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Prof. Marta López-Alonso
Departamento de Patoloxía Animal, Universidade de Santiago de Compostela, Facultad de Veterinaria. Campus Terra, Lugo, Spain

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0 Cattle
0 Organic Farming
0 Conventional Farming
0 Toxic and trace elements
0 Cattle medicine

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Cattle
Toxic and trace elements
Organic Farming
Conventional Farming

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Journal article
Published: 04 April 2021 in Marine Drugs
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The main objective was to determine the chemical, phytochemical, fatty acid and mineral profiles of three commercially relevant brown macroalgae (Laminaria digitata, Laminaria hyperborea and Ascophyllum nodosum) collected each season for two years off the west coast of Ireland. All the chemical, phytochemical, fatty acid and minerals analysed varied significantly depending on the macroalgal species, season and year of collection. Overall, the protein contents of macroalgae were negatively correlated with carbohydrate content. Protein (2–11%) was at its highest during winter and/or spring, decreasing to a minimum during summer and/or autumn. The three macroalgal species analysed in this study had clearly differentiated fatty acid profiles. The concentration of fatty acids was higher in A. nodosum compared with both Laminaria species. The mineral profile of the three macroalgal species was rich in essential metals, particularly Ca, Mg and P, while the levels of I were approximately 9- to 10-fold higher in both Laminaria spp. compared with A. nodosum. The levels of toxic metals (Cd, Hg and Pb) in all the macroalgal species studied were low in the current study; while the levels of total As were high (49–64 mg/kg DW macroalgae) compared with previous reports.

ACS Style

Marco Garcia-Vaquero; Gaurav Rajauria; Marta Miranda; Torres Sweeney; Marta Lopez-Alonso; John O’Doherty. Seasonal Variation of the Proximate Composition, Mineral Content, Fatty Acid Profiles and Other Phytochemical Constituents of Selected Brown Macroalgae. Marine Drugs 2021, 19, 204 .

AMA Style

Marco Garcia-Vaquero, Gaurav Rajauria, Marta Miranda, Torres Sweeney, Marta Lopez-Alonso, John O’Doherty. Seasonal Variation of the Proximate Composition, Mineral Content, Fatty Acid Profiles and Other Phytochemical Constituents of Selected Brown Macroalgae. Marine Drugs. 2021; 19 (4):204.

Chicago/Turabian Style

Marco Garcia-Vaquero; Gaurav Rajauria; Marta Miranda; Torres Sweeney; Marta Lopez-Alonso; John O’Doherty. 2021. "Seasonal Variation of the Proximate Composition, Mineral Content, Fatty Acid Profiles and Other Phytochemical Constituents of Selected Brown Macroalgae." Marine Drugs 19, no. 4: 204.

Journal article
Published: 31 December 2020 in Animals
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There is a notable lack of reference values for the renal resistive indices in the bovine kidney. Ultrasound (US) Doppler evaluation of these indices is a powerful, non-invasive technique for assessing, monitoring and diagnosing renal diseases in humans and other animals (e.g., small animals and horses). The aims of the present study were to establish a protocol for renal Doppler US in adult healthy Holstein-Friesian cows and to provide reference values for the renal resistive index (RI) and pulsatility index (PI). In cattle, the right kidney is always visible through a right abdominal window. Nevertheless, the left kidney is rarely accessible by transabdominal ultrasound. Doppler evaluation of the kidneys via a transabdominal approach is possible when accessible, but measurements can only be made in the larger vessels at the renal hilum. Normal RI and PI values were respectively 0.49 ± 0.07 and 0.70 ± 0.15 for the right kidney and 0.53 ± 0.05 and 0.79 ± 0.11 for the left kidney. We suggest an upper cut-off value for the RI of 0.63 and for the PI of 1.00 in healthy Holstein-Friesian cows. This is the first report describing normal values for the renal RI and PI in cattle that may be useful in future studies for characterizing different bovine pathologies that affect the renal parenchyma.

ACS Style

J. Daniel Barreiro-Vázquez; Marta Miranda; Andrés Barreiro-Lois. Transabdominal Renal Doppler Ultrasound in Healthy Adult Holstein-Friesian Cows: A Pilot Study. Animals 2020, 11, 63 .

AMA Style

J. Daniel Barreiro-Vázquez, Marta Miranda, Andrés Barreiro-Lois. Transabdominal Renal Doppler Ultrasound in Healthy Adult Holstein-Friesian Cows: A Pilot Study. Animals. 2020; 11 (1):63.

Chicago/Turabian Style

J. Daniel Barreiro-Vázquez; Marta Miranda; Andrés Barreiro-Lois. 2020. "Transabdominal Renal Doppler Ultrasound in Healthy Adult Holstein-Friesian Cows: A Pilot Study." Animals 11, no. 1: 63.

Journal article
Published: 07 December 2020 in Animals
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The objective of this study was to obtain information about the role of trace element imbalance in the pathogenesis of certain diseases in dogs and to evaluate the suitability of trace element profiling as an additional tool in the diagnosis. Serum trace element concentrations (copper, molybdenum, selenium and zinc) were measured in a cohort of healthy (control) dogs (n = 42) and dogs affected by hepatic (n = 25), gastrointestinal (n = 24), inflammatory/infection (n = 24), and renal (n = 22) diseases. These data were analyzed together with data on basic biochemical parameters (alanine aminotransferase, alkaline phosphatase, blood urea nitrogen, creatinine, albumin, globulin, and glucose) by using chemometric techniques. The chemometric analysis revealed distinctive association patterns between trace elements and biochemical parameters for each clinical disorders. The findings provide clear evidence for the important role of trace elements in disease, particularly in relation to acute phase reactions, with serum copper providing an indirect measurement of ceruloplasmin (positive acute-phase protein) and serum selenium and zinc acting as negative acute phase reactants. Molybdenum may also be a suitable marker of incipient renal disease. Thus, the analysis of trace element profiles, by multielement techniques, in a single serum sample would be a valuable additional tool for the diagnosis of certain diseases.

ACS Style

Yolanda Cedeño; Marta Miranda; Inmaculada Orjales; Carlos Herrero-Latorre; Maruska Suárez; Diego Luna; Marta López-Alonso. Trace Element Levels in Serum Are Potentially Valuable Diagnostic Markers in Dogs. Animals 2020, 10, 2316 .

AMA Style

Yolanda Cedeño, Marta Miranda, Inmaculada Orjales, Carlos Herrero-Latorre, Maruska Suárez, Diego Luna, Marta López-Alonso. Trace Element Levels in Serum Are Potentially Valuable Diagnostic Markers in Dogs. Animals. 2020; 10 (12):2316.

Chicago/Turabian Style

Yolanda Cedeño; Marta Miranda; Inmaculada Orjales; Carlos Herrero-Latorre; Maruska Suárez; Diego Luna; Marta López-Alonso. 2020. "Trace Element Levels in Serum Are Potentially Valuable Diagnostic Markers in Dogs." Animals 10, no. 12: 2316.

Review
Published: 15 October 2020 in Animals
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Ensuring adequate copper supplementation in ruminants is a challenging task due to the complexity of copper metabolism in these animals. The three-way interaction between copper, molybdenum and sulphur (Cu-Mo-S) in the rumen makes ruminants, particularly cattle, very susceptible to suffering from secondary copper deficiency. Paradoxically, excessive copper storage in the liver to prevent deficiency becomes a hazard when ruminants are fed copper-supplemented diets even slightly above requirements. While cattle were traditionally thought to be relatively tolerant of copper accumulation, and reports of copper poisoning were until recently somewhat rare, in recent years an increased number of episodes/outbreaks of copper toxicity in cattle, particularly in dairy cattle, have been reported worldwide. The growing number of lethal cases reported seems to indicate that copper intoxication is spreading silently in dairy herds, urging the development of strategies to monitor herd copper status and improve farmers’ awareness of copper toxicity. In fact, monitoring studies carried out on numerous samples collected from culled animals in slaughterhouses and/or diagnostic laboratories have demonstrated that large numbers of animals have hepatic copper concentrations well above adequate levels in many different countries. These trends are undoubtedly due to copper supplementation aimed at preventing copper deficiency, as dietary copper intake from pasture alone is unlikely to cause such high levels of accumulation in liver tissue. The reasons behind the copper overfeeding in cattle are related both to a poor understanding of copper metabolism and the theory of “if adding a little produces a response, then adding a lot will produce a better response”. Contrary to most trace elements, copper in ruminants has narrow margins of safety, which must also be formulated considering the concentrations of copper antagonists in the diet. This review paper aims to provide nutritionists/veterinary practitioners with the key points about copper metabolism in cattle to guarantee an adequate copper supply while preventing excessive hepatic copper loading, which requires à la carte copper supplementation for each herd.

ACS Style

Marta López-Alonso; Marta Miranda. Copper Supplementation, A Challenge in Cattle. Animals 2020, 10, 1890 .

AMA Style

Marta López-Alonso, Marta Miranda. Copper Supplementation, A Challenge in Cattle. Animals. 2020; 10 (10):1890.

Chicago/Turabian Style

Marta López-Alonso; Marta Miranda. 2020. "Copper Supplementation, A Challenge in Cattle." Animals 10, no. 10: 1890.

Journal article
Published: 10 July 2020 in Animals
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Ultrasonographic reference values for the adrenal glands of cattle have not been reported to date. Adrenal glands can be affected by different pathologies, such as hyperplasia, neoplasia and atrophy (either primary or secondary). The present findings indicate that the right adrenal gland can be easily characterized by transabdominal ultrasound in adult Holstein–Friesian cows, with no apparent influence of age or weight. The right adrenal gland (mean length 3.86 ± 1.39 cm; and mean thickness 1.39 ± 0.26 cm) was consistently and mainly located in the 12th intercostal space. The left adrenal gland was more difficult to locate due to its more medial position, and to the presence of gas in the gastrointestinal tract, so it could not be visualized in most animals (18/25). Its mean length was 3.72 ± 0.95 cm, and mean thickness was 1.36 ± 0.33 cm, in the sagittal section. This is the first report of the ultrasonographic appearance of the adrenal glands of cows and of the corresponding reference preliminary values.

ACS Style

J. Daniel Barreiro-Vázquez; Andrés Barreiro-Lois; Marta Miranda. Ultrasonography of Normal Adrenal Glands in Adult Holstein–Friesian Cows: A Pilot Study. Animals 2020, 10, 1171 .

AMA Style

J. Daniel Barreiro-Vázquez, Andrés Barreiro-Lois, Marta Miranda. Ultrasonography of Normal Adrenal Glands in Adult Holstein–Friesian Cows: A Pilot Study. Animals. 2020; 10 (7):1171.

Chicago/Turabian Style

J. Daniel Barreiro-Vázquez; Andrés Barreiro-Lois; Marta Miranda. 2020. "Ultrasonography of Normal Adrenal Glands in Adult Holstein–Friesian Cows: A Pilot Study." Animals 10, no. 7: 1171.

Journal article
Published: 18 June 2020 in Animals
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This study was designed (i) to establish reference ranges for the essential trace element and background levels of toxic element exposure in the healthy/normal dog population, and (ii) to evaluate whether trace element concentrations vary in dogs suffering from different pathologies. Blood serum samples were collected from 187 healthy and diseased dogs at the Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Santiago de Compostela (northwest Spain). The samples were acid digested, and the concentrations of trace elements (Co, Cr, Cu, Fe, Mn, Mo, Ni, Se and Zn) and toxic elements (As, Cd, Hg and Pb) were determined by inductively coupled plasma-mass spectrometry (ICP-MS). This enabled us to establish reference ranges for the essential trace elements and the level of toxic element exposure in dogs, and to identify several clinical situations associated with variations in trace elements in serum. Relative to concentrations in healthy control dogs, statistically significant differences were observed in the concentrations of Cu (significantly higher in hepatic, inflammatory/infectious and oncological categories), Mo (significantly higher in renal category), Se (significantly lower in gastrointestinal category) and Zn (significantly lower in gastrointestinal, inflammatory/infectious and renal categories). Trace element concentrations can be a cause or consequence of disease, and the study findings indicate that trace element determination in serum provides useful information on the pathogenesis of certain diseases. Further research on the serum concentrations of trace elements, particularly in relation to other biochemical parameters and diagnostic tools, may provide valuable information for the diagnosis of diseases in dogs and the disease prognosis.

ACS Style

Yolanda Cedeño; Marta Miranda; Inmaculada Orjales; Carlos Herrero-Latorre; Maruska Suárez; Diego Luna; Marta López-Alonso. Serum Concentrations of Essential Trace and Toxic Elements in Healthy and Disease-Affected Dogs. Animals 2020, 10, 1052 .

AMA Style

Yolanda Cedeño, Marta Miranda, Inmaculada Orjales, Carlos Herrero-Latorre, Maruska Suárez, Diego Luna, Marta López-Alonso. Serum Concentrations of Essential Trace and Toxic Elements in Healthy and Disease-Affected Dogs. Animals. 2020; 10 (6):1052.

Chicago/Turabian Style

Yolanda Cedeño; Marta Miranda; Inmaculada Orjales; Carlos Herrero-Latorre; Maruska Suárez; Diego Luna; Marta López-Alonso. 2020. "Serum Concentrations of Essential Trace and Toxic Elements in Healthy and Disease-Affected Dogs." Animals 10, no. 6: 1052.

Journal article
Published: 30 April 2020 in Science of The Total Environment
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The Lower Amazon region (Western Pará, northern Brazil) is greatly affected by mining exploitations (particularly artisanal gold mines) and other industrial and intensive agricultural activities with potentially strong impacts on aquatic ecosystems. Although such impacts include contamination with various toxic elements, to date only the effects of Hg have been considered. In this study, toxic and trace element concentrations were determined in the flesh of 351 fish specimens, including detritivores (Acarí, Pterygoplichthys pardalis), omnivores (Piranha, Pygocentrus nattereri; Pirarucu, Arapaima sp.) and carnivores (Caparari, Pseudoplatystoma fasciatum; Tucunaré, Cichla ocellaris), during the dry and wet seasons in 2015 and 2016. The range of concentrations of toxic element residues were 2–238 μg/kg fresh weight for As, 1–77 μg/kg for Cd, 4–1922 μg/kg for Hg and 1–30 μg/kg for Pb. Only the maximum concentrations of Hg established in the Brazilian legislation for fish destined for human consumption (0.5 mg/kg) were exceeded (in 16% of carnivorous species). The large between-species and seasonal differences observed for all these toxic elements are probably related to the seasonal behaviour and dietary habits of the different fish species. By contrast, essential trace element concentrations were low and not related to seasonal or dietary factors, and the observed differences may be at least partly related to the metabolism of each species. The associations between Hg and the essential trace elements Se, Fe, Co and Mn deserve special attention, as these trace elements may play a role in Hg cycling and methylation and merit further evaluation with the aim of reducing Hg toxicity in aquatic environments.

ACS Style

Fabio Edir Amaral Albuquerque; Antonio Humberto Hamad Minervino; Marta Miranda; Carlos Herrero-Latorre; Raimundo Alves Barrêto Júnior; Francisco Leonardo Costa Oliveira; Maria Claudia Araripe Sucupira; Enrico Lippi Ortolani; Marta López-Alonso. Toxic and essential trace element concentrations in fish species in the Lower Amazon, Brazil. Science of The Total Environment 2020, 732, 138983 .

AMA Style

Fabio Edir Amaral Albuquerque, Antonio Humberto Hamad Minervino, Marta Miranda, Carlos Herrero-Latorre, Raimundo Alves Barrêto Júnior, Francisco Leonardo Costa Oliveira, Maria Claudia Araripe Sucupira, Enrico Lippi Ortolani, Marta López-Alonso. Toxic and essential trace element concentrations in fish species in the Lower Amazon, Brazil. Science of The Total Environment. 2020; 732 ():138983.

Chicago/Turabian Style

Fabio Edir Amaral Albuquerque; Antonio Humberto Hamad Minervino; Marta Miranda; Carlos Herrero-Latorre; Raimundo Alves Barrêto Júnior; Francisco Leonardo Costa Oliveira; Maria Claudia Araripe Sucupira; Enrico Lippi Ortolani; Marta López-Alonso. 2020. "Toxic and essential trace element concentrations in fish species in the Lower Amazon, Brazil." Science of The Total Environment 732, no. : 138983.

Original article
Published: 23 December 2019 in International Journal of Consumer Studies
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Although the organic sector is still relatively small, the demand for organic food is increasing throughout the world. The characterization of consumers' perception of and attitudes towards organic food is important to enable the development of marketing policies aimed at attracting conventional consumers to the sector. Consumers' behaviour studies must be conducted specifically for different regions and countries as perceptions and attitudes vary across the world. In the present study, a questionnaire was designed for administration to consumers in Galicia (Northern Spain). The questionnaire was administered to 830 consumers in 200 establishments to obtain data about the consumers' perception of and attitudes towards organic food. The survey results showed that one‐third of responders consume organic food and that the typical profile of a consumer of organic food is a middle‐aged, medium‐high class, university‐educated female living in a large village, who shops in supermarkets and preferably consume vegetables, fruits and eggs. Most people who declared that they consume organic products confuse these with home and locally produced food, indicating the potential for growth of the organic sector by providing such consumers with appropriate information. Most consumers (including conventional consumers) have a good opinion of organic food and consider that it is better for health, is of better quality than conventional food and avoid pesticide residues. However, price continues to be a barrier to the consumption of organic produce. Most respondents stated that they would consume more organic food if the price was only between 10% and 30% higher than the conventional equivalent. Finally, organic consumers in Galicia showed positive attitudes towards using local breeds in organic agriculture, both for producing food and for ecotourism and educational activities. Such activities could contribute to conserving breed biodiversity and adding value to organic farming.

ACS Style

Ruth Rodríguez‐Bermúdez; Marta Miranda; Inmaculada Orjales; María José Ginzo‐Villamayor; Wajih Al‐Soufi; Marta López‐Alonso. Consumers' perception of and attitudes towards organic food in Galicia (Northern Spain). International Journal of Consumer Studies 2019, 44, 206 -219.

AMA Style

Ruth Rodríguez‐Bermúdez, Marta Miranda, Inmaculada Orjales, María José Ginzo‐Villamayor, Wajih Al‐Soufi, Marta López‐Alonso. Consumers' perception of and attitudes towards organic food in Galicia (Northern Spain). International Journal of Consumer Studies. 2019; 44 (3):206-219.

Chicago/Turabian Style

Ruth Rodríguez‐Bermúdez; Marta Miranda; Inmaculada Orjales; María José Ginzo‐Villamayor; Wajih Al‐Soufi; Marta López‐Alonso. 2019. "Consumers' perception of and attitudes towards organic food in Galicia (Northern Spain)." International Journal of Consumer Studies 44, no. 3: 206-219.

Original article
Published: 25 November 2019 in Reproduction in Domestic Animals
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Organic farm management combines best environmental practices, a high level of biodiversity, preservation of natural resources and high animal welfare standards. To meet these criteria, farmers must have livestock well adapted to local organic conditions and information about how different breeds and crosses perform under different conditions. The objective of this study was to compare the performance of different pure breeds and cross‐breeds of cattle in organic dairy systems in Northern Spain. The data analysed were obtained from monthly records kept between 2010 and 2016 on organic farms registered in the regional Milk Recording System. Analysis of various traits indicated that the Holstein‐Friesian breed suits the organic production system in the study region. Although the reproductive performance of Holstein‐Friesian cows was poorer (in terms of number of services per conception) than that of cross‐breed and Brown Swiss cows, the Holstein‐Friesian produced more milk and lived longer. In addition, there was no difference in calving type or calving ease between the different breed groups. The better milk fat and protein yields produced by the crosses may be useful traits for farmers interested in milk transformation. The advantage of continuing to use Holstein‐Friesian cattle is that the breed is predominant worldwide and the genealogy is well documented. If Holstein‐Friesian cattle continue to be used, the main priority will be to search for well‐adapted bulls (particularly for pasture‐based conditions) and to elaborate a genetic merit index for organic and pasture‐based systems with the aim of predicting and minimizing genotype x environment interactions.

ACS Style

Ruth Rodríguez‐Bermúdez; Marta Miranda; Ramiro Fouz; Inmaculada Orjales; Francisco Javier Diéguez; Antonio Humberto Hamad Minervino; Marta López‐Alonso. Breed performance in organic dairy farming in Northern Spain. Reproduction in Domestic Animals 2019, 55, 93 -104.

AMA Style

Ruth Rodríguez‐Bermúdez, Marta Miranda, Ramiro Fouz, Inmaculada Orjales, Francisco Javier Diéguez, Antonio Humberto Hamad Minervino, Marta López‐Alonso. Breed performance in organic dairy farming in Northern Spain. Reproduction in Domestic Animals. 2019; 55 (1):93-104.

Chicago/Turabian Style

Ruth Rodríguez‐Bermúdez; Marta Miranda; Ramiro Fouz; Inmaculada Orjales; Francisco Javier Diéguez; Antonio Humberto Hamad Minervino; Marta López‐Alonso. 2019. "Breed performance in organic dairy farming in Northern Spain." Reproduction in Domestic Animals 55, no. 1: 93-104.

Research article
Published: 19 November 2019 in Environmental Science and Pollution Research
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Recycling sewage sludge by applying it to agricultural land is strategically important in the European Union and is regulated by Directive 86/278/EEC, aimed at protecting the soil and humans from the presence of unwanted substances. However, because of the ruminant feeding habits, there is a risk that animals grazed on pasture or fed crops grown on land treated in this way may ingest biosolids adhered to foliage and/or on the top soil. This paper describes an episode of toxicity in a dairy herd consuming silage from a field fertilized with sewage sludge produced in a wastewater treatment plant. The affected cows were recumbent, unable to rise and suffered diarrhoea. Analysis of tissues (fresh weight) from a cow that subsequently died revealed severe hepatic iron loading (6720 mg/kg) and secondary multi-trace element hepatic deficiency, particularly of copper (0.812 mg/kg) and manganese (0.436 mg/kg), but also selenium (0.164 mg/kg) and zinc (19.9 mg/kg). The study findings indicate that the use of sewage sludge in agriculture can cause secondary multi-trace element deficiencies in ruminants. Careful attention should be taken when crops are ensilaged avoiding top-soil sewage sludge contamination, since the acidification process may greatly increase Fe bioavailability.

ACS Style

Marta Miranda; Luisa Méndez; Víctor Pereira; Antonio Humberto Hamad Minervino; Marta López-Alonso. Iron loading and secondary multi-trace element deficiency in a dairy herd fed silage grass grown on land fertilized with sewage sludge. Environmental Science and Pollution Research 2019, 26, 36978 -36984.

AMA Style

Marta Miranda, Luisa Méndez, Víctor Pereira, Antonio Humberto Hamad Minervino, Marta López-Alonso. Iron loading and secondary multi-trace element deficiency in a dairy herd fed silage grass grown on land fertilized with sewage sludge. Environmental Science and Pollution Research. 2019; 26 (36):36978-36984.

Chicago/Turabian Style

Marta Miranda; Luisa Méndez; Víctor Pereira; Antonio Humberto Hamad Minervino; Marta López-Alonso. 2019. "Iron loading and secondary multi-trace element deficiency in a dairy herd fed silage grass grown on land fertilized with sewage sludge." Environmental Science and Pollution Research 26, no. 36: 36978-36984.

Journal article
Published: 05 November 2019 in Journal of Food Composition and Analysis
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This study aimed to evaluate the concentrations of toxic elements (As, Cd, Hg, Pb) and essential trace elements (Co, Cu, Cr, Fe, Mn, Mo, Ni, Se, Zn) in the freshwater shrimp M. amazonicum in the region of Santarem (lower Amazonia), which is affected by mining activities. The level of metal accumulation in the shrimp was found to be similar to that measured in other regions affected by anthropogenic polluted in both in Brazil and elsewhere, and does not pose a significant risk for human consumption. The different patterns of metal accumulation between the Amazon and Tapajos basins appear to be (at least partly) related to respectively bauxite exploitation and gold mining in the region. Bioaccumulation of the trace elements Co, Fe, and Mn mainly occurred in the visceral part of the shrimps from the Amazon river.

ACS Style

Fabio Edir Amaral Albuquerque; Antonio Humberto Hamad Minervino; Marta Miranda; Carlos Herrero-Latorre; Raimundo Alves Barrêto Júnior; Francisco Leonardo Costa Oliveira; Salatiel Ribeiro Dias; Enrico Lippi Ortolani; Marta López-Alonso. Toxic and essential trace element concentrations in the freshwater shrimp Macrobrachium amazonicum in the Lower Amazon, Brazil. Journal of Food Composition and Analysis 2019, 86, 103361 .

AMA Style

Fabio Edir Amaral Albuquerque, Antonio Humberto Hamad Minervino, Marta Miranda, Carlos Herrero-Latorre, Raimundo Alves Barrêto Júnior, Francisco Leonardo Costa Oliveira, Salatiel Ribeiro Dias, Enrico Lippi Ortolani, Marta López-Alonso. Toxic and essential trace element concentrations in the freshwater shrimp Macrobrachium amazonicum in the Lower Amazon, Brazil. Journal of Food Composition and Analysis. 2019; 86 ():103361.

Chicago/Turabian Style

Fabio Edir Amaral Albuquerque; Antonio Humberto Hamad Minervino; Marta Miranda; Carlos Herrero-Latorre; Raimundo Alves Barrêto Júnior; Francisco Leonardo Costa Oliveira; Salatiel Ribeiro Dias; Enrico Lippi Ortolani; Marta López-Alonso. 2019. "Toxic and essential trace element concentrations in the freshwater shrimp Macrobrachium amazonicum in the Lower Amazon, Brazil." Journal of Food Composition and Analysis 86, no. : 103361.

Journal article
Published: 21 July 2019 in Animals
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This study was designed to evaluate the influence of type of blood sample (serum or plasma) on essential and toxic element analysis in cattle. Paired plasma and serum samples (n = 20) were acid digested, and the concentrations of As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, Li, Mg, Mn. Mo, Ni, P, Pb, Sb, Se, Sr and Zn were determined by inductively coupled plasma mass spectrometry (ICP-MS). The study findings indicate that plasma and serum samples appear suitable and interchangeable for the determination of most of the essential and toxic elements in blood in cattle. The only exceptions are Cu and Se, the concentrations of which were significantly lower (40.9 and 29.9% respectively) in serum than in plasma. Some of the Cu in blood samples from bovine ruminants is known to be sequestered during clotting. However, further research on Se in ruminants and other animal species is warranted. Finally, the significantly higher Mn (9.9%) concentrations in serum than in plasma may have been caused by haemolysis of some samples. Special attention should be paid to preventing haemolysis of samples during collection and processing, in order to prevent overestimation of elements present at high concentrations inside erythrocytes (i.e., Fe, Mn and Zn).

ACS Style

Diego Luna; Marta López-Alonso; Yolanda Cedeño; Lucas Rigueira; Víctor Pereira; Marta Miranda. Determination of Essential and Toxic Elements in Cattle Blood: Serum vs Plasma. Animals 2019, 9, 465 .

AMA Style

Diego Luna, Marta López-Alonso, Yolanda Cedeño, Lucas Rigueira, Víctor Pereira, Marta Miranda. Determination of Essential and Toxic Elements in Cattle Blood: Serum vs Plasma. Animals. 2019; 9 (7):465.

Chicago/Turabian Style

Diego Luna; Marta López-Alonso; Yolanda Cedeño; Lucas Rigueira; Víctor Pereira; Marta Miranda. 2019. "Determination of Essential and Toxic Elements in Cattle Blood: Serum vs Plasma." Animals 9, no. 7: 465.

Journal article
Published: 01 July 2019 in Revista Agraria Academica
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ACS Style

Emanuel Felipe De Oliveira Filho; Pierre Castro Soares; Marta Miranda; José Augusto Bastos Afonso; Carla Lopes Mendonça; Rodolfo José Cavalcanti Souto; Daniel Nunes De Araújo Gonçalves; Antônio Flávio Medeiros Dantas; Taciana Holanda Kunst; Ana Paula Silveira Paim; Marta López-Alonso. Outbreak of enzootic ataxia in lambs in the semi-arid state of Pernambuco, Brazil. Revista Agraria Academica 2019, 2, 1 .

AMA Style

Emanuel Felipe De Oliveira Filho, Pierre Castro Soares, Marta Miranda, José Augusto Bastos Afonso, Carla Lopes Mendonça, Rodolfo José Cavalcanti Souto, Daniel Nunes De Araújo Gonçalves, Antônio Flávio Medeiros Dantas, Taciana Holanda Kunst, Ana Paula Silveira Paim, Marta López-Alonso. Outbreak of enzootic ataxia in lambs in the semi-arid state of Pernambuco, Brazil. Revista Agraria Academica. 2019; 2 (4):1.

Chicago/Turabian Style

Emanuel Felipe De Oliveira Filho; Pierre Castro Soares; Marta Miranda; José Augusto Bastos Afonso; Carla Lopes Mendonça; Rodolfo José Cavalcanti Souto; Daniel Nunes De Araújo Gonçalves; Antônio Flávio Medeiros Dantas; Taciana Holanda Kunst; Ana Paula Silveira Paim; Marta López-Alonso. 2019. "Outbreak of enzootic ataxia in lambs in the semi-arid state of Pernambuco, Brazil." Revista Agraria Academica 2, no. 4: 1.

Journal article
Published: 22 June 2019 in Animals
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In the past, hepatic blood flow in cows was invasively characterized to investigate different pathologies and physiological conditions. However, hepatic blood flow can be easily evaluated with transabdominal Doppler ultrasound. Sixteen healthy adult non-lactating, non-pregnant Holstein-Friesian cows were examined using B-mode and Doppler ultrasound between the right flank and 9th intercostal space to establish the best approach to the different parts of the portal and hepatic vein systems, and determine normal blood flow characteristics. The main portal vein was characterized by a turbulent, high-velocity flow due to the opposing confluence of the splenic and cranial mesenteric veins, while hepatic and caudal vena cava veins have laminar blood flow, in which the phasicity is considered mainly respiratory in origin. Reference values were determined in relation to the anatomical point of observation. In conclusion, transabdominal Doppler ultrasound of the portal system is a simple technique that allows non-invasive characterization of portal and hepatic blood haemodynamics in cows.

ACS Style

J. Daniel Barreiro-Vázquez; Marta Miranda; M. Isabel Barreiro-Vilanova; F. Javier Diéguez; Andrés Barreiro-Lois. Characterization of the Normal Portal and Hepatic Blood Flow of Adult Holstein-Friesian Cows. Animals 2019, 9, 386 .

AMA Style

J. Daniel Barreiro-Vázquez, Marta Miranda, M. Isabel Barreiro-Vilanova, F. Javier Diéguez, Andrés Barreiro-Lois. Characterization of the Normal Portal and Hepatic Blood Flow of Adult Holstein-Friesian Cows. Animals. 2019; 9 (6):386.

Chicago/Turabian Style

J. Daniel Barreiro-Vázquez; Marta Miranda; M. Isabel Barreiro-Vilanova; F. Javier Diéguez; Andrés Barreiro-Lois. 2019. "Characterization of the Normal Portal and Hepatic Blood Flow of Adult Holstein-Friesian Cows." Animals 9, no. 6: 386.

Comparative study
Published: 17 April 2019 in Reproduction in Domestic Animals
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Organic farming has traditionally given preference to indigenous breeds that are well adapted to local conditions; however, current trends towards increased yields may produce less robust animals with potentially low fertility. The Holstein-Friesian breed dominates dairy sector, although there is a general concern that these cows may not be well adapted to organic systems. This study aims to evaluate the reproductive performance of organic dairy herds in northern Spain, by comparing organically reared Holstein-Friesian with their counterparts reared on conventional farms and also with other organically reared breeds and crosses. Reproductive parameters were obtained for individual cows on organic, conventional pasture-based and conventional zero-grazing farms. The reproductive performance of Holstein-Friesian cows was slightly better in organic systems than in pasture-based conventional systems, but not than in zero-grazing conventional systems. The comparison of reproductive performance of Holstein-Friesian from organic and conventional systems and with other organically reared breeds and crosses enabled us to demonstrate that it is not the organic system that constrains the reproductive performance of Holstein-Friesian, but that the manifestation of oestrus is less marked than in other breeds. Breeding selection considering reproductive traits and appropriate management of oestrous detection would improve the reproductive performance of Holstein-Friesian cows.

ACS Style

Ruth Rodríguez‐Bermúdez; Ramiro Fouz; Marta Miranda; Inmaculada Orjales; Antonio Humberto Hamad Minervino; Marta López‐Alonso. Organic or conventional dairy farming in northern Spain: Impacts on cow reproductive performance. Reproduction in Domestic Animals 2019, 54, 902 -911.

AMA Style

Ruth Rodríguez‐Bermúdez, Ramiro Fouz, Marta Miranda, Inmaculada Orjales, Antonio Humberto Hamad Minervino, Marta López‐Alonso. Organic or conventional dairy farming in northern Spain: Impacts on cow reproductive performance. Reproduction in Domestic Animals. 2019; 54 (6):902-911.

Chicago/Turabian Style

Ruth Rodríguez‐Bermúdez; Ramiro Fouz; Marta Miranda; Inmaculada Orjales; Antonio Humberto Hamad Minervino; Marta López‐Alonso. 2019. "Organic or conventional dairy farming in northern Spain: Impacts on cow reproductive performance." Reproduction in Domestic Animals 54, no. 6: 902-911.

Research article
Published: 05 February 2019 in PLoS ONE
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Here we propose a single acid digestion (SAD) sample preparation method for ICP-MS analysis of animal serum samples to determine trace element contents. The method was evaluated in comparison with a commonly used procedure involving dilution of samples in an alkaline solution (AKD). In the SAD procedure, aliquots (1 mL) of bovine serum samples were treated at low temperature with a mixture of concentrated nitric acid and hydrogen peroxide. Trace elements (As, B, Ba, Cd, Co, Cr, Cu, Fe, Hg, Li, Mn, Mo, Ni, Pb, Sb, Se, Sr, U, and Zn) were directly determined by ICP-MS analysis of diluted solutions of samples. Both methods were sufficiently sensitive to enable quantification of most trace elements, with the exception of the AKD method for Cd, Hg and Pb. The quality of the data was verified by using certified reference material. Good results were obtained for the SAD procedure and all elements, but recoveries were unacceptable with the AKD procedure for Se (recovery: 57%), Cd (154%) and Fe (139%). Strong associations (R2>0.90, P = 0.000) between the data obtained by both methods were demonstrated for the elements considered. The proposed SAD sample preparation method produced satisfactory results for determining most toxic and essential trace elements targeted in monitoring studies.

ACS Style

Diego Luna; Marta Miranda; Antonio Humberto Hamad Minervino; Verónica Piñeiro; Carlos Herrero Latorre; Marta López-Alonso. Validation of a simple sample preparation method for multielement analysis of bovine serum. PLoS ONE 2019, 14, e0211859 .

AMA Style

Diego Luna, Marta Miranda, Antonio Humberto Hamad Minervino, Verónica Piñeiro, Carlos Herrero Latorre, Marta López-Alonso. Validation of a simple sample preparation method for multielement analysis of bovine serum. PLoS ONE. 2019; 14 (2):e0211859.

Chicago/Turabian Style

Diego Luna; Marta Miranda; Antonio Humberto Hamad Minervino; Verónica Piñeiro; Carlos Herrero Latorre; Marta López-Alonso. 2019. "Validation of a simple sample preparation method for multielement analysis of bovine serum." PLoS ONE 14, no. 2: e0211859.

Review
Published: 01 February 2019 in Journal of Dairy Research
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Organic farming is believed by many to be an environmentally friendly production system that promotes the use of local forage while strongly limiting the input of chemicals, including allopathic treatments. As organic dairy farming has grown, farmers have realised that many available conventional breeds of cow are not well adapted to the new situations and that more ‘robust’ cows, able to function well in the constraining organic environment, are needed to yield acceptable longevity and productivity. In this review paper, the current breed diversity in organic dairy farming is analysed with the aim of identifying the types of cow that would best fulfil organic breeding goals. Unlike the conventional sector, organic dairy farming is very heterogeneous and no single type of cow can adapt well to all scenarios. There are advantages and disadvantages to the use of existing breeds (rustic Holstein-Friesian, other rustic breeds and crosses), and strong genotype × environment interactions demand different strategies for very diverse situations. Organic dairy farms producing milk for systems that recompense milk volume would benefit from using higher milk yielding cows, and rustic Holstein-Friesian cows may be the best option in such cases. Although most Holstein-Friesian cows are currently selected for use in conventional systems, this situation could be reversed by the implementation of an organic merit index that includes organic breeding goals. Farms producing milk either for systems that recompense milk solids or for transformation into dairy products would benefit from using breeds other than Holstein-Friesian or their crosses. Organic farmers who focus on rural tourism, farm schools or other businesses in which marketing strategies must be taken into account could benefit from using local breeds (when possible) or other rustic breeds that are highly valued by consumers.

ACS Style

Ruth Rodríguez-Bermúdez; Marta Miranda; Javier Baudracco; Ramiro Fouz; Víctor Pereira; Marta López-Alonso. Breeding for organic dairy farming: what types of cows are needed? Journal of Dairy Research 2019, 86, 3 -12.

AMA Style

Ruth Rodríguez-Bermúdez, Marta Miranda, Javier Baudracco, Ramiro Fouz, Víctor Pereira, Marta López-Alonso. Breeding for organic dairy farming: what types of cows are needed? Journal of Dairy Research. 2019; 86 (1):3-12.

Chicago/Turabian Style

Ruth Rodríguez-Bermúdez; Marta Miranda; Javier Baudracco; Ramiro Fouz; Víctor Pereira; Marta López-Alonso. 2019. "Breeding for organic dairy farming: what types of cows are needed?" Journal of Dairy Research 86, no. 1: 3-12.

Comparative study
Published: 01 January 2019 in Animal
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The energy supplied by the high-forage diets used in organic farming may be insufficient to meet the requirements of dairy cattle. However, few studies have considered this problem. The present study aimed to analyze the composition of the diets and the nutritional status (focusing on the energy–protein balance of the diets) of dairy cattle reared on organic farms in northern Spain, which are similar to other organic farming systems in temperate regions. Exhaustive information about diets was obtained from organic (ORG) and representative conventional grazing (GRZ) and conventional no-grazing (CNG) farms. Samples of feed from the respective farms were analyzed to determine the composition. Overall, the diets used on the ORG farms were very different from those used on the CNG farms, although the difference was not as evident for GRZ. The CNG farms were characterized by a higher total dry matter intake with a high proportion of concentrate feed, maize silage and forage silage. By contrast, on ORG and GRZ farms, the forage, pasture and fibre intake were the most important variables. The ration used on ORG farms contained a significantly higher percentage of ADF and lower organic matter (OM) content than the rations used in both of the conventional farming systems, indicating that the diets in the former were less digestible. Although the protein concentration in the diets used on the grazing farms (ORG and GRZ) was higher than those used on CNG farms, the protein intake was similar. The results indicated an imbalance between energy and protein due to the low level of energy provided by the ORG diets, suggesting that more microbial protein could be synthesized from the available rumen-degraded dietary nitrogen if rumen-fermentable OM was not limiting. The imbalance between energy and protein led to a reduced amount of total digestible protein reaching the intestine and a lower milk yield per kilogram of CP intake on the ORG farms. In order to improve the protein use efficiency and consequently to reduce the loss of nitrogen to the environment, organic farming should aim to increase the energy content of cattle diets by improving forage quality and formulating rations with more balanced combinations of forage and grain.

ACS Style

I. Orjales; M. Lopez-Alonso; M. Miranda; Hector Alaiz Moreton; C. Resch; S. López. Dairy cow nutrition in organic farming systems. Comparison with the conventional system. Animal 2019, 13, 1084 -1093.

AMA Style

I. Orjales, M. Lopez-Alonso, M. Miranda, Hector Alaiz Moreton, C. Resch, S. López. Dairy cow nutrition in organic farming systems. Comparison with the conventional system. Animal. 2019; 13 (5):1084-1093.

Chicago/Turabian Style

I. Orjales; M. Lopez-Alonso; M. Miranda; Hector Alaiz Moreton; C. Resch; S. López. 2019. "Dairy cow nutrition in organic farming systems. Comparison with the conventional system." Animal 13, no. 5: 1084-1093.

Journal article
Published: 30 November 2018 in Animals
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The aim of this study was to evaluate whether zinc (Zn) supplementation protects against hepatic copper (Cu) accumulation in copper-loaded sheep. Forty cross-bred lambs were assigned to five experimental groups. These included the control group (C) and four treatment groups that received Cu and/or Zn supplementation (dry matter (DM) basis) over 14 weeks, as follows: Cu (450 mg Cu/kg); Zn-35 (450 mg Cu + 35 mg Zn/kg); Zn-150 (450 mg Cu + 150 mg Zn/kg); and Zn-300 (450 mg Cu + 300 mg Zn/kg). Blood, liver, and bile samples were obtained for mineral determination by inductively coupled plasma optical emission spectrometry (ICP–OES). The hepatic metallothionein (MT) concentrations were also determined. At the end of the experiment, hepatic Cu concentrations were higher in all Cu-supplemented groups than in C. Hepatic Cu accumulation was lower in the groups receiving the Zn supplementation than in the Cu group, although the difference was only statistically significant (66%) in the Zn-300 group. The MT concentrations tended to be higher (almost two-fold) in the Zn groups (but were not dose related) than in the C and Cu groups, and they were related to hepatic Zn concentrations. Zn supplementation at 300 mg/kg DM is useful for preventing excessive hepatic Cu accumulation in sheep exposed to high dietary concentrations of Cu.

ACS Style

Antonio Humberto Hamad Minervino; Marta López-Alonso; Raimundo Alves Barrêto Júnior; Frederico Augusto Mazzocca Lopes Rodrigues; Carolina Akiko Sato Cabral Araújo; Rejane Santos Sousa; Clara Satsuk Mori; Marta Miranda; Francisco Leonardo Costa Oliveira; Alexandre Coutinho Antonelli; Enrico Lippi Ortolani. Dietary Zinc Supplementation to Prevent Chronic Copper Poisoning in Sheep. Animals 2018, 8, 227 .

AMA Style

Antonio Humberto Hamad Minervino, Marta López-Alonso, Raimundo Alves Barrêto Júnior, Frederico Augusto Mazzocca Lopes Rodrigues, Carolina Akiko Sato Cabral Araújo, Rejane Santos Sousa, Clara Satsuk Mori, Marta Miranda, Francisco Leonardo Costa Oliveira, Alexandre Coutinho Antonelli, Enrico Lippi Ortolani. Dietary Zinc Supplementation to Prevent Chronic Copper Poisoning in Sheep. Animals. 2018; 8 (12):227.

Chicago/Turabian Style

Antonio Humberto Hamad Minervino; Marta López-Alonso; Raimundo Alves Barrêto Júnior; Frederico Augusto Mazzocca Lopes Rodrigues; Carolina Akiko Sato Cabral Araújo; Rejane Santos Sousa; Clara Satsuk Mori; Marta Miranda; Francisco Leonardo Costa Oliveira; Alexandre Coutinho Antonelli; Enrico Lippi Ortolani. 2018. "Dietary Zinc Supplementation to Prevent Chronic Copper Poisoning in Sheep." Animals 8, no. 12: 227.

Journal article
Published: 20 November 2018 in Journal of Trace Elements in Medicine and Biology
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The aim of the present study was to establish the pattern of lobular distribution of trace elements in the liver of cattle. The objective was to determine which part of the liver would provide accurate estimation of the trace element content of the whole organ. Liver samples were obtained from 10 Holstein-Friesian (HF), 10 Galician Blond (GB) and 10 GBxHF crosses (all aged 10 months) at slaughter. Samples were taken from 6 regions of the liver: the internal and external faces of the right lobe (IR and ER respetively); the left lobe (L), caudate lobe (CAU), quadrate lobe (QUA) and the processus papillaris (PP). The samples were acid digested and trace elements were determined by inductively coupled plasma mass spectrometry. The distribution of all trace elements, except cobalt and zinc, varied significant across the liver. In all cases, the concentrations were highest in L and lowest in CAU. Variations in the distribution between the other areas of the liver (ER, IR, QUA, PP) were not significant. The distribution of trace elements may be related to oxygen perfusion. Moreover, the trace element content of CAU was weakly correlated with those of the other lobes, and the capacity of L to accumulate high levels of trace elements would only be observed at high levels of exposure. Taking into account the main findings of the study, a single sample of liver taken from the same anatomical region (excluding CAU and L) would be adequate for determining the trace element status of cattle.

ACS Style

Diego Luna; Marta Miranda; Víctor Pereira; Marta López-Alonso. Variation in trace element content between liver lobes in cattle. How important is the sampling site? Journal of Trace Elements in Medicine and Biology 2018, 52, 53 -57.

AMA Style

Diego Luna, Marta Miranda, Víctor Pereira, Marta López-Alonso. Variation in trace element content between liver lobes in cattle. How important is the sampling site? Journal of Trace Elements in Medicine and Biology. 2018; 52 ():53-57.

Chicago/Turabian Style

Diego Luna; Marta Miranda; Víctor Pereira; Marta López-Alonso. 2018. "Variation in trace element content between liver lobes in cattle. How important is the sampling site?" Journal of Trace Elements in Medicine and Biology 52, no. : 53-57.