The effects of poaching on wildlife have been widely studied in conservation biology and can be heterogeneous, particularly on ungulates. These effects can be estimated through different methodologies whose use depends on several conditions such as Flight-initiation distance (FID). Our objectives were: 1- to evaluate whether poaching affects the FID and group structure of a guanaco (Lama guanicoe) population in a high cold desert in San Juan (Argentina); 2- to assess whether habitat structure (slope and vegetation cover) influences FID and group structure in this population. The study area included a site with poaching (unprotected area), and a site without poaching (protected area). We recorded 100 groups of guanacos: 70 in the protected and 30 in the unprotected area. FID and group size were greater in the unprotected than in the protected area, whereas proportions of group categories (with offspring, without offspring and solitary) were similar between areas. Besides, in relation to habitat structure, FID increased when vegetation cover decreased. On the other hand, FID and group size were not affected by slope. Our study shows that guanacos respond to poaching pressure as do other ungulate species, and that other factors such as vegetation cover also affect this behavior. Managers should be aware when interpreting FID due to its relation to habitat structure; the guanaco appears to assume greater risk (lower FID) in areas with high vegetation cover.

Roads can affect animals as well as their habits at different levels. Avoidance behavior is a common response of animals to this type of perturbation, preventing access to areas rich in resources. The effects of roads on ungulates have not been studied in South America extensively, especially in arid environments. We have studied the space use by ungulates in relation to roads, using a dung heap count and camera traps. The aim was to evaluate whether paved road and unpaved road may have an effect on the spatial use of a low density population of guanacos (Lama guanicoe) in Ischigualasto Provincial Park, Argentina. We observed an increase in dung heap abundance in unpaved road as respect to paved road, this difference was larger for both. Besides, we recorded less individuals in paved road zones than in unpaved road zones. This showed that roads, especially paved roads, negatively affect the space used by guanacos. Our results are relevant to the management and conservation of animal populations in protected areas since spatial segregation due to the presence of roads may lead to the isolation of individuals. It is important to pay attention and further assess the effects that roads can have in the native fauna.

Although the creation of protected areas helps to protect biodiversity, it does not necessarily contribute to the reduction in some conflicts, such as livestock predation by large carnivores. We evaluated the presence of puma (Puma concolor) in a large ranch converted into a National Park and in surrounding rancher areas in the Dry Chaco of Argentina. Two years after livestock removal from the protected area, puma occupancy was associated with wild prey richness, which was greater in the park than in the neighboring ranches, and was negatively related to livestock presence. We also evaluated ranchers’ perceptions of puma presence and their tolerance to livestock predation. Ranchers showed a negative perception of puma presence and a low tolerance to livestock predation. Therefore, this study suggests that, while the creation of a protected area can improve both predator and prey densities, successful mitigation of human–predator conflict requires further strategies and interventions, like economic compensation and environmental education programs.