical and Applied Genetics 133, no. 12: 3381-3393.
Hairiness, which is a phenotypic trait common among land plants, primarily affects the stem, leaf, and floral organs. Plant hairiness is associated with complex functions. For example, glume hairiness in wheat is related to the resistance to biotic and abiotic stresses, and may also influence human health. In the present study, two pairs of near-isogenic lines (NILs) for glume hairiness, which were derived from a cross between a Tibetan semi-wild wheat accession (Triticum aestivum ssp. tibetanum Q1028) and a common wheat cultivar (T. aestivum ‘Zhengmai 9023’), underwent a glume transcriptome analysis. We detected 27,935 novel genes, of which 18,027 were annotated. Additionally, 488 and 600 differentially expressed genes (DEGs) were detected in NIL1 and NIL2, respectively, with 37 DEGs detected in both NIL pairs. Moreover, 987 and 1584 single nucleotide polymorphisms (SNPs) were detected in NIL1 and NIL2, respectively, with 39 SNPs detected in both NIL pairs, of which most were located in the Hairy glume (Hg) gene region on chromosome arm 1AS. The annotation of the DEGs with gene ontology terms revealed that genes associated with hairiness in Arabidopsis and rice were similarly enriched. The possible functions of these genes related to glume hairiness were examined. The study results provide useful information for identifying candidate genes and the fine-mapping of Hg in the wheat genome.
Wei Luo; Jiajun Liu; Puyang Ding; Cong Li; Hang Liu; Yang Mu; Huaping Tang; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Guangdeng Chen; Yunfeng Jiang; Pengfei Qi; Youliang Zheng; Yuming Wei; Chunji Liu; Xiujin Lan; Jian Ma. Transcriptome analysis of near-isogenic lines for glume hairiness of wheat. Gene 2020, 739, 144517 .
AMA StyleWei Luo, Jiajun Liu, Puyang Ding, Cong Li, Hang Liu, Yang Mu, Huaping Tang, Qiantao Jiang, Yaxi Liu, Guoyue Chen, Guangdeng Chen, Yunfeng Jiang, Pengfei Qi, Youliang Zheng, Yuming Wei, Chunji Liu, Xiujin Lan, Jian Ma. Transcriptome analysis of near-isogenic lines for glume hairiness of wheat. Gene. 2020; 739 ():144517.
Chicago/Turabian StyleWei Luo; Jiajun Liu; Puyang Ding; Cong Li; Hang Liu; Yang Mu; Huaping Tang; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Guangdeng Chen; Yunfeng Jiang; Pengfei Qi; Youliang Zheng; Yuming Wei; Chunji Liu; Xiujin Lan; Jian Ma. 2020. "Transcriptome analysis of near-isogenic lines for glume hairiness of wheat." Gene 739, no. : 144517.
Barley shrunken endosperm mutants have been extensively reported. However, knowledge of the underlying molecular mechanisms of these mutants remains limited. Here, a pair of near isogenic lines (normal endosperm: Bowman and shrunken endosperm: sex1) was subjected to transcriptome analysis to identify mRNAs and lncRNAs related to endosperm development to further dissect its mechanism of molecular regulation. A total of 2123 (1140 up- and 983 down-regulated) unique differentially expressed genes (DEGs) were detected. Functional analyses showed that these DEGs were mainly involved in starch and sucrose metabolism, biosynthesis of secondary metabolites, and plant hormone signal transduction. A total of 343 unique target genes were identified for 57 differentially expressed lncRNAs (DE lncRNAs). These DE lncRNAs were mainly involved in glycerophospholipid metabolism, starch and sucrose metabolism, hormone signal transduction, and stress response. In addition, key lncRNAs were identified by constructing a co-expression network of the target genes of DE lncRNAs. Transcriptome results suggested that mRNA and lncRNA played a critical role in endosperm development. The shrunken endosperm in barley seems to be closely related to plant hormone signal transduction, starch and sucrose metabolism, and cell apoptosis. This study provides a foundation for fine mapping, elucidates the molecular mechanism of shrunken endosperm mutants, and also provides a reference for further studies of lncRNAs during the grain development of plants.
Yaya Zou; Huaping Tang; Ting Li; Min Sun; Xiangru Qu; Jieguang Zhou; Congcong Yang; Yang Mu; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Guangdeng Chen; Youliang Zheng; Yuming Wei; Xiujin Lan; Jian Ma. Identification and characterization of mRNAs and lncRNAs of a barley shrunken endosperm mutant using RNA-seq. Genetica 2020, 148, 55 -68.
AMA StyleYaya Zou, Huaping Tang, Ting Li, Min Sun, Xiangru Qu, Jieguang Zhou, Congcong Yang, Yang Mu, Qiantao Jiang, Yaxi Liu, Guoyue Chen, Guangdeng Chen, Youliang Zheng, Yuming Wei, Xiujin Lan, Jian Ma. Identification and characterization of mRNAs and lncRNAs of a barley shrunken endosperm mutant using RNA-seq. Genetica. 2020; 148 (2):55-68.
Chicago/Turabian StyleYaya Zou; Huaping Tang; Ting Li; Min Sun; Xiangru Qu; Jieguang Zhou; Congcong Yang; Yang Mu; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Guangdeng Chen; Youliang Zheng; Yuming Wei; Xiujin Lan; Jian Ma. 2020. "Identification and characterization of mRNAs and lncRNAs of a barley shrunken endosperm mutant using RNA-seq." Genetica 148, no. 2: 55-68.
A completely developed and vigorous root system can provide a stable platform for aboveground plant organs. To identify loci controlling root traits that could be used in wheat (Triticum aestivum L.) breeding, 199 recombinant inbred lines were used to measure and analyze eight root traits. A total of 18 quantitative trait loci (QTL) located on chromosomes 1A, 2A, 2B, 2D, 4B, 4D, 6A, 7A, and 7B were identified. The phenotypic variation explained by these 18 QTL ranged from 3.27% to 11.75%, and the logarithm of odds scores ranged from 2.50 to 6.58. A comparison of physical intervals indicated several new QTL for root traits were identified. In addition, significant correlations between root and agronomic traits were detected and discussed. The results presented in this study, along with those of previous reports, suggest that chromosomes 2 and 7 likely play important roles in the growth and development of wheat roots.
Ting Li; Jian Ma; Yaya Zou; Guangdeng Chen; Pu-Yang Ding; Han Zhang; Cong-Cong Yang; Yang Mu; Huaping Tang; Yaxi Liu; Qiantao Jiang; Guo-Yue Chen; Peng-Fei Qi; Yu-Ming Wei; Youliang Zheng; Xiu-Jin Lan. Quantitative trait loci for seeding root traits and the relationships between root and agronomic traits in common wheat. Genome 2020, 63, 27 -36.
AMA StyleTing Li, Jian Ma, Yaya Zou, Guangdeng Chen, Pu-Yang Ding, Han Zhang, Cong-Cong Yang, Yang Mu, Huaping Tang, Yaxi Liu, Qiantao Jiang, Guo-Yue Chen, Peng-Fei Qi, Yu-Ming Wei, Youliang Zheng, Xiu-Jin Lan. Quantitative trait loci for seeding root traits and the relationships between root and agronomic traits in common wheat. Genome. 2020; 63 (1):27-36.
Chicago/Turabian StyleTing Li; Jian Ma; Yaya Zou; Guangdeng Chen; Pu-Yang Ding; Han Zhang; Cong-Cong Yang; Yang Mu; Huaping Tang; Yaxi Liu; Qiantao Jiang; Guo-Yue Chen; Peng-Fei Qi; Yu-Ming Wei; Youliang Zheng; Xiu-Jin Lan. 2020. "Quantitative trait loci for seeding root traits and the relationships between root and agronomic traits in common wheat." Genome 63, no. 1: 27-36.
Unfortunately, the author contribution statement was missed out in the original publication. The complete statement is given below.
Jian Ma; Puyang Ding; Jiajun Liu; Ting Li; Yaya Zou; Ahsan Habib; Yang Mu; Huaping Tang; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Jirui Wang; Mei Deng; Pengfei Qi; Wei Li; Zhien Pu; Youliang Zheng; Yuming Wei; Xiujin Lan. Correction to: Identification and validation of a major and stably expressed QTL for spikelet number per spike in bread wheat. Theoretical and Applied Genetics 2019, 133, 367 -367.
AMA StyleJian Ma, Puyang Ding, Jiajun Liu, Ting Li, Yaya Zou, Ahsan Habib, Yang Mu, Huaping Tang, Qiantao Jiang, Yaxi Liu, Guoyue Chen, Jirui Wang, Mei Deng, Pengfei Qi, Wei Li, Zhien Pu, Youliang Zheng, Yuming Wei, Xiujin Lan. Correction to: Identification and validation of a major and stably expressed QTL for spikelet number per spike in bread wheat. Theoretical and Applied Genetics. 2019; 133 (1):367-367.
Chicago/Turabian StyleJian Ma; Puyang Ding; Jiajun Liu; Ting Li; Yaya Zou; Ahsan Habib; Yang Mu; Huaping Tang; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Jirui Wang; Mei Deng; Pengfei Qi; Wei Li; Zhien Pu; Youliang Zheng; Yuming Wei; Xiujin Lan. 2019. "Correction to: Identification and validation of a major and stably expressed QTL for spikelet number per spike in bread wheat." Theoretical and Applied Genetics 133, no. 1: 367-367.
Fusarium graminearum causes Fusarium head blight (FHB), a devastating disease of wheat. Salicylic acid (SA) is involved in the resistance of wheat to F. graminearum. Cell wall mannoprotein (CWM) is known to trigger defense responses in plants, but its role in the pathogenicity of F. graminearum remains unclear. Here, we characterized FgCWM1 (FG05_11315), encoding a CWM in F. graminearum. FgCWM1 was highly expressed in wheat spikes by 24 h after initial inoculation and was upregulated by SA. Disruption of FgCWM1 (ΔFgCWM1) reduced mannose and protein accumulation in the fungal cell wall, especially under SA treatment, and resulted in defective fungal cell walls, leading to increased fungal sensitivity to SA. The positive role of FgCWM1 in mannose and protein accumulation was confirmed by its expression in Saccharomyces cerevisiae. Compared with wild type (WT), ΔFgCWM1 exhibited reduced pathogenicity toward wheat, but it produced the same amount of deoxynivalenol both in culture and in spikes. Complementation of ΔFgCWM1 with FgCWM1 restored the WT phenotype. Localization analyses revealed that FgCWM1 was distributed on the cell wall, consistent with its structural role. Thus, FgCWM1 encodes a CWM protein that plays an important role in the cell wall integrity and pathogenicity of F. graminearum.
Ya-Zhou Zhang; Qing Chen; Cai-Hong Liu; Lu Lei; Yang Li; Kan Zhao; Mei-Qiao Wei; Zhen-Ru Guo; Yan Wang; Bin-Jie Xu; Yun-Feng Jiang; Li Kong; Yan-Lin Liu; Xiu-Jin Lan; Qian-Tao Jiang; Jian Ma; Ji-Rui Wang; Guo-Yue Chen; Yu-Ming Wei; You-Liang Zheng; Peng-Fei Qi. Fusarium graminearum FgCWM1 Encodes a Cell Wall Mannoprotein Conferring Sensitivity to Salicylic Acid and Virulence to Wheat. Toxins 2019, 11, 628 .
AMA StyleYa-Zhou Zhang, Qing Chen, Cai-Hong Liu, Lu Lei, Yang Li, Kan Zhao, Mei-Qiao Wei, Zhen-Ru Guo, Yan Wang, Bin-Jie Xu, Yun-Feng Jiang, Li Kong, Yan-Lin Liu, Xiu-Jin Lan, Qian-Tao Jiang, Jian Ma, Ji-Rui Wang, Guo-Yue Chen, Yu-Ming Wei, You-Liang Zheng, Peng-Fei Qi. Fusarium graminearum FgCWM1 Encodes a Cell Wall Mannoprotein Conferring Sensitivity to Salicylic Acid and Virulence to Wheat. Toxins. 2019; 11 (11):628.
Chicago/Turabian StyleYa-Zhou Zhang; Qing Chen; Cai-Hong Liu; Lu Lei; Yang Li; Kan Zhao; Mei-Qiao Wei; Zhen-Ru Guo; Yan Wang; Bin-Jie Xu; Yun-Feng Jiang; Li Kong; Yan-Lin Liu; Xiu-Jin Lan; Qian-Tao Jiang; Jian Ma; Ji-Rui Wang; Guo-Yue Chen; Yu-Ming Wei; You-Liang Zheng; Peng-Fei Qi. 2019. "Fusarium graminearum FgCWM1 Encodes a Cell Wall Mannoprotein Conferring Sensitivity to Salicylic Acid and Virulence to Wheat." Toxins 11, no. 11: 628.
Background Kernel length (KL), kernel width (KW) and thousand-kernel weight (TKW) are key agronomic traits in wheat breeding. Chuannong16 (‘CN16’) is a commercial cultivar with significantly longer kernels than the line ‘20828’. To identify and characterize potential alleles from CN16 controlling KL, the previously developed recombinant inbred line (RIL) population derived from the cross ‘20828’ × ‘CN16’ and the genetic map constructed by the Wheat55K SNP array and SSR markers were used to perform quantitative trait locus/loci (QTL) analyses for kernel traits. Results A total of 11 putative QTL associated with kernel traits were identified and they were located on chromosomes 1A (2 QTL), 2B (2 QTL), 2D (3 QTL), 3D, 4A, 6A, and 7A, respectively. Among them, three major QTL, QKL.sicau-2D, QKW.sicau-2D and QTKW.sicau-2D, controlling KL, KW and TKW, respectively, were detected in three different environments. Respectively, they explained 10.88–18.85%, 17.21–21.49% and 10.01–23.20% of the phenotypic variance. Further, they were genetically mapped in the same interval on chromosome 2DS. A previously developed kompetitive allele-specific PCR (KASP) marker KASP-AX-94721936 was integrated in the genetic map and QTL re-mapping finally located the three major QTL in a 1- cM region flanked by AX-111096297 and KASP-AX-94721936. Another two co-located QTL intervals for KL and TKW were also identified. A few predicted genes involved in regulation of kernel growth and development were identified in the intervals of these identified QTL. Significant relationships between kernel traits and spikelet number per spike and anthesis date were detected and discussed. Conclusions Three major and stably expressed QTL associated with KL, KW, and TKW were identified. A KASP marker tightly linked to these three major QTL was integrated. These findings provide information for subsequent fine mapping and cloning the three co-localized major QTL for kernel traits.
Jian Ma; Han Zhang; Shuiqin Li; Yaya Zou; Ting Li; Jiajun Liu; Puyang Ding; Yang Mu; Huaping Tang; Mei Deng; Yaxi Liu; Qiantao Jiang; Guoyue Chen; Houyang Kang; Wei Li; Zhien Pu; Yuming Wei; Youliang Zheng; Xiujin Lan. Identification of quantitative trait loci for kernel traits in a wheat cultivar Chuannong16. BMC Genetics 2019, 20, 1 -12.
AMA StyleJian Ma, Han Zhang, Shuiqin Li, Yaya Zou, Ting Li, Jiajun Liu, Puyang Ding, Yang Mu, Huaping Tang, Mei Deng, Yaxi Liu, Qiantao Jiang, Guoyue Chen, Houyang Kang, Wei Li, Zhien Pu, Yuming Wei, Youliang Zheng, Xiujin Lan. Identification of quantitative trait loci for kernel traits in a wheat cultivar Chuannong16. BMC Genetics. 2019; 20 (1):1-12.
Chicago/Turabian StyleJian Ma; Han Zhang; Shuiqin Li; Yaya Zou; Ting Li; Jiajun Liu; Puyang Ding; Yang Mu; Huaping Tang; Mei Deng; Yaxi Liu; Qiantao Jiang; Guoyue Chen; Houyang Kang; Wei Li; Zhien Pu; Yuming Wei; Youliang Zheng; Xiujin Lan. 2019. "Identification of quantitative trait loci for kernel traits in a wheat cultivar Chuannong16." BMC Genetics 20, no. 1: 1-12.
A major and stably expressed QTL for spikelet number per spike identified in a 2-cM interval on chromosome arm 2DS was validated using two populations with different genetic backgrounds. Spikelet number per spike (SNS) plays a key role in wheat yield improvement. Numerous genetic and environmental factors influencing SNS have been documented, but the number of major, stably expressed and validated loci underlying SNS is still limited. In this study, a recombinant inbred line (RIL) population derived from a normal spikelet cultivar and a multiple-spikelet wheat line (with a longer spike with more canonically oriented apical spikelets) was genotyped using a Wheat55K single-nucleotide polymorphism (SNP) array and simple sequence repeat (SSR) markers. SNS was measured for this RIL population in eight environments. Five QTL were each identified in two or more environments. One of them, QSns.sau-2D (LOD = 3.47–38.24, PVE = 10.16–45.68%), was detected in all the eight environments. The QTL was located in a 2-cM interval on chromosome arm 2DS flanked by the markers AX-109836946 and AX-111956072. This QTL, QSns.sau-2D, significantly increased SNS by up to 14.72%. Several genes associated with plant growth and development were identified in the physical interval of QSns.sau-2D. This QTL was further validated by the tightly linked Kompetitive Allele Specific PCR (KASP) marker, KASP-AX-94721936, in two other populations with different genetic backgrounds. The significant correlation between SNS and anthesis date, plant height, spike length, grain number per spike and thousand-grain weight were detected and discussed. These results lay the foundation for fine mapping and cloning gene(s) underlying QSns.sau-2D.
Jian Ma; Puyang Ding; Jiajun Liu; Ting Li; Yaya Zou; Ahsan Habib; Yang Mu; Huaping Tang; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Jirui Wang; Mei Deng; Pengfei Qi; Wei Li; Zhien Pu; Youliang Zheng; Yuming Wei; Xiujin Lan. Identification and validation of a major and stably expressed QTL for spikelet number per spike in bread wheat. Theoretical and Applied Genetics 2019, 132, 3155 -3167.
AMA StyleJian Ma, Puyang Ding, Jiajun Liu, Ting Li, Yaya Zou, Ahsan Habib, Yang Mu, Huaping Tang, Qiantao Jiang, Yaxi Liu, Guoyue Chen, Jirui Wang, Mei Deng, Pengfei Qi, Wei Li, Zhien Pu, Youliang Zheng, Yuming Wei, Xiujin Lan. Identification and validation of a major and stably expressed QTL for spikelet number per spike in bread wheat. Theoretical and Applied Genetics. 2019; 132 (11):3155-3167.
Chicago/Turabian StyleJian Ma; Puyang Ding; Jiajun Liu; Ting Li; Yaya Zou; Ahsan Habib; Yang Mu; Huaping Tang; Qiantao Jiang; Yaxi Liu; Guoyue Chen; Jirui Wang; Mei Deng; Pengfei Qi; Wei Li; Zhien Pu; Youliang Zheng; Yuming Wei; Xiujin Lan. 2019. "Identification and validation of a major and stably expressed QTL for spikelet number per spike in bread wheat." Theoretical and Applied Genetics 132, no. 11: 3155-3167.
Phytohormones are key regulators of plant growth, development, and signalling networks involved in responses to diverse biotic and abiotic stresses. Transcriptional reference maps of hormone responses have been reported for several model plant species such as Arabidopsis thaliana, Oryza sativa, and Brachypodium distachyon. However, because of species differences and the complexity of the wheat genome, these transcriptome data are not appropriate reference material for wheat studies. We comprehensively analysed the transcriptomic responses in wheat spikes to seven phytohormones, including indole acetic acid (IAA), gibberellic acid (GA), abscisic acid (ABA), ethylene (ET), cytokinin (CK), salicylic acid (SA), and methyl jasmonic acid (MeJA). A total of 3386 genes were differentially expressed at 24 h after the hormone treatments. Furthermore, 22.7% of these genes exhibited overlapping transcriptional responses for at least two hormones, implying there is crosstalk among phytohormones. We subsequently identified genes with expression levels that were significantly and differentially induced by a specific phytohormone (i.e., hormone-specific responses). The data for these hormone-responsive genes were then compared with the transcriptome data for wheat spikes exposed to biotic (Fusarium head blight) and abiotic (water deficit) stresses. Our data were used to develop a transcriptional reference map of hormone responses in wheat spikes.
Peng-Fei Qi; Yun-Feng Jiang; Zhen-Ru Guo; Qing Chen; Thérèse Ouellet; Lu-Juan Zong; Zhen-Zhen Wei; Yan Wang; Ya-Zhou Zhang; Bin-Jie Xu; Li Kong; Mei Deng; Ji-Rui Wang; Guo-Yue Chen; Qian-Tao Jiang; Xiu-Jin Lan; Wei Li; Yu-Ming Wei; You-Liang Zheng. Transcriptional reference map of hormone responses in wheat spikes. BMC Genomics 2019, 20, 390 .
AMA StylePeng-Fei Qi, Yun-Feng Jiang, Zhen-Ru Guo, Qing Chen, Thérèse Ouellet, Lu-Juan Zong, Zhen-Zhen Wei, Yan Wang, Ya-Zhou Zhang, Bin-Jie Xu, Li Kong, Mei Deng, Ji-Rui Wang, Guo-Yue Chen, Qian-Tao Jiang, Xiu-Jin Lan, Wei Li, Yu-Ming Wei, You-Liang Zheng. Transcriptional reference map of hormone responses in wheat spikes. BMC Genomics. 2019; 20 (1):390.
Chicago/Turabian StylePeng-Fei Qi; Yun-Feng Jiang; Zhen-Ru Guo; Qing Chen; Thérèse Ouellet; Lu-Juan Zong; Zhen-Zhen Wei; Yan Wang; Ya-Zhou Zhang; Bin-Jie Xu; Li Kong; Mei Deng; Ji-Rui Wang; Guo-Yue Chen; Qian-Tao Jiang; Xiu-Jin Lan; Wei Li; Yu-Ming Wei; You-Liang Zheng. 2019. "Transcriptional reference map of hormone responses in wheat spikes." BMC Genomics 20, no. 1: 390.
It is reported that GLW7 encoding the transcription factor OsSPL13, positively regulates grain size and shape in rice. We have limited knowledge about its orthologs in wheat. Here, based on the rice OsGLW7 we isolated and identified the TaGLW7 gene in wheat, characterized its nucleotide and protein structures, predicted the cis-elements of its promoter, analyzed its expression patterns. The orthologs in barley (HvGLW7), Brachypodium (BdGLW7), wild emmer (TtGLW7), Aegilops tauschii (AtGLW7) were also used for comparative analysis. As predicated, TaGLW7, HvGLW7, TtGLW7, and AtGLW7 were mapped onto group 2 chromosomes in the respective species. Multiple alignments indicated GLW7 possesses two exons and one intron in the analyzed species. GLW7 contains a conserved domain SBP and two neighboring low complexity regions. GLW7 was highly expressed in spike organs including wheat young spikes, barley inflorescence, and rice anthers. Additionally, biotic stress significantly down-regulated GLW7 in wheat and barley. Significant correlations between the expression patterns of predicted transcription factor ABF2 and TaGLW7 were detected. In conclusion, the conserved structure and expression of GLW7 among the investigated species and the predicted transcription factors significantly related to GLW7 are helpful for further manipulating GLW7 and uncovering its roles in plants.
C. C. Yang; J. Ma; T. Li; W. Luo; Y. Mu; H. P. Tang; X. J. Lan. Structural Organization and Functional Activity of the Orthologous TaGLW7 Genes in Bread Wheat (Triticum aestivum L.). Russian Journal of Genetics 2019, 55, 571 -579.
AMA StyleC. C. Yang, J. Ma, T. Li, W. Luo, Y. Mu, H. P. Tang, X. J. Lan. Structural Organization and Functional Activity of the Orthologous TaGLW7 Genes in Bread Wheat (Triticum aestivum L.). Russian Journal of Genetics. 2019; 55 (5):571-579.
Chicago/Turabian StyleC. C. Yang; J. Ma; T. Li; W. Luo; Y. Mu; H. P. Tang; X. J. Lan. 2019. "Structural Organization and Functional Activity of the Orthologous TaGLW7 Genes in Bread Wheat (Triticum aestivum L.)." Russian Journal of Genetics 55, no. 5: 571-579.
A major, likely novel stripe rust resistance QTL for all-stage resistance on chromosome arm 1BL identified in a 1.76-cM interval using a saturated linkage map was validated in four populations with different genetic backgrounds. Stripe rust is a globally important disease of wheat. Identification and utilization of new resistance genes are essential for breeding resistant cultivars. Wheat line 20828 has exhibited high levels of stripe rust resistance for over a decade. However, the genetics of stripe rust resistance in this line has not been studied. A set of 199 recombinant inbred lines (RILs) were developed from a cross between 20828 and a susceptible cultivar Chuannong 16. The RIL population was genotyped with the Wheat55K SNP (single nucleotide polymorphism) array and SSR (simple sequence repeat) markers and evaluated in four environments with current predominant Puccinia striiformis f. sp. tritici t races including CYR32, CYR33 and CYR34. Four stable QTL were located on chromosomes 1B (2 QTL), 4A and 6A. Among them, the major QTL, QYr.sicau-1B.1 (LOD = 23–28, PVE = 16–39%), was localized to a 1.76-cM interval flanked by SSR markers Xwmc216 and Xwmc156 on chromosome 1BL. Eight resistance genes were previously identified in the physical interval of QYr.sicau-1B.1. Compared with previous studies, QYr.sicau-1B.1 is a new gene for resistant to stripe rust. It was further verified by analysis of the closely linked SSR markers Xwmc216 and Xwmc156 in four other populations with different genetic backgrounds. QYr.sicau-1B.1 reduced the stripe rust disease index by up to 82.8%. Three minor stable QTL (located on chromosomes 1B, 4A and 6A, respectively) also added to the resistance level of QYr.sicau-1B.1. Our results provide valuable information for further fine mapping and cloning as well as molecular-assisted breeding with QYr.sicau-1B.1.
Jian Ma; Nana Qin; Ben Cai; Guoyue Chen; Puyang Ding; Han Zhang; Congcong Yang; Lin Huang; Yang Mu; Huaping Tang; Yaxi Liu; Jirui Wang; Pengfei Qi; Qiantao Jiang; Youliang Zheng; Chunji Liu; Xiujin Lan; Yuming Wei. Identification and validation of a novel major QTL for all-stage stripe rust resistance on 1BL in the winter wheat line 20828. Theoretical and Applied Genetics 2019, 132, 1363 -1373.
AMA StyleJian Ma, Nana Qin, Ben Cai, Guoyue Chen, Puyang Ding, Han Zhang, Congcong Yang, Lin Huang, Yang Mu, Huaping Tang, Yaxi Liu, Jirui Wang, Pengfei Qi, Qiantao Jiang, Youliang Zheng, Chunji Liu, Xiujin Lan, Yuming Wei. Identification and validation of a novel major QTL for all-stage stripe rust resistance on 1BL in the winter wheat line 20828. Theoretical and Applied Genetics. 2019; 132 (5):1363-1373.
Chicago/Turabian StyleJian Ma; Nana Qin; Ben Cai; Guoyue Chen; Puyang Ding; Han Zhang; Congcong Yang; Lin Huang; Yang Mu; Huaping Tang; Yaxi Liu; Jirui Wang; Pengfei Qi; Qiantao Jiang; Youliang Zheng; Chunji Liu; Xiujin Lan; Yuming Wei. 2019. "Identification and validation of a novel major QTL for all-stage stripe rust resistance on 1BL in the winter wheat line 20828." Theoretical and Applied Genetics 132, no. 5: 1363-1373.
Salicylic acid (SA) is a key defense hormone associated with wheat resistance against Fusarium head blight, which is a severe disease mainly caused by Fusarium graminearum. Although F. graminearum can metabolize SA, it remains unclear how this metabolic activity affects the wheat–F. graminearum interaction. In this study, we identified a salicylate hydroxylase gene (FG05_08116; FgNahG) in F. graminearum. This gene encodes a protein that catalyzes the conversion of SA to catechol. Additionally, FgNahG was widely distributed within hyphae. Disrupting the FgNahG gene (ΔFgNahG) led to enhanced sensitivity to SA, increased accumulation of SA in wheat spikes during the early infection stage and inhibited development of head blight symptoms. However, FgNahG did not affect mycotoxin production. Re-introducing a functional FgNahG gene into the ΔFgNahG mutant recovered the wild-type phenotype. Moreover, the expression of FgNahG in transgenic Arabidopsis thaliana decreased the SA concentration and the resistance of leaves to F. graminearum. These results indicate that the endogenous SA in wheat influences the resistance against F. graminearum. Furthermore, the capacity to metabolize SA is an important factor affecting the ability of F. graminearum to infect wheat plants.
Peng-Fei Qi; Ya-Zhou Zhang; Cai-Hong Liu; Qing Chen; Zhen-Ru Guo; Yan Wang; Bin-Jie Xu; Yun-Feng Jiang; Ting Zheng; Xi Gong; Cui-Hua Luo; Wang Wu; Li Kong; Mei Deng; Jian Ma; Xiu-Jin Lan; Qian-Tao Jiang; Yu-Ming Wei; Ji-Rui Wang; You-Liang Zheng. Functional Analysis of FgNahG Clarifies the Contribution of Salicylic Acid to Wheat (Triticum aestivum) Resistance against Fusarium Head Blight. Toxins 2019, 11, 59 .
AMA StylePeng-Fei Qi, Ya-Zhou Zhang, Cai-Hong Liu, Qing Chen, Zhen-Ru Guo, Yan Wang, Bin-Jie Xu, Yun-Feng Jiang, Ting Zheng, Xi Gong, Cui-Hua Luo, Wang Wu, Li Kong, Mei Deng, Jian Ma, Xiu-Jin Lan, Qian-Tao Jiang, Yu-Ming Wei, Ji-Rui Wang, You-Liang Zheng. Functional Analysis of FgNahG Clarifies the Contribution of Salicylic Acid to Wheat (Triticum aestivum) Resistance against Fusarium Head Blight. Toxins. 2019; 11 (2):59.
Chicago/Turabian StylePeng-Fei Qi; Ya-Zhou Zhang; Cai-Hong Liu; Qing Chen; Zhen-Ru Guo; Yan Wang; Bin-Jie Xu; Yun-Feng Jiang; Ting Zheng; Xi Gong; Cui-Hua Luo; Wang Wu; Li Kong; Mei Deng; Jian Ma; Xiu-Jin Lan; Qian-Tao Jiang; Yu-Ming Wei; Ji-Rui Wang; You-Liang Zheng. 2019. "Functional Analysis of FgNahG Clarifies the Contribution of Salicylic Acid to Wheat (Triticum aestivum) Resistance against Fusarium Head Blight." Toxins 11, no. 2: 59.
DNA methylation is dynamically involved in plant immunity, but little information is known about its roles in plant interactions with biotrophic fungi, especially in temperate grasses such as wheat (Triticum aestivum).Using wheat diploid progenitor Aegilops tauschii accession AL8/78, the genome of which has been sequenced, we assessed the extent of DNA methylation in response to infection with Blumeria graminis f. sp. tritici (Bgt), which causes powdery mildew.Upon Bgt infection, ARGONAUTE4a (AGO4a) was significantly downregulated in A. tauschii, which was accompanied by a substantial reduction in AGO4a‐sorted 24‐nt siRNA levels, especially for genes near transposable elements (TAGs). Bisulfite sequencing revealed abundant differentially methylated regions (DMRs) with CHH hypomethylation. TAGs bearing CHH‐hypomethylated DMRs were enriched for ‘response to stress’ functions, including receptor kinase, peroxidase, and pathogenesis‐related genes. Virus‐induced gene silencing (VIGS) of a DOMAINS REARRANGED METHYLASE 2 (DRM2) homolog enhanced plant resistance to Bgt. The effect of CHH hypomethylation was exemplified by the upregulation of a pathogenesis‐related β‐1,3‐glucanse gene implicated in Bgt defense.These findings support the idea that dynamic DNA methylation represents a regulatory layer in the complex mechanism of plant immunity, which could be exploited to improve disease resistance in common wheat.
Shuaifeng Geng; Xingchen Kong; Gaoyuan Song; Meiling Jia; Jiantao Guan; Fang Wang; Zhengrui Qin; Liang Wu; Xiujin Lan; Aili Li; Long Mao. DNA methylation dynamics during the interaction of wheat progenitor Aegilops tauschii with the obligate biotrophic fungus Blumeria graminis f. sp. tritici. New Phytologist 2018, 221, 1023 -1035.
AMA StyleShuaifeng Geng, Xingchen Kong, Gaoyuan Song, Meiling Jia, Jiantao Guan, Fang Wang, Zhengrui Qin, Liang Wu, Xiujin Lan, Aili Li, Long Mao. DNA methylation dynamics during the interaction of wheat progenitor Aegilops tauschii with the obligate biotrophic fungus Blumeria graminis f. sp. tritici. New Phytologist. 2018; 221 (2):1023-1035.
Chicago/Turabian StyleShuaifeng Geng; Xingchen Kong; Gaoyuan Song; Meiling Jia; Jiantao Guan; Fang Wang; Zhengrui Qin; Liang Wu; Xiujin Lan; Aili Li; Long Mao. 2018. "DNA methylation dynamics during the interaction of wheat progenitor Aegilops tauschii with the obligate biotrophic fungus Blumeria graminis f. sp. tritici." New Phytologist 221, no. 2: 1023-1035.
The translocation of 4AL/5AL in Triticum, which occurred before the differentiation of T. urartu and einkorn, is an important chromosomal rearrangement. Recently, the first identification of breakpoint sequence on 4AL for this translocation provides the opportunity to analyze the variation and diversity of breakpoints in Triticum. In this study, the breakpoint regions of 52 accessions from 21 species were isolated and further characterized. The sequences were divided into 12 types based on their lengths, which ranged from 2009 to 2552 bp. Cluster analysis showed that they were further divided into three groups. Interesting evolutionary relationships among a few of the species were observed and discussed. Multiple sequence alignment of the 52 sequences made it possible to detect 13 insertion and deletion length polymorphisms (InDels) and 101 single nucleotide polymorphisms (SNPs). Furthermore, several species- or accession-specific SNPs or InDels were also identified. Based on BLAST analysis of the conserved sequences, the breakpoint was narrowed down to a 125 bp fragment. Taken together, the results obtained in this study enrich our understanding of chromosomal breakpoints and will be useful for the identification of other breakpoints in wheat.
Wei Luo; Na-Na Qin; Yang Mu; Huaping Tang; Mei Deng; Yaxi Liu; Guangdeng Chen; Qian-Tao Jiang; Guo-Yue Chen; Yuming Wei; Youliang Zheng; Xiu-Jin Lan; Jian Ma. Variation and diversity of the breakpoint sequences on 4AL for the 4AL/5AL translocation in Triticum. Genome 2018, 61, 635 -641.
AMA StyleWei Luo, Na-Na Qin, Yang Mu, Huaping Tang, Mei Deng, Yaxi Liu, Guangdeng Chen, Qian-Tao Jiang, Guo-Yue Chen, Yuming Wei, Youliang Zheng, Xiu-Jin Lan, Jian Ma. Variation and diversity of the breakpoint sequences on 4AL for the 4AL/5AL translocation in Triticum. Genome. 2018; 61 (9):635-641.
Chicago/Turabian StyleWei Luo; Na-Na Qin; Yang Mu; Huaping Tang; Mei Deng; Yaxi Liu; Guangdeng Chen; Qian-Tao Jiang; Guo-Yue Chen; Yuming Wei; Youliang Zheng; Xiu-Jin Lan; Jian Ma. 2018. "Variation and diversity of the breakpoint sequences on 4AL for the 4AL/5AL translocation in Triticum." Genome 61, no. 9: 635-641.
A high-density genetic map constructed with a wheat 55 K SNP array was highly consistent with the physical map of this species and it facilitated the identification of a novel major QTL for productive tiller number. Productive tiller number (PTN) plays a key role in wheat grain yield. In this study, a recombinant inbred line population with 199 lines derived from a cross between ‘20828’ and ‘Chuannong16’ was used to construct a high-density genetic map using wheat 55 K single nucleotide polymorphism (SNP) array. The constructed genetic map contains 12,109 SNP markers spanning 3021.04 cM across the 21 wheat chromosomes. The orders of the genetic and physical positions of these markers are generally in agreement, and they also match well with those based on the 660 K SNP array from which the one used in this study was derived. The ratios of SNPs located in each of the wheat deletion bins were similar among the wheat 9 K, 55 K, 90 K, 660 K and 820 K SNP arrays. Based on the constructed maps, a novel major quantitative trait locus QPtn.sau-4B for PTN was detected across multi-environments in a 0.55 cM interval on 4B and it explained 17.23–45.46% of the phenotypic variance. Twenty common genes in the physical interval between the flanking markers were identified on chromosome 4B of ‘Chinese Spring’ and wild emmer. These results indicate that wheat 55 K SNP array could be an ideal tool in primary mapping of target genes and the identification of QPtn.sau-4B laid a foundation for the following fine mapping and cloning work.
Jiajun Liu; Wei Luo; Nana Qin; Puyang Ding; Han Zhang; Congcong Yang; Yang Mu; Huaping Tang; Yaxi Liu; Wei Li; Qiantao Jiang; Guoyue Chen; Yuming Wei; Youliang Zheng; Chunji Liu; Xiujin Lan; Jian Ma. A 55 K SNP array-based genetic map and its utilization in QTL mapping for productive tiller number in common wheat. Theoretical and Applied Genetics 2018, 131, 2439 -2450.
AMA StyleJiajun Liu, Wei Luo, Nana Qin, Puyang Ding, Han Zhang, Congcong Yang, Yang Mu, Huaping Tang, Yaxi Liu, Wei Li, Qiantao Jiang, Guoyue Chen, Yuming Wei, Youliang Zheng, Chunji Liu, Xiujin Lan, Jian Ma. A 55 K SNP array-based genetic map and its utilization in QTL mapping for productive tiller number in common wheat. Theoretical and Applied Genetics. 2018; 131 (11):2439-2450.
Chicago/Turabian StyleJiajun Liu; Wei Luo; Nana Qin; Puyang Ding; Han Zhang; Congcong Yang; Yang Mu; Huaping Tang; Yaxi Liu; Wei Li; Qiantao Jiang; Guoyue Chen; Yuming Wei; Youliang Zheng; Chunji Liu; Xiujin Lan; Jian Ma. 2018. "A 55 K SNP array-based genetic map and its utilization in QTL mapping for productive tiller number in common wheat." Theoretical and Applied Genetics 131, no. 11: 2439-2450.
ATP-binding cassette (ABC) transporters hydrolyze ATP to transport a wide range of substrates. Fusarium graminearum is a major causal agent of Fusarium head blight, which is a severe disease in wheat worldwide. FgABCC9 (FG05_07325) encodes an ABC-C (ABC transporter family C) transporter in F. graminearum, which was highly expressed during the infection in wheat and was up-regulated by the plant defense hormone salicylic acid (SA) and the fungicide tebuconazole. The predicted tertiary structure of the FgABCC9 protein was consistent with the schematic of the ABC exporter. Deletion of FgABCC9 resulted in decreased mycelial growth, increased sensitivity to SA and tebuconazole, reduced accumulation of deoxynivalenol (DON), and less pathogenicity towards wheat. Re-introduction of a functional FgABCC9 gene into ΔFgABCC9 recovered the phenotypes of the wild type strain. Transgenic expression of FgABCC9 in Arabidopsis thaliana increased the accumulation of SA in its leaves without activating SA signaling, which suggests that FgABCC9 functions as an SA exporter. Taken together, FgABCC9 encodes an ABC exporter, which is critical for fungal exportation of SA, response to tebuconazole, mycelial growth, and pathogenicity towards wheat.
Peng-Fei Qi; Ya-Zhou Zhang; Cai-Hong Liu; Jing Zhu; Qing Chen; Zhen-Ru Guo; Yan Wang; Bin-Jie Xu; Ting Zheng; Yun-Feng Jiang; Jiang-Ping Wang; Cai-Yi Zhou; Xiang Feng; Li Kong; Xiu-Jin Lan; Qian-Tao Jiang; Yu-Ming Wei; You-Liang Zheng. Fusarium graminearum ATP-Binding Cassette Transporter Gene FgABCC9 Is Required for Its Transportation of Salicylic Acid, Fungicide Resistance, Mycelial Growth and Pathogenicity towards Wheat. International Journal of Molecular Sciences 2018, 19, 2351 .
AMA StylePeng-Fei Qi, Ya-Zhou Zhang, Cai-Hong Liu, Jing Zhu, Qing Chen, Zhen-Ru Guo, Yan Wang, Bin-Jie Xu, Ting Zheng, Yun-Feng Jiang, Jiang-Ping Wang, Cai-Yi Zhou, Xiang Feng, Li Kong, Xiu-Jin Lan, Qian-Tao Jiang, Yu-Ming Wei, You-Liang Zheng. Fusarium graminearum ATP-Binding Cassette Transporter Gene FgABCC9 Is Required for Its Transportation of Salicylic Acid, Fungicide Resistance, Mycelial Growth and Pathogenicity towards Wheat. International Journal of Molecular Sciences. 2018; 19 (8):2351.
Chicago/Turabian StylePeng-Fei Qi; Ya-Zhou Zhang; Cai-Hong Liu; Jing Zhu; Qing Chen; Zhen-Ru Guo; Yan Wang; Bin-Jie Xu; Ting Zheng; Yun-Feng Jiang; Jiang-Ping Wang; Cai-Yi Zhou; Xiang Feng; Li Kong; Xiu-Jin Lan; Qian-Tao Jiang; Yu-Ming Wei; You-Liang Zheng. 2018. "Fusarium graminearum ATP-Binding Cassette Transporter Gene FgABCC9 Is Required for Its Transportation of Salicylic Acid, Fungicide Resistance, Mycelial Growth and Pathogenicity towards Wheat." International Journal of Molecular Sciences 19, no. 8: 2351.