Obesity is a chronic disease with a high prevalence, now recognized as a global epidemic. Studies show a strong association between high body mass index (BMI) and various chronic diseases, including non-alcoholic fatty liver (NAFL), cardiovascular diseases, diabetes, musculoskeletal disorders, chronic kidney disease, and mental health issues. These conditions negatively impact quality of life and elevate healthcare costs (Boutari et al., 2022). Objective: This study explores exogenous lipase produced by the fungus Fusarium solani as a biotechnological alternative for aesthetic and health applications, particularly for women with excess abdominal fat. Methods: F. solani was cultivated on solid medium, followed by submerged and semi-solid fermentation. The lipolytic activity of the extracts was evaluated through pH and temperature tests, surfactant effects, and storage stability. Additionally, we assessed cell viability in normal and cancer cells, antioxidant activity, antimicrobial properties, and fat degradation. Results: Submerged fermentation with olive oil yielded Lipase F2, which exhibited significant lipolytic activity. Lipase F2 demonstrated stability at pH 8.0, maintaining an activity of 135.89 U/mg and a purity of 3.63. It remained active between 35 and 70°C, with optimal stability at 35°C, and showed resistance to various surfactants and ions. The enzyme degraded chicken fat from 10g to 0.89g in 96 hours. Conclusion: An enzymatic formulation with F. solani lipase is viable for intradermal and surface applications, proving to be safe and non-toxic for human use.