The dynamics of hydrocyclones is complex, because it is a multiphase flow problem that involves interaction between a discrete phase and multiple continuum phases. The performance of hydrocyclones is evaluated by using Computational Fluid Dynamics (CFD), and it is characterized by the pressure drop, split water ratio, and particle collection efficiency. In this paper, a computational model to improve and evaluate hydrocyclone performance is proposed. Four known computational turbulence models (renormalization group (RNG) k- $\epsilon$ , Reynolds stress model (RSM), and large-eddy simulation (LES)) are implemented, and the accuracy of each for predicting the hydrocyclone behavior is assessed. Four hydrocyclone configurations were analyzed using the RSM model. By analyzing the streamlines resulting from those simulations, it was found that the formation of some vortices and saddle points affect the separation efficiency. Furthermore, the effects of inlet width, cone length, and vortex finder diameter were found to be significant. The cut-size diameter was decreased by 33% compared to the Hsieh experimental hydrocyclone. An increase in the pressure drop leads to high values of cut-size and classification sharpness. If the pressure drop increases to twice its original value, the cut-size and the sharpness of classification are reduced to less than 63% and 55% of their initial values, respectively.

Type I pili are proteinaceous tethers that mediate bacterial adhesion of uropathogenic Escherichia coli to surfaces and are thought to help bacteria resist drag forces imparted by fluid flow via uncoiling of their quaternary structure. Uncoiling and recoiling have been observed in force spectroscopy experiments, but it is not clear if and how this process occurs under fluid flow. Here we developed an assay to study the mechanical properties of pili in a parallel plate flow chamber. We show that pili extend when attached E. coli bacteria are exposed to increasing shear stresses, that pili can help bacteria move against moderate fluid flows, and characterize two dynamic regimes of this displacement. The first regime is consistent with entropic contraction as modeled by a freely jointed chain, and the second with coiling of the quaternary structure of pili. These results confirm that coiling and uncoiling happen under flow but the observed dynamics are different from those reported previously. Using these results and those from previous studies, we review the mechanical properties of pili in the context of other elastic proteins such as the byssal threads of mussels. It has been proposed that the high extensibility of pili may help recruit more pili into tension and lower the force acting on each one by damping changes in force due to fluid flow. Our analysis of the mechanical properties suggests additional functions of pili; in particular, their extensibility may reduce tension by aligning pili with the direction of flow, and the uncoiled state of pili may complement uncoiling in regulating the force of the terminal adhesin.

Energy regeneration systems are a key factor for improving energy efficiency in electrohydraulic machinery. This paper is focused on the study of electric energy storage systems (EESS) and hydraulic energy storage systems (HESS) for energy regeneration applications. Two test benches were designed and implemented to compare the performance of the systems under similar operating conditions. The electrical system was configured with a set of ultracapacitors, and the hydraulic system used a hydraulic accumulator. Both systems were designed to have the same energy storage capacity. Charge and discharge cycle experiments were performed for the two systems in order to compare their power density, energy density, cost, and efficiency. According to the experimentally obtained results, the power density in the hydraulic accumulator was 21.7% higher when compared with the ultracapacitors. Moreover, the cost/power ($/Watt) ratio in the hydraulic accumulator was 2.9 times smaller than a set of ultracapacitors of the same energy storage capacity. On the other hand, the energy density in the set of ultracapacitors was 9.4 times higher, and the cost/energy ($/kWh) ratio was 2.9 times smaller when compared with the hydraulic accumulator. Under the tested conditions, the estimated overall energy efficiency for the hydraulic accumulator was 87.7%, and the overall energy efficiency for the ultracapacitor was 78.7%.

In this paper we present the design of an open-source and low-cost buoy prototype for remote monitoring of water quality variables in fish farming. The designed battery-powered system periodically measures temperature, pH and dissolved oxygen, transmitting the information locally through a low-power wide-area network protocol to a gateway connected to a cloud service for data storage and visualization. We provide a novel buoy design that can be easily constructed with off-the-shelf materials, delivering a stable anchored float for the IoT device and the probes immersed in the water pond. The prototype was tested at an operating fish farm, showing promising results for a low-cost remote monitoring tool that enables automatic data acquisition and storage in fish farming scenarios. All the elements of this design, including hardware and software designs, are freely available under permissive licenses as an open-source project.

The study of vesicles in suspension is important to understand the complicated dynamics exhibited by cells in in vivo and in vitro. We developed a computer simulation based on the boundary-integral method to model the three dimensional gravity-driven sedimentation of charged vesicles towards a flat surface. The membrane mechanical behavior was modeled using the Helfrich Hamiltonian and near incompressibility of the membrane was enforced via a model which accounts for the thermal fluctuations of the membrane. The simulations were verified and compared to experimental data obtained using suspended vesicles labelled with a fluorescent probe, which allows visualization using fluorescence microscopy and confers the membrane with a negative surface charge. The electrostatic interaction between the vesicle and the surface was modeled using the linear Derjaguin approximation for a low ionic concentration solution. The sedimentation rate as a function of the distance of the vesicle to the surface was determined both experimentally and from the computer simulations. The gap between the vesicle and the surface, as well as the shape of the vesicle at equilibrium were also studied. It was determined that inclusion of the electrostatic interaction is fundamental to accurately predict the sedimentation rate as the vesicle approaches the surface and the size of the gap at equilibrium, we also observed that the presence of charge in the membrane increases its rigidity.