Viral erythrocytic necrosis (VEN) affects over 20 species of marine and anadromous fishes in the North Atlantic and North Pacific Oceans. However, the distribution and strain variation of its viral causative agent, erythrocytic necrosis virus (ENV), has not been well characterized within Pacific salmon. Here, metatranscriptomic sequencing of Chinook salmon revealed that ENV infecting salmon was closely related to ENV from Pacific herring, with inferred amino-acid sequences from Chinook salmon being 99% identical to those reported for herring. Sequence analysis also revealed 89 protein-encoding sequences attributed to ENV, greatly expanding the amount of genetic information available for this virus. High-throughput PCR of over 19,000 fish showed that ENV is widely distributed in the NE Pacific Ocean and was detected in 12 of 16 tested species, including in 27% of herring, 38% of anchovy, 17% of pollock, and 13% of sand lance. Despite frequent detection in marine fish, ENV prevalence was significantly lower in fish from freshwater (0.03%), as assessed with a generalized linear mixed effects model (p = 5.5 × 10⁻⁸). Thus, marine fish are likely a reservoir for the virus. High genetic similarity between ENV obtained from salmon and herring also suggests that transmission between these hosts is likely.

Synopsis: Many coastal marine species have discontinuous distributions or genetic breakpoints throughout their geographical ranges. These spatial and genetic disjunctions occur in species that span limited to broad dispersal potential. Thus, the mechanisms that underlie these disjunctions remain speculative or incompletely known, particularly on small spatial scales where long-term historical processes are unlikely to be the only mechanism contributing to disjunction. Rather, ecological or oceanographic factors may be important. To identify key drivers of coastal disjunctions, we reviewed publications investigating spatial and genetic disjunctions in coastal marine species and visually summarized where and why they are thought to occur. The most frequently cited mechanisms implicated in causing disjunctions include historical processes, oceanographic features, heterogeneous habitat, species introductions, and limited larval dispersal capacities. However, the relative importance of each of these processes varies depending on the spatial scales investigated. Furthermore, locations associated with disjunctions for a suite of species are typically associated with multiple processes that maintain these disjunctions. This study provides a non-exhaustive synthesis of disjunctions in coastal marine species by visualizing where they occur, exploring underlying mechanisms, and investigating biases in how the scientific community studies this phenomenon.

Many marine species exhibit range gaps, patchy distributions, or genetic disjunctions throughout their ranges. These discontinuities can result from various interacting mechanisms, though directly linking ecological and historical factors to observed distributions or genetic disjunctions often proves challenging. Intriguingly, substantial research has demonstrated that these spatial and genetic discontinuities frequently occur in species with long-lived planktonic larvae, which possess the capacity for extensive oceanic dispersal. This study investigates the population genetics of one such species, the bat star Patiria miniata . Despite its long-lived planktonic larval stage of six to ten weeks, P. miniata maintains both a range gap and a, geographically separate, strong genetic disjunction throughout its distribution from Alaska to Baja California. Utilizing low-coverage whole-genome sequencing of over 200 individuals collected throughout P. miniata ’s range between the early 2000s and 2023, we corroborate previous findings of a significant genetic disjunction across Queen Charlotte Sound, north of Vancouver Island. Additionally, we present new evidence of strong divergence at several genomic loci across an extensive range gap in Washington and Oregon, despite subtle genetic population structure at most loci here. Such differences may reflect more recent ecological or oceanographic barriers, rather than historical processes. Our results demonstrate that while marine invertebrate populations may appear panmictic based on genome-wide metrics of population structure alone, strong local selection for specific gene segments may be maintained in some populations. This research contributes to our understanding of the complex interplay between dispersal potential and local adaptation in marine ecosystems and highlights the importance of considering both genetic structure and finer-scale adaptation in coastal marine populations.