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G protein γ subunit qPE9-1 plays multiple roles in rice growth and development. However, the role of qPE9-1 in rice exposed to elevated carbon dioxide concentration (eCO2) is unknown. Here, we investigated its role in the regulation of rice growth under eCO2 conditions using qPE9-1 overexpression (OE) lines, RNAi lines and corresponding WT rice. Compared to atmospheric carbon dioxide concentration (aCO2), relative expression of qPE9-1 in rice leaf was approximately tenfold higher under eCO2. Under eCO2, the growth of WT and qPE9-1-overexpressing rice was significantly higher than under aCO2. Moreover, there was no significant effect of eCO2 on the growth of qPE9-1 RNAi lines. Furthermore, WT and qPE9-1-overexpressing rice showed higher net photosynthetic rate and carbohydrate content under eCO2 than under aCO2. Moreover, the relative expression of some photosynthesis related genes in WT, but not in RNAi3 line, showed significant difference under eCO2 in RNA-seq analysis. Compared to WT and RNAi lines, the rbcL gene expression and Rubisco content of rice leaves in qPE9-1-overexpressors were higher under eCO2. Overall, these results suggest that qPE9-1 is involved in rice adaptation under elevated CO2 concentration by regulating leaf photosynthesis via moderating rice photosynthetic light reaction and Rubisco content.
Ke Wang; Feiyun Xu; Wei Yuan; Leyun Sun; Shaoxian Wang; Mehtab Muhammad Aslam; Jianhua Zhang; Weifeng Xu. G protein γ subunit qPE9-1 is involved in rice adaptation under elevated CO2 concentration by regulating leaf photosynthesis. Rice 2021, 14, 1 -10.
AMA StyleKe Wang, Feiyun Xu, Wei Yuan, Leyun Sun, Shaoxian Wang, Mehtab Muhammad Aslam, Jianhua Zhang, Weifeng Xu. G protein γ subunit qPE9-1 is involved in rice adaptation under elevated CO2 concentration by regulating leaf photosynthesis. Rice. 2021; 14 (1):1-10.
Chicago/Turabian StyleKe Wang; Feiyun Xu; Wei Yuan; Leyun Sun; Shaoxian Wang; Mehtab Muhammad Aslam; Jianhua Zhang; Weifeng Xu. 2021. "G protein γ subunit qPE9-1 is involved in rice adaptation under elevated CO2 concentration by regulating leaf photosynthesis." Rice 14, no. 1: 1-10.
Abscisic acid (ABA) plays an important role in plant adaptation to water deficits, but its role in regulating root growth (primary root elongation and lateral root number) during different drought-phases remains unclear. Here, we exposed wild-type (WT) and ABA-deficient (not) tomato plants to three continuous drought-phases (moderate drying: day 0–21; severe drying: day 22–47 and re-watering: day 48–51). It was found that WT increased primary root growth during moderate drying; maintained more lateral roots, and greater primary root and total root length under severe drying; and produced more roots after re-watering. After RNA-Seq analysis, we found that the auxin-related genes in root showed different expression patterns between WT and not under drying or re-watering. Further, exogenous supply of IAA partially recovered the root growth of ABA-deficient not plants under three continuous drought-phases. Our results suggested that ABA regulation of tomato root growth during soil drying and recovery can involve auxin response.
Qian Zhang; Wei Yuan; Qianwen Wang; Yiying Cao; Feiyun Xu; Ian C. Dodd; Weifeng Xu. ABA regulation of root growth during soil drying and recovery can involve auxin response. Plant, Cell & Environment 2021, 1 .
AMA StyleQian Zhang, Wei Yuan, Qianwen Wang, Yiying Cao, Feiyun Xu, Ian C. Dodd, Weifeng Xu. ABA regulation of root growth during soil drying and recovery can involve auxin response. Plant, Cell & Environment. 2021; ():1.
Chicago/Turabian StyleQian Zhang; Wei Yuan; Qianwen Wang; Yiying Cao; Feiyun Xu; Ian C. Dodd; Weifeng Xu. 2021. "ABA regulation of root growth during soil drying and recovery can involve auxin response." Plant, Cell & Environment , no. : 1.
Phosphorus (P) deficiency largely restricts plant growth and lead to severe yield losses. Therefore, identification of novel root traits to improve P uptake is needed to circumvent yield losses. White lupin (Lupinus albus) is a legume crop that develops cluster roots and has the high phosphorus use efficiency in low P soils. We aimed to investigate the association between cluster roots (CR) rhizosheath formation and P uptake in white lupin. Rhizosheath formation and P concentration were evaluated under four soil treatments. CR increased up to 2.5-fold of overall plant dry weight under SD–P compared to WW + P (control), partly attributable to variations in CR development. Our data showed that SD–P significantly increase rhizosheath weight in white lupin. Among the root segments, MCR showed improved P accumulation in the root which is associated with increased MCR rhizosheath weight. Additionally, a positive correlation was observed between MCR rhizosheath weight and P uptake. Moreover, high sucrose content was recorded in MCR, which may contribute in CR growth under SD–P. Expression analysis of genes related to sucrose accumulation (LaSUC1, LaSUC5, and LaSUC9) and phosphorus uptake (LaSPX3, LaPHO1, and LaPHT1) exhibited peaked expression in MCR under SD-P. This indicate that root sucrose status may facilitate P uptake under P starvation. Together, the ability to enhance P uptake of white lupin is largely associated with MCR rhizosheath under SD–P. Our results showed that gene expression modulation of CR forming plant species, demonstrating that these novel root structures may play crucial role in P acquisition from the soil. Our findings could be implicated for developing P and water efficient crop via CR development in sustainable agriculture.
Mehtab Muhammad Aslam; Joseph K. Karanja; Wei Yuan; Qian Zhang; Jianhua Zhang; Weifeng Xu. Phosphorus uptake is associated with the rhizosheath formation of mature cluster roots in white lupin under soil drying and phosphorus deficiency. Plant Physiology and Biochemistry 2021, 166, 531 -539.
AMA StyleMehtab Muhammad Aslam, Joseph K. Karanja, Wei Yuan, Qian Zhang, Jianhua Zhang, Weifeng Xu. Phosphorus uptake is associated with the rhizosheath formation of mature cluster roots in white lupin under soil drying and phosphorus deficiency. Plant Physiology and Biochemistry. 2021; 166 ():531-539.
Chicago/Turabian StyleMehtab Muhammad Aslam; Joseph K. Karanja; Wei Yuan; Qian Zhang; Jianhua Zhang; Weifeng Xu. 2021. "Phosphorus uptake is associated with the rhizosheath formation of mature cluster roots in white lupin under soil drying and phosphorus deficiency." Plant Physiology and Biochemistry 166, no. : 531-539.
The hab1-1abi1-2abi2-2pp2ca-1 quadruple mutant (Qabi2-2) seedlings lacking key negative regulators of ABA signaling, namely, clade A protein phosphatases type 2C (PP2Cs), show more apoplastic H+ efflux in roots and display an enhanced root growth under normal medium or water stress medium compared to the wild type. The presence of low ABA concentration (0.1 micromolar), inhibiting PP2C activity via monomeric ABA receptors, enhances root apoplastic H+ efflux and growth of the wild type, resembling the Qabi2-2 phenotype in normal medium. Qabi2-2 seedlings also demonstrate increased hydrotropism compared to the wild type in obliquely-oriented hydrotropic experimental system, and asymmetric H+ efflux in root elongation zone is crucial for root hydrotropism. Moreover, we reveal that Arabidopsis ABA-insensitive 1, a key PP2C in ABA signaling, interacts directly with the C terminus of Arabidopsis plasma membrane H+-dependent adenosine triphosphatase 2 (AHA2) and dephosphorylates its penultimate threonine residue (Thr947), whose dephosphorylation negatively regulates AHA2.
Rui Miao; Wei Yuan; Yue Wang; Irene Garcia-Maquilon; Xiaolin Dang; Ying Li; Jianhua Zhang; Yiyong Zhu; Pedro L. Rodriguez; Weifeng Xu. Low ABA concentration promotes root growth and hydrotropism through relief of ABA INSENSITIVE 1-mediated inhibition of plasma membrane H+-ATPase 2. Science Advances 2021, 7, eabd4113 .
AMA StyleRui Miao, Wei Yuan, Yue Wang, Irene Garcia-Maquilon, Xiaolin Dang, Ying Li, Jianhua Zhang, Yiyong Zhu, Pedro L. Rodriguez, Weifeng Xu. Low ABA concentration promotes root growth and hydrotropism through relief of ABA INSENSITIVE 1-mediated inhibition of plasma membrane H+-ATPase 2. Science Advances. 2021; 7 (12):eabd4113.
Chicago/Turabian StyleRui Miao; Wei Yuan; Yue Wang; Irene Garcia-Maquilon; Xiaolin Dang; Ying Li; Jianhua Zhang; Yiyong Zhu; Pedro L. Rodriguez; Weifeng Xu. 2021. "Low ABA concentration promotes root growth and hydrotropism through relief of ABA INSENSITIVE 1-mediated inhibition of plasma membrane H+-ATPase 2." Science Advances 7, no. 12: eabd4113.
Soil drying enhances root ABA accumulation and rhizosheath formation, but whether ABA mediates rhizosheath formation is unclear. Here, we used the ABA‐deficient mutant Az34 to investigate molecular and morphological changes by which ABA could affect rhizosheath formation. Mild soil drying with intermittent watering increased rhizosheath formation by promoting root and root hair elongation. Attenuated root ABA accumulation in Az34 barley constrained the promotion of root length and root hair length by drying soil, such that Az34 had a smaller rhizosheath. Pharmacological experiments of adding fluridone (an ABA biosynthesis inhibitor) and ABA to drying soil restricted and enhanced rhizosheath formation respectively in Az34 and wild‐type Steptoe barley. RNA sequencing suggested that ABA accumulation mediates auxin synthesis and responses and root and root hair elongation in drying soil. In addition, adding indole‐3‐acetic acid (IAA) to drying soil increased rhizosheath formation by promoting root and root hair elongation in Steptoe and Az34 barley. Together, these results show that ABA accumulation induced by mild soil drying enhance barley rhizosheath formation, which may be achieved through promoting auxin response.
Yingjiao Zhang; Feiyun Xu; Yexin Ding; Huan Du; Qian Zhang; Xiaolin Dang; Yiying Cao; Ian C. Dodd; Weifeng Xu. Abscisic acid mediates barley rhizosheath formation under mild soil drying by promoting root hair growth and auxin response. Plant, Cell & Environment 2021, 44, 1935 -1945.
AMA StyleYingjiao Zhang, Feiyun Xu, Yexin Ding, Huan Du, Qian Zhang, Xiaolin Dang, Yiying Cao, Ian C. Dodd, Weifeng Xu. Abscisic acid mediates barley rhizosheath formation under mild soil drying by promoting root hair growth and auxin response. Plant, Cell & Environment. 2021; 44 (6):1935-1945.
Chicago/Turabian StyleYingjiao Zhang; Feiyun Xu; Yexin Ding; Huan Du; Qian Zhang; Xiaolin Dang; Yiying Cao; Ian C. Dodd; Weifeng Xu. 2021. "Abscisic acid mediates barley rhizosheath formation under mild soil drying by promoting root hair growth and auxin response." Plant, Cell & Environment 44, no. 6: 1935-1945.
Lingyin Weng; Maoxing Zhang; Ke Wang; Guanglei Chen; Ming Ding; Wei Yuan; Yiyong Zhu; Weifeng Xu; Feiyun Xu. “Potassium alleviates ammonium toxicity in rice by reducing its uptake through activation of plasma membrane H+-ATPase to enhance proton extrusion” [Plant Physiol. Biochem. 151, (June 2020) 429-437]. Plant Physiology and Biochemistry 2020, 155, 987 .
AMA StyleLingyin Weng, Maoxing Zhang, Ke Wang, Guanglei Chen, Ming Ding, Wei Yuan, Yiyong Zhu, Weifeng Xu, Feiyun Xu. “Potassium alleviates ammonium toxicity in rice by reducing its uptake through activation of plasma membrane H+-ATPase to enhance proton extrusion” [Plant Physiol. Biochem. 151, (June 2020) 429-437]. Plant Physiology and Biochemistry. 2020; 155 ():987.
Chicago/Turabian StyleLingyin Weng; Maoxing Zhang; Ke Wang; Guanglei Chen; Ming Ding; Wei Yuan; Yiyong Zhu; Weifeng Xu; Feiyun Xu. 2020. "“Potassium alleviates ammonium toxicity in rice by reducing its uptake through activation of plasma membrane H+-ATPase to enhance proton extrusion” [Plant Physiol. Biochem. 151, (June 2020) 429-437]." Plant Physiology and Biochemistry 155, no. : 987.
Hydrotropism is the directed growth of roots toward the water found in the soil. However, mechanisms governing interactions between hydrotropism and gravitropism remain largely unclear. In this study, we found that an air system and an agar–sorbitol system induced only oblique water-potential gradients; an agar–glycerol system induced only vertical water-potential gradients; and a sand system established both oblique and vertical water-potential gradients. We employed obliquely oriented and vertically oriented experimental systems to study hydrotropism in Arabidopsis and tomato plants. Comparative analyses using different hydrotropic systems showed that gravity hindered the ability of roots to search for obliquely oriented water, whilst facilitating roots’ search for vertically oriented water. We found that the gravitropism-deficient mutant aux1 showed enhanced hydrotropism in the oblique orientation but impaired root elongation towards water in the vertical orientation. The miz1 mutant exhibited deficient hydrotropism in the oblique orientation but normal root elongation towards water in the vertical orientation. Importantly, in contrast to miz1, the miz1/aux1 double mutant exhibited hydrotropic bending in the oblique orientation and attenuated root elongation towards water in the vertical orientation. Our results suggest that gravitropism is required for MIZ1-regulated root hydrotropism in both the oblique orientation and the vertical orientation, providing further insight into the role of gravity in root hydrotropism.
Ying Li; Wei Yuan; Luocheng Li; Hui Dai; Xiaolin Dang; Rui Miao; František Baluška; Herbert J Kronzucker; Congming Lu; Jianhua Zhang; Weifeng Xu. Comparative analysis reveals gravity is involved in the MIZ1-regulated root hydrotropism. Journal of Experimental Botany 2020, 71, 7316 -7330.
AMA StyleYing Li, Wei Yuan, Luocheng Li, Hui Dai, Xiaolin Dang, Rui Miao, František Baluška, Herbert J Kronzucker, Congming Lu, Jianhua Zhang, Weifeng Xu. Comparative analysis reveals gravity is involved in the MIZ1-regulated root hydrotropism. Journal of Experimental Botany. 2020; 71 (22):7316-7330.
Chicago/Turabian StyleYing Li; Wei Yuan; Luocheng Li; Hui Dai; Xiaolin Dang; Rui Miao; František Baluška; Herbert J Kronzucker; Congming Lu; Jianhua Zhang; Weifeng Xu. 2020. "Comparative analysis reveals gravity is involved in the MIZ1-regulated root hydrotropism." Journal of Experimental Botany 71, no. 22: 7316-7330.
Summary The role of amyloplasts in the interactions between hydrotropism and gravitropism has been previously described. However, the effect of light-dark on the interactions between the two tropisms remains unclear. Here, by developing a method that makes it possible to mimic natural conditions more closely than the conventional lab conditions, we show that hydrotropism is higher in wild-type Arabidopsis seedlings whose shoots are illuminated but whose roots are grown in the dark compared with seedlings that are fully exposed to light. Root gravitropism is substantially decreased because of the reduction of amyloplast content in the root tip with decreased gene expression in PGM1 (a key starch biosynthesis gene), which may contribute to enhanced root hydrotropism under darkness. Furthermore, the starch-deficient mutant pgm1-1 exhibits greater hydrotropism compared with wild-type. Our results suggest that amyloplast response and starch reduction occur under light-dark modulation, followed by decreased gravitropism and enhanced hydrotropism in Arabidopsis root.
Ying Li; Wei Yuan; Luocheng Li; Rui Miao; Hui Dai; Jianhua Zhang; Weifeng Xu. Light-Dark Modulates Root Hydrotropism Associated with Gravitropism by Involving Amyloplast Response in Arabidopsis. Cell Reports 2020, 32, 108198 .
AMA StyleYing Li, Wei Yuan, Luocheng Li, Rui Miao, Hui Dai, Jianhua Zhang, Weifeng Xu. Light-Dark Modulates Root Hydrotropism Associated with Gravitropism by Involving Amyloplast Response in Arabidopsis. Cell Reports. 2020; 32 (13):108198.
Chicago/Turabian StyleYing Li; Wei Yuan; Luocheng Li; Rui Miao; Hui Dai; Jianhua Zhang; Weifeng Xu. 2020. "Light-Dark Modulates Root Hydrotropism Associated with Gravitropism by Involving Amyloplast Response in Arabidopsis." Cell Reports 32, no. 13: 108198.
The nonrenewable nutrient phosphorus (P) has low mobility in soil and is often present in forms that are unavailable to crop plants. Alternate wetting and drying irrigation is one of the widely promoted water‐saving irrigation techniques and can increase mineral nutrient availability; however, its interaction with P in paddy fields remains unclear. We conducted a 4‐year field experiment to determine whether a specific regime of frequent alternate wetting and drying (FAWD15) irrigation and low P usage (P45) had synergistic effects on rice yield, rice growth, water use efficiency (WUE), agronomic P use efficiency (AEp), and soil residual fertilizer P. We found that compared to the continuously flooded (CF) irrigation, FAWD15 irrigation maintained rice yield under low P usage condition in a 4‐year field trial. Our results also showed that under low P usage, FAWD15 resulted in high AEp and WUE, and reduced residual fertilizer P. Further, FAWD15 irrigation increased labile P release and resulted in increased root growth by improving root sucrose distribution. These results suggest that FAWD15 irrigation can mitigate the effect of low P usage on rice grain yield by increasing soil P release and root growth in paddy fields.
Feiyun Xu; Tao Song; Ke Wang; Weifeng Xu; Guanglei Chen; Min Xu; Qian Zhang; Jianping Liu; Yiyong Zhu; Christopher Rensing; Jianhua Zhang; Wei Yuan. Frequent alternate wetting and drying irrigation mitigates the effect of low phosphorus on rice grain yield in a 4‐year field trial by increasing soil phosphorus release and rice root growth. Food and Energy Security 2020, 9, 1 .
AMA StyleFeiyun Xu, Tao Song, Ke Wang, Weifeng Xu, Guanglei Chen, Min Xu, Qian Zhang, Jianping Liu, Yiyong Zhu, Christopher Rensing, Jianhua Zhang, Wei Yuan. Frequent alternate wetting and drying irrigation mitigates the effect of low phosphorus on rice grain yield in a 4‐year field trial by increasing soil phosphorus release and rice root growth. Food and Energy Security. 2020; 9 (3):1.
Chicago/Turabian StyleFeiyun Xu; Tao Song; Ke Wang; Weifeng Xu; Guanglei Chen; Min Xu; Qian Zhang; Jianping Liu; Yiyong Zhu; Christopher Rensing; Jianhua Zhang; Wei Yuan. 2020. "Frequent alternate wetting and drying irrigation mitigates the effect of low phosphorus on rice grain yield in a 4‐year field trial by increasing soil phosphorus release and rice root growth." Food and Energy Security 9, no. 3: 1.
Proton (H+) fluxes in plant roots play critical roles in maintaining root growth and facilitating plant responses to multiple soil stresses, including fluctuations in nutrient supply, salt infiltration, and water stress. Soil mining for nutrients and water, rates of nutrient uptake, and the modulation of cell expansion all depend on the regulation of root H+ fluxes, particularly at the root apex, mediated primarily by the activity of plasma membrane (PM) H+-ATPases. Here, we summarize recent findings on the regulatory mechanisms of H+ fluxes at the root apex under three abiotic stress conditions – phosphate deficiency, salinity stress, and water deficiency – and present an integrated physiomolecular view of the functions of H+ fluxes in maintaining root growth in the acclimation to soil stress.
Wei Siao; Devrim Coskun; František Baluška; Herbert J. Kronzucker; Weifeng Xu. Root-Apex Proton Fluxes at the Centre of Soil-Stress Acclimation. Trends in Plant Science 2020, 25, 794 -804.
AMA StyleWei Siao, Devrim Coskun, František Baluška, Herbert J. Kronzucker, Weifeng Xu. Root-Apex Proton Fluxes at the Centre of Soil-Stress Acclimation. Trends in Plant Science. 2020; 25 (8):794-804.
Chicago/Turabian StyleWei Siao; Devrim Coskun; František Baluška; Herbert J. Kronzucker; Weifeng Xu. 2020. "Root-Apex Proton Fluxes at the Centre of Soil-Stress Acclimation." Trends in Plant Science 25, no. 8: 794-804.
The rhizosheath is a layer of soil around the root that provides a favorable environment for soil microbe enrichment and root growth. Rice (Oryza sativa) roots form rhizosheaths under moderate soil drying (MSD) conditions, but how the rhizosheath forms associations with microbes is unclear. To investigate rice rhizosheath formation under MSD, we employed a multiphasic approach, integrating data from high-throughput sequencing and root-bacteria interactions. Rice roots formed a pronounced rhizosheath under MSD, but not under continuous flooding regimens. Plant growth-promoting rhizobacteria of the Enterobacteriaceae were enriched in rhizosheaths of two different rice varieties, ‘Gaoshan 1’ (drought tolerant) and ‘Nipponbare’ (drought sensitive). RNA-sequencing analysis revealed that the ethylene pathway was induced in the rhizosheath-root system under MSD. Enterobacter aerogenes, a bacterium isolated from the rhizosheath, degrades the ethylene precursor 1-aminocyclopropane-1-carboxylate, thereby increasing rhizosheath formation. Furthermore, a 1-aminocyclopropane-1-carboxylate deaminase-deficient mutant of E. aerogenes failed to enhance rice rhizosheath formation. Our results suggest that root-bacteria associations substantially contribute to rhizosheath formation in rice under MSD conditions by mechanisms that involve the ethylene response. These data inform strategies to reduce water consumption in rice production, one of the most water-intensive human activities.
Yingjiao Zhang; Huan Du; Feiyun Xu; Yexin Ding; Yao Gui; Jianhua Zhang; Weifeng Xu. Root-Bacteria Associations Boost Rhizosheath Formation in Moderately Dry Soil through Ethylene Responses. Plant Physiology 2020, 183, 780 -792.
AMA StyleYingjiao Zhang, Huan Du, Feiyun Xu, Yexin Ding, Yao Gui, Jianhua Zhang, Weifeng Xu. Root-Bacteria Associations Boost Rhizosheath Formation in Moderately Dry Soil through Ethylene Responses. Plant Physiology. 2020; 183 (2):780-792.
Chicago/Turabian StyleYingjiao Zhang; Huan Du; Feiyun Xu; Yexin Ding; Yao Gui; Jianhua Zhang; Weifeng Xu. 2020. "Root-Bacteria Associations Boost Rhizosheath Formation in Moderately Dry Soil through Ethylene Responses." Plant Physiology 183, no. 2: 780-792.
Alternate wetting and drying (AWD) irrigation has been widely used as an efficient rice production method to obtain better yield without continuous flooding (CF) of the paddy field. However, how this practice affects gene expression to regulate rice physiology and morphology is largely unknown. In this study, we used two rice varieties, Nipponbare, a lowland rice cultivar, and Gaoshan 1, an upland cultivar, and found that root dry weight (RDW) and root oxidation activity (ROA) in both cultivars substantially increased in response to AWD. We then analyzed the differences in transcriptome profiles of their roots irrigated in AWD vs. CF conditions. AWD responsive genes are mainly involved in lignin biosynthetic pathway and phytohormone signal transduction pathway and belong mainly to bHLH, bZIP, NAC, WRKY, and HSF transcription factor families. We discussed how these differentially expressed genes may contribute to the morphological adaptations observed in roots exposed to AWD. This analysis also provides useful information to explain the similarities and differences in adaptation to AWD irrigation between the two rice ecotypes.
Tao Song; Debatosh Das; Feng Yang; Moxian Chen; Yuan Tian; Chaolin Cheng; Chao Sun; Weifeng Xu; Jianhua Zhang. Genome-wide transcriptome analysis of roots in two rice varieties in response to alternate wetting and drying irrigation. The Crop Journal 2020, 8, 586 -601.
AMA StyleTao Song, Debatosh Das, Feng Yang, Moxian Chen, Yuan Tian, Chaolin Cheng, Chao Sun, Weifeng Xu, Jianhua Zhang. Genome-wide transcriptome analysis of roots in two rice varieties in response to alternate wetting and drying irrigation. The Crop Journal. 2020; 8 (4):586-601.
Chicago/Turabian StyleTao Song; Debatosh Das; Feng Yang; Moxian Chen; Yuan Tian; Chaolin Cheng; Chao Sun; Weifeng Xu; Jianhua Zhang. 2020. "Genome-wide transcriptome analysis of roots in two rice varieties in response to alternate wetting and drying irrigation." The Crop Journal 8, no. 4: 586-601.
The tissue culture regeneration system of Lupinus albus has always been considered as recalcitrant material due to its genotype-dependent response and low regeneration efficiency that hamper the use of genetic engineering. Establishment of repeatable plant regeneration protocol is a prerequisite tool for successful application of genetic engineering. This aim of this study was to develop standardized, efficient protocol for successful shoot induction from cotyledonary node of white lupin. In this study, 5 day old aseptically cultured seedlings were used to prepare three explants (half cotyledonary node, HCN; whole cotyledonary node, WCN; and traditional cotyledonary node, TCN), cultured on four concentrations of M519 medium (M519, ½ M519, 1/3 M519, and ¼ M519), containing four carbohydrate sources (sucrose, fructose, maltose, and glucose), and stimulated with various combinations of KT (kinetin), and NAA (naphthalene acetic acid) for direct shoot regeneration. High frequency of 80% shoot regeneration was obtained on ½ M519 medium (KT 4.0 mg L−1 + NAA 0.1 mg L−1) by using HCN as an explant. Interestingly, combinations of (KT 4.0 mg L−1 + NAA 0.1 mg L−1 + BAP 1.67 mg L−1), and (KT 2.0 mg L−1 + NAA 0.1 mg L−1) showed similar shoot regeneration frequency of 60%. Augmentation of 0.25 g L−1 activated charcoal (AC) not only reduced browning effect but also improved shoot elongation. Among the all carbohydrate sources, sucrose showed the highest regeneration frequency with HCN. Additionally, 80% rooting frequency was recorded on ½ M519 containing IAA 1.0 mg L−1 + KT 0.1 mg L−1 (indole acetic acid) after 28 days of culturing. The present study describes establishment of an efficient and successful protocol for direct plant regeneration of white lupin from different cotyledonary nodes.
Mehtab Muhammad Aslam; Joseph K. Karanja; Qian Zhang; Huifeng Lin; Tianyu Xia; Kashif Akhtar; Jianping Liu; Rui Miao; Feiyun Xu; Weifeng Xu. In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes. Plants 2020, 9, 318 .
AMA StyleMehtab Muhammad Aslam, Joseph K. Karanja, Qian Zhang, Huifeng Lin, Tianyu Xia, Kashif Akhtar, Jianping Liu, Rui Miao, Feiyun Xu, Weifeng Xu. In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes. Plants. 2020; 9 (3):318.
Chicago/Turabian StyleMehtab Muhammad Aslam; Joseph K. Karanja; Qian Zhang; Huifeng Lin; Tianyu Xia; Kashif Akhtar; Jianping Liu; Rui Miao; Feiyun Xu; Weifeng Xu. 2020. "In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes." Plants 9, no. 3: 318.
White lupin (Lupinus albus) is a legume crop that develops cluster roots and has high phosphorus (P)-use efficiency (PUE) in low-P soils. Here, we assemble the genome of white lupin and find that it has evolved from a whole-genome triplication (WGT) event. We then decipher its diploid ancestral genome and reconstruct the three sub-genomes. Based on the results, we further reveal the sub-genome dominance and the genic expression of the different sub-genomes varying in relation to their transposable element (TE) density. The PUE genes in white lupin have been expanded through WGT as well as tandem and dispersed duplications. Furthermore, we characterize four main pathways for high PUE, which include carbon fixation, cluster root formation, soil-P remobilization, and cellular-P reuse. Among these, auxin modulation may be important for cluster root formation through involvement of potential genes LaABCG36s and LaABCG37s. These findings provide insights into the genome evolution and low-P adaptation of white lupin.
Weifeng Xu; Qian Zhang; Wei Yuan; Feiyun Xu; Mehtab Muhammad Aslam; Rui Miao; Ying Li; Qianwen Wang; Xing Li; Xin Zhang; Kang Zhang; Tianyu Xia; Feng Cheng. The genome evolution and low-phosphorus adaptation in white lupin. Nature Communications 2020, 11, 1 -13.
AMA StyleWeifeng Xu, Qian Zhang, Wei Yuan, Feiyun Xu, Mehtab Muhammad Aslam, Rui Miao, Ying Li, Qianwen Wang, Xing Li, Xin Zhang, Kang Zhang, Tianyu Xia, Feng Cheng. The genome evolution and low-phosphorus adaptation in white lupin. Nature Communications. 2020; 11 (1):1-13.
Chicago/Turabian StyleWeifeng Xu; Qian Zhang; Wei Yuan; Feiyun Xu; Mehtab Muhammad Aslam; Rui Miao; Ying Li; Qianwen Wang; Xing Li; Xin Zhang; Kang Zhang; Tianyu Xia; Feng Cheng. 2020. "The genome evolution and low-phosphorus adaptation in white lupin." Nature Communications 11, no. 1: 1-13.
The rhizosheath is known to be beneficial for drought resistance in many plants, but the regulation of rhizosheath formation in rice plants is unclear. Here, we investigate rhizosheath formation in different rice varieties and root hair mutants. Our results showed that moderate water stress in rice induced rhizosheath formation. The soil porosity and water content were higher in the rice rhizosheath than in the rice bulk soil under moderate water stress. Additionally, rhizosheath formation in short root hair mutants was lower than in wild-type rice under moderate water stress. Moreover, transcriptomic results indicated that abscisic acid (ABA) and auxin were involved in root and root hair responses in rhizosheath formation. Further, blocking ABA and auxin pathways in wild type and in rhl1-1, the shortest root hair mutant, rhizosheath formation and root hair length were significantly decreased under moderate water stress. However, wild type plants maintained a higher root ABA content, root basipetal auxin transport, root hair length, and amount of rhizosheath than did rhl1-1. Our results suggest that moderate water stress in rice induces rhizosheath formation by modulating the ABA and auxin responses to regulate root and root hair growth, which may be used to breed rice varieties resistant to drought.
Yingjiao Zhang; Huan Du; Yao Gui; Feiyun Xu; Jianping Liu; Jianhua Zhang; Weifeng Xu. Moderate water stress in rice induces rhizosheath formation associated with abscisic acid and auxin responses. Journal of Experimental Botany 2020, 71, 2740 -2751.
AMA StyleYingjiao Zhang, Huan Du, Yao Gui, Feiyun Xu, Jianping Liu, Jianhua Zhang, Weifeng Xu. Moderate water stress in rice induces rhizosheath formation associated with abscisic acid and auxin responses. Journal of Experimental Botany. 2020; 71 (9):2740-2751.
Chicago/Turabian StyleYingjiao Zhang; Huan Du; Yao Gui; Feiyun Xu; Jianping Liu; Jianhua Zhang; Weifeng Xu. 2020. "Moderate water stress in rice induces rhizosheath formation associated with abscisic acid and auxin responses." Journal of Experimental Botany 71, no. 9: 2740-2751.
Background Drought stress is one of the major abiotic stresses that restrict plant growth and development. 14–3-3 proteins have been validated to regulate many biological processes in plants. Previous research demonstrated that OsGF14b plays different roles in panicle and leaf blast resistance. In this study, we researched the function of OsGF14b in drought resistance in rice. Findings Here, we report that OsGF14b was strongly induced by soil drought stress. In comparison with wild type (WT), the osgf14b mutant exhibited improved resistance to drought and osmotic stress by changing the content of stress-relevant parameters, complementation of the osgf14b mutant restored the drought sensitivity to WT levels, whereas the OsGF14b-overexpression lines exhibited enhanced sensitivity to drought and osmotic stress. The osgf14b mutant plants were hypersensitive to abscisic acid (ABA), while the OsGF14b-overexpression plants showed reduced sensitivity to ABA. Furthermore, mutation and overexpression of OsGF14b affected the expression of stress-related genes under normal growth conditions and/or drought stress conditions. Conclusions We have demonstrated that OsGF14b is involved in the drought resistance of rice plants, partially in an ABA-dependent manner.
Jianping Liu; Xinjiao Sun; Wencheng Liao; Jianhua Zhang; Jiansheng Liang; Weifeng Xu. Involvement of OsGF14b Adaptation in the Drought Resistance of Rice Plants. Rice 2019, 12, 1 -7.
AMA StyleJianping Liu, Xinjiao Sun, Wencheng Liao, Jianhua Zhang, Jiansheng Liang, Weifeng Xu. Involvement of OsGF14b Adaptation in the Drought Resistance of Rice Plants. Rice. 2019; 12 (1):1-7.
Chicago/Turabian StyleJianping Liu; Xinjiao Sun; Wencheng Liao; Jianhua Zhang; Jiansheng Liang; Weifeng Xu. 2019. "Involvement of OsGF14b Adaptation in the Drought Resistance of Rice Plants." Rice 12, no. 1: 1-7.
Root and rhizosphere is important for phosphorus (P) uptake in rice plants. However, little is known about the detailed regulation of irrigation regimes, especially frequently alternate wetting and drying (FAWD), on P usage of rice plants. Here, we found that compared with normal water and P dose, FAWD with reduced P dose maintained the grain yield in two rice varieties. Compared to rice variety Gaoshan1, rice variety WufengyouT025 displayed a higher grain yield, shoot P content, rhizosphere acid phosphatase activity, abundance of bacteria and bacterial acid phosphatase gene of rhizosphere. Moreover, FAWD regime may increase the abundance of bacteria with acid phosphatase activity to release available phosphorus in rhizosphere, which associated with rice varieties. Our results suggest that an optimized management of irrigation and phosphorous application can enhance both water and phosphorus use efficiency without sacrificing yield, which may contribute significantly to sustainable agriculture production.
Yingjiao Zhang; Xiaoyun Wang; Feiyun Xu; Tao Song; Huan Du; Yao Gui; Min Xu; Yiying Cao; Xiaolin Dang; Christopher Rensing; Jianhua Zhang; Weifeng Xu. Combining Irrigation Scheme and Phosphorous Application Levels for Grain Yield and Their Impacts on Rhizosphere Microbial Communities of Two Rice Varieties in a Field Trial. Journal of Agricultural and Food Chemistry 2019, 67, 10577 -10586.
AMA StyleYingjiao Zhang, Xiaoyun Wang, Feiyun Xu, Tao Song, Huan Du, Yao Gui, Min Xu, Yiying Cao, Xiaolin Dang, Christopher Rensing, Jianhua Zhang, Weifeng Xu. Combining Irrigation Scheme and Phosphorous Application Levels for Grain Yield and Their Impacts on Rhizosphere Microbial Communities of Two Rice Varieties in a Field Trial. Journal of Agricultural and Food Chemistry. 2019; 67 (38):10577-10586.
Chicago/Turabian StyleYingjiao Zhang; Xiaoyun Wang; Feiyun Xu; Tao Song; Huan Du; Yao Gui; Min Xu; Yiying Cao; Xiaolin Dang; Christopher Rensing; Jianhua Zhang; Weifeng Xu. 2019. "Combining Irrigation Scheme and Phosphorous Application Levels for Grain Yield and Their Impacts on Rhizosphere Microbial Communities of Two Rice Varieties in a Field Trial." Journal of Agricultural and Food Chemistry 67, no. 38: 10577-10586.
As one of the most widely promoted effective irrigation strategies for rice, alternate wetting and drying (AWD) irrigation can not only reduce water use but also increase mineral nutrient use efficiency. In this research, we compared the differences in grain yield, grain quality, phosphorus use efficiency (PUE), and growth states of roots and shoots of lowland and upland rice cultivars that were subjected to different irrigation and phosphorus (P) fertilizer application treatments in a field study for two years. The irrigation treatments consisted of two irrigation regimes: continuously flooded (CF) and AWD irrigation and the P fertilizer treatments included three P rates, i.e., 0, 45, and 90 kg ha−1 (P0, P45, and P90, respectively). The results revealed that AWD irrigation led to an increase in grain yield and improved PUE of both rice varieties at P45. The roots were longer and deeper under AWD irrigation, which contributed to the higher grain yield and higher resource use efficiency obtained with this treatment. At the lower P rates, both rice types translocated more P from vegetative tissues to grains, which led to a better PUE. Molecular analysis show that plant hormones (IAA, gibberellins, cytokinins and ABA) and members of the OsPht1 family are also involved in the regulation of P homeostasis under AWD irrigation. Our results demonstrate that AWD irrigation can also enhance PUE for the rice in the field.
Tao Song; Feiyun Xu; Wei Yuan; Moxian Chen; Qijuan Hu; Yuan Tian; Jianhua Zhang; Weifeng Xu. Combining alternate wetting and drying irrigation with reduced phosphorus fertilizer application reduces water use and promotes phosphorus use efficiency without yield loss in rice plants. Agricultural Water Management 2019, 223, 105686 .
AMA StyleTao Song, Feiyun Xu, Wei Yuan, Moxian Chen, Qijuan Hu, Yuan Tian, Jianhua Zhang, Weifeng Xu. Combining alternate wetting and drying irrigation with reduced phosphorus fertilizer application reduces water use and promotes phosphorus use efficiency without yield loss in rice plants. Agricultural Water Management. 2019; 223 ():105686.
Chicago/Turabian StyleTao Song; Feiyun Xu; Wei Yuan; Moxian Chen; Qijuan Hu; Yuan Tian; Jianhua Zhang; Weifeng Xu. 2019. "Combining alternate wetting and drying irrigation with reduced phosphorus fertilizer application reduces water use and promotes phosphorus use efficiency without yield loss in rice plants." Agricultural Water Management 223, no. : 105686.
Anthurium andraeanum, an important ornamental flower, has to go through a growth-delaying period after transfer from tissue culture to soil, which requires time and extra costs. Furthermore, during this period, the plantlets are highly susceptible to bacterial infections, which results in impaired development and severe losses. Here, we aimed to address whether application of the endophytic fungus, Piriformospora indica protects the A. andraeanum root system during the critical propagation period, and whether P. indica reduce the mortality rate by stimulating the host's resistance against diseases. We demonstrate that P. indica shortens the recovery period of Anthurium, promotes growth and confers disease resistance. The beneficial effect of P. indica results in faster elongation of Anthurium roots early in the interaction. P. indica-colonized plants absorb more phosphorus and exhibit higher photosynthesis rates than uncolonized control plants. Moreover, higher activities of stress-related enzymes, of jasmonic acid levels and mRNA levels of jasmonic acid-responsive genes suggest that the fungus prepares the plant to respond more efficiently to potentially upcoming threats, including bacterial wilt. These results suggest that P. indica is a helpful symbiont for promoting Anthurium rooting and development. All our evidences are sufficient to support the disease resistance conferred by P. indica through the plant-fungal symbiosis. Furthermore, it implicates that P. indica has strong potential as bio-fertilizer for utilization in ornamental plant cultivation.
Hui-Feng Lin; Jun Xiong; Hui-Ming Zhou; Chang-Ming Chen; Fa-Zhuang Lin; Xu-Ming Xu; Ralf Oelmüller; Wei-Feng Xu; Kai-Wun Yeh. Growth promotion and disease resistance induced in Anthurium colonized by the beneficial root endophyte Piriformospora indica. BMC Plant Biology 2019, 19, 40 .
AMA StyleHui-Feng Lin, Jun Xiong, Hui-Ming Zhou, Chang-Ming Chen, Fa-Zhuang Lin, Xu-Ming Xu, Ralf Oelmüller, Wei-Feng Xu, Kai-Wun Yeh. Growth promotion and disease resistance induced in Anthurium colonized by the beneficial root endophyte Piriformospora indica. BMC Plant Biology. 2019; 19 (1):40.
Chicago/Turabian StyleHui-Feng Lin; Jun Xiong; Hui-Ming Zhou; Chang-Ming Chen; Fa-Zhuang Lin; Xu-Ming Xu; Ralf Oelmüller; Wei-Feng Xu; Kai-Wun Yeh. 2019. "Growth promotion and disease resistance induced in Anthurium colonized by the beneficial root endophyte Piriformospora indica." BMC Plant Biology 19, no. 1: 40.
The meiotic processes of most polyploid rice (Oryza sativa) are genetically abnormal, leading to low pollen fertility, which results in low seed set rates. Some polyploid meiosis stability (PMeS) lines with high seed set rates have been bred but their meiotic mechanisms remain unknown. In this study, we investigated the function of OsMND1 regulated polyploid rice meiosis. OsMND1 localized in the nucleus, and its expression level in panicles of PMeS line HN2026-4x was higher than in HN2026-2x and the other lines without the PMeS background. OsMND1’s overexpression improved pollen fertility and viability, early normal embryo development and the seed set rate in Balilla-4x. However, OsMND1 RNAi in PMeS line HN2026-4x impeded pollen and embryo development significantly. The results of chromosome behavior analyses indicated that OsMND1 participates in stabilizing meiosis by maintaining the balance of pairing, synapsis and recombination during early meiosis. Many univalent, trivalent, and even multivalent systems appeared in the OsMND1 RNAi line, resulting in the presence of many lagging chromosomes. The outcome indicated that OsMND1 plays a critical role in stabilizing meiosis, improving pollen fertility and reducing early embryo abortions, ultimately increasing the seed set rate. Additionally, OsMND1 affected some key meiosis-related gene expression levels. These results raise interesting issues in polyploid breeding theory and application, which require integrated solutions in the future.
Yuguo Xiong; Lu Gan; Yaping Hu; Wenchao Sun; Xue Zhou; Zhaojian Song; Xianhua Zhang; Yang Li; Zhifan Yang; Weifeng Xu; YuChi He; Detian Cai; Jianhua Zhang. OsMND1 regulates early meiosis and improves the seed set rate in polyploid rice. Plant Growth Regulation 2019, 87, 341 -356.
AMA StyleYuguo Xiong, Lu Gan, Yaping Hu, Wenchao Sun, Xue Zhou, Zhaojian Song, Xianhua Zhang, Yang Li, Zhifan Yang, Weifeng Xu, YuChi He, Detian Cai, Jianhua Zhang. OsMND1 regulates early meiosis and improves the seed set rate in polyploid rice. Plant Growth Regulation. 2019; 87 (2):341-356.
Chicago/Turabian StyleYuguo Xiong; Lu Gan; Yaping Hu; Wenchao Sun; Xue Zhou; Zhaojian Song; Xianhua Zhang; Yang Li; Zhifan Yang; Weifeng Xu; YuChi He; Detian Cai; Jianhua Zhang. 2019. "OsMND1 regulates early meiosis and improves the seed set rate in polyploid rice." Plant Growth Regulation 87, no. 2: 341-356.